Abstract:
:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is known to interact with different biomolecules and was implicated in many novel cellular activities including programmed cell death, nuclear RNA transport unrelated to the commonly known carbohydrate metabolism. We reported here the purification of GAPDH from Chironomidae larvae (Insecta, Diptera) that showed different biologic activity towards heavy metals. It was inhibited by copper, cobalt nickel, iron and lead but was activated by zinc. The GAPDH was purified by ammonium sulphate fractionation and Chelating Sepharose CL-6B chromatography followed by Blue Sepharose CL-6B chromatography. The 150-kDa tetrameric GAPDH showed optimal activity at pH 8.5 and 37°C. The multiple alignment of sequence of the Chironomidae GAPDH with other known species showed 78 - 88% identity to the conserved regions of the GADPH. Bioinformatic analysis unveils substantial N-terminal sequence similarity of GAPDH of Chironomidae larvae to mammalian GADPHs. However, the GADPH of Chironomidae larvae showed different biologic activities and cytotoxicity towards heavy metals. The GAPDH enzyme would undergo adaptive molecular changes through binding at the active site leading to higher tolerance to heavy metals.
journal_name
Protein Pept Lettjournal_title
Protein and peptide lettersauthors
Chong IK,Ho WSdoi
10.2174/0929866511320090002subject
Has Abstractpub_date
2013-09-01 00:00:00pages
970-6issue
9eissn
0929-8665issn
1875-5305pii
PPL-EPUB-20130422-1journal_volume
20pub_type
杂志文章abstract:BACKGROUND:Cathepsin D is a lysosomal enzyme that is found in all organisms acting in protein turnover, in humans it is present in some types of carcinomas, and it has a high activity in Parkinson's disease and a low activity in Alzheimer disease. In marine organisms, most of the research has been limited to corroborat...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866526666190405124353
更新日期:2019-01-01 00:00:00
abstract::Aspergillus sp. phytase contains five disulfide bonds. In order to elucidate their role, the reactivation and refolding of phytase in the absence and presence of dithiothreitol (DTT) was investigated. The results indicated that the disulfide bonds play an important role in the catalytic activity and conformational sta...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866054395752
更新日期:2005-08-01 00:00:00
abstract:BACKGROUND:Reversibly glycosylated polypeptide (RGP), a kind of hydrosoluble and plasmodesmal-associated protein found in plants, plays a crucial role in the development of pollen. OBJECTIVE:A novel RGP 2 was isolated and identified from rape (Brassica napus L.) bee pollen. METHOD:RGP2 was isolated and purified by io...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866527666201103161302
更新日期:2020-11-03 00:00:00
abstract::Background. Obesity is a major health problem in both developed and emerging countries. Obesity is a complex disease whose etiology involves genetic factors in strong interplay with environmental determinants and lifestyle. The discovery of genetic factors and biological pathways underlying human obesity is hampered b...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/09298665113209990053
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Uterine Leiomyoma is mainly widespread non-malignant tumor. Around more than 80% woman have these particular tumor among them only 30% of them are detected. Integrin-ᵦ1 is one of the up regulated biomarkers during tumorigenesis which is also associated with structural disordered. Intrinsically disordered pro...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866525666180326114325
更新日期:2018-01-01 00:00:00
abstract::Bucain, a potent neurotoxin isolated from the venom of the Malayan krait (Bungarus candidus), induces paralysis and death. Its crystal structure has been determined at 2.10 A resolution and based on the molecular topology and hydrophobicity profile is structurally classified as a three-fingered alpha-neurotoxin posses...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609789839304
更新日期:2009-01-01 00:00:00
abstract::Protein phosphatase magnesium-dependent 1, delta (PPM1D) is a member of the PPM1 (formerly PP2C) protein phosphatase family, and is induced in response to DNA damage. The overexpression of PPM1D is thought to exert oncogenic effects through the inhibition of tumor suppressor proteins. PPM1D shows high selectivity for ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608785849236
更新日期:2008-01-01 00:00:00
abstract::We used a de novo designed, beta-hairpin forming T1 peptide as a model to investigate the kinetics of peptide fibrogenesis by a combination of light scattering (LS), circular dichroism (CD), fluorescence, and atomic force microscopy (AFM). The results demonstrate that the T1 fibrogenesis undergoes a consecutive stepwi...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610790963690
