Abstract:
OBJECTIVES:To prospectively study the emergence and diagnostic problems of bronchiolitis caused by influenza A virus among children durign the 1999-2000 influenza epidemic. MATERIALS AND METHODS:Negative respiratory syncitial virus (RSV) specimens (ELISA dot-blot) were seeded in the MDCK cell line for the isolation of influenza A virus, by the shell-vial technique. Vials were revealed by indirect immunofluorescence with antibodies directed against the influenza A virus nucleoprotein (Monofluookit IA, Pasteur Diagnostics). The clinical and epidemiologic features of patients with viral recovery were studied. A theoretical cost/benefit study was performed on the usefulness of the rapid antigenic detection of the influenza A virus. RESULTS:A total of 117 cases of respiratory infection caused by influenza A virus were detected. In 62 cases (52.9%) the diagnosis was bronchiolitis. Of them, 80.6% of cases were aged less than 6 years. Subtype H3N2 predominated in all respiratory infections, paticularly in bronchiolitis. During the 1999-2000 season, a significant emergence of bronchiolitis caused by influenza A virus was observed (week 3, 30% cases of influenza A versus 24% of RSV). The cost/benefit study disclosed that the antigenic detection of RSV and influenza A virus in all respiratory specimens would amount up to 30.83 Euros/specimen. CONCLUSIONS:During the last influenza epidemic, an emergence of bronchiolitis caused by influenza A virus among children was observed and confirmed. Based upon this observation, a dual strategy should be followed. On the one hand, a prophylactic approach (immunization of pregnant women); on the other hand, a diagnostic approach with a rapid antigenic detection of the influenza A virus.
journal_name
Enferm Infecc Microbiol Clinjournal_title
Enfermedades infecciosas y microbiologia clinicaauthors
Reina J,Ballesteros F,Mesquida X,Galmes M,Ferres F,Ruiz De Gopegui Edoi
10.1016/s0213-005x(01)72703-6subject
Has Abstractpub_date
2001-12-01 00:00:00pages
467-70issue
10eissn
0213-005Xissn
1578-1852pii
13023813journal_volume
19pub_type
杂志文章abstract::In the present study an immunofluorescence technique detecting antibody-coated bacteria was evaluated. It was applied to the sputum of seven patients with bacteremic pneumococcal pneumonia to assess its diagnostic usefulness. We conclude that it is a valid, quick and easy technique which permits to differentiate betwe...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract:OBJECTIVE:To determine the number of strains of classic enteropathogenic E. coli (EPEC) that have the eae gene, that is considered a pathogenicity factor. MATERIAL AND METHODS:The presence of the eae gene has been evaluated on 62 EPEC strains of ten different serogroups, isolated from children with gastroenteritis. R...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::We analyze the experience in serologic diagnosis of Lyme's borreliosis. From a total of 551 patients studied from 1987 to 1989, we further evaluate 80 cases with erythema chronicum migrans or a clinical diagnosis of Lyme's disease and positive serological tests. The techniques used were IFI, ELISA1 (Whittaker Bioprodu...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract:BACKGROUND:Extrapulmonary tuberculosis is a relatively frequent disease in patients with HIV infection. This may be observed coinciding with pulmonary involvement or without diagnostic data of the same. Within extrapulmonary involvement, gastrointestinal involvement is one of the least frequently observed and its diagn...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract::Quinolone resistance is caused mainly by chromosomal mutations in gram negative bacteria. In 1998, plasmid-mediated resistance to quinolones in clinical isolates was first reported in a Klebsiella pneumoniae strain. Locus qnr (quinolone resistance) was responsible of the quinolone resistance in this plasmid. qnr codes...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1157/13070406
更新日期:2005-01-01 00:00:00
abstract::Pharmacogenetics, the study of how individual genetic profiles influence the response to drugs, is an important topic. Results from pharmacogenetics studies in various clinical settings may lead to personalized medicine. Herein, we present the most important concepts of this discipline, as well as currently-used study...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/s0213-005x(08)76506-6
更新日期:2008-05-01 00:00:00
abstract:INTRODUCTION:Community-onset infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are increasing. However, there is little information about community-onset bacteremia (CB) due to MRSA in Spain. The objectives of this study were to evaluate the prevalence, clinical and molecular epidemiology, clinica...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,多中心研究
