Abstract：
:Here, three different suicide vectors were evaluated for the possibility of performing gene mutagenesis in strain OH11 using the chiA gene (accession number: DQ888611) as a new reporter. Suicide vector pEX18GM was selected, and it was successfully applied for disruption and in-frame deletions in the chiA gene in strain OH11, which was confirmed by PCR amplification and Southern hybridization. The chiA-deletion mutant OH11-3 did not have the ability to produce chitinase on chitine selection medium. Interestingly, the chiA-deletion mutants displayed wild-type antimicrobial activity against Saccharomyces cerevisiae, Magnaporthe grisea, Phytophthora capsici, Rhizoctonia solani, Sclerotinia sclerotiorum and Pythium ultimum. Our data suggest that chitinase might not be a unique lytic enzyme in controlling S. cerevisiae, M. grisea, P. capsici, and P. ultimum. R. solani, S. sclerotiorum. Also, suicide vector pEX18GM might be explored as a potential tool for gene deletions in L. enzymogenes, which will facilitate the molecular study of mechanisms of biological control in L. enzymogenes.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Qian G,Wang Y,Qian D,Fan J,Hu B,Liu Fdoi
10.1007/s11274-011-0846-8subject
Has Abstractpub_date
2012-02-01 00:00:00pages
549-57issue
2eissn
0959-3993issn
1573-0972journal_volume
28pub_type
杂志文章abstract：:Pseudomonas aeruginosa possesses an arcade of both cell-associated and extracellular cytotoxic virulence factors which are regulated by a multi-component quorum sensing system. Many research studies report success of lactonase in combating the pathogenicity of P. aeruginosa but delivery of lactonase remains a challeng...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-014-1645-9
更新日期：2014-08-01 00:00:00
abstract：:We studied the effects of quaternary bis-phosphonium and bis-ammonium salts of pyridoxine with lipophilic substituents on the survival and morphology of Staphylococcus aureus cells. We found that, while originating from the same base, they exhibit considerably different antimicrobial mechanisms. In the presence of Ca(...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-015-1969-0
更新日期：2016-01-01 00:00:00
abstract：:An α-amylase from a hyper-producing strain of Bacillus (sp. E2) was stable at 70°C for 30 min but was quickly inactivated at higher temperatures. In the presence of 10MM Ca(2+) and starch (20% w/v), however, the enzyme was stable at 90°C for 10 min and after 30 min at 100°C still retained 26% of its initial activity. ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00286380
更新日期：1995-09-01 00:00:00
abstract：:Experiments were conducted to evaluate the efficacy of calliterpenone, a natural plant growth promoter from a shrub Callicarpa macrophylla Vahl., in enhancing the growth and yield promoting effects of plant growth promoting rhizobacteria (PGPRs), in menthol mint (Mentha arvensis L).This study is based on our previous ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-012-1238-4
更新日期：2013-05-01 00:00:00
abstract：:The Cochliobolus genus consist of over 55 species among which the 5 most devastating are Cochliobolus carbonum, Cochliobolus heterostrophus, Cochliobolus miyabeanus, Crocus sativus and Cochliobolus lunatus causing damages in sorghum, wheat, rice, maize, cassava and soybean estimated at over 10 billion USD per annum wo...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s11274-018-2569-6
更新日期：2018-12-01 00:00:00
abstract：:Valsa mali var. mali (Vmm), is the predominant species of apple valsa canker in China. Modern analysis of genes involved in virulence or pathogenicity usually implicate gene expression analysis most often performed using real-time quantitative polymerase chain reaction (RT-qPCR). However, for relative gene expression ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1320-6
更新日期：2013-09-01 00:00:00
abstract：:Forty-four strains of Aspergillus, Penicillium, Fusarium, Trichoderma and Rhizopus were grown on a liquid medium containing glucose and cassava-root extract. All of the Aspergillus and Fusarium strains, eight out of 10 Penicillium strains and three of seven Trichoderma strains showed linamarase activity. No such activ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00361015
更新日期：1995-11-01 00:00:00
abstract：:The diastatic power of four 'improved' Nigerian sorghum cultivars was produced principally by α-amylolytic activity, unlike that of Nigerian Proctor barley which came principally from β-amylolytic activity. Free amino nitrogen levels and extracts of the sorghums were higher than those of barley when the sorghum was ma...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01201881
