Abstract：
:Atmospheric and room temperature plasma (ARTP) was first employed to generate mutants of Actinomyces JN537 for improving acarbose production. To obtain higher acarbose producing strains, the method of screening the strains for susceptibility to penicillin was used after treatment with ARTP. The rationale for the strategy was that mutants showing penicillin susceptibility were likely to be high acarbose producers, as their ability to synthesize cell walls was weak which might enhance metabolic flux to the pathway of acarbose biosynthesis. Acarbose yield of the mutant strain M37 increased by 62.5 % than that of the original strain. The contents of monosaccharides and amino acids of the cell wall of M37 were lower than that of the original strain. The acarbose production ability in mutant strain remained relatively stable after 10 generations. This work provides a promising strategy for obtaining high acarbose-yield strains by combination of ARTP mutation method and efficient screening technique.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Ren F,Chen L,Tong Qdoi
10.1007/s11274-016-2156-7subject
Has Abstractpub_date
2017-01-01 00:00:00pages
16issue
1eissn
0959-3993issn
1573-0972pii
10.1007/s11274-016-2156-7journal_volume
33pub_type
杂志文章abstract：:Mercury-resistance genes were detected byin situ hybridization using new synthetic oligonucleotide probes specific formerA andmerB genes according to the published sequences of the corresponding enzymes. These DNA probes were used for the detection of specific mercury-resistant microorganisms isolated from the Rhine R...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01200680
更新日期：1992-01-01 00:00:00
abstract：:Cellulolytic fungi, 34 strains, were isolated from samples taken from palm oil mill residues and effluent, and high cellulase producers selected in comparison with nine known reference strains. Although 13 isolates showed good filter paper distintegration within 14 days, only eight isolates exhibited clearing zones ar...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01238799
更新日期：1992-11-01 00:00:00
abstract：:Basidiocarp of Agaricus blazei (=Agaricus brasiliensis; =Agaricus subrufescens) is used as teas or capsules due to its antineoplastic effect but there are few reports of using mycelium for this purpose. The objective of this study was to evaluate the antineoplastic activity on sarcoma 180 cells implanted in mice of tw...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-014-1656-6
更新日期：2014-08-01 00:00:00
abstract：:Pseudomonas strains were isolated from the rhizosphere of maize grown in yellow-red latosol from Rio de Janeiro, Brazil, to serve as a delivery system for heterologous genes and for risk assessment studies in tropical soils. Selected strains were modified by insertion of the cryIVB gene from Bacillus thuringiensis and...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00327720
更新日期：1996-11-01 00:00:00
abstract：:Unsheared DNA has been isolated from Rhodotorula and Rhodosporidium yeasts using a cell-wall-digesting enzyme preparation from Paecilomyces lilacinus. Pulsed-field gel electrophoresis indicated that at least 11 chromosomes were present in Rhodot. gracilis ATCC 90950. The DNA was amenable to digestion with restriction ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00367101
更新日期：1995-05-01 00:00:00
abstract：:Glutathione (GSH) and S-adenosyl methionine (SAM) have been applied as liver-protective factors to prevent and treat many different liver damages and diseases. Due to their low stability and short half-life, oral administration of GSH or SAM might be replaced by continuous supplying through living lactic bacteria in y...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-019-2759-x
更新日期：2019-11-14 00:00:00
abstract：:An important and useful bamboo species, fishscale bamboo (Phyllostachys heteroclada Oliver), is broadly distributed in Southeast China and has multiple purposes, including uses in cuisine, weaving, Chinese medicine and ecological protection. However, no previous studies have focused on the endophytes of this plant. In...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-017-2267-9
更新日期：2017-06-01 00:00:00
abstract：:The competitiveness of fourRhizobium sp. strains infectingAcacia senegal and originating in the Sudan was assessed in a growth chamber experiment using Sudanese soil, WhenAcacia senegal was inoculated with pure cultures of the strains, there were statistically significant differences among the strains with respect to ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01195848
