Abstract:
:A continuing problem in cytology laboratories is the lack of adequate control material for immunocytochemical testing. Usual control procedures involve testing paraffin-embedded control materials along with the patient specimens. These control materials are fundamentally unlike cytologic preparations. We have developed a method to make control preparations for immunocytochemical analysis using cultured anaplastic cells with known antigenic features from commercial sources. Cell lines included melanoma, rhabdomyosarcoma, T-cell leukemia, and squamous-cell carcinoma. Modified Saccomano and acetone fixation coupled with the cytospin technique enabled good-quality preparations. Cell lines were tested with antibodies for HMB-45, actin, leukocyte common antigen (LCA) and cytokeratin, which avidin-biotin immunoperoxidase and diaminobenzidine (DAB) as chromogens. Our final preparations were easily interpretable with excellent morphologic preservation of cellular detail. Cultured cells provide a superior method for preparing almost unlimited numbers of control slides for immunocytochemistry for laboratories with access to a tissue culture facility.
journal_name
Diagn Cytopatholjournal_title
Diagnostic cytopathologyauthors
Kurtycz DF,Logrono R,Leopando M,Slattery A,Inhorn SLdoi
10.1002/(sici)1097-0339(199707)17:1<74::aid-dc17>3subject
Has Abstractpub_date
1997-07-01 00:00:00pages
74-9issue
1eissn
8755-1039issn
1097-0339pii
10.1002/(SICI)1097-0339(199707)17:1<74::AID-DC17>3journal_volume
17pub_type
杂志文章abstract::Sclerosing polycystic adenosis (SPA) is a recently described, rare lesion of the salivary gland analogous to fibrocystic disease of the breast. Recognition of this benign entity is important since the differential diagnosis includes other more common benign and malignant salivary gland neoplasms, particularly mucoepid...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20671
更新日期:2007-07-01 00:00:00
abstract::Necrosis is a worrisome cytologic feature in breast aspirates which, when paired with suspicious clinical and mammographic findings, could potentially result in a false positive diagnosis. A 66-yr-old female presented with clinical evidence of stage 1 breast carcinoma supported by the mammographic and ultrasound findi...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840110216
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:The difference in expression of sputum cells types between the two main types of chronic obstructive pulmonary disease (COPD) is largely unknown. This study aims to investigate the difference and then wants to depend on sputum cells sorting to direct the treatment of COPD. METHODS:A total of 218 patients wi...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23818
更新日期:2017-12-01 00:00:00
abstract::We report a case of nodal nevus present in enlarged lymph nodes with changes of dermatopathic lymphadenopathy sampled by fine-needle aspiration (FNA) cytology prior to clinical evaluation of the patient. This lymph node pathology was established later by lymph node excisional biopsy, by which along with a skin biopsy ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20101
更新日期:2004-09-01 00:00:00
abstract::Microfilariae of various nematodes, including Loa loa, Dirofilariae, and Onchocerca volvulus, have been identified in the central nervous system (CNS). The CNS, however, is a rare site for the isolation of microfilariae of Wuchereria bancrofti. To the best of our knowledge, the presence of microfilariae of W. bancroft...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.10074
更新日期:2002-03-01 00:00:00
abstract:BACKGROUND:Diagnosis of hepatoblastoma (HBL) is based on characteristic clinical and radiological presentation, young age and marked elevation of serum α-fetoprotein (aFP). Fine needle aspiration (FNA) technique is successfully used in the diagnosis of hepatoblastoma. To evaluate the value of FNA in the diagnosis and s...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23642
更新日期:2017-02-01 00:00:00
abstract::Pancreatoblastoma is a rare tumor and has been reported only four times in the cytologic literature, three times in fine-needle aspiration (FNA) biopsy and once in an imprint of resected tumor. We are reporting the fourth case of FNA cytology with immunohistochemical and electron microscopic studies. The patient is a ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/dc.20355
更新日期:2005-10-01 00:00:00
abstract::The case of a 22-yr-old male who after a brief febrile episode developed autoimmune hemolytic anemia and right pulmonary infiltrate with pleural effusion is presented. Cytologic examination of the pleural fluid revealed lymphocytosis and hemophagocytosis, primarily of red blood cells (RBCs) by mature histiocytes. Ther...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2048
