Abstract:
:Nidus Vespae (honeycomb) is a kind of traditional Chinese medicine that has been demonstrated to inhibit the growth and acid-production of oral cariogenic bacteria. Subsequent studies showed that the chloroform/methanol (Chl/MeOH) chemical extraction of Nidus Vespae was the most effective inhibitor of growth and acidogenicity of Streptococcus mutans. In this study, we isolated the chemical compounds of the Nidus Vespae Chl/MeOH extraction, tested their antimicrobial activity against six cariogenic bacteria and further evaluated the acid inhibition properties, anti-F-ATPase activity and anti-LDH activity against S. mutans. The isolated flavonoids, quercetin and kaempferol, inhibited the growth of bacteria (S. mutans, Streptococcus sobrinus, Streptococcus sanguis, Actinomyces viscosus, Actinomyces naeslundii and Lactobacillus rhamnosus) with minimum inhibitory concentrations (MICs) ranging from 1 to 4 mg/ml and minimum bactericidal concentrations (MBCs) from 4 to 16 mg/ml. In addition, quercetin and kaempferol at sub-MIC levels significantly inhibited acidogenicity and acidurity of S. mutans cells. Treated with the test agents, the F-ATPase activity was reduced by 47.37% with 1mg/ml quercetin and by 49.66% with 0.5mg/ml kaempferol. The results showed that quercetin and kaempferol contained in Chl/MeOH extraction presented remarkably biological activity, suggesting that Nidus Vespae might be useful as a potential preventive and therapeutic agent in dental caries.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Guan X,Zhou Y,Liang X,Xiao J,He L,Li Jdoi
10.1016/j.micres.2011.03.002subject
Has Abstractpub_date
2012-01-20 00:00:00pages
61-8issue
2eissn
0944-5013issn
1618-0623pii
S0944-5013(11)00024-3journal_volume
167pub_type
杂志文章abstract::A polygalacturonase with a molecular mass of 74 kDa, an isoelectric point around pH 4.2 and pH--and temperature optima of 3.9 and 50 degrees C, respectively, was purified from a culture fluid of Penicillium frequentans. The enzyme was characterized as an exo-alpha-1,4-polygalacturonase (exo-PG I). Km and Vmax for sodi...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1078/0944-5013-00127
更新日期:2002-01-01 00:00:00
abstract::The endophytic bacteria were isolated from coffee roots and seeds in Vietnam and identified with 16S rDNA sequencing as belonging to the Actinobacteria, Firmicutes and Proteobacteria phyla with the Nocardia, Bacillus and Burkholderia as dominant genera, respectively. Out of the thirty genera recovered from Coffea cane...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126613
更新日期:2021-01-01 00:00:00
abstract::Microorganisms often suffer from oxidative stress created from nutrient starvation and environmental changes. Thioredoxin (Trx) and glutathione (GSH) pathways are believed critical in related protective functions. The roles of Trx in improving abiotic stress resistance in Trichoderma reesei are still unclear. In this ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2015.01.005
更新日期:2015-02-01 00:00:00
abstract::Eight ectomycorrhizal fungal isolates of Pisolithus associated with Eucalyptus species in different parts of India were collected and the genetic variability of these isolates was studied by ITS-RFLP and ITS sequencing. All the isolates showed same RFLP patterns with each restriction enzyme, indicating all these isola...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2004.03.003
更新日期:2004-01-01 00:00:00
abstract::Pseudomonas aeruginosa is one of the most common pathogens associated with nosocomial infections and a great concern to immunocompromised individuals especially in the cases of cystic fibrosis, AIDS and burn wounds. The pathogenicity of P. aeruginosa is largely directed by the quorum sensing (QS) system. Hence, QS may...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2019.05.001
更新日期:2019-09-01 00:00:00
abstract::Bitespiramycin (biotechnological spiramycin, Bsm) is a new 16-membered macrolide antibiotic produced by Streptomyces spiramyceticus WSJ-1 integrated exogenous genes. The gene cluster for Bsm biosynthesis consists of two parts: spiramycin biosynthetic gene cluster (92 kb) and two exogenous genes including 4"-O-isovaler...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126532
更新日期:2020-11-01 00:00:00
