Adhesion of enterohemorrhagic Escherichia coli O157:H7 to the intestinal epithelia is essential for inducing secretory IgA antibody production in the intestine of mice.


:We examined whether adherence of enterohemorrhagic Escherichia coli (EHEC) O157:H7 to intestinal epithelial cells contributed to the induction of secretory immunoglobulin A (IgA) antibody production in mice. Wild-type EHEC O157:H7 and its mutants deficient in the espA, sepL, tir and eae genes, encoding adherent factors on the locus of enterocyte effacement (LEE), were inoculated intragastrically into mice. Inoculation of wild-type EHEC induced fecal IgA antibodies specific to EHEC at 4 weeks after the inoculation, but that of espA- and sepL-deletion mutants did not. Furthermore, even 4 inoculations at weekly intervals with espA-deletion mutant, heat-killed wild-type EHEC and nonpathogenic E. coli did not induce fecal IgA antibodies, although these bacterial inoculations induced serum antibodies. Kanamycin (Km)-treated mice showed prolonged and similar fecal shedding of Km-resistant mutants of EHEC O157:H7 including A2-F6 having intact LEE, A6-E7 (sepL-insertion mutant), G1-E11 (tir-insertion mutant) and Δeae (eae-deletion mutant). In this case, A2-F6 induced fecal IgA antibodies, but the other mutants with defective LEE did not. In contrast to the fecal IgA antibodies, serum IgM and IgG antibodies were induced in mice inoculated with any of the LEE defective mutants as well as A2-F6. Thus, adhesion of EHEC to epithelial cells is essential for inducing the mucosal immune response in the intestine but not for inducing the systemic immune response.


Biol Pharm Bull


Nagano K,Taguchi K,Tokoro S,Tatsuno I,Mori H




Has Abstract


2014-01-01 00:00:00














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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Oka M,Negishi M,Yamamoto T,Satoh K,Hirohashi T,Ichikawa A

    更新日期:1994-01-01 00:00:00

  • Effects of certain antiallergic drugs on experimental conjunctivitis in guinea pigs.

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kamei C,Izushi K,Nakamura S

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Lee MH,Kim JY,Ryu JH

    更新日期:2005-12-01 00:00:00

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Umehara K,Shimokawa Y,Miyamoto G

    更新日期:2004-07-01 00:00:00

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Takeo S,Miyake-Takagi K,Kikuchi K,Ando T,Ichikawa T,Omino K,Kajihara H,Takagi N,Tanonaka K

    更新日期:2003-03-01 00:00:00

  • Tissue-Clearing Techniques Enable Three-Dimensional Visualization of Aerosolized Model Compound and Lung Structure at the Alveolar Scale.

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Togami K,Kitayama A,Daisho T,Wang R,Tada H,Chono S

    更新日期:2018-01-01 00:00:00

  • Cloning of polyketide synthase genes from amphidinolide-producing dinoflagellate Amphidinium sp.

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kubota T,Iinuma Y,Kobayashi J

    更新日期:2006-07-01 00:00:00

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Aikawa N,Kishibayashi N,Karasawa A,Ohmori K

    更新日期:2000-11-01 00:00:00

  • Involvement of Tumor Lymphatic System in Translocation of Intratumorally Injected Liposomes.

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Shimizu K,Ikeuchi SY,Okajima A,Nakamura G,Muraoka E,Oku N

    更新日期:2018-01-01 00:00:00

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Liu T,Zheng Q,Qian Z,Wang H,Liu Z,Ren W,Zhang X,Han J

    更新日期:2016-05-01 00:00:00

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Pokharel YR,Liu QH,Oh JW,Woo ER,Kang KW

    更新日期:2007-06-01 00:00:00

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Nojima Y,Iguchi K,Suzuki Y,Sato A

    更新日期:2009-03-01 00:00:00

  • Dibenzylbutyrolactone lignans from Forsythia koreana fruits attenuate lipopolysaccharide-induced inducible nitric oxide synthetase and cyclooxygenase-2 expressions through activation of nuclear factor-κb and mitogen-activated protein kinase in RAW264.7 ce

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Lee JY,Cho BJ,Park TW,Park BE,Kim SJ,Sim SS,Kim CJ

    更新日期:2010-01-01 00:00:00

  • Laennec protects murine from concanavalin A-induced liver injury through inhibition of inflammatory reactions and hepatocyte apoptosis.

