2,3-Butanedione monoxime increases sensitivity to Nikkomycin Z in the budding yeast Saccharomyces cerevisiae.

Abstract:

:Nikkomycin Z (NZ) is a competitive inhibitor of chitin synthase III in the yeast Saccharomyces cerevisiae. Myosin type II-deficient yeast strains (myo1) display a dramatic reduction in growth when chitin synthase III activity is inhibited by NZ, supporting the contention that actomyosin motility plays an important role in maintaining cell wall integrity. A proposed inhibitor of cortical actin polymerization in vitro, 2,3-butanedione monoxime (BDM), also inhibits growth of wild-type yeast strains at a concentration of 20 mM. In this study, we assayed for potential in vivo interplay between BDM-sensitive cell functions and cell wall chitin synthesis by testing for increased sensitivity to NZ during co-treatment with BDM at sub-inhibitory concentrations. Our results show that BDM can increase the sensitivity of yeast cells to Nikkomycin Z.

authors

Rivera-Molina FE,González-Crespo S,Maldonado-De la Cruz Y,Ortiz-Betancourt JM,Rodríguez-Medina JR

doi

10.1007/s11274-005-9028-x

subject

Has Abstract

pub_date

2006-03-01 00:00:00

pages

255-260

issue

3

eissn

0959-3993

issn

1573-0972

journal_volume

22

pub_type

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