Abstract:
:We characterised the anti-Vibrio parahaemolyticus (anti-V. parahaemolyticus) marine bacteria DIT09, DIT44 and DIT46 isolated from the intertidal mussel Perumytilus purpuratus. The 16S rRNA gene sequences identify a Pseudoalteromonas sp. that form a clade with P. prydzensis and P. mariniglutinosa. The strains produced bacteriostatic anti-V. parahaemolyticus agents during the exponential growth phase, which were also active against V. cholerae and V. anguillarum, but not on other Gram positive and Gram negative bacteria. Bacteriostatic agents could be permeated by analytic ultra-filtration with 3.5 kDa cut-off, partially precipitated with 70 and 90 % ammonium sulphate, but not extracted with ethyl acetate. Reverse-phase HPLC revealed the production of a set of 5-6 active compounds by each strain (elution from 20 to 40 % acetonitrile), with similar but non identical HPLC patterns. Additionally, V. parahaemolyticus was able to progressively overcome the inhibition of antibiotics in trypticase soy agar with Fe(III) 0.5 up to 2 mM, suggesting the involvement of a set of novel siderophore or active molecules targeted at different Fe-siderophore uptake systems. The overall findings suggest that Pseudoalteromonas sp. DIT strains produce a putatively novel class of bacteriostatic and probably amphiphilic anti-Vibrio agents, indicating the need for further studies with chemical purification followed by their structural and functional characterization. Finally, the crude cell-free extracts, as well as the strains incubated at 10(3) and 10(5) c.f.u./mL, did not cause mortality in Artemia franciscana nauplii, suggesting that these bacteria are serious candidates for further probiotic evaluations with shellfish and fish cultures.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Aranda CP,Valenzuela C,Barrientos J,Paredes J,Leal P,Maldonado M,Godoy FA,Osorio CGdoi
10.1007/s11274-012-1044-zsubject
Has Abstractpub_date
2012-06-01 00:00:00pages
2365-74issue
6eissn
0959-3993issn
1573-0972journal_volume
28pub_type
杂志文章abstract::In this work Talaromyces australis and Penicillium murcianum pigment production in liquid cultures and the cytotoxic effect of such pigments on skin model cells were studied. Response surface methodology (RSM) was used to optimize culture conditions aiming to increase pigment production in malt extract and peptone-glu...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-019-2738-2
更新日期:2019-10-12 00:00:00
abstract::Cobamides ('complete' corrinoids) are essential for organohalide-respiring bacteria because they act as cofactors of reductive dehalogenases (RDases). RDases are the key enzymes in organohalide respiration, a process relevant for environmental remediation. More than a decade ago, the unusual norpseudo-B12 was identifi...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s11274-017-2258-x
更新日期:2017-05-01 00:00:00
abstract::Shewanella colwelliana, a marine bacterium isolated in association with the oyster Crassostrea virginica, produces an abundant exopolysaccharide with potential commercial value as an adhesive under aqueous conditions. Its utilization of glucose was modulated by stoichiometric concentrations of yeast extract. In Brain ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00367663
更新日期:1994-09-01 00:00:00
abstract::The methylotrophic yeast Pichia pastoris is widely used in recombinant expression of eukaryotic proteins owing to the ability of post-translational modification, tightly regulated promoters, and high cell density fermentation. However, episomal plasmids for heterologous gene expression and the CRISPR/Cas9 system for g...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-019-2654-5
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journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00327964
更新日期:1994-11-01 00:00:00
abstract::The present investigation entails the immobilisation and characterisation of Escherichia coli MO1-derived carbonic anhydrase (CA) and its influence on the transformation of CO₂ to CaCO₃. CA was purified from MO1 using a combination of Sephadex G-75 and DEAE cellulose column chromatography, resulting in 4.64-fold purif...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-013-1343-z
更新日期:2013-10-01 00:00:00
abstract::The potential use of Sporosarcina pasteurii in possible biotechnological applications on a large scale (ground improvement, consolidation of building structures and ornamental stone, or in developing bio-materials for the building industry), is based on its ability to produce high amounts of carbonate in a short perio...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-015-1844-z
更新日期:2015-06-01 00:00:00
abstract::The establishment of safe and effective methods for controlling fungal disease is an urgent issue in agriculture and forestry. Microbiological control of plant disease is expected to achieve better results than use of chemically derived fungicides. This study aimed to establish Brevibacillus laterosporus JX-5 as a pot...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-015-1912-4
更新日期:2015-10-01 00:00:00
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journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00364677
更新日期:1996-03-01 00:00:00
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journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-007-9423-6
更新日期:2007-12-01 00:00:00
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journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-013-1581-0
更新日期:2014-05-01 00:00:00