更新日期：1992-05-01 00:00:00
abstract：:The potential of three Serratia marcescens strains (CFFSUR-B2, CFFSUR-B3 and CFFSUR-B4) isolated from tropical regions in Mexico to inhibit the mycelial growth and conidial germination of Colletotrichum gloeosporioides, causal agent of fruit anthracnose, was evaluated. The ability of these strains to produce prodigios...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-011-0803-6
更新日期：2012-01-01 00:00:00
abstract：:Diarrhoea due toVibrio cholerae non-01 is common in Bangladesh. Four hundred and eighty samples, including plants, water, phytoplankton and sediment, were collected from five ponds in Dhaka every 15 days for one year.V. cholerae non-01 was isolated from 181 (38%) of the samples. Two peaks were evident: one in April an...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01195838
更新日期：1992-03-01 00:00:00
abstract：:Wetland degradation makes significant impacts on soil, and bacterial communities in soil are likely to respond to these impacts. The purpose of this study was to investigate the impacts of soil property, soil type and soil depth on bacterial community in different stages of soil degradation in the Zoige Wetland. Micro...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-011-0858-4
更新日期：2012-02-01 00:00:00
abstract：:Tomato (Lycopersicon esculentum) is important widely grown vegetable in India and its productivity is affected by bacterial wilt disease infection caused by Ralstonia solanacearum. To prevent this disease infection a study was conducted to isolate and screen effective plant growth promoting rhizobacteria (PGPR) antago...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-011-0975-0
更新日期：2012-04-01 00:00:00
abstract：:Biohydrogen is considered as one of the most promising energy alternatives considering the climate and energy crisis. The dark fermentative hydrogen production from xylose at extreme thermophilic condition (70 °C) using mixed culture was conducted in this study. The effects of initial pH values (ranged from 5.0 to 10....
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-016-2178-1
更新日期：2017-01-01 00:00:00
abstract：:Curious or skeptical friends often ask, "What and why is COBIOTECH? What is it doing?" After serving as president during its formative first years, I attempt here to provide information for the curious and allay concerns of the skeptics about COBIOTECH'S emerging role in biotechnology internationally. ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00452835
更新日期：1991-11-01 00:00:00
abstract：:Lactic acid bacteria (LAB) isolated from different sources (dairy products, fruits, fresh and fermented vegetables, fermented cereals) were screened for antimicrobial activity against other bacteria, including potential pathogens and food spoiling bacteria. Six strains have been shown to produce bacteriocins: Lactococ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-014-1671-7
更新日期：2014-09-01 00:00:00
abstract：:Biotransformations have gained extensive importance in practical use as a support for chemical synthesis or in the conversion of natural products. Biotransformations may present an enlargement, a sequential degradation or a specific modification of synthetic or natural compounds. The tools for biotransformations are p...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF02421497
更新日期：1992-12-01 00:00:00
abstract：:A sustainable alternative to improve yield and the nutritive value of forage is the use of plant growth-promoting bacteria (PGPB) that release nutrients, synthesize plant hormones and protect against phytopathogens (among other mechanisms). Azospirillum genus is considered an important PGPB, due to the beneficial effe...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-017-2251-4
更新日期：2017-04-01 00:00:00
abstract：:Prebiotic Fructooligosaccharides (FOS) escape metabolism in upper GI tract undergo microbial metabolism in colon and thereby influence the nature, type and number of intestinal microbiota to improve host's health. The present study focuses on the ability of Lactobacillus plantarum CFR 2194 to utilize FOS as a selectiv...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-011-0887-z
更新日期：2012-03-01 00:00:00
abstract：:An extracellular polygalacturonase (EC 3.2.1.15) fromGeotrichum candidum ATCC 34614 grown onsauerkraut brine was produced and characterized. Polygalacturonic acid markedly increased the enzyme yield in the brine. The fungus produced the highest activity (290 U/l) in brine with 0.3% (w/v) polygalacturonic acid. The pH ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01201944
