Abstract:
INTRODUCTION:The aim of this study was to know, through a national survey, the methods and techniques used for the diagnosis of Helicobacter pylori (Hp) in the different Clinical Microbiology Services/Laboratories in Spain, as well as antibiotic resistance data. METHODS:The survey requested information about the diagnostic methods performed for Hp detection in Clinical Microbiology laboratories, including serology, stool antigen, culture from gastric biopsies, and PCR. In addition, the performance of antibiotic susceptibility was collected. Data on the number of samples processed in 2016, positivity of each technique and resistance data were requested. The survey was sent by email (October-December 2017) to the heads of 198 Clinical Microbiology Laboratories in Spain. RESULTS:Overall, 51 centers from 29 regions answered the survey and 48/51 provided Hp microbiological diagnostic testing. Concerning the microbiological methods used to diagnose Hp infection, the culture of gastric biopsies was the most frequent (37/48), followed by stool antigen detection (35/48), serology (19/48) and biopsy PCR (5/48). Regarding antibiotic resistance, high resistance rates were observed, especially in metronidazole and clarithromycin (over 33%). CONCLUSION:Culture of gastric biopsies was the most frequent method for detection of Hp, but the immunochromatographic stool antigen test was the one with which the largest number of samples were analyzed. Nowadays, in Spain, it concerns the problem of increased antibiotic resistance to 'first-line' antibiotics.
journal_name
Enferm Infecc Microbiol Clinjournal_title
Enfermedades infecciosas y microbiologia clinicaauthors
Miqueleiz-Zapatero A,Alba-Rubio C,Domingo-García D,Cantón R,Gómez-García de la Pedrosa E,Aznar-Cano E,Leiva J,Montes M,Sánchez-Romero I,Rodríguez-Díaz JC,Alarcón-Cavero T,Grupo de Trabajo de Diagnóstico Microbiológico de Helicobactdoi
10.1016/j.eimc.2019.11.008subject
Has Abstractpub_date
2020-11-01 00:00:00pages
410-416issue
9eissn
0213-005Xissn
1578-1852pii
S0213-005X(19)30326-Xjournal_volume
38pub_type
杂志文章abstract:BACKGROUND:We evaluate two different commercially available kits for detecting specific antibodies against Helicobacter pylori in a group of 48 patients with gastrointestinal symptoms that underwent an endoscopic procedure. We also study the seroprevalence of anti-H. pylori antibodies in asimptomatic individuals from o...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract:INTRODUCTION:Nosocomial fungal infections have increased significantly in the last decade. Candida detection in clinical specimens can mean either colonization or an infection which can be local (muguet) or invasive. Knowledge of the species helps in choosing the best treatment. The aims of this study were to determine...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2012.09.011
更新日期:2013-06-01 00:00:00
abstract::Rilpivirine (RPV) is a new, second-generation nonnucleoside reverse transcriptase inhibitor (NNRTI) that has been recently approved for use in the initial antiretroviral therapy (ART) of treatment-naïve HIV-infected patients, combined with two nucleoside/nucleotide reverse transcriptase inhibitors (NRTI). The approved...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1016/S0213-005X(13)70139-3
更新日期:2013-06-01 00:00:00
abstract::We have evaluated the results of a serologic study of 34 patients with a possible chlamydial infection, obtaining three successive serum samples that were assessed with microimmunofluorescence (IIF) and complement fixation (CF) tests, so as to establish a comparison between the antibody titer measured by each of them....