更新日期:2010-04-01 00:00:00
abstract::To investigate the function of plant plasma membrane, proteins of rice plasma membrane were analyzed and the proteins changed by cold stress were identified. Plasma membrane proteins were purified with an aqueous two-phase partitioning method from root of rice seedlings, and activity of specific H(+)-ATPase localized ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609788490140
更新日期:2009-01-01 00:00:00
abstract::We present the results of a study on the influence of albumin and prolactin concentrations and intravascular fluid pH on vein permeability for albumin. Permeability conditions were simulated depending on albumin concentration, pH value and prolactin concentration. In research model an in vitro method was applied using...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/09298665113209990082
更新日期:2014-01-01 00:00:00
abstract::Spermadhesins are a family of secretory proteins expressed in the male genital tract of pig, horse and bull. Their function and structure have been widely studied, especially those isolated from boar. However, there are no data concerning spermadhesins isolated from buck. Buck seminal plasma was collected and subjecte...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866023408670
更新日期:2002-08-01 00:00:00
abstract::VML is a lectin from Vatairea macrocarpa seeds that has various biological activities. Here, we describe three new lectin isoforms from V. macrocarpa identified through genomic DNA analysis. One of these isoforms has high similarity to VML, while another that has noteworthy differences. We have denoted the new isoform...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/09298665113209990049
更新日期:2013-11-01 00:00:00
abstract::Classification of newly determined protein structures is important in understanding their function and mechanism of action. Currently available methods employ a global structure alignment strategy and are computationally expensive. We propose a two-step methodology with a quick screen to significantly reduce the numbe...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986607781483877
更新日期:2007-01-01 00:00:00
abstract::Solid state nuclear magnetic resonance (NMR) has developed into one of the most informative and direct experimental approaches to the characterization of the molecular structures of amyloid fibrils, including those associated with Alzheimer's disease. In this article, essential aspects of solid state NMR methods are d...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/092986606775338470
更新日期:2006-01-01 00:00:00
abstract::Hop is a tetratricopeptide repeat domain (TPR)-containing co-chaperone that is able to directly associate with both Hsp70 and Hsp90. Previous data showed that the TPR2A-domain is the primary site for dimerization and that the TPR2B-domain may also play a role in dimerization. We present Hop-D456G, a mutant within the ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610790963708
更新日期:2010-04-01 00:00:00
abstract:BACKGROUND:The heterologous production of antimicrobial peptides in bacterial models can produce insoluble proteins due to the lack of proper folding. Fusion proteins have been used to increase the expression and solubility of these types of proteins with varying degrees of success. OBJECTIVES:Here, we demonstrate the...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866527666200610133407
更新日期:2020-06-10 00:00:00
abstract::Cyclophilin A (CyPA) is a cytosolic receptor of immunosuppressive drug cyclosporin A (CsA) which possesses peptidyl-prodyl cis/trans isomerase (PPIase) activity. The recombinant human CyPA (rhCyPA) gene has been expressed in E. coli M15. Purification was performed using salting-out, as well as Sephacryl S-100 and DEAE...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611797642670
更新日期:2011-12-01 00:00:00
abstract::The study of rat proteins is an indispensable task in experimental medicine and drug development. The function of a rat protein is closely related to its subcellular location. Based on the above concept, we construct the benchmark rat proteins dataset and develop a combined approach for predicting the subcellular loca...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611795222768
更新日期:2011-06-01 00:00:00
abstract::The mutation, conferring streptomycin and deoxyglucose resistance on cells, had profound effect on the kinetic and thermodynamic parameters inferring thermostabilization of beta-glucosidase from mutant 51 SM(r) of Cellulomonas biazotea. Free energy of activation for substrate binding, enthalpy and entropy of activatio...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866053587183
