Abstract:
:Mercury-resistance genes were detected byin situ hybridization using new synthetic oligonucleotide probes specific formerA andmerB genes according to the published sequences of the corresponding enzymes. These DNA probes were used for the detection of specific mercury-resistant microorganisms isolated from the Rhine River which had been polluted 3 years previously in 1986. Mercuric reductase and organomercurial lyase genes persist in the bacterial genome even after the disappearance of the pollutant but are absent in axenic amoebae. A total of 49 bacterial isolates showed DNA homologies with the(32)P-labelled DNA probes and 15 free-living amoebae were selected due to their harboured symbiotic mercury-resistant bacteria.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Mirgain I,Hagnere C,Green GA,Harf C,Monteil Hdoi
10.1007/BF01200680subject
Has Abstractpub_date
1992-01-01 00:00:00pages
30-8issue
1eissn
0959-3993issn
1573-0972journal_volume
8pub_type
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journal_title:World journal of microbiology & biotechnology
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journal_title:World journal of microbiology & biotechnology
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journal_title:World journal of microbiology & biotechnology
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journal_title:World journal of microbiology & biotechnology
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journal_title:World journal of microbiology & biotechnology
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journal_title:World journal of microbiology & biotechnology
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journal_title:World journal of microbiology & biotechnology
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