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract:PURPOSE:Gordonia species are known to be opportunistic human pathogens causing secondary infections. We present the second case in the world of endocarditis caused by Gordonia bronchialis and a review of all the cases of endocarditis caused by Gordonia spp. METHODS:The identification was performed by matrix-assisted d...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2020.11.010
更新日期:2021-01-11 00:00:00
abstract:OBJECTIVES:To determine: (a) The prevalence of active infection by the hepatitis C virus (HCV) and hepatitis B virus (HBV) in HIV-infected patients, as well as previous exposure to hepatitis A virus (HAV), HBV and HCV. (b) The proportion of patients who have been vaccinated against HAV and/or HBV. (c) The HCV genotype ...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2011.12.008
更新日期:2012-10-01 00:00:00
abstract:INTRODUCTION:Identification of yeasts is based on morphological, biochemical and nutritional characteristics, and using molecular methods. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, a new method for the identification of microorganisms, has demonstrated to be very useful. ...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2014.10.003
更新日期:2015-06-01 00:00:00
abstract:BACKGROUND:The purpose of this work was to study the molecular basis of beta-lactamase production in ampicillin-resistant strains of Salmonella spp. METHODS:It was performed analytical isoelectric focusing of beta-lactamases produced by a group of 33 strains selected in basis of their resistance phenotype. Plasmid pro...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
abstract:AIMS:Francisella tularensis is a facultative intracellular gramnegative aerobic coccobacilli which is difficult to isolate. The appearance of an epidemic outbreak of tularemia in Castilla y Leon (Spain) during the first months of 1998 led bur microbiology laboratory to develop the diagnostic methods of this disease whi...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::The evolution of antimicrobial susceptibility of Bacteroides fragilis over a five year period is described. We have studied 30 selected strains isolated each year at the University Hospital of Sevilla (total: 150 strains). We did not find any resistant strain to chloramphenicol, metronidazole or imipenem. Resistance t...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
abstract:INTRODUCTION:Sustained virological response (SVR) 12 weeks after the end-of-therapy (EOT) has been correlated with SVR24 for HCV-monoinfection. We aim to validate SVR12 as criterion for definition of HCV cure in HIV-coinfected patients treated with all-oral direct-acting antivirals (DAA). METHODS:Prospectively observa...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2019.07.009
更新日期:2020-01-01 00:00:00
abstract::Prosthetic joint infection is an increasing public health problem. The bacterial biofilms that form on these foreign bodies are resistant to host defence mechanisms and antimicrobial therapy. Sixty per cent of prosthetic joint infections are caused by Staphylococcus sp., and they are usually acquired during surgery or...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1157/13123843
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:Infectious pharyngotonsillitis is usually managed with antibiotics by general practitioners and pediatricians both in primary care and the emergency services. In the present work we try to assess the antibiotic variability and appropriateness in the management of acute pharyngotonsillitis among several emerge...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to develop a satisfactory technique to detect punctual mutations in blaTEM genes. METHODS:The strains [E. coli HB 101 pBR322 (TEM-1), E. coli J62 RP4 (TEM-2)] were submitted to PCR with primers PL1 and PL2 which amplify the genetic region susceptible of punctual mutations. Then...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract:BACKGROUND:To determine the characteristics of HIV-infection in foreign patients residing in Elche, Spain. METHOD:A retrospective comparative analysis of clinical and epidemiological characteristics was performed to study HIV-infection in foreign immigrants, foreigners who were not immigrants and the native Spanish po...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1157/13078824
更新日期:2005-10-01 00:00:00
abstract:BACKGROUND:The mortality of severe tetanus (grades II-III) remains high today, being greater than 20% with a large number of complications, due to the lengthy stay of these patients in the ICU. METHODS:A review of 13 cases of severe tetanus over the last 5 years was performed analyzing age, entrance, previous immuniza...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:
更新日期:1997-05-01 00:00:00
abstract:INTRODUCTION:The use of protease inhibitors (PI) has led to a decrease in HIV-1-related mortality and morbidity. The objective of this study was to collect safety data on treatment with fosamprenavir/ritonavir (FPV/r) 700/100mg BID in HIV-infected patients through an expanded access program. PATIENTS AND METHODS:Prosp...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.eimc.2008.05.002
更新日期:2009-01-01 00:00:00
