Crystal structure of GTPase-activating domain from human MgcRacGAP.

abstract：:Lafora disease, a progressive myoclonus epilepsy, is an autosomal recessive disease caused in approximately 80% of cases by mutation of the EPM2A gene, which encodes a dual specificity protein phosphatase called laforin. In addition to its phosphatase domain, laforin contains an N-terminal carbohydrate-binding domain ...

journal_title：Biochemical and biophysical research communications

authors： Wang W,Roach PJ

更新日期：2004-12-17 00:00:00

abstract：:Cancer chemotherapy with taxol often fails due to acquired resistance of cancer cells, which is frequently associated with an overexpression of P-gp and alterations of beta-tubulin. A taxol-resistant cell line, QGY-TR50, derived from a human hepatocellular carcinoma (HCC) QGY-7703 cell line was used to investigate the...

journal_title：Biochemical and biophysical research communications

authors： Zhou J,Cheng SC,Luo D,Xie Y

更新日期：2001-02-09 00:00:00

abstract：:A L-delta 1-pyrroline-5-carboxylate reductase activity has been detected in crude extracts of Desulfovibrio desulfuricans Norway. This P5C reductase activity is also found when a 2.5 kb D. desulfuricans DNA fragment is introduced into an Escherichia coli proC mutant. Although it restores growth of the proC mutant, the...

journal_title：Biochemical and biophysical research communications

authors： Fons M,Cami B,Chippaux M

更新日期：1991-09-16 00:00:00

abstract：:Human platelets contained proteins which cross-reacted with antisera specific for brain protein kinase C-alpha and -beta. When platelets were incubated with 12-O-tetradecanoylphorbol-13-acetate there was a rapid accumulation of protein kinase C-alpha in the particulate fraction associated with a loss of this subspecie...

journal_title：Biochemical and biophysical research communications

authors： Fournier A,Hardy SJ,Clark KJ,Murray AW

更新日期：1989-06-15 00:00:00

abstract：:Nonsteroidal anti-inflammatory drugs (NSAIDs) have a preventive effect against colorectal cancer. Although inhibition of cyclooxygenase-2 plays a crucial role in the suppression of tumors, precise mechanisms of their action remain to be disclosed. To identify genes involved in the growth-suppressive effect of NSAIDs, ...

journal_title：Biochemical and biophysical research communications

authors： Iizaka M,Furukawa Y,Tsunoda T,Akashi H,Ogawa M,Nakamura Y

更新日期：2002-03-29 00:00:00

abstract：:Direct chemical acetylation of an oligonucleosomal template for bacteriophage T7 RNA polymerase is accompanied by a substantial increase in its capability to support RNA synthesis. The template was assembled from a plasmid, containing a promoter and a terminator for T7 RNA polymerase, plus one (H3-H4)2 tetramer and tw...

journal_title：Biochemical and biophysical research communications

authors： Hernández F,Puerta C,López-Alarcón L,Palacián E

更新日期：1995-08-04 00:00:00

abstract：:Groucho is a corepressor that forms a macromolecular complex for its corepressor activity, in which HDAC1 is an essential component for the modulation of chromatin structure and transcriptional repression of target genes. Here, we show that Groucho is covalently conjugated with small ubiquitin-related modifier-1 (SUMO...

journal_title：Biochemical and biophysical research communications

authors： Ahn JW,Lee YA,Ahn JH,Choi CY

更新日期：2009-01-30 00:00:00

abstract：:Strong temperature dependent low angle X-ray scattering by trifluoroethanol- and hexafluoro-2-propanol-water mixtures was observed in conditions commonly used in NMR work on peptides. At least two types of molecular effects may contribute to the observed scattering: formation of clathrate hydrate-like aggregates of al...

journal_title：Biochemical and biophysical research communications

authors： Kuprin S,Gräslund A,Ehrenberg A,Koch MH

更新日期：1995-12-26 00:00:00

abstract：:Cultured human endothelial cells produce increased levels of PA-I when stimulated with IL-1. The stimulatory effect was elicited by both alpha (pI 5) and beta (pI 7) IL-1 molecules. The effect was both dose and time-dependent, plateau reached after 8 h. The PA-I measured in cell extracts was SDS-resistant with an appa...

journal_title：Biochemical and biophysical research communications

authors： Grames M,Breviario F,Pintucci G,Millet I,Dejana E,van Damme J,Donati MB,Mussoni L

更新日期：1986-09-14 00:00:00

abstract：:An in organello footprinting approach has been used to probe a protein-DNA interaction of a nuclear coded 25 kDa protein, previously isolated in our laboratory, that binds "in vitro" a region within the ND2 gene, located upstream of the Ori-L. Footprinting studies with the purine-modifying reagent dimethyl sulfate and...

journal_title：Biochemical and biophysical research communications

authors： Cingolani G,Capaccio L,D'Elia D,Gadaleta G

更新日期：1997-02-24 00:00:00

abstract：:The marked impairment of hepatic drug metabolism during inflammation and infections has been known for many years and shown to result from down-regulation of cytochrome P450s (CYP) by cytokines. However, the mechanism of this repression is unknown. Using primary cultures of human hepatocytes, we show here that interle...

