Abstract:
CONTEXT:The extraction method and the crude wound healing effects of sacchachitin from Ganoderma tsugae Murr. (Ganodermataceae) has been cited. However, its purity is still largely limited. OBJECTIVE:An improvement of the fractionation protocol to purify the sacchachitin from Ganoderma lucidum L. (Ganodermataceae) (SGL) is needed. METHODS:Fruiting bodies were extracted with double distilled water and subsequently the residue treated with 95% ethanol and then 40% ethanol. After being filtered, the pH of the supernatant was adjusted to 4.0 with 1 N HCl and lyophilized. The supernatant was added (3:1 v/v) ethanol, the precipitate was collected, 2% NaOH was added and refluxed. The supernatant was collected with pH adjusted to 4.0, then treated with 10% potassium hydroxide (KOH) with repeating acid precipitation and (3:1) ethanol precipitation twice more to obtain the sacchachitin. RESULTS:SGL had a hexosamine content 16.3% (w/w), firmly linked to a talomannan. Its Fourier Transform Infrared Spectroscopy (FTIR) spectrum revealed specific absorption (in cm(-1)) ν(O-H) 3455.5 b,s, amide ν(C=O) 1678.5, and amide I° δ(N-H) 1550.4. The percentage deacetylation degree was 37.6 and 39.4% for SGL and MSC, respectively. As contrast, MSC contained only 6.6% of hexosamine with a low protein/carbohydrate ratio 0.35 comparing to 0.82 for SGL. SGL was only moderately strong antioxidant regarding the anti-DPPH, antihydroxyl free radical, and antisuperoxide anion capabilities, exhibiting an IC(33) values of 10 mg/mL (the highest scavenging capability never exceeding 33%), 0.9 mg/mL, and 4.8 mg/mL, respectively. CONCLUSION:We have successfully isolated the pure sacchachitin from the fruiting bodies of G. lucidum that exhibits potent antioxidative activity and may be useful in fabrication of the artificial skin composite substitute.
journal_name
Pharm Bioljournal_title
Pharmaceutical biologyauthors
Chuang CM,Wang HE,Chang CH,Peng CC,Ker YB,Lai JE,Chen KC,Peng RYdoi
10.3109/13880209.2012.711840subject
Has Abstractpub_date
2013-01-01 00:00:00pages
84-95issue
1eissn
1388-0209issn
1744-5116journal_volume
51pub_type
杂志文章abstract:CONTEXT:Griflola frondosa (Fr) S.F. Gray (Meripilaceae) (GF) is a medical mushroom, and its regulation of the immune system is of interest for the treatment of mood disorders. α-Amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptors are the central mediator for the treatment of depression. OBJECTIVE:This...
journal_title:Pharmaceutical biology
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journal_title:Pharmaceutical biology
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doi:10.3109/13880209.2013.768273
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
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journal_title:Pharmaceutical biology
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journal_title:Pharmaceutical biology
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1129545
更新日期:2016-09-01 00:00:00
abstract::Context: Er Miao San (EMS) is a traditional Chinese medicine composed of Atractylodis Rhizoma and Phellodendri Cortex in a 1:1 weight ratio. EMS has been used to treat rheumatism in China for many years.Objective: To evaluate the anti-arthritic activity of EMS extract on adjuvant-induced arthritis (AA) in Sprague-Dawl...
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journal_title:Pharmaceutical biology
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journal_title:Pharmaceutical biology
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journal_title:Pharmaceutical biology
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更新日期:2012-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-02-01 00:00:00
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journal_title:Pharmaceutical biology
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更新日期:2012-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-02-01 00:00:00
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更新日期:2013-08-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2016-11-01 00:00:00
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更新日期:2015-01-01 00:00:00
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更新日期:2017-12-01 00:00:00
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更新日期:2016-09-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2010.523006
更新日期:2011-05-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2012.751616
更新日期:2013-05-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2014.943248
更新日期:2015-06-01 00:00:00
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pub_type: 杂志文章
doi:10.3109/13880209.2011.641023
更新日期:2012-07-01 00:00:00