doi:10.1016/j.eimc.2009.07.004
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVE:To evaluate the efficacy and safety of cotrimoxazol plus rifampicin in staphylococcal osteoarticular infection. METHOD:Open, non-comparative study of adult hospitalized patients with documented staphylococcal bone infection. RESULTS:From Feb 1989 to Dec 1993 28 episodes of staphylococcal bone infection were...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the characteristics of the Campylobacter jejuni infection in HIV positive patients. DESIGN:We report all cases appeared in our hospital between September 1991 and October 1992 with: HIV infection, chronic diarrhoea and C. jejuni in stool culture. Epidemiologic factors, other opportunist infection...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
abstract:INTRODUCTION:The increase in infections due to dermatophytes in recent years led us to study the effectiveness of new antifungal formulations against these microorganisms. METHODS:The in vitro activity of a new antifungal agent, voriconazole, was compared with three other antifungal agents, itraconazole, fluconazole a...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/s0213-005x(03)72992-9
更新日期:2003-11-01 00:00:00
abstract:INTRODUCTION:In order to assess the relationship between the concentrations of airborne fungi and particles, particle counting was combined with fungal air sampling in several rooms of a hospital. METHODS:Concentrations of ≥0.5μm particles (P05) and ≥1μm particles (P1) were measured using a particle counter; fungal ai...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2012.01.005
更新日期:2013-04-01 00:00:00
abstract:OBJECTIVE:To study the possible viral etiology in 139 infants with lower respiratory tract infection who required hospitalization in the Infant Unit of our hospital, from October 1994 to June 1995. PATIENTS AND METHODS:139 patients were admitted, aged from 13 days to 14 months, during this period. The etiological agen...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract:INTRODUCTION AND AIM:Immigration to Spain of Latin Americans with Chagas disease in its indeterminate phase could result in vertical transmission of the disease or transmission by transfusion or organ transplantation. To ascertain the magnitude of this problem, we investigated the prevalence of bearers among women who ...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,多中心研究
doi:10.1016/s0213-005x(08)75276-5
更新日期:2008-12-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to know, through a national survey, the methods and techniques used for the diagnosis of Helicobacter pylori (Hp) in the different Clinical Microbiology Services/Laboratories in Spain, as well as antibiotic resistance data. METHODS:The survey requested information about the diagn...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2019.11.008
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVE:[corrected] a) To identify Citrobacter strains following the conventional biochemical reaction of Brenner and col; b) to evaluate the sensitivity and specificity of the O'Hara's method compared with Brenner's method, and c) to determine the rate and distribution of the strains in the clinical isolates. MATER...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/s0213-005x(01)72541-4
更新日期:2001-01-01 00:00:00
abstract::The External Quality Control Program of the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC) include controls for bacteriology, serology, mycology, parasitology, mycobacteria, virology and molecular microbiology. This article presents the most relevant conclusions and lessons from the 2014 cont...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/S0213-005X(16)30212-9
更新日期:2016-07-01 00:00:00
abstract:OBJECTIVE:Infectious pharyngotonsillitis is usually managed with antibiotics by general practitioners and pediatricians both in primary care and the emergency services. In the present work we try to assess the antibiotic variability and appropriateness in the management of acute pharyngotonsillitis among several emerge...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract:BACKGROUND:Antibiotics account for 30% of hospital pharmacy expenses. More than 50% of the prescriptions are considered inappropriate; hence, programs devoted to optimizing the prescription of antibiotics should be developed. We present the results of a non-compulsory program for the assessment and control of antibioti...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1157/13073141
更新日期:2005-04-01 00:00:00