abstract::Iron-oxidizing bacteria (FeOB) refers to a group of bacteria with the ability to exchange and accumulate divalent iron dissolved in water as trivalent iron inside and outside the bacterial cell. Most FeOB belong the largest bacterial phylum, Proteobacteria. Within this phylum, FeOB with varying physiology with regards...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s11274-018-2491-y
更新日期:2018-07-04 00:00:00
abstract::Filamentous fungi, isolated from the enriched surface of garden soil, were screened for α-galactosidase production after growing on wheat-bran/carbohydrate substrate. One isolate,Aspergillus niger, had the highest enzyme activity (5.1×10(-2) units/mg protein) at pH 5.0 and 50°C. Treatment of cowpea flour with the crud...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF01238789
更新日期:1992-11-01 00:00:00
abstract::The incidence of fluoroquinolone-resistant Shigella strains has risen rapidly, presumably in response to ciprofloxacin antibiotic stress. Understanding the molecular mechanisms underlying this resistance phenotype is critical to developing novel and effective therapeutic strategies. In this study, the frequency of cip...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-012-1190-3
更新日期:2013-02-01 00:00:00
abstract::Both β-proteobacterial aerobic ammonium-oxidizing bacteria (AOB) and anaerobic ammonium-oxidizing (ANAMMOX) bacteria were investigated in the hyporheic zone of a contaminated river in China containing high ammonium levels and low chemical oxygen demand. Fluorescence in-situ hybridization (FISH), denaturing gradient ge...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-012-1090-6
更新日期:2012-09-01 00:00:00
abstract::An alternative procedure to obtain the parameters of Monod's growth model in batch culture is presented. It is based on the integral kinetic analysis methodology, employs a one-dimensional Golden Section search optimization method and is implemented on a spread-sheet programme. The procedure is discussed in detail and...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF01198762
更新日期:1992-07-01 00:00:00
abstract::A chromosomal gene, required for nodule development on Phaseolus bean, was characterized from Rhizobium etli strain TAL182. MLC640 is a Tn5 insertion mutant of TAL182 which shows decreased motility in soft TY agar and is defective in nodule development. The site of Tn5 insertion in MLC640 mapped to a 3.6-kb EcoRI chro...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00367673
更新日期:1994-09-01 00:00:00
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journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-017-2323-5
更新日期:2017-08-01 00:00:00
abstract::At 5 g/l, ferulic acid, a plant cell-wall phenolic, severely repressed growth of the lignocellulose-degrading fungi Trichoderma harzianum, Chaetomium cellulolyticum, Phanaerochaete chrysosporium, Trametes versicolor and Pleurotus sajor-caju. At 0.5 g/l, howerver, it slightly stimulated growth of the latter two organis...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00327792
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journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-020-02989-x
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journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-014-1792-z
更新日期:2015-02-01 00:00:00
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journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-010-0579-0
更新日期:2011-06-01 00:00:00
abstract::Valsa mali var. mali (Vmm), is the predominant species of apple valsa canker in China. Modern analysis of genes involved in virulence or pathogenicity usually implicate gene expression analysis most often performed using real-time quantitative polymerase chain reaction (RT-qPCR). However, for relative gene expression ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-013-1320-6
更新日期:2013-09-01 00:00:00
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journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00
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journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-011-0846-8
更新日期:2012-02-01 00:00:00
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journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-017-2399-y
更新日期:2018-02-15 00:00:00
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journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
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更新日期:1992-12-01 00:00:00
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journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-012-1062-x
更新日期:2012-07-01 00:00:00
abstract::The present study deals with membrane-bound efflux pumps, MexAB-OprM and MexXY and their respective regulatory genes mexR, nalC, nalD and mexZ in multidrug resistant (MDR) Pseudomonas aeruginosa. Following antibiotic sensitivity testing and detection of various beta-lactamases, hyperexpression of efflux pump genes, me...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-018-2465-0
更新日期:2018-05-30 00:00:00
abstract::Quaternary ammonium salts (QASs) are ubiquitous in nature, being found in organisms ranging from microorganisms to vertebrates (e.g., glycine betaine, carnitine) where they have important cellular functions. QASs are also obtained by chemical synthesis. These compounds, due to their diverse chemical structure (e.g. mo...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章,评审
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更新日期:2021-01-11 00:00:00