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Wu J,Yang T,Wang C,Liu Q,Yao J,Sun H,Kaku T,Liu KX

    更新日期:2008-11-01 00:00:00

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ban JY,Nguyen HT,Lee HJ,Cho SO,Ju HS,Kim JY,Bae K,Song KS,Seong YH

    更新日期:2008-01-01 00:00:00

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 临床试验,杂志文章


    authors: Katoh Y,Uchida S,Kawai M,Takei N,Mori N,Kawakami J,Kagawa Y,Yamada S,Namiki N,Hashimoto H

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Wu CZ,Inoue M,Ogihara Y

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Widy-Tyszkiewicz E,Piechal A,Joniec I,Blecharz-Klin K

    更新日期:2002-10-01 00:00:00

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Nakagawa S,Kojima Y,Sekino K,Yamato S

    更新日期:2013-01-01 00:00:00

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yao K,Karasawa A

    更新日期:1994-04-01 00:00:00

  • The influence of glycyrrhiza and antibiotics on the purgative action of sennoside a from Daiokanzoto in mice.

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Matsui E,Takayama K,Sato E,Okamura N

    更新日期:2011-01-01 00:00:00

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kume T,Suenaga A,Izumi Y,Akaike A

    更新日期:2016-01-01 00:00:00

  • Prophylactic Oral Administration of Magnesium Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice through a Decrease of Colonic Accumulation of P2X7 Receptor-Expressing Mast Cells.

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ohbori K,Fujiwara M,Ohishi A,Nishida K,Uozumi Y,Nagasawa K

    更新日期:2017-01-01 00:00:00

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Onoue Y,Kuwatsuka K,Miyazaki I,Asanuma M,Kitamura Y,Sendo T

    更新日期:2014-01-01 00:00:00

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Miyai E,Yanagida M,Akiyama J,Yamamoto I

    更新日期:1997-06-01 00:00:00

  • Effect of ginseng radix on GLUT2 protein content in mouse liver in normal and epinephrine-induced hyperglycemic mice.

    abstract::The oral administration of the water extract of Ginseng Radix (GR) to normal and epinephrine-induced hyperglycemic mice caused a significant decrease in the blood glucose level 4 h after its administration. The hepatic content of facilitative glucose transporter isoform 2, liver type glucose transporter (GLUT2) protei...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ohnishi Y,Takagi S,Miura T,Usami M,Kako M,Ishihara E,Yano H,Tanigawa K,Seino Y

    更新日期:1996-09-01 00:00:00

  • Properties and human origin of two angiotensin-I-converting enzyme inhibitory peptides isolated from a tryptic hydrolysate of human serum albumin.

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Nakagomi K,Ebisu H,Sadakane Y,Fujii N,Akizawa T,Tanimura T

    更新日期:2000-07-01 00:00:00

  • Inhibitory effects of a nitric oxide synthase inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME), on 2-deoxy-D-glucose-induced hyperglycemia in rats.

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Sugimoto Y,Yamada J,Yoshikawa T,Horisaka K

    更新日期:1997-12-01 00:00:00

  • Hypotensive activity and toxicology of constituents from Bombax ceiba stem bark.

    abstract::A novel constituent, shamimicin, 1"', 1"'-bis-2-(3,4-dihydroxyphenyl)-3,4-dihydro-3,7-dihydroxy-5-O-xylopyranosyloxy-2H-1-benzopyran along with lupeol, which possesses potent hypotensive activity, has been isolated from Bombax ceiba stem bark. BCBMM--one of the most active hypotensive fractions has revealed its advers...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Saleem R,Ahmad SI,Ahmed M,Faizi Z,Zikr-ur-Rehman S,Ali M,Faizi S

    更新日期:2003-01-01 00:00:00