更新日期:2001-10-01 00:00:00
abstract::Telecytology has been used for education, training, and consultation. Cytological studies from gynecological, nongynecological and fine-needle aspiration cytology (FNAC) specimens (including studies of thyroid FNAC) analyzed the diagnostic accuracy and reproducibility of telecytology-based predominantly on static digi...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.22958
更新日期:2013-09-01 00:00:00
abstract::The cytomorphologic findings in fine-needle aspirates from 7 cases of salivary duct carcinoma (SDC) are reviewed and correlated with the histologic features. Malignant cytologic characteristics are clear in this tumor, and no false-negative results were obtained. But the absence of cribriform or papillary groups sugge...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199808)19:2<120::aid-dc11>
更新日期:1998-08-01 00:00:00
abstract::Solitary pleural amyloid nodule is rare and radiologically can mimic neoplastic lesions. This report describes two cases of this entity diagnosed by fine-needle aspiration biopsy in patients without systemic amyloid deposits. The histologic, ultrastructural, and cytologic features are described. ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840070319
更新日期:1991-01-01 00:00:00
abstract::The objectives of this study were to identify key cytologic features for the morphologic differentiation of bronchioloalveolar carcinoma from reactive pulmonary proliferations, and to determine if morphometry of the cytologic specimens could provide additional data to distinguish the two processes. We analyzed 15 morp...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199705)16:5<396::aid-dc4>3
更新日期:1997-05-01 00:00:00
abstract::Prostate-specific antigen (PSA) is secreted by both normal and neoplastic acinar cells of the prostate gland, and the immunohistochemical detection of PSA is widely accepted as an excellent method for confirming the prostatic origin of metastatic tumor implants in men with prostate cancer. Less recognized is the obser...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199809)19:3<221::aid-dc14>
更新日期:1998-09-01 00:00:00
abstract::To avoid contamination of equipment and reduce risks of infection, intraoperative cytology (IOC) is a useful substitute to conventional frozen section in the diagnosis of infectious diseases. One of the various histomorphologic patterns of infections is the granuloma, which sometimes may be difficult to diagnose cytol...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199801)18:1<62::aid-dc10>3
更新日期:1998-01-01 00:00:00
abstract::Breast disease is of great concern to patients and health care providers alike. Malignancies of the breast and their various presentations can pose tremendous challenges with respect to early diagnosis and effective treatment. Carcinoma of the breast may appear in several forms and produce one or more ectopic substanc...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/dc.20485
更新日期:2006-10-01 00:00:00
abstract::To determine if fine needle aspiration (FNAB) of mycobacterial lymphadenopathy can differentiate infection with M. bovis BCG (BCG) from M. tuberculosis (TB) and whether HIV status affects discriminatory cytological features. A retrospective study of culture positive, fine needle aspiration biopsies of lymph nodes in c...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21378
更新日期:2011-04-01 00:00:00
abstract::Endoscopic ultrasound-guided fine-needle aspiration biopsy (EUS-FNAB) has become a widely used method of pre-operatively evaluating pancreatic masses. This technique introduces gastrointestinal contamination into the specimen, which poses a diagnostic pitfall. The cytomorphologic features of these contaminants have no...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20343
更新日期:2005-12-01 00:00:00
abstract::Many reports have been published on the accuracy of the cervical vaginal smear for squamous lesions, and the literature contains fewer reports on the accuracy of the cervical vaginal smear for glandular lesions. The sensitivity of glandular lesion diagnosis depends on the subtype of lesion. The diagnostic sensitivity ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/1097-0339(200008)23:2<127::aid-dc13>3.0.co
更新日期:2000-08-01 00:00:00
abstract::Metaplastic cells with nebular cytoplasmic changes in the cervical smear are classified in the Dutch coding system for cervical screening as KOPAC O8 cells. Since these nebulated cells are already documented by Papanicolaou, we refer to these cells as Papanicolaou's nebular cells. We examined the simultaneous presence...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21523