更新日期：1992-03-01 00:00:00
abstract：:Huperzia serrata has many important medicinal properties with proven pharmacological potential. Some of these properties may be mediated by its endophytic fungi. To test this hypothesis, in the present study, we provided a first insights into evaluating the species composition and acetylcholinesterase (AChE) inhibitor...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-015-1966-3
更新日期：2016-02-01 00:00:00
abstract：:The new transcription factor Sge1 has garnered much attention in filamentous fungi recently, which highlights its role in pathogenicity, conidiation, and the production of secondary metabolites. In this study, we demonstrated that FgSge1 is localized in the nucleus in Fusarium graminearum using fluorescent protein GFP...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-015-1894-2
更新日期：2015-09-01 00:00:00
abstract：:This study was conducted to evaluate the effects of Bacillus subtilis SC02 supplement on water quality, microbial community diversity and structure in a grass carp (Ctenopharyngodon idellus) culture. Our selected strain, B. subtilis SC02, significantly reduced ammonia, nitrite and total nitrogen levels in water over a...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1327-z
更新日期：2013-09-01 00:00:00
abstract：:A nonautonomous element of 624 bp, called RetroCl1 (Retroelement Colletotrichum lindemuthianum 1), was identified in the plant pathogenic fungus Colletotrichum lindemuthianum. RetroCl1 contains terminal direct repeats (223 bp) that are surrounded by CTAGT sequences. It has a short internal domain of 178 bp and shows c...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-011-0909-x
更新日期：2012-03-01 00:00:00
abstract：:The incidence of fluoroquinolone-resistant Shigella strains has risen rapidly, presumably in response to ciprofloxacin antibiotic stress. Understanding the molecular mechanisms underlying this resistance phenotype is critical to developing novel and effective therapeutic strategies. In this study, the frequency of cip...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-012-1190-3
更新日期：2013-02-01 00:00:00
abstract：:Cyanobacterial 16S ribosomal RNA gene diversity was examined in a benthic mat on Fildes Peninsula of King George Island (62º09'54.4''S, 58º57'20.9''W), maritime Antarctica. Environmental DNA was isolated from the mat, a clone library of PCR-amplified 16S rRNA gene fragments was prepared, and amplified ribosomal DNA re...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-010-0578-1
更新日期：2011-06-01 00:00:00
abstract：:The bioleachings of chalcopyrite ore were compared after inoculating different cultures enriched from the original acid mine drainage sample. The results showed that the higher bioleaching performance was achieved for inoculation with the enrichment D (0.5 % S, 2 % iron and 1 % chalcopyrite) compared to other enrichme...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-012-1179-y
更新日期：2013-02-01 00:00:00
abstract：:Microorganisms associated with fermentation ofmasa wereLactobacillus plantarum, Pediococcus acidilactici, Lactobacillus fermentum andSaccharomyces cerevisiae. During the fermentation of maize grains, the temperature and moisture increased and the pH decreased. Most amino acids decreased in concentration during ferment...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01200938
更新日期：1990-06-01 00:00:00
abstract：:Progesterone was completely hydroxylated, principally to 11β,14α dihydroxyprogesterone by Cunninghamella blakesleeana NCIM 687 in 48h. ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00367121
更新日期：1995-05-01 00:00:00
abstract：:Iron-oxidizing bacteria (FeOB) refers to a group of bacteria with the ability to exchange and accumulate divalent iron dissolved in water as trivalent iron inside and outside the bacterial cell. Most FeOB belong the largest bacterial phylum, Proteobacteria. Within this phylum, FeOB with varying physiology with regards...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s11274-018-2491-y
更新日期：2018-07-04 00:00:00
abstract：:Brucellae are Gram-negative intracellular bacterial pathogens that infect humans and animals, bringing great economic burdens to developing countries. Live attenuated Brucella vaccines (strain M5-90 or others) are the most efficient means for prevention and control of animal brucellosis. However, these vaccines have s...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-016-2015-6
更新日期：2016-04-01 00:00:00