abstract::Staphylococcus saprophyticus is a gram-positive microorganism responsible for urinary tract infections (UTIs). Although some virulence factors are characterized, such as urease, autolysins, adhesins and hemagglutinins, large-scale proteomic studies have not been performed within this species. We performed the characte...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2018.08.008
更新日期:2018-11-01 00:00:00
abstract::Glycosyl hydrolase (GH) family 18 chitinases (Chi) and family 33 chitin binding proteins (CBPs) from Bacillus thuringiensis serovar kurstaki (BtChi and BtCBP), B. licheniformis DSM13 (BliChi and BliCBP) and Serratia proteamaculans 568 (SpChiB and SpCBP21) were used to study the efficiency and synergistic action of BtC...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2013.01.006
更新日期:2013-08-25 00:00:00
abstract::Aneurinibacillus migulanus strains Nagano and NCTC 7096 show potential in biocontrol against fungal and fungus-like plant pathogens, including a wide range of Ascomycota, Basidiomycota, and Oomycetes. Differences in terms of the range of pathogens that each strain inhibits, however, suggested that production of a sing...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2015.10.007
更新日期:2016-01-01 00:00:00
abstract::We have examined the capacity of Halobacterium halobium and Sulfolobus solfataricus to withstand the lethal effect of hydrogen peroxide and N-methyl-N'-nitro-N-nitrosoguanidine [corrected]. We tested a variety of pretreatment regimens with both mutagens and all failed to elicit an inducible response to the lethal eff...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/s0944-5013(97)80037-7
更新日期:1997-09-01 00:00:00
abstract::Quorum sensing (QS) systems, which depend on N-acylhomoserine lactone (AHL) signal molecules, mediate the production of virulence factors in many pathogenic microorganisms. One hundred and forty-six bacterial strains, isolated from a bivalve hatchery, were screened for their capacity to degrade five synthetic AHLs [N-...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2013.04.011
更新日期:2013-11-07 00:00:00
abstract::Araucaria angustifolia, a unique species of this genus that occurs naturally in Brazil, has a high socio-economic and environmental value and is critically endangered of extinction, since it has been submitted to intense predatory exploitation during the last century. Root-associated bacteria from A. angustifolia were...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2011.03.003
更新日期:2012-01-20 00:00:00
abstract::The genus Enterococcus belong to the genera of bacteria that produce lactic acid and can confer health benefits to living organisms. Selenium (Se) is an essential micronutrient for humans and animals. Thirty-six Enterococcus species isolated from dairy products were screened for Se(IV) sorption capacity for use as a p...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2010.03.005
更新日期:2011-03-20 00:00:00
abstract::Shewanella putrefaciens was grown on a series of ten alternate compounds as sole terminal electron acceptor. Each cell type was analyzed for Fe(III) reduction activity, absorbance maxima in reduced-minus-oxidized difference spectra and heme-containing protein content. High-rate Fe(III) reduction activity, pronounced d...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/S0944-5013(00)80042-7
更新日期:2000-07-01 00:00:00
abstract::Bacterial utilization of monomers is recognized as an important step in the biogeochemical cycling of organic matter. In this study we have compared the heterotrophic activity of bacterial communities from different micro-habitats within a salt marsh environment (Ria de Aveiro, Portugal) in order to establish spatial ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2005.02.001
更新日期:2005-01-01 00:00:00
abstract::We have cloned a laccase gene fragment isolated from a Trametes versicolor strain in Korea. It showed high similarity in nucleotide sequences when compared with other fungal laccases. TNT (2,4,6-trinitrotoluene), a widely used explosive, was transformed rapidly by T. versicolor. When TNT and its catabolic intermediate...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2005.12.001
更新日期:2006-01-01 00:00:00
abstract::The progression of life in all forms is not only dependent on agricultural and food security but also on the soil characteristics. The dynamic nature of soil is a direct manifestation of soil microbes, bio-mineralization, and synergistic co-evolution with plants. With the increase in world's population the demand for ...