更新日期：1992-09-01 00:00:00
abstract：:Anaerobic degradation of organic matter follows similar pathways in digesters and anaerobic freshwater sediments. The responsible microorganisms are linked in a complex food web, where short chain fatty acids and H2 are important intermediates. Degradation of short-chain fatty acids is endothermic under standard condi...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00419463
更新日期：1996-09-01 00:00:00
abstract：:The aims of this work were to identify in vivo manifestations of antioxidant activity of Lactobacillus strains isolated from healthy human biotopes and to show the possibility of protective action of the selected strain on the model of oxidative stress induced by paraquat in the model of early Parkinson's disease (PD)...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-020-02879-2
更新日期：2020-07-06 00:00:00
abstract：:Production of biogas in batch digesters at 30°C from sheep droppings produced 93 l gas/kg dry matter whereas cattle dung yielded 234 l/kg dry matter. When the sheep droppings and cattle dung were used at 25:75 (w/w), gas production per kg dry matter was equal to that of cattle dung only. The methane content of the bio...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00327830
更新日期：1993-03-01 00:00:00
abstract：:Species of the genus Trichoderma are economically important as biocontrol agents, serving as a potential alternative to chemical control. The applicability of Trichoderma isolates to different ecozones will depend on the behavior of the strains selected from each zone. The present study was undertaken to isolate bioco...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-015-1989-9
更新日期：2016-03-01 00:00:00
abstract：:An extracellular lipase producing isolate Staphylococcus sp. MS1 was optimized for lipase production and its biocatalytic potential was assessed. Medium with tributyrin (0.25 %) and without any exogenous inorganic nitrogen source was found to be optimum for lipase production from Staphylococcus sp. MS1. The optimum pH...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-014-1715-z
更新日期：2014-11-01 00:00:00
abstract：:Trehalose synthase (TreS) catalyzes the reversible interconversion of maltose and trehalose. A novel treS gene with a length of 3,369 bp, encoding a protein of 1,122 amino acid residues with a predicted molecular mass of 126 kDa, was cloned from a marine Pseudomonas sp. P8005 (CCTCC: M2010298) and expressed in Escheri...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1385-2
更新日期：2013-11-01 00:00:00
abstract：:Silver nanoparticles (SNPs) are extensively used in many industrial and medical applications; however, the impact of their release in the environment is still considered an understudied field. In the present work, SNPs present in aqueous lab waste water (average size of 30 nm) were used to determine their impact on mi...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-014-1597-0
更新日期：2014-06-01 00:00:00
abstract：:Cobamides ('complete' corrinoids) are essential for organohalide-respiring bacteria because they act as cofactors of reductive dehalogenases (RDases). RDases are the key enzymes in organohalide respiration, a process relevant for environmental remediation. More than a decade ago, the unusual norpseudo-B12 was identifi...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s11274-017-2258-x
更新日期：2017-05-01 00:00:00
abstract：:A xylanase gene, designated Svixyn10A, was cloned from actinomycetes Saccharomonospora viridis and the gene product was characterized. Gene Svixyn10A contains 1,374 bp and encodes a polypeptide of 457 amino acids composed of a glycoside hydrolase family 10 catalytic domain with a putative signal peptide, a short Gly-r...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-012-1085-3
更新日期：2012-08-01 00:00:00
abstract：:Cr(VI) is a highly toxic metal produced by anthropogenic activity which may impact the environment, affecting plants and animals. In plants, chromium both as Cr(III) or Cr(VI) can be absorbed by roots, is poorly translocated and affects negatively plant growth. Plants used in phytoremediation need to cope with chromiu...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-017-2389-0
更新日期：2017-12-18 00:00:00
abstract：:Pseudomonas strains were isolated from the rhizosphere of maize grown in yellow-red latosol from Rio de Janeiro, Brazil, to serve as a delivery system for heterologous genes and for risk assessment studies in tropical soils. Selected strains were modified by insertion of the cryIVB gene from Bacillus thuringiensis and...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00327720
更新日期：1996-11-01 00:00:00
