当前位置： SCI文献检索 > WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY期刊下所有文献 > Real-time loop-mediated isothermal amplification assay for rapid and sensitive detection of anthrax spores in spiked soil and talcum powder.

Real-time loop-mediated isothermal amplification assay for rapid and sensitive detection of anthrax spores in spiked soil and talcum powder.

Abstract：

:Loop-mediated isothermal amplification (LAMP) assay is a powerful and innovative gene amplification technique that specifically amplifies the target gene under isothermal conditions with a high degree of sensitivity, rapidity and specificity. The major advantage of the LAMP assay is monitoring of amplified products without the requirement of any sophisticated equipment. In the present study a real time LAMP assay was employed for rapid and real time detection of Bacillus anthracis spores spiked in 0.1 g of soil and talcum powder ranging from 2 to 10(7) spores. DNA was isolated from spiked soil and talcum powder using PBS containing 1% Triton X-100, and heat treatment. Isolated DNA was used as template for LAMP and PCR. LAMP amplification was obtained in 60 min under isothermal condition at 63°C by employing a set of six primers targeting the pag gene of B. anthracis. The detection limit of LAMP assay in soil and talcum powder was found to be as low as 5 spores, compared to 10(3) spores and 10(4) spores by PCR in talcum powder and soil, respectively. The findings suggest that LAMP is a more rapid and sensitive assay than PCR for detecting anthrax spores, additionally the methodology to prepare DNA from spiked samples is simple, rapid and cost effective.

journal_name

World J Microbiol Biotechnol

journal_title

World journal of microbiology & biotechnology

authors

Jain N,Kumar JS,Parida MM,Merwyn S,Rai GP,Agarwal GS

doi

10.1007/s11274-010-0592-3

subject

Has Abstract

pub_date

2011-06-01 00:00:00

pages

1407-13

issue

6

eissn

0959-3993

issn

1573-0972

journal_volume

27

pub_type

杂志文章

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