Abstract：
:Elicitation is one of the few strategies that find commercial application in the enhancement of secondary metabolite production from plants as well as cell culture systems. Due to their immense medicinal value, production of saponins in suspension cultures has been attempted by many researchers. Gymnema sylvestre is a rich source of gymnemic acids (saponins) that find application in the treatment of diabetes. The present study is an attempt to evaluate the effect of various metal salts (cadmium chloride, mercuric chloride, silver nitrate, cupric chloride, cobaltous chloride and calcium chloride) in eliciting the response from G. sylvestre suspension cultures. The maximum gymnemic acid production in the suspensions was achieved on day 12 of culture, though the maximum biomass was obtained on day 16. Among the different salts, CdCl(2) gave maximum response (59.97 mg/gDCW) at 2 mM concentration after a 24 h time period, while, AgNO(3) gave the least response (18.35 mg/gDCW) on incubation of 48 h at 1 mM concentration, in terms of gymnemic acid accumulation. The accumulation of gymnemic acid was found to be dependent on treatment time and concentration of the elicitor. The enhanced gymnemic acid production shown by the suspensions in response to the metal salts indicates their role in evoking the plant defense mechanisms. These elicitation studies help in providing a platform for improved commercial supply of bioactive gymnemic acids.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Ch B,Rao K,Gandi S,Giri Adoi
10.1007/s11274-011-0870-8subject
Has Abstractpub_date
2012-02-01 00:00:00pages
741-7issue
2eissn
0959-3993issn
1573-0972journal_volume
28pub_type
杂志文章abstract：:A strain of Aspergillus niger isolated from sugarcane fields, produced an extracellular transfructosylase in the culture medium. Sucrose and raffinose induced the production to the enzyme, which was purified by 138-fold. The optimum pH for activity and stability were 5.5 and 6.5, respectively. Its optimum temperature ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00329399
更新日期：1991-05-01 00:00:00
abstract：:Mercury-resistance genes were detected byin situ hybridization using new synthetic oligonucleotide probes specific formerA andmerB genes according to the published sequences of the corresponding enzymes. These DNA probes were used for the detection of specific mercury-resistant microorganisms isolated from the Rhine R...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01200680
更新日期：1992-01-01 00:00:00
abstract：:An analytical procedure has been developed for at-line (fast off-line) monitoring of 4 key parameters including nisin titer (NT), the concentration of reducing sugars, cell concentration and pH during a nisin fermentation process. This procedure is based on near infrared (NIR) spectroscopy and Partial Least Squares (P...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-011-0897-x
更新日期：2012-03-01 00:00:00
abstract：:Valsa mali var. mali (Vmm), is the predominant species of apple valsa canker in China. Modern analysis of genes involved in virulence or pathogenicity usually implicate gene expression analysis most often performed using real-time quantitative polymerase chain reaction (RT-qPCR). However, for relative gene expression ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1320-6
更新日期：2013-09-01 00:00:00
abstract：:The diastatic power of four 'improved' Nigerian sorghum cultivars was produced principally by α-amylolytic activity, unlike that of Nigerian Proctor barley which came principally from β-amylolytic activity. Free amino nitrogen levels and extracts of the sorghums were higher than those of barley when the sorghum was ma...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01201881
更新日期：1992-05-01 00:00:00
abstract：:Kaolin is an important industrial raw material and a basis of a range of different products. Microbial spoilage is a detrimental process observed especially in kaolin slurries, leading to low quality products and economic loss. Although the alteration of kaolin slurries in ceramic industry was observed, the process an...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-011-0976-z
更新日期：2012-04-01 00:00:00
abstract：:Trichoderma harzianum produced RNA-depolymerase with maximum activity after 72 to 120 h of growth. Addition of K2HPO4 repressed enzyme production by the fungus. The optimal activity was at pH 7.8 and 40 to 50°C. The enzyme was stable at pH 3.2 to 9.0 and 80% of activity remained after 60 min at 40°C. EDTA and p-chloro...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00383067
更新日期：1993-05-01 00:00:00
