Immunocytochemistry controls using cell culture.

Abstract:

:A continuing problem in cytology laboratories is the lack of adequate control material for immunocytochemical testing. Usual control procedures involve testing paraffin-embedded control materials along with the patient specimens. These control materials are fundamentally unlike cytologic preparations. We have developed a method to make control preparations for immunocytochemical analysis using cultured anaplastic cells with known antigenic features from commercial sources. Cell lines included melanoma, rhabdomyosarcoma, T-cell leukemia, and squamous-cell carcinoma. Modified Saccomano and acetone fixation coupled with the cytospin technique enabled good-quality preparations. Cell lines were tested with antibodies for HMB-45, actin, leukocyte common antigen (LCA) and cytokeratin, which avidin-biotin immunoperoxidase and diaminobenzidine (DAB) as chromogens. Our final preparations were easily interpretable with excellent morphologic preservation of cellular detail. Cultured cells provide a superior method for preparing almost unlimited numbers of control slides for immunocytochemistry for laboratories with access to a tissue culture facility.

journal_name

Diagn Cytopathol

journal_title

Diagnostic cytopathology

authors

Kurtycz DF,Logrono R,Leopando M,Slattery A,Inhorn SL

doi

10.1002/(sici)1097-0339(199707)17:1<74::aid-dc17>3

subject

Has Abstract

pub_date

1997-07-01 00:00:00

pages

74-9

issue

1

eissn

8755-1039

issn

1097-0339

pii

10.1002/(SICI)1097-0339(199707)17:1<74::AID-DC17>3

journal_volume

17

pub_type

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