Abstract:
CONTEXT:Granulocyte colony stimulating factor (G-CSF) has been commonly used to treat neutropenia caused by chemotherapy, radiotherapy, and organ transplants. Improved in vitro efficacy of G-CSF has already been observed by conjugating it to polyethylene glycol (PEG). OBJECTIVE:The in vivo bioassay using tetrazolium dye with the NFS-60 cell line has been recommended for G-CSF but no such monographs are available for PEGylated G-CSF in pharmacopeias. In the present study, the assay recommended for G-CSF was evaluated for its suitability to PEGylated G-CSF. MATERIALS AND METHODS:The generally used MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium]-based assay was compared with a bioassay employing a water-soluble tetrazolium dye, WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium], using NFS-60 cells at a concentration of 7 × 10(5) cells/ml against 800 IU/ml of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay was used to test the potency of various commercially available PEGylated G-CSF preparations. RESULTS:The results demonstrated enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line. CONCLUSION:Our study demonstrates the potential application of WST-8-based bioassays for other biotherapeutic proteins of human and veterinary interest.
journal_name
Pharm Bioljournal_title
Pharmaceutical biologyauthors
Tiwari K,Wavdhane M,Haque S,Govender T,Kruger HG,Mishra MK,Chandra R,Tiwari Ddoi
10.3109/13880209.2014.943248subject
Has Abstractpub_date
2015-06-01 00:00:00pages
849-54issue
6eissn
1388-0209issn
1744-5116journal_volume
53pub_type
杂志文章abstract:CONTEXT:Amphibian skins have wide variety of biologically active compounds associated with the natural defenses of these animals. OBJECTIVES:To study the in vitro anticancer activity of methanol extracts of the skin of Rhinella jimi Stevaux (Anura: Bufonidae). MATERIAL AND METHODS:The extract was obtained by cold met...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2012.655858
更新日期:2012-08-01 00:00:00
abstract::Context Gelidiella acerosa (Forsskål) Feldmann & G. Hamel (Rhodophyta-Gelidiales) is a marine red macroalga. Our previous work found that a benzene extract of G. acerosa possesses noticeable neuroprotective activity, when evaluated through in vitro and in vivo systems. Objective Bioactive-guided fractionation and iden...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2016.1145700
更新日期:2016-10-01 00:00:00
abstract:CONTEXT:Scrophularia genus (Scrophulariaceae) includes about 350 species commonly known as figwort. Many species of this genus grow wild in nature and have not been cultivated yet. However, some species are in danger of extinction. OBJECTIVE:This paper reviews the chemical compounds, biological activities and the ethn...
journal_title:Pharmaceutical biology
pub_type: 杂志文章,评审
doi:10.1080/13880209.2017.1397178
更新日期:2017-12-01 00:00:00
abstract:CONTEXT:Depression is one of the most common psychiatric diseases. Acorus tatarinowii Schott (Araceae) has shown many bioactivities in treatment of senile dementia and epilepsy. However, there is no report on antidepressant-like effects of the essential oil (EO) and its major components on animals under standardized ex...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2012.751616
更新日期:2013-05-01 00:00:00
abstract:CONTEXT:Vernonia amygdalina Del. (VA; Asteraceae or Compositae) is a small tree growing throughout tropical Africa. It is widely used for food and medicinal purposes by local people. It was reported that it had several qualities, including anticancer activity. OBJECTIVE:A sesquiterpene lactone, vernodalinol, was isola...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2010.523429
更新日期:2011-05-01 00:00:00
abstract:CONTEXT:Withania (Solanaceae) species are known to be a rich source of withanolides, which have shown several biological properties. OBJECTIVE:To identify the compounds responsible for Withania adpressa Coss. antioxidant activity and further test them for their NF-κB inhibition and antiproliferative activity in multip...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2018.1499781
更新日期:2018-12-01 00:00:00
abstract:CONTEXT:Vicenin-1, a flavonol glycoside, has potent platelet aggregation inhibition, antioxidant, radioprotectants and anti-inflammatory activities. OBJECTIVE:To establish a rapid, simple, precise and sensitive high-performance liquid chromatography (HPLC) method for determination of vicenin-1 in rat plasma, and to in...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2016.1172245
更新日期:2016-11-01 00:00:00
abstract:CONTEXT:Andrographis paniculata (Burm.f.) Nees (Acanthaceae) is widely used in tribal medicine in India and some other countries for multiple clinical applications. It contains andrographolide (AG) (diterpenoid lactone), a major phytomarker which probably accounts for its medicinal properties. OBJECTIVE:This study inv...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.836544
更新日期:2014-03-01 00:00:00
abstract:CONTEXT:During the last few decades, the prevalence of obesity in the western world has dramatically increased with epidemic proportions. Hand in hand with this statistic, the incidences of obesity-linked diseases such as diabetes are increasing with pandemic rate. The search for novel drugs and nutritional interventio...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2012.688838
更新日期:2012-12-01 00:00:00
abstract:CONTEXT:Herbal therapies are used worldwide to treat health conditions. In Mexico, generations have used them to treat gingivitis, periodontitis, mouth infections, and discoloured teeth. However, few studies have collected scientific evidence on their effects. OBJECTIVE:This study aimed at searching and compiling scie...
