Synthetic oligonucleotide probes for detection of mercury-resistance genes in environmental freshwater microbial communities in response to pollutants.

Abstract:

:Mercury-resistance genes were detected byin situ hybridization using new synthetic oligonucleotide probes specific formerA andmerB genes according to the published sequences of the corresponding enzymes. These DNA probes were used for the detection of specific mercury-resistant microorganisms isolated from the Rhine River which had been polluted 3 years previously in 1986. Mercuric reductase and organomercurial lyase genes persist in the bacterial genome even after the disappearance of the pollutant but are absent in axenic amoebae. A total of 49 bacterial isolates showed DNA homologies with the(32)P-labelled DNA probes and 15 free-living amoebae were selected due to their harboured symbiotic mercury-resistant bacteria.

authors

Mirgain I,Hagnere C,Green GA,Harf C,Monteil H

doi

10.1007/BF01200680

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

30-8

issue

1

eissn

0959-3993

issn

1573-0972

journal_volume

8

pub_type

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