journal_title：Biochemical and biophysical research communications

authors： Pascussi JM,Gerbal-Chaloin S,Pichard-Garcia L,Daujat M,Fabre JM,Maurel P,Vilarem MJ

更新日期：2000-08-11 00:00:00

abstract：:To identify the source of arachidonic acid utilized for eicosanoid production, rabbit alveolar macrophages were challenged with 1.0 microM 12-O-tetradecanoylphorbol-13-acetate (TPA) or 3.8 microM Ca+2 ionophore A23187 for 3 h. Upon stimulation with TPA, a loss of [3H]arachidonic acid from phosphatidylcholine, phosphat...

journal_title：Biochemical and biophysical research communications

authors： Cochran FR,Connor JR,Roddick VL,Waite BM

更新日期：1985-07-31 00:00:00

abstract：:The octaheme cytochrome c3 (Mr 26000) from Desulfovibrio desulfuricans Norway was studied using cyclic voltammetry at the pyrolytic graphite electrode. The kinetics of reduction of the octaheme cytochrome c3 (Mr 26000) from D. desulfuricans Norway by the Ni-Fe-Se hydrogenase purified from the same organism was investi...

journal_title：Biochemical and biophysical research communications

authors： Haladjian J,Bianco P,Guerlesquin F,Bruschi M

更新日期：1991-08-30 00:00:00

abstract：:Western immunoblots of whole cell lysate and crude membrane extract of an in vitro selected sodium m-arsenite-resistant L. donovani strain revealed a 230-kDa protein identified by an anti-P-glycoprotein (Pgp) antibody. Immunofluorescence microscopy, using the same antibody, detected putative Pgp on resistant parasites...

journal_title：Biochemical and biophysical research communications

authors： Kaur J,Dey CS

更新日期：2000-05-19 00:00:00

abstract：:Hedgehog (Hh) signaling plays key roles in animal development and tissue homeostasis. Binding of the secreted ligand to its Ptch1 receptor triggers Hh signaling through distinct canonical or noncanonical signaling pathways. Canonical Hh signaling leads to the activation of Gli transcription factors to induce Hh target...

journal_title：Biochemical and biophysical research communications

authors： Lee H,Ko HW

更新日期：2016-11-25 00:00:00

abstract：:Calumenin is a multiple EF-hand Ca2+-binding protein located in endo/sarcoplasmic reticulum of mammalian tissues. In the present study, we cloned two rabbit calumenin isoforms (rabbit calumenin-1 and -2, GenBank Accession Nos. SY225335 and AY225336, respectively) by RT-PCR. Both isoforms contain a 19 aa N-terminal sig...

journal_title：Biochemical and biophysical research communications

authors： Jung DH,Mo SH,Kim DH

更新日期：2006-04-28 00:00:00

abstract：:Adenosine inhibits ram sperm adenylate cyclase activity which is membrane-bound and comprises only the catalytic subunit. The inhibition parameters of adenylate cyclase by adenosine were not modified when the enzyme was purified 3 to 5,000 fold. Optimal inhibition by adenosine was found to require a high concentration...

journal_title：Biochemical and biophysical research communications

authors： Henry D,Ferino F,Tomova S,Ferry N,Stengel D,Hanoune J

更新日期：1986-06-30 00:00:00

abstract：:The (1)H NMR spectrum of the perchloric acid extract of carp seminal plasma was heavily congested. It is demonstrated that proton-detected C,H chemical shift correlation spectroscopy (HSQC, HSQC-TOCSY) allows an unequivocal identification of proline, glutamate, taurine, and methionine sulfoxide, although several key p...

journal_title：Biochemical and biophysical research communications

authors： Kalic M,Lauterwein J,Büsselmann G,Kamp G

更新日期：2000-03-05 00:00:00

abstract：:Lipoprotein lipase (LPL) bound to vascular endothelial cells hydrolyses triglycerides in plasma lipoproteins. To explore the role of LPL in atherogenesis, the effect of LPL-mediated lipolysis of very low density lipoproteins (VLDL) on monocyte adhesion to endothelial cells was examined. Adhesion of U937 monocytes to p...

journal_title：Biochemical and biophysical research communications

authors： Saxena U,Kulkarni NM,Ferguson E,Newton RS

更新日期：1992-12-30 00:00:00

abstract：:Ribose methylation of eukaryotic rRNA is directed by box C/D small nucleolar RNAs (snoRNAs), which pinpoint the nucleotide to be methylated in specific position within the rRNA sequence. Here, we report the identification of a novel double-guide C/D box snoRNA termed snR88 that directs methylation of two previously un...

