Abstract:
:Lipid droplets (LDs) are intracellular storage sites of neutral lipids, which accumulate in fatty liver disease. Here, we investigated the effects of fatty acids and glucose on LD formation in a cultured human hepatocyte, HuH7, by adding them to culture media. Fatty acids with carbohydrate chains C12-C18 efficiently induced LDs, but those of C8 and C10 were ineffective. Glucose did not induce LD formation even in the presence of insulin. Oleic acid induced significant increases in cellular neutral lipids, and cell fractionation revealed that most of the newly synthesized neutral lipids were concentrated in LDs together with LD proteins. The LD formation was not abrogated by removal of medium glucose but was significantly inhibited by an ACSL inhibitor, triacsin C. These results demonstrate that long-chain fatty acids contribute to LD formation to a greater extent than glucose, possibly by being taken up into the cells, activated by ACSL, reconstituted into neutral lipids and then stored in LDs. Pregnenolone and lithium did not suppress oleic acid-dependent LD formation, despite previous reports of their ability to inhibit LD formation in macrophages and adipocytes suggesting differences among LD formations in these cells.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Fujimoto Y,Onoduka J,Homma KJ,Yamaguchi S,Mori M,Higashi Y,Makita M,Kinoshita T,Noda J,Itabe H,Takanoa Tdoi
10.1248/bpb.29.2174subject
Has Abstractpub_date
2006-11-01 00:00:00pages
2174-80issue
11eissn
0918-6158issn
1347-5215pii
JST.JSTAGE/bpb/29.2174journal_volume
29pub_type
杂志文章abstract::Alzheimer's disease (AD) is the most common cause of dementia, with progressive memory impairment. Recently, neprilysin, a β-amyloid (Aβ)-degrading enzyme has become featured as a drug target for AD. Previously, we identified nobiletin from citrus peels as a natural compound possessing anti-dementia activity. In addit...
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