Abstract:
INTRODUCTION:Only automated phenotypic methods are currently used in Colombian hospitals for identifying isolates of the Acinetobacter calcoaceticus-A. baumannii complex (ACB). The phenotypical similarities in these species mean that they cannot be differentiated by manual or automated methods, thereby leading to their identification as A. baumannii, or ACB complex in clinical settings. Our objective was to identify to the species level 60 isolates, from four hospitals, evaluate their antibiotic susceptibility, and detect resistance-related genes. METHODS:16S-23S rRNA internal transcribed spacer (ITS) region and rpoB gene partial sequences were amplified. Resistance genes for cephalosporin, carbapenem and aminoglycoside were detected by PCR. Possible mutations in the quinolone resistance-determining region (QRDR) were evaluated. The association of ISAba-1 with blaOXA and blaADC genes was determined by PCR. Amplification products of ITS region, rpoB gene and some resistance genes were sequenced and compared using the BLAST tool. RESULTS:16S-23S rRNA ITS region and partial rpoB gene sequence analysis allowed 51isolates to be identified as A. baumannii, 8 as A. nosocomialis, and 1 isolate as A. pitti. A. baumannii isolates were highly resistant to all antibiotics tested, while the others were susceptible to ciprofloxacin and ampicillin/sulbactam. Quinolone resistance, found only in A. baumannii, was associated with mutations in the QRDR region of gyrA and parC genes. CONCLUSION:This is the first investigation in Colombia that has identified ACB complex species using molecular methods, and determined differences in antibiotic resistance and resistance genes among the species. It is of the highest importance to identify isolates to the species level for future resistance and epidemiology studies in our region.
journal_name
Enferm Infecc Microbiol Clinjournal_title
Enfermedades infecciosas y microbiologia clinicaauthors
Reguero MT,Medina OE,Hernández MA,Flórez DV,Valenzuela EM,Mantilla JRdoi
10.1016/j.eimc.2012.07.013subject
Has Abstractpub_date
2013-03-01 00:00:00pages
142-6issue
3eissn
0213-005Xissn
1578-1852pii
S0213-005X(12)00264-9journal_volume
31pub_type
杂志文章abstract::Sepsis is one of the main causes of mortality and morbidity in hospitals. Early detection of pathogens using nucleic acid-based techniques speeds diagnosis of bacteremia and/or fungemia, aids the rapid application of appropriate antibiotics, reduces the use of unnecessary antibiotics, and lowers mortality. Two commerc...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1016/s0213-005x(08)76544-3
更新日期:2008-07-01 00:00:00
abstract:OBJECTIVE:To determine the number of strains of classic enteropathogenic E. coli (EPEC) that have the eae gene, that is considered a pathogenicity factor. MATERIAL AND METHODS:The presence of the eae gene has been evaluated on 62 EPEC strains of ten different serogroups, isolated from children with gastroenteritis. R...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract:BACKGROUND:There are limited data on etravirine (ETV) use in routine clinical practice. METHODS:The reasons, efficacy and tolerability of its use are retrospectively reviewed. RESULTS:Overall, 151patients started a regimen containing ETV. The main reasons for prescription were toxicity and virological failure. After ...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2013.04.018
更新日期:2013-12-01 00:00:00
abstract::The duration of antibiotic treatment in patients with an infectious process is based on empirical considerations and those with intraabdominal infections are no exception. Therefore, the recommended duration of antibiotic therapy in intraabdominal infection is controversial and no consensus has been reached due to the...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1016/S0213-005X(10)70030-6
更新日期:2010-09-01 00:00:00
abstract:OBJECTIVES:To prospectively study the emergence and diagnostic problems of bronchiolitis caused by influenza A virus among children durign the 1999-2000 influenza epidemic. MATERIALS AND METHODS:Negative respiratory syncitial virus (RSV) specimens (ELISA dot-blot) were seeded in the MDCK cell line for the isolation of...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/s0213-005x(01)72703-6
更新日期:2001-12-01 00:00:00
abstract::Dyslipidemia is common in HIV-infected patients receiving antiretroviral therapy (ART) and it is often associated with the use of specific antiretroviral drugs. The phenotypic profile can include elevated triglycerides or cholesterol alone, or mixed patterns with varying changes in LDL and HDL lipoproteins, which impl...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1157/13095376
