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Space mutagenesis of genetically engineered bacteria expressing recombinant human interferon α1b and screening of higher yielding strains.

Abstract：

:The aim of this study was to investigate the space mutagenesis of genetically engineered bacteria expressing recombinant human interferon α1b. The genetically engineered bacteria expressing the recombinant interferon α1b were sent into outer space on the Chinese Shenzhou VIII spacecraft. After the 17 day space flight, mutant strains that highly expressed the target gene were identified. After a series of screening of spaceflight-treated bacteria and the quantitative comparison of the mutant strains and original strain, we found five strains that showed a significantly higher production of target proteins, compared with the original strain. Our results support the notion that the outer space environment has unique effects on the mutation breeding of microorganisms, including genetically engineered strains. Mutant strains that highly express the target protein could be obtained through spaceflight-induced mutagenesis.

journal_name

World J Microbiol Biotechnol

journal_title

World journal of microbiology & biotechnology

authors

Wang J,Liu C,Liu J,Fang X,Xu C,Guo Y,Chang D,Su L

doi

10.1007/s11274-013-1512-0

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

943-9

issue

3

eissn

0959-3993

issn

1573-0972

journal_volume

30

pub_type

杂志文章

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