Abstract：
:Biomineralization is a process by which living organisms produce minerals. The extracellular production of these biominerals by microbes has potential for various bioengineering applications. For example, crack remediation and improvement of durability of concrete is an important goal for engineers and biomineral-producing microbes could be a useful tool in achieving this goal. Here we report the isolation, biochemical characterization and molecular identification of Pseudomonas azotoformans, a microbe that produces calcite and which potentially be used to repair cracks in concrete structures. Initially, 38 bacterial isolates were isolated from soil and cements. As a first test, the isolates were screened using a urease assay followed by biochemical tests for the rate of urea hydrolysis, calcite production and the insolubility of calcite. Molecular amplification and sequencing of a 16S rRNA fragment of selected isolates permitted us to identify P. azotoformans as a good candidate for preparation of biotechnological concrete. This species was isolated from soil and the results show that among the tested isolates it had the highest rate of urea hydrolysis, produced the highest amount of calcite, which, furthermore was the most adhesive and insoluble. This species is thus of interest as an agent with the potential ability to repair cracks in concrete.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Heidari Nonakaran S,Pazhouhandeh M,Keyvani A,Abdollahipour FZ,Shirzad Adoi
10.1007/s11274-015-1948-5subject
Has Abstractpub_date
2015-12-01 00:00:00pages
1993-2001issue
12eissn
0959-3993issn
1573-0972pii
10.1007/s11274-015-1948-5journal_volume
31pub_type
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pub_type： 杂志文章
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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journal_title：World journal of microbiology & biotechnology
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