更新日期:2006-12-01 00:00:00
abstract:OBJECTIVE:Epidemiological and microbiological study of a salmonellosis outbreak, affecting 22 children in a nursery school in Oviedo (Spain). METHODS:Attack rates and epidemic curves were determined, and bacterial typing methods were applied. RESULTS:The outbreak was attributed to a Salmonella enterica serovar Typhim...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1157/13106962
更新日期:2007-06-01 00:00:00
abstract:INTRODUCTION:Streptococcus pneumoniae is a significant cause of morbidity and mortality. Children with certain conditions are at risk of developing pneumococcal disease, including invasive pneumococcal disease (IPD). The aim of this study is to estimate admission rates for IPD in children with risk conditions in Catalo...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2015.07.006
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journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 共识发展会议,杂志文章,实务指引,评审
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更新日期:2013-10-01 00:00:00
abstract::A survey was carried out of all urinary tract infections which developed bacteremia during an 18 month period. The number of episodes of bacteremia of urinary origin (BUO) detected in our institution during that period was 108. The urinary tract was the most common origin of the cases of community acquired (CA) bacter...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract:BACKGROUND:Spain, together with the other southern European countries, was considered to be an area with a moderate degree of endemia. This fact has consequences for tourists that visit these areas and for vaccination strategies. A prevalence study was proposed in order to get to know the situation of this infection in...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/s0213-005x(01)72686-9
更新日期:2001-11-01 00:00:00
abstract:INTRODUCTION:To describe clinical features, complications, serotypes and antibiotic resistance in pneumococcal pneumonia in our environment after the generalization of the heptavalent conjugate vaccine (PCV-7) in paediatrics. MATERIAL AND METHODS:Prospective study of episodes of pneumococcal pneumonia, with positive c...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2010.09.014
更新日期:2011-04-01 00:00:00
abstract::Pharmacogenetics, the study of how individual genetic profiles influence the response to drugs, is an important topic. Results from pharmacogenetics studies in various clinical settings may lead to personalized medicine. Herein, we present the most important concepts of this discipline, as well as currently-used study...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/s0213-005x(08)76506-6
更新日期:2008-05-01 00:00:00
abstract:OBJECTIVE:To study the possible viral etiology in 139 infants with lower respiratory tract infection who required hospitalization in the Infant Unit of our hospital, from October 1994 to June 1995. PATIENTS AND METHODS:139 patients were admitted, aged from 13 days to 14 months, during this period. The etiological agen...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract:INTRODUCTION:Invasive pneumococcal disease (IPD) typically presents as bacterial pneumonia, meningitis or primary bacteraemia. However, Streptococcus pneumoniae can produce infection at any level of the body (endocarditis, arthritis, spontaneous bacterial peritonitis, etc.), which is also known as unusual IPD (uIPD). T...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2017.05.003
更新日期:2018-05-01 00:00:00
abstract::We retrospectively analyze the untoward effects due to cotrimoxazole (CMX) when this drug was used as initial therapy in 37 Spanish AIDS patients with Pneumocystis carinii pneumonia (PCP). A total of 46% of patients developed an adverse reaction, that was considered severe in 24.3% of cases, and prompted to stop CMX t...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1991-08-01 00:00:00
abstract::The External Quality Control Program of the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC) include controls for bacteriology, serology, mycology, parasitology, mycobacteria, virology and molecular microbiology. This article presents the most relevant conclusions and lessons from the 2014 cont...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/S0213-005X(16)30212-9
更新日期:2016-07-01 00:00:00
abstract::We have evaluated the results of a serologic study of 34 patients with a possible chlamydial infection, obtaining three successive serum samples that were assessed with microimmunofluorescence (IIF) and complement fixation (CF) tests, so as to establish a comparison between the antibody titer measured by each of them....