abstract：:Production of biogas in batch digesters at 30°C from sheep droppings produced 93 l gas/kg dry matter whereas cattle dung yielded 234 l/kg dry matter. When the sheep droppings and cattle dung were used at 25:75 (w/w), gas production per kg dry matter was equal to that of cattle dung only. The methane content of the bio...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00327830
更新日期：1993-03-01 00:00:00
abstract：:The role of S-layer proteins (SLP) on the Pb(2+) sequestrant capacity by Lactobacillus kefir CIDCA 8348 and JCM 5818 was investigated. Cultures in the stationary phase were treated with proteinase K. A dot blot assay was carried out to assess the removal of SLP. Strains with and without SLP were exposed to 0-0.5 mM Pb...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-015-1812-7
更新日期：2015-04-01 00:00:00
abstract：:Screening for spore-forming bacteria to use against dipterous insects, namelyMusca domestica, Aedes aegypti andCulex pipiens from Egypt, led to two isolates, M1 and S128, being found which were highly toxic to bothAedes andCulex strains. They had calculated international Toxicity Units (ITU) valdes of 17,467 and 20,06...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01201301
更新日期：1990-09-01 00:00:00
abstract：:This paper aims to characterize halophilic bacteria inhabiting Algerian Saline Ecosystems (Sebkha and Chott) located in arid and semi-arid ecoclimate zones (Northeastern Algeria). In addition, screening of enzymatic activities, heavy metal tolerance and antagonistic potential against phytopathogenic fungi were tested....
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-019-2705-y
更新日期：2019-08-20 00:00:00
abstract：:A strain of Pleurotus ostreatus was grown in tomato pomace as sole carbon source for production of laccase. The culture of P. ostreatus revealed a peak of laccase activity (147 U/L of fermentation broth) on the 4th day of culture with a specific activity of 2.8 U/mg protein. Differential chromatographic behaviour of l...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-011-0813-4
更新日期：2012-01-01 00:00:00
abstract：:Grain yields of the rice cultivar 'Prakash' were improved upon inoculation with Glomus intraradices and G. fasciculatum, by 11% and 8%, respectively, compared with an uninoculated control. The results indicate that the amount of phosphate fertilizer usually applied to rice may be decreased by 50%, without affecting yi...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00144455
更新日期：1994-07-01 00:00:00
abstract：:Higher Basidiomycota have been used in natural medicine throughout the world for centuries. One of such fungi is Fomitopsis betulina (formerly Piptoporus betulinus), which causes brown rot of birch wood. Annual white to brownish fruiting bodies of the species can be found on trees in the northern hemisphere but F. bet...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s11274-017-2247-0
更新日期：2017-05-01 00:00:00
abstract：:The success of biotechnological research, development and marketing depends to a large extent on the international transfer of information and on the ability to organise biotechnology information into knowledge. To increase the efficiency of information-based approaches, an information strategy has been developed and ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00419453
更新日期：1996-09-01 00:00:00
abstract：:A symbiotic, heterocystous, N2-fixing blue-green alga, isolated from the coralloid roots of a xerophytic plant,Cycas revoluta, grew best in liquid medium supplemented with 4 mM NO 3 (-) . Morphologically, the isolated alga was identical to that of the natural endophyte but the cell size had decreased markedly. The alg...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01201954
更新日期：1992-09-01 00:00:00
abstract：:The mushroom Flammulina velutipes and the white-rot fungus Trametes versicolor were cultivated separately on sugarcane bagasse for 40 days. Trametes versicolor produced laccase and manganese-peroxidase activities, showing a simultaneous degradation of lignin and holocellulose. However, only phenoloxidase activity was ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00286370
更新日期：1995-09-01 00:00:00
abstract：:An inhibitory factor (IF) produced byAspergillus niger strain 19, and which inhibits the action of glucoamylase on starch, has the ability to be tightly adsorbed on to various raw starches, though the amount differs from starch to starch. Based on the hydrolysis of the IF-starch complex by glucoamylase, the inhibition...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01202122
更新日期：1990-12-01 00:00:00
abstract：:Lateritic nickel ore from the Sukinda Mines, Orissa, India, was leached using Thiobacillus ferrooxidans, Bacillus circulans, Bacillus licheniformis and Aspergillus niger at 5% (w/v) solid: liquid ratio for 5-20 days. Maximum leaching of Ni was achieved with B. circulans (85%) and Aspergillus niger (92%) after 20 days....
