Identification of the substrates for plasma hyaluronan binding protein.

Abstract:

:Plasma hyaluronan biding protein (PHBP) is a novel serine protease, which has an amino acid sequence homology to that of hepatocyte growth factor activator (HGFA), and has a similar domain structure to that of urinary plasminogen activator (u-PA), found in human plasma. We searched the PHBP substrate in human plasma by measuring the digested protein bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that fibrinogen and fibronectin were the major substrates of PHBP. PHBP cleaved the alpha-chain at multiple sites and the beta-chain between lysine53 and lysine54 but not the gamma-chain of fibrinogen. Therefore, PHBP did not initiate the formation of the fibrin clot and did not cause the fibrinolysis directly. PHBP did not cleave (activate) prothrombin and plasminogen, but it converted the inactive single chain urinary plasminogen activator to the active two chain form.

journal_name

Biol Pharm Bull

authors

Choi-Miura NH,Yoda M,Saito K,Takahashi K,Tomita M

doi

10.1248/bpb.24.140

subject

Has Abstract

pub_date

2001-02-01 00:00:00

pages

140-3

issue

2

eissn

0918-6158

issn

1347-5215

journal_volume

24

pub_type

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