Abstract:
:Human pluripotent stem cells (hPSCs), i.e. human embryonic stem cells and human induced pluripotent stem cells, are able to self-renew and differentiate into multiple cell types. Because of these abilities, numerous attempts have been made to utilize hPSCs in regenerative medicine/cell therapy. hPSCs are, however, also tumorigenic, that is, they can give rise to the progressive growth of tumor nodules in immunologically unresponsive animals. Therefore, assessing and managing the tumorigenicity of all final products is essential in order to prevent ectopic tissue formation, tumor development, and/or malignant transformation elicited by residual pluripotent stem cells after implantation. No detailed guideline for the tumorigenicity testing of hPSC-derived products has yet been issued for regenerative medicine/cell therapy, despite the urgent necessity. Here, we describe the current situations and issues related to the tumorigenicity testing of hPSC-derived products and we review the advantages and disadvantages of several types of tumorigenicity-associated tests. We also refer to important considerations in the execution and design of specific studies to monitor the tumorigenicity of hPSC-derived products.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Kuroda T,Yasuda S,Sato Ydoi
10.1248/bpb.b12-00970subject
Has Abstractpub_date
2013-01-01 00:00:00pages
189-92issue
2eissn
0918-6158issn
1347-5215pii
DN/JST.JSTAGE/bpb/b12-00970journal_volume
36pub_type
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pub_type: 杂志文章
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