journal_title：Biochemical and biophysical research communications

authors： Bi YZ,Qu LH,Zhou H

更新日期：2007-03-02 00:00:00

abstract：:Myocardial fibrosis is the pathological consequence of injury-induced fibroblastto-myofibroblast transition, resulting in increased stiffness and diminished cardiac function. Histone modification has been shown to play an important role in the pathogenesis of cardiac fibrosis. Here, we identified H3K27me3 demethylase ...

journal_title：Biochemical and biophysical research communications

authors： Long F,Wang Q,Yang D,Zhu M,Wang J,Zhu Y,Liu X

更新日期：2020-08-06 00:00:00

abstract：:Avian leukosis virus (ALV) is one of the main causative agent of tumor development, which brings enormous economic losses to the poultry industry worldwide. ALV can be transmitted horizontally and vertically, and the latter often give rise to more adverse pathogenicity. However, the propagation and evolution of ALV un...

journal_title：Biochemical and biophysical research communications

authors： Gao X,Li Q,Fang L,Song H,Cui Z,Meng F,Zhang Z

更新日期：2020-01-22 00:00:00

abstract：:Cystic fibrosis transmembrane conductance regulator (CFTR) plays a key role in exocrine secretion, including salivary glands. However, its functional expression in salivary glands has not been rigorously studied. In this study, we investigated the expression pattern and regulatory mechanism of CFTR in salivary glands ...

journal_title：Biochemical and biophysical research communications

authors： Shin YH,Lee SW,Kim M,Choi SY,Cong X,Yu GY,Park K

更新日期：2016-12-02 00:00:00

abstract：:Phytoplankton and zooplankton serve as major food material for primary and secondary consumers in the marine food web. Although phytoplankton are primary targets to UV radiation damaging effects, we have demonstrated that stratospheric ozone depletion will affect zooplankton to contribute to inhibition in biomass prod...

journal_title：Biochemical and biophysical research communications

authors： Soni AK,Joshi PC

更新日期：1997-02-24 00:00:00

abstract：:Rab5 is a GTP-binding protein that is crucial for endocytic machinery functions. We previously identified L-plastin as a binding protein for Rab5, using an affinity column with constitutively active Rab5. L- and T-plastin are isoforms of a plastin protein family belonging to actin-bundling proteins that are implicated...

journal_title：Biochemical and biophysical research communications

authors： Hagiwara M,Shinomiya H,Kashihara M,Kobayashi K,Tadokoro T,Yamamoto Y

更新日期：2011-04-15 00:00:00

abstract：:The purpose of this study was to elucidate the involvement of H2O2 production in cisplatin-induced nephrotoxicity. The S3 cells were more sensitive than OMCT cells in cell viability and H2O2 production during cisplatin treatment. The cytotoxicity and H2O2 production induced by cisplatin were terminated by treatment wi...

journal_title：Biochemical and biophysical research communications

authors： Tsutsumishita Y,Onda T,Okada K,Takeda M,Endou H,Futaki S,Niwa M

更新日期：1998-01-14 00:00:00

abstract：:The chemopreventive effect of nitric oxide-releasing aspirin (NO-ASA) against gastrointestinal tumorigenesis was evaluated in Min (APC(Min/+)) mice. NO-ASA consists of a traditional ASA that bears covalently attached to it an NO-releasing moiety. Four groups (N=10) of six-week-old female C57BL/6J APC(Min/+) and the co...

journal_title：Biochemical and biophysical research communications

authors： Williams JL,Kashfi K,Ouyang N,del Soldato P,Kopelovich L,Rigas B

更新日期：2004-01-16 00:00:00

abstract：:Using a cDNA glass microarray, the expression of 1081 genes in immature and mature dendritic cells (DCs) of two different individuals has been studied. The upregulation of mRNA transcripts of genes encoding the transcription factor ZFM1, Mos proto-oncogene serine/threonine-protein kinase, B-cell-specific transcription...

journal_title：Biochemical and biophysical research communications

authors： Lapteva N,Nieda M,Ando Y,Ide K,Hatta-Ohashi Y,Dymshits G,Ishikawa Y,Juji T,Tokunaga K

更新日期：2001-07-27 00:00:00

abstract：:Although the extravesicular p40 domain of the transmembrane protein, p65 synaptotagmin (Syt) 1, is essential for the non-classical export of the signal peptide-less structure, FGF1, it was not possible to identify a specific intracellular protease responsible for the processing of p65 Syt1. Surprisingly, analysis of t...

journal_title：Biochemical and biophysical research communications

authors： Bagalá C,Kolev V,Mandinova A,Soldi R,Mouta C,Graziani I,Prudovsky I,Maciag T

更新日期：2003-10-31 00:00:00

abstract：:Using minimum energy calculations and molecular dynamics techniques the preferred conformational states of LHRH and its analogues have been reported to involve a modified beta-bend between residues 5 to 8. Based on some of these models cyclic peptide analogues of LHRH antagonists were synthesised using solid phase pep...

journal_title：Biochemical and biophysical research communications

authors： Dutta AS,Gormley JJ,McLachlan PF,Woodburn JR

更新日期：1989-03-31 00:00:00