abstract:INTRODUCTION:The aim of this study was to analyze the distribution of human papillomavirus (HPV) genotypes in cytologically abnormal cervical samples from 106 women living in a region of the north of Spain. METHODS:Cytological classification was reported according to the 2001 Bethesda System and HPV genotyping was per...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2011.10.021
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The influenza A (H1N1) 2009 pandemic initially had a mild impact in Catalonian hospitals, but in the autumn there was an important pandemic wave. We describe the main characteristics of patients seen in the Vall d'Hebron University Hospital in Barcelona (HUVH) during this pandemic, the risk fac...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2011.05.005
更新日期:2011-10-01 00:00:00
abstract:BACKGROUND:Enteroviruses (EV) are the main aetiological agents of aseptic meningitis in children and a common cause of febrile illnesses in young infants in summer. A rapid diagnosis is essential to rule out other conditions. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay performed in cerebros...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2012.07.007
更新日期:2013-02-01 00:00:00
abstract:BACKGROUND:In recent years an increase has been observed in the prevalence of meningococcal infection by Neisseria meningitidis serogroup C and in the appearance of strains with moderate resistance to penicillin. PATIENTS AND METHODS:A microbiologic study of the cases of meningococcal infection of serogroup C treated ...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00
abstract::An outbreak of S.Typhimurium occurred in several towns and cities in the province of Castellon (Spain) between 23 February and 27 May 2011. On April 5, the microbiology laboratory of a hospital in Castellon alerted the health authorities to the increase in S.Typhimurium isolated in fecal culture of children with gastr...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2015.11.016
更新日期:2016-11-01 00:00:00
abstract::We retrospectively analyze the untoward effects due to cotrimoxazole (CMX) when this drug was used as initial therapy in 37 Spanish AIDS patients with Pneumocystis carinii pneumonia (PCP). A total of 46% of patients developed an adverse reaction, that was considered severe in 24.3% of cases, and prompted to stop CMX t...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1991-08-01 00:00:00
abstract::Epidemiological surveillance of HIV infection has made notably progress over the last decade, improving knowledge of its characteristics and adapting treatment accordingly. On the other hand, the delayed diagnosis of HIV infection is still an important problem to reduce transmission and improve epidemic control both n...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1016/j.eimc.2010.11.010
更新日期:2011-02-01 00:00:00
abstract:OBJECTIVE:In 1995 a specialized home care service for infectious diseases was created in our institution. The aim was to improve the quality of life of patients with prolonged parenteral antimicrobial therapy requirements, reduce the length of hospital stay, and improve the care received after discharge by clinical and...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1157/13108706
更新日期:2007-08-01 00:00:00
abstract:INTRODUCTION:the duration adequacy of antibiotic regimens is one of the key points of Antimicrobial Stewardship Programs (ASP) given the relationship between the risk of resistance and days of exposure to antimicrobials. METHODS:monitoring activities of intravenous antibiotics longer than 7 days at Hospital Infantil V...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2020.02.015
更新日期:2020-03-27 00:00:00
abstract::The prevalence of human immunodeficience virus (HIV) infection among patients under renal replacement therapy varies, with estimates of 1% for Europe and 1.5% for the United States. Survival in HIV infected individuals receiving renal replacement therapy has improved since the introduction of high activity antiretrovi...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1157/13076177
更新日期:2005-06-01 00:00:00
abstract::Quantification of hepatitis C virus (HCV) RNA (viral load) is the most widely used marker to diagnose and confirm active HCV infection. The HCV core antigen forms part of the internal structure of the virus and, like HCV RNA, its detection also indicates viral replication and presents certain advantages over viral loa...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2020.02.003
更新日期:2020-01-01 00:00:00
abstract::Multiresistant Gram-negative bacteria represent a major health problem worldwide. This is related to the severity of the infections they cause, the difficulties for empiric (even directed) treatment, the ease of multiresistance spread, and the absence of new antimicrobial agents active against this group of pathogens....
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1016/j.eimc.2013.03.016
更新日期:2013-06-01 00:00:00
abstract:INTRODUCTION:New tools are needed for early evaluation of patients who could be infected by COVID-19 during this pandemic. M-Health (apps) could be a solution in this setting to evaluate a COVID-19 diagnosis. The aim of this study was to describe which COVID-19s apps are available in Spain. METHODS:We made a review of...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2020.08.002
更新日期:2020-09-09 00:00:00