Abstract:
:Staphylococcus epidermidis strains were isolated from the expressed human breast milk (EHM) of 14 healthy donor mothers. Genetic diversity was evaluated using RAPD-PCR REP-PCR and pulse-field gel electrophoresis (PFGE). PFGE allowed the best discrimination of the isolates, since it provided for the greatest diversity of the analyzed genomes. Among the S. epidermidis strains, resistance to gentamicin, tetracycline, erythromycin, clindamycin or vancomycin was detected, whilst four isolates were multiresistant. The results from our study demonstrate that staphylococci from EHM could be reservoirs of resistance genes, since we showed that tetK could be transferred from EHM staphylococci to Gram-negative Escherichia coli. Most of the staphylococcal strains displayed excellent proteolytic and lipolytic activities. Additionally, the presence of ica genes, which was related to their ability to form a biofilm on tissue culture plates, and the presence of virulence factors including autolysin/adhesin AtLE, point to their pathogenic potential.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Begović J,Jovčić B,Papić-Obradović M,Veljović K,Lukić J,Kojić M,Topisirović Ldoi
10.1016/j.micres.2012.09.004subject
Has Abstractpub_date
2013-02-22 00:00:00pages
77-83issue
2eissn
0944-5013issn
1618-0623pii
S0944-5013(12)00104-8journal_volume
168pub_type
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