Purification and biochemical characterization of a superoxide dismutase from the soluble fraction of the cyanobacterium, Spirulina platensis.

Abstract:

:A superoxide dismutase (SOD) was purified from Spirulina platensis sonicate. The SOD was purified to homogeneity (48-fold and 0.24% yield) through ammonium sulphate precipitation and DEAE-52 anion exchange chromatography. The SOD from S. platensis appeared to be a homodimer with a molecular weight of 30 kDa and a subunit MW of 15 kDa as determined by both native polyacrylamide gel electrophoresis and mass spectrometry. The enzyme activity was stable at pH 6.5-10.0 and 50 °C. Using group-specific chemical modifying reagents, the amino acids arginine, histidine, tryptophan, tyrosine and aspartic acid were identified to be essential for S. platensis SOD activity. The amino acid composition was found to lack methionine and cysteine. The inhibition of activity by H2O2 suggests that the enzyme may be an iron containing SOD.

authors

Desai K,Sivakami S

doi

10.1007/s11274-007-9413-8

subject

Has Abstract

pub_date

2007-12-01 00:00:00

pages

1661-6

issue

12

eissn

0959-3993

issn

1573-0972

pii

10.1007/s11274-007-9413-8

journal_volume

23

pub_type

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