abstract:BACKGROUND:Pseudomonas aeruginosa neurosurgical meningitis is a rare entity, usually related with intraventricular catheters and associated with high mortality rates. We describe the clinical characteristics, treatment and outcomes of a series of neurosurgical meningitis caused by P. aeruginosa along 1990-2016. METHOD...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2019.04.003
更新日期:2020-02-01 00:00:00
abstract:INTRODUCTION:To determine the cost and volume of work implied in providing a parasitological diagnosis when attending immigrants in a referral Tropical Medicine Unit. METHODS:The total number and type of samples, requests processed and parasitology laboratory costs were quantified after attending 1258 immigrants durin...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:2003-04-01 00:00:00
abstract::Staphylococcus aureus is a versatile organism with several virulent characteristics and resistance mechanisms at its disposal. It is also a significant cause of a wide range of infectious diseases in humans. S. aureus often causes life-threatening deep seated infections like bacteremia, endocarditis and pneumonia. Whi...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1157/13086552
更新日期:2006-03-01 00:00:00
abstract:INTRODUCTION:To describe clinical features, complications, serotypes and antibiotic resistance in pneumococcal pneumonia in our environment after the generalization of the heptavalent conjugate vaccine (PCV-7) in paediatrics. MATERIAL AND METHODS:Prospective study of episodes of pneumococcal pneumonia, with positive c...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2010.09.014
更新日期:2011-04-01 00:00:00
abstract::The recovery rate of Moraxella catarrhalis in a selective culture medium with acetazolamide and in a conventional blood-agar medium was compared from 1291 samples from the respiratory tract and conjunctivae of children and adults with respiratory and ocular symptomatology. M. catarrhalis was recovered in 215 samples o...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
abstract:BACKGROUND:We evaluate two different commercially available kits for detecting specific antibodies against Helicobacter pylori in a group of 48 patients with gastrointestinal symptoms that underwent an endoscopic procedure. We also study the seroprevalence of anti-H. pylori antibodies in asimptomatic individuals from o...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to develop a satisfactory technique to detect punctual mutations in blaTEM genes. METHODS:The strains [E. coli HB 101 pBR322 (TEM-1), E. coli J62 RP4 (TEM-2)] were submitted to PCR with primers PL1 and PL2 which amplify the genetic region susceptible of punctual mutations. Then...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract:INTRODUCTION:The pathophysiological changes that determine the severity of dengue are still not well known, therefore it is important to study the probable relationship with the host genetic. METHODS:We analyzed the possible association between the FcγRIIa polymorphism and clinical signs in individuals who suffered de...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2012.05.011
更新日期:2012-12-01 00:00:00
abstract:INTRODUCTION:Primary lymphoma is the most common neoplasm of the central nervous system (CNS) in AIDS patients. METHODS:We retrospectively reviewed the clinical manifestations, neuroimaging findings, diagnostic methods used, histological characteristics, detection of Epstein-Barr virus (EBV) DNA by PCR in cerebrospina...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1016/s0213-005x(04)73105-5
更新日期:2004-06-01 00:00:00
abstract:BACKGROUND:The genetic and molecular mechanisms involved in antimicrobial resistance of 10 strains of gramnegative bacilli (1 Serratia marcescens; 2 Escherichia coli; 1 Proteus mirabilis; 4 Klebsiella pneumoniae; 1 Enterobacter cloacae y 1 Alcaligenes faecalis), isolated from adult patients with nosocomial pulmonary in...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract::We present here 6 patients with Fasciola hepatica infection, all from rural origin, and with the risk factors of eating wild watercress. The more common symptoms were: fever (83%), abdominal pain (100%), weight loss (83%) and generalized myalgia and joint pain (67%). All patients presented with an absolute eosinophil ...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract:BACKGROUND:The analysis of the immune response to rotavirus infection and the characterization of the viral antigens recognized by specific antibodies are of great concern in evaluating the protection against rotavirus. MATERIAL AND METHODS:The levels of rotavirus-specific fecal (sIgA) and serum antibodies (IgM, IgG a...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00