更新日期:2005-04-01 00:00:00
abstract::Loxosceles intermedia spider venom was subjected to proteomic analysis through a MudPIT shot-gun approach to identify the protein composition. Were identified 39 proteins which seem to responsible by the lesion of different types of tissues, to some physiopathological actions and by the prevention of structural damage...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609788923383
更新日期:2009-01-01 00:00:00
abstract::Kappa-conotoxin RIIIJ is a conopeptide to inhibit voltage-gated potassium channels, however, its detailed folding structures have yet to be studied. With the advance in computing power, it is possible to use the HP model to analyze all its possible folding structures. In this study, the amino acid sequences of kappa-c...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612800190982
更新日期:2012-05-01 00:00:00
abstract::Discriminating cell wall lytic enzymes from non lytic enzymes is a very important task for curing bacterial infections. In this paper, based on Chou's amphiphilic pseudo amino acid composition, we develop fisher-discriminant based classifier to predict cell wall lytic enzymes. Experiments show that 66.7% sensitivity w...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609787848045
更新日期:2009-01-01 00:00:00
abstract::Peptides with more than one biological activity are many a times multifunctional peptides. Two peptides with multifunctional properties from alpha (S2)-casein were stabilized in presence of cosolvents for their biological activities like ACE inhibition activity and antioxidant activity. These bioactive peptides in cos...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608784246416
更新日期:2008-01-01 00:00:00
abstract::Heparin is naturally occurring polysaccharides which interacts with seminal plasma proteins and regulate multiple steps in fertilization process. Qualitative and quantitative information regarding the affinity for heparin-seminal plasma proteins interactions is not generally well documented and there are no reports of...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612801619525
更新日期:2012-08-01 00:00:00
abstract::The physiological stability curve--a plot of the free energy of unfolding versus temperature--is calculated for hen egg white lysozyme from a combination of extrapolated unfolding thermodynamic data from reversible conditions and isothermal titrations with guanidine hydrochloride. The shape of the curve suggests the e...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606777841163
更新日期:2006-01-01 00:00:00
abstract::S-adenosyl-l-homocysteine hydrolase from a malaria parasite Plasmodium falciparum (PfSAHH) has been crystallized by the vapor diffusion method. The crystals belong to an orthorhombic space group P212121 with the cell dimensions of a = 76.66 A, b = 86.31 A, and c = 335.6 A. There are four subunits (one tetramer) per as...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866043478248
更新日期:2004-04-01 00:00:00
abstract::AB204 is an Activin/BMP2 chimera, which has been found to exhibit a higher activity than Bone Morphogenetic Protein 2 (BMP2) in osteogenic activity. To prepare AB204 for its preclinical studies, AB204 has been characterized in various formulation buffers. We observed that AB204 purified by ion-exchange chromatography ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986652105140218110302
更新日期:2014-05-01 00:00:00
abstract::Comparison between the BAD complexes indicated that BAD all docks a hydrophobic surface of PKAc regardless of its phosphorylation. PKAc may prevent Bcl-xL from rebinding to BAD by phosphorylating human BAD at Ser118; whereas human BAD is phosphorylated on Ser75 in a BAD-Bcl-xL complex, resulting in the dissociation of...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610790226003
更新日期:2010-02-01 00:00:00
abstract::This study revealed that the content of protein S29 in ribosomes of cancer cell line A549 was distinctly low (equivalent to about 30% of that of 2BS). The conclusion was acquired based on the ratios of spot volume of ribosomal protein S29 to that of several other ribosomal proteins (S29/L37a, S29/L38, S29/S27 and S29/...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866033408273
更新日期:2003-02-01 00:00:00
abstract::A 26-kDa trypsin inhibitor with an N-terminal sequence resembling storage proteins was purified from moth beans (Phaseolus acutifolius). It dose-dependently inhibited trypsin with an IC(50) value of about 0.38 microM. It inhibited [methyl-(3)H] thymidine incorporation by lymphoma MBL2 cells with an IC(50) value of 20 ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610791190408
更新日期:2010-06-01 00:00:00