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00327850
更新日期：1993-03-01 00:00:00
abstract：:Aureobasidium pullulans NRRL 6220 synthesized polysaccharide most actively in media containing sucrose, fructose or maltose with (NH4)2SO4 (0.6 g/l) or ammonium acetate giving greatest yields of the polysaccharide. With (NH4)2SO4 at ≥1.2 g/l, production of polysaccharide was decreased considerably. Polysaccharide prod...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00144465
更新日期：1994-07-01 00:00:00
abstract：:Aquilaria malaccensis produces agarwood in response to wounding and fungal attack. However, information is limited regarding Aquilaria's interaction with its diverse fungal community. In this study, time-related changes of three natural fungal colonizers in two wounded wild A. malaccensis were tracked, beginning a few...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-014-1668-2
更新日期：2014-09-01 00:00:00
abstract：:Trichoderma asperellum parasitizes a large variety of phytopathogenic fungi. The mycoparasitic activity of T. asperellum depends on the secretion of complex mixtures of hydrolytic enzymes able to degrade the host cell wall and proteases which are a group of enzymes capable of degrading proteins from host. In this stud...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1373-6
更新日期：2013-11-01 00:00:00
abstract：:Opuntia ficus-indica Mill. (forage cactus) is farmed with relative success in the semi-arid region of the Brazilian northeast for commercial purposes, particularly as forage and food. Endophytic microorganisms are those that can be isolated inside plant tissues and can be a new source to production of enzymes with dif...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-011-1001-2
更新日期：2012-05-01 00:00:00
abstract：:Cabernet Sauvignon grape produced in Xinjiang (China) is often overripe, with unusually high sugar content, which impedes utilization. We aimed to establish the optimal combination of indigenous yeast strains to produce a new sweet wine from the overripe grape. Five yeast strains with pronounced enological characteris...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-020-02831-4
更新日期：2020-07-20 00:00:00
abstract：:The incidence of fluoroquinolone-resistant Shigella strains has risen rapidly, presumably in response to ciprofloxacin antibiotic stress. Understanding the molecular mechanisms underlying this resistance phenotype is critical to developing novel and effective therapeutic strategies. In this study, the frequency of cip...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-012-1190-3
更新日期：2013-02-01 00:00:00
abstract：:Tandem repeats of single short peptide sequences are useful for many purposes. Here we describe a method called ligation-PCR to construct DNA sequences encoding numerous tandem peptide repeats that can stably produce such repeats in both prokaryotic and eukaryotic cells. The method employs double-strand target monomer...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-012-1023-4
更新日期：2012-05-01 00:00:00
abstract：:Identification of Clostridium botulinum is usually based on toxin detection of broth culture by mouse bioassay and requires 7 to 10 days to complete. Here, we describe an alternative in vitro procedure for direct identification of C. botulinum (types A and B) colonies which can be completed in 48 h. The method is base...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00357557
更新日期：1994-01-01 00:00:00
abstract：:Butanol is not only an important chemical intermediate and solvent in pharmaceutical and cosmetics industries, but also considered as an advanced biofuel. Although species of the natural host Clostridium have been engineered, butanol titers in the anaerobe seem to be limited by its intolerance to butanol less than 13 ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-017-2246-1
更新日期：2017-04-01 00:00:00
abstract：:A mutant of Bacillus subtilis AF 1 (an antifungal strain with plant growth-promoting activity, resistant to 100 μg streptomycin/ml) was isolated from pigeon pea rhizosphere and, when used in peanut bacterization, decreased fungal and bacterial numbers and increased the actinomycete population in the rhizosphere. Over ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00327964
更新日期：1994-11-01 00:00:00
abstract：:Quaternary ammonium salts (QASs) are ubiquitous in nature, being found in organisms ranging from microorganisms to vertebrates (e.g., glycine betaine, carnitine) where they have important cellular functions. QASs are also obtained by chemical synthesis. These compounds, due to their diverse chemical structure (e.g. mo...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s11274-020-02978-0
更新日期：2021-01-11 00:00:00
