Abstract:
:(-)-Sabinene is the major monoterpene produced by a European strain of the liverwort Conocephalum conicum. A cell-free extract from in vitro cultured plants catalysed the cyclization of geranyl diphosphate to sabinene. The responsible monoterpene cyclase was partially purified and characterized as an operationally soluble enzyme of M(r) 65,000, with a pH optimum at 7.5 and a requirement for a divalent metal ion as the only cofactor, with Mg2+ preferred. The general properties of the sabinene synthase from C. conicum resemble those of other monoterpene cyclases from gymnosperms and angiosperms. A North American strain of the liverwort produces (+)-bornyl acetate as the major monoterpene and it was demonstrated that bornane-type monoterpenes are derived from geranyl diphosphate in this liverwort, as in higher plants, by the action of bornyl diphosphate synthase.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Adam KP,Croteau Rdoi
10.1016/s0031-9422(97)00741-3subject
Has Abstractpub_date
1998-09-01 00:00:00pages
475-80issue
2eissn
0031-9422issn
1873-3700pii
S0031942297007413journal_volume
49pub_type
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