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract:BACKGROUND:The aim of this study was to define the incidence of cervico-vaginal colonization by group B Streptococcus (SGB) in full term pregnancy as well as vertical and horizontal transmission in newborns attended in two public hospitals in Valencia, Venezuela. METHODS:An study cervico-vaginal exudates from 171 full...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00
abstract::Infections are one of the main complications that decisively affect the final outcome of transplants. Clinical microbiology laboratory has a key role in diagnosis, treatment and prevention of these complications. Centres with transplant programs must be technically supported with a well developed laboratory with speci...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2011.05.015
更新日期:2011-11-01 00:00:00
abstract::Staphylococcus aureus is a versatile organism with several virulent characteristics and resistance mechanisms at its disposal. It is also a significant cause of a wide range of infectious diseases in humans. S. aureus often causes life-threatening deep seated infections like bacteremia, endocarditis and pneumonia. Whi...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1157/13086552
更新日期:2006-03-01 00:00:00
abstract:BACKGROUND:To determine the characteristics of HIV-infection in foreign patients residing in Elche, Spain. METHOD:A retrospective comparative analysis of clinical and epidemiological characteristics was performed to study HIV-infection in foreign immigrants, foreigners who were not immigrants and the native Spanish po...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1157/13078824
更新日期:2005-10-01 00:00:00
abstract:INTRODUCTION:Diagnosis of HSV-1 keratitis (HK) is frequently based on clinical findings. Invasive specimens (corneal scrapings, biopsies) are required for microbiological diagnosis. METHODS:Corneal scrapings and conjunctival swabs were collected on patients with/without clinical suspicion of HK from 2007 to 2012. RES...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2013.07.005
更新日期:2014-01-01 00:00:00
abstract::The majority of healthcare associated infections are endemic, but outbreaks or epidemic infections also occur. The most frequent nosocomial endemic infections are catheter associated bacteremia, ventilator-associated pneumonia, surgical site infections and urinary catheter associated infections. These are conditions w...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2012.10.010
更新日期:2013-03-01 00:00:00
abstract::Quinolone resistance is caused mainly by chromosomal mutations in gram negative bacteria. In 1998, plasmid-mediated resistance to quinolones in clinical isolates was first reported in a Klebsiella pneumoniae strain. Locus qnr (quinolone resistance) was responsible of the quinolone resistance in this plasmid. qnr codes...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1157/13070406
更新日期:2005-01-01 00:00:00
abstract::Maraviroc is a selective and slowly reversible antagonist of the CCR5 co-receptor which has shown to have powerful antiviral activity, in vitro, against a wide range of HIV clinical isolates, including strains multi-resistant to 4 classes of pre-existing antiretroviral drugs. Maraviroc is active against HIV population...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1016/s0213-005x(08)76562-5
更新日期:2008-10-01 00:00:00
abstract:BACKGROUND:The aim of the present study was to know the usefulness of a enzyme immunoanalysis (EIA) technique for the monitorization of the serologic to treatment of infection by Helicobacter pylori in Spanish children. METHODS:17 children with digestive symptomatology in whom the diagnosis of infection by Helicobacte...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::The high genetic diversity of human immunodeficiency virus (HIV) poses a significant challenge to the diagnosis and microbiological characterization of HIV infection. Because of the continual emergence of new variants and the global spread of HIV groups, subtypes and recombinant forms, accurate diagnostic tools are of...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1157/13128783
更新日期:2008-11-01 00:00:00
abstract:BACKGROUND:This review summarizes the different species of dermatophytes isolates in our laboratory between 1991 and 1995. We describe the clinical forms and establish the distribution over this period of time. METHODS:Retrospective survey of samples from outpatients of the Dermatology Service in Miguel Servet Hospita...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract:OBJECTIVES:Cardiac surgery is a life-saving procedure in patients diagnosed with infective endocarditis (IE). There are several validated risk scores developed to predict early-mortality; nevertheless, long-term survival has been less investigated. The aim of the present study is to analyze the impact of IE-specific ri...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2018.10.017
更新日期:2019-01-01 00:00:00