更新日期:2012-03-01 00:00:00
abstract:OBJECTIVES:According to the American Society of Colposcopy and Cervical Pathology (ASCCP), cervical cytology patients who test positive for atypical squamous cells of undetermined significance (ASCUS) and the human papilloma virus (HPV) positive are recommended to undergo colposcopies. This study compared the colposcop...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24029
更新日期:2019-02-01 00:00:00
abstract::The 2001 Bethesda System diagnostic category of atypical squamous cells cannot exclude a high grade squamous intraepithelial lesion (ASC-H) was developed and implemented after many studies that proved its clinical relevance. In this study, we describe the practice experience of a hospital-based reference laboratory wi...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20227
更新日期:2005-04-01 00:00:00
abstract::A 25-yr-old male presented with a cerebellar mass, underwent a suboccipital craniotomy, and was diagnosed with medulloblastoma. Six months later he developed a large mass in the right iliac crest. Fine-needle aspiration biopsy (FNAB) confirmed the diagnosis of metastatic medulloblastoma. The diagnosis of metastatic me...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.1078
更新日期:2001-05-01 00:00:00
abstract::Since calcific deposits in soft tissue may clinically resemble a tumor, it is feasible to investigate them by fine-needle aspiration (FNA) cytology. In this presentation we describe a case in the breast with extensive accumulation of acellular liquid calcific material which was sampled by the noninvasive technique of ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(200001)22:1<30::aid-dc9>3.
更新日期:2000-01-01 00:00:00
abstract::Differential leukocyte counts on noninflammatory synovial fluids (NISF) are not widely reported or used in research, apparently due to technical difficulties related to either high viscosity or low numbers of cells. We describe an evaluation of a technique using hyaluronidase and cytospin preparations to study NISF. T...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(200004)22:4<256::aid-dc13>
更新日期:2000-04-01 00:00:00
abstract::We used various meta-analytic methods to compare 25 studies describing fine-needle aspiration (FNA) cytologic analyses performed from 1984 to 2007 on palpable breast masses. We found that in the 25 studies examined, the sensitivity ranged from 78% to 100%, the specificity ranged from 76% to 100%, and the diagnostic od...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,meta分析
doi:10.1002/dc.20809
更新日期:2008-05-01 00:00:00
abstract::Most of the available data in the literature describe the cytomorphological features of exfoliated malignant epithelial cells in urine. There are no established diagnostic features that characterize the morphology of exfoliated malignant mesenchymal tumor in urine. Here we highlight the problems in the diagnosis of th...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20053
更新日期:2004-10-01 00:00:00
abstract::It is commonly believed that cytodiagnosis of Hodgkin's lymphoma (HL) is much easier than that of non-Hodgkin lymphoma (NHL). However, recognition of certain NHL subtypes with Reed-Sternberg (R-S)-like cells and results of immunohistochemical studies point to the contrary. To study the limitations of cytology in diagn...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21064
更新日期:2009-08-01 00:00:00
abstract::Cytological evaluation of cervical smears has been carried out in 4,338 women attending the Gynecology and Obstetrics Department and Family Planning Clinic of Queen Mary's Hospital, Lucknow, India, since April 1971. The incidence of cervical dysplasia was found to be 4.6% (205 out of 4,338) in the series, while malign...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840030107
更新日期:1987-03-01 00:00:00
abstract::A case of granulocyte colony-stimulating factor (G-CSF)-producing small-cell carcinoma of the uterine cervix is described. In a 70-yr-old woman, clusters of small cells with hyperchromatic nuclei at a high nuclear/cytoplasmic ratio were detected cytologically in the cervix. These clusters were diagnosed as a small-cel...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/1097-0339(200010)23:4<269::aid-dc11>3.0.co
更新日期:2000-10-01 00:00:00
abstract:BACKGROUND:We previously found that the presence of atypical urothelial tissue fragments (AUTF) was associated with an increased risk of high-grade urothelial carcinoma (HGUC) but not low-grade urothelial neoplasia (LGUN) in voided urine (VU) specimens. However, we subsequently found that patients with LGUN were more l...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23767
更新日期:2017-09-01 00:00:00