journal_title:Pharmaceutical biology
pub_type: 杂志文章,评审
doi:10.1080/13880209.2017.1347188
更新日期:2017-12-01 00:00:00
abstract::In the present study, the anxiolytic and sedative-hypnotic activities of polygalasaponins extracted from Polygala tenuifolia Willdenow (Polygalaceae) were determined in mice using hole-board, elevated plus maze, open field, and sodium pentobarbital-induced hypnosis tests. Moreover, the acute toxicity of polygalasaponi...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880200903280042
更新日期:2010-07-01 00:00:00
abstract::Context Previous studies have shown that Scutellariae Radix, the dried root of Scutellaria baicalensis Georgi (Labiatae), has a certain inhibitory effect on P-glycoprotein (P-gp), but the effects of its main active constituents on P-gp are still ambiguous. Objectives In vitro studies were performed to investigate the ...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1107744
更新日期:2016-09-01 00:00:00
abstract:CONTEXT:Several plant-derived natural products have been used in clinical phase for applications in neurological, cardiovascular, and inflammatory diseases. Arbutus andrachne L. (Ericacea) is an evergreen shrub native to the Mediterranean region. Traditionally, the fruits and leaves of Arbutus tree are well known and u...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1100638
更新日期:2016-01-01 00:00:00
abstract:CONTEXT:Liposomes are increasingly employed to deliver chemotherapeutic agents, antisense oligonucleotides, and genes to various therapeutic targets. OBJECTIVE:The present investigation evaluates the ability of fusogenic pH-sensitive liposomes of rapamycin in increasing its antiproliferative effect on human breast ade...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.871640
更新日期:2014-07-01 00:00:00
abstract:CONTEXT:Previous in vitro studies have demonstrated that emodin (1,3,8-trihydroxy-6-methyl-anthraquinone), an anthraquinone derivative from the rhizome of Rheum palmatum L., can inhibit the activation of P2X₇ receptors (P2X₇R) as a potential antagonist. However, the effects of emodin on P2X₇R-related inflammatory proce...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.810648
更新日期:2014-01-01 00:00:00
abstract:CONTEXT:Dicranopteris linearis (Burm.f.) Underw. (Gleicheniaceae) has been scientifically proven to exert various pharmacological activities. Nevertheless, its anti-proliferative potential has not been extensively investigated. OBJECTIVE:To investigate the anti-proliferative potential of D. linearis leaves and determi...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2018.1495748
更新日期:2018-12-01 00:00:00
abstract:CONTEXT:Amaranth and canola oils have been used traditionally. Amaranth has been identified as being of interest because of its outstanding nutritive value. Amaranth oil is a rich source of highly unsaturated fats and so could be a valuable dietary alternative for individuals affected with obesity. Reactive oxygen spec...