journal_title:Microbiological research
pub_type: 杂志文章,评审
doi:10.1016/j.micres.2017.08.016
更新日期:2018-01-01 00:00:00
abstract::Foxing of paper is a deterioration phenomenon occurring in the form of brown-yellowish spots, the abiotic and/or biotic causes of which are not yet completely understood. Nevertheless, microbiological infection has been recognized that may contribute to paper damage and therefore it becomes important to know the taxon...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2007.06.002
更新日期:2008-01-01 00:00:00
abstract::In this study, we evaluated the efficiency of six isolates of Saccharomyces cerevisiae in controlling Colletotrichum acutatum, the causal agent of postbloom fruit drop that occur in pre-harvest citrus. We analyzed the mechanisms of action involved in biological control such as: production of antifungal compounds, nutr...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2015.04.003
更新日期:2015-06-01 00:00:00
abstract::In Salmonella Typhimurium (S. Typhimurium), lipopolysaccharide (LPS) anchored on the bacterial outer membrane is a major immune stimulus that can broadly activate immune cells and induce innate immune responses. wzxE is involved in bacterial LPS biosynthesis but has rarely been reported in Salmonella; wzxE encodes a f...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126502
更新日期:2020-09-01 00:00:00
abstract::The functions of two long-chain fatty acid CoA ligase genes (facl) in crude oil-degrading Geobacillus thermodenitrificans NG80-2 were characterized. Facl1 and Facl2 encoded by GTNG_0892 and GTNG_1447 were expressed in Escherichia coli and purified as His-tagged fusion proteins. Both enzymes utilized a broad range of f...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2012.05.001
更新日期:2012-12-20 00:00:00
abstract::Alcaligenes sp. strain O-1 inducibly deaminates 2-aminobenzenesulfonate (ABS) via dioxygenation to 3-sulfocatechol, which is desulfonated during meta ring-cleavage to yield 2-hydroxymuconate. This intermediate is transformed through the oxalocrotonate-branch of the sulfocatechol meta-pathway (Scm). The complete pathwa...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2009.05.006
更新日期:2010-05-30 00:00:00
abstract::Forty-one Lactobacillus strains were tested for antagonistic activity against nine strains of Vibrio. L. plantarum and L. casei were the most effective, and L. brevis was the least effective in inhibiting the growth of Vibrio species. L. gasseri and L. helveticus strains showed higher activity, while L. reuteri and L....
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/s0944-5013(98)80011-6
更新日期:1998-11-01 00:00:00
abstract::Central southern Mexico contains highly diverse legumes. In this study, nodule-associated bacteria (NAB) were isolated from wild legume nodules and from nodules on Phaseolus vulgaris plants used as a plant-trap in soils from the same areas as the wild legumes. The bacteria were identified through the 16S rRNA gene seq...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126522
更新日期:2020-10-01 00:00:00
abstract::Chaperone DnaK and its co-chaperone DnaJ plays various essential roles such as in assisting in the folding of nascent peptides, preventing protein aggregation and maintaining cellular protein homeostasis. Global transcriptional changes in vivo associated with deletion of dnaKJ were monitored using DNA microarray to el...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2016.03.001
更新日期:2016-05-01 00:00:00
abstract::Bacillus subtilis EA-CB0575 is a plant growth-promoting bacterium (PGPB) associated with banana and tomato crops. Root colonization is an important trait for PGPB microorganisms and potentiates the bacterial effect related to the mechanisms of plant growth promotion. Therefore, detection of bacterial colonization of r...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2018.08.017
更新日期:2018-12-01 00:00:00
abstract::A microbial consortium reducing high level of perchlorate was developed and in a fed batch bioreactor using acetate as substrate perchlorate was reduced at 0.25 g/g vss. day. Under stable performance, the microbial community structure of the reactor was analyzed through molecular and phenotypic methods. The diversity ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2015.04.011
更新日期:2015-08-01 00:00:00
abstract::Three classes of cell wall-defective mutants of the unicellular green alga Chlamydomonas reinhardtii have been described in the literature differing with respect to the amounts of cell wall material and its attachment to the plasma membrane, respectively. We have compared the production of the chaotrope-soluble cell w...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/S0944-5013(97)80012-2
更新日期:1997-07-01 00:00:00
abstract::Salmonella enterica serovar Enteritidis (S. Enteritidis, SE) is a major cause of foodborne diseases for humans. The completeness of the O-chain antigen of Lipopolysaccharide (LPS) determines whether a S. Enteritidis strain is smooth or rough. However, genes that are involved in the synthesis of LPS and rough-smooth va...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2018.05.001
更新日期:2018-01-01 00:00:00
abstract::Extended spectrum beta-lactamases (ESBLs) usually associated with multiple drug resistance, including beta-lactam and non-beta-lactam antibiotics. This resistance can cause Limitation in the choice of drugs appropriate for using in clinical practice, especially in life-threatening infections. In this study we aimed to...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2004.10.001
更新日期:2005-01-01 00:00:00