abstract：:Cobamides ('complete' corrinoids) are essential for organohalide-respiring bacteria because they act as cofactors of reductive dehalogenases (RDases). RDases are the key enzymes in organohalide respiration, a process relevant for environmental remediation. More than a decade ago, the unusual norpseudo-B12 was identifi...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s11274-017-2258-x
更新日期：2017-05-01 00:00:00
abstract：:Mangrove fungi are vastly unexplored for enzymes with industrial application. This study aimed to assess the biocatalytic activity of mangrove fungal xylanases on recycled paper pulp. Forty-four mangrove fungal (MF) isolates were initially screened for xylanolytic activity in minimal medium with corn cob xylan as the ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-012-1220-1
更新日期：2013-04-01 00:00:00
abstract：:We characterized the culturable, heterotrophic bacterial community in soil collected from a former alpine military site contaminated with petroleum hydrocarbons. The physiologically active eubacterial community, as revealed by fluorescence-in situ-hybridization, accounted for 14.9 % of the total (DAPI-stained) bacteri...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1594-8
更新日期：2014-06-01 00:00:00
abstract：:Trichoderma asperellum parasitizes a large variety of phytopathogenic fungi. The mycoparasitic activity of T. asperellum depends on the secretion of complex mixtures of hydrolytic enzymes able to degrade the host cell wall and proteases which are a group of enzymes capable of degrading proteins from host. In this stud...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1373-6
更新日期：2013-11-01 00:00:00
abstract：:Nine inter-generic somatic hybrids named as pfle were produced through PEG-mediated protoplast fusion between Pleurotus florida and Lentinula edodes using double selection method. Hybridity of the newly developed strains was established on the basis of colony morphology, mycelial growth, hyphal traits, fruit-body prod...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-014-1652-x
更新日期：2014-08-01 00:00:00
abstract：:Anaerobic digestion (AD) is the most popular path of organic waste disposal. It is often used in wastewater treatment plants for excessive sludge removal. Methanogenic fermentation had usually been performed under mesophilic conditions, but in the past few years the thermophilic processes have become more popular due ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-014-1731-z
更新日期：2014-12-01 00:00:00
abstract：:In a survey of endophytic fungal biodiversity, an antimicrobial endophytic isolate zjwcf069 was obtained from twigs of Forsythia viridissima, Zhejiang Province, Southeast China. Zjwcf069 was then identified as Pezicula sp. through combination of morphological and phylogenetic analysis based on ITS-rDNA. Zjwcf069 here ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-014-1686-0
更新日期：2014-10-01 00:00:00
abstract：:Adhesion ability is a primary criterion for the selection of probiotic microorganisms. Lactic acid bacteria contribute the majority of microorganisms with probiotic properties. They have several important mechanisms for intestinal epithelial cell adhesion. In order to adhere to the intestinal cells, they generally use...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s11274-019-2730-x
更新日期：2019-10-01 00:00:00
abstract：:One strain of algicidal bacterium, named as S088, was isolated from the intestine of healthy sea cucumbers (Stichopus horrens) in the South China Sea. Based on the analysis of its biochemical characteristics and 16S rDNA gene sequence, S088 was identified as Bowmanella denitrificans. Importantly, the algicidal activit...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1478-y
更新日期：2014-02-01 00:00:00
abstract：:An extracellular polygalacturonase (EC 3.2.1.15) fromGeotrichum candidum ATCC 34614 grown onsauerkraut brine was produced and characterized. Polygalacturonic acid markedly increased the enzyme yield in the brine. The fungus produced the highest activity (290 U/l) in brine with 0.3% (w/v) polygalacturonic acid. The pH ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01201944
更新日期：1992-09-01 00:00:00
abstract：:When 50MM FeSO4 was added to cow dung and poultry litter waste which had been processed in daily-fed batch digesters, digesters subsequently unfed showed a faster conversion of substrate and overloaded digesters stabilized within 48 h. Early stabilization of digesters was achieved by adding 20 or 50MM FeSO4 though the...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00360890
更新日期：1994-03-01 00:00:00