journal_title:Pharmaceutical biology
pub_type: 杂志文章,随机对照试验
doi:10.1080/13880209.2019.1569696
更新日期:2019-12-01 00:00:00
abstract::Several drugs of herbal origin are known to possess anxiolytic and antidepressant effects. In a recent study, we showed that extracts from Euphorbia hirta L. (Euphorbiaceae) (Eh) demonstrated anxiolytic effects in rats subjected to chronic immobilization stress (CIS) but not in rats that underwent forced swim stress (...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880200903188534
更新日期:2010-05-01 00:00:00
abstract:CONTEXT:Linum usitatissimum L. (Linaceae), commonly known as flaxseed, is a good source of dietary fiber and lignans. Earlier we reported cardioprotective, antihyperlipidemic, and in vitro antioxidant activity of flax lignan concentrate (FLC) obtained from flaxseed. OBJECTIVES:To isolate secoisolariciresinol diglucosi...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.877492
更新日期:2014-09-01 00:00:00
abstract:CONTEXT:Virgin coconut oil (VCO) has been reported to possess antioxidative, anti-inflammatory and anti-stress properties. OBJECTIVE:Capitalizing on these therapeutic effects, this study investigated for the first time the potential of VCO on memory improvement in vivo. MATERIALS AND METHODS:Thirty male Wistar rats (...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2017.1280688
更新日期:2017-12-01 00:00:00
abstract:CONTEXT:Diabetes mellitus is characterized by oxidative stress, which in turn induces endothelial dysfunction. As a potent antioxidant compound, lycopene might rescue diabetic endothelial dysfunction by reducing oxidative stress. OBJECTIVE:The present study investigated whether lycopene could lower oxidative stress an...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2011.574707
更新日期:2011-11-01 00:00:00
abstract::Heliopsis longipes S.F. Blake (Asteraceae: Heliantheae) (chilcuague) is used in Mexican traditional medicine against parasites and to alleviate tooth and muscle pains. Its biocide effect has already been experimentally demonstrated; however, its analgesic action and its action on the nervous system (NS) have not been ...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880200903078495
更新日期:2010-02-01 00:00:00
abstract:CONTEXT:Rutin, a flavonoid commonly present in onions, apples and tea, has been suggested to have a variety of pharmacological activities, including immunomodulator, anti-inflammatory and antioxidant activities. OBJECTIVES:The present study was to examine the protective effects of rutin on gastric mucosal damage induc...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.771375
更新日期:2013-07-01 00:00:00
abstract::Context Acetaminophen (APAP), also known as paracetamol and N-acetyl p-aminophenol, is one of the most frequently used drugs for analgesic and antipyretic purposes on a worldwide basis. It is safe and effective at recommended doses but has the potential for causing hepatotoxicity and acute liver failure (ALF) with ove...
journal_title:Pharmaceutical biology
pub_type: 杂志文章,评审
doi:10.3109/13880209.2016.1150302
更新日期:2016-10-01 00:00:00
abstract:CONTEXT:The genus Premna (Lamiaceae), distributed throughout tropical and subtropical Asia, Africa, Australia and the Pacific Islands, is used in folk medicine primarily to treat inflammation, immune-related diseases, stomach disorders, wound healing, and skin diseases. OBJECTIVES:This review exhaustively gathers avai...
journal_title:Pharmaceutical biology
pub_type: 杂志文章,评审
doi:10.1080/13880209.2017.1323225
更新日期:2017-12-01 00:00:00
abstract:CONTEXT:The nephron-protective efficacy of Abelmoschus manihot (Linn.) Medicus (Malvaceae) has been proved by randomized controlled clinical trial. OBJECTIVE:Flavonoids are main active components of A. manihot, which can be transformed into glucuronide/sulfate conjugates in vivo. Exploring the pharmacokinetic profile ...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1068337
更新日期:2016-01-01 00:00:00
abstract::Context Ginseng is a widely used herbal medicine in China but its mechanism of action remains unclear. Objective The objectives of this work were to study the protective effect of ginsenoside Rg1 on subacute murine renal damage induced by d-galactose and its mechanism. Materials and methods C57BL/6J mice were injected...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1129543
更新日期:2016-09-01 00:00:00
abstract::Context The underground edible tuber of Dioscorea alata L. (Dioscoreaceae) is a functional food with high nutritive value and therapeutic potential. The tuber is known to possess anti-inflammatory properties in traditional medicine. Objective The present study explores the anti-inflammatory activity and standardisatio...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1104702
更新日期:2016-08-01 00:00:00
abstract::Context Phillyrea latifolia L. (Oleaceae), commonly found in the Mediterranean region in Turkey, is used as medicinal teas for weight loss and hyperglycaemia in folk medicine. Objective The study investigated the possible effects of P. latifolia leaves aqueous extract's on weight loss and biochemical-histological chan...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1137952
更新日期:2016-10-01 00:00:00
abstract::Clitoria ternatea , commonly known as Shankpushpi, is widely used in the traditional Indian system of medicine as a brain tonic and is believed to promote memory and intelligence. We examined the effectiveness of alcoholic extracts of aerial and root parts of C. ternatea at 300 and 500 mg/kg doses orally in rats in at...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1076/1388-0209(200001)3811-BFT051
更新日期:2000-01-01 00:00:00