Abstract：
:Biohydrogen is considered as one of the most promising energy alternatives considering the climate and energy crisis. The dark fermentative hydrogen production from xylose at extreme thermophilic condition (70 °C) using mixed culture was conducted in this study. The effects of initial pH values (ranged from 5.0 to 10.0) and substrate concentrations (ranged from 2.5 to 15.0 g/L) on the hydrogen production, substrate degradation and metabolite distributions were investigated using batch-mode operations. Results showed that initial substrate pH values in the neutral region (6.0-7.0) were beneficial for hydrogen production. The fermentation at initial pH 7.0 and 7.5 g/L xylose reached an optimal hydrogen yield of 1.29 mol-H2/mol-xyloseconsumed. Ethanol, butyrate, and propionate were the major liquid metabolites. The xylose biodegradation efficiency of the mixed culture decreased sharply at high initial culture pH values. The increase of xylose concentration resulted in the accumulation of propionate and an obvious decrease in the final pH value, as well as a low hydrogen yield. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis indicated that hydrogen producing bacteria were enriched by repeated culture under extreme thermophilic conditions. Also, the mixed culture was dominated with bacterial species related to Clostridium and Thermoanaerobacterium.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Qiu C,Shi P,Xiao S,Sun Ldoi
10.1007/s11274-016-2178-1subject
Has Abstractpub_date
2017-01-01 00:00:00pages
7issue
1eissn
0959-3993issn
1573-0972pii
10.1007/s11274-016-2178-1journal_volume
33pub_type
杂志文章abstract：:Anabaena doliolum grown under molybdenum deficiency produced less biomass (on a dry wt basis) and the cells had lower protein content but higher carbohydrate content than Mo-grown material. Molybdenum deficiency led to a slight decrease in chlorophyll a, a 1.5-fold increase in carotenoids and a 1.4-fold increase in to...
journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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更新日期：2007-12-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2014-01-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章,评审
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更新日期：2017-05-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00328978
更新日期：1991-03-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2012-08-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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doi：10.1007/s11274-011-0836-x
更新日期：2012-02-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2020-10-21 00:00:00
