Abstract:
:Human proenkephalin generated by means of a recombinant vaccinia virus expression vector was used as the substrate for a putative processing enzyme obtained from bovine adrenal chromaffin granules. The adrenal enzyme successfully cleaved proenkephalin to generate low mol wt enkephalins as well as other enkephalin-containing intermediates. Radioactively labeled proenkephalin prepared with this system was also cleaved; however, under identical conditions bovine proinsulin was not cleaved. These results provide support for the notion that the adrenal trypsin-like enzyme is involved in the processing of proenkephalin in vivo and demonstrate the usefulness of protein substrates prepared by expression vector systems in testing the reactivity and specificity of proposed prohormone processing enzymes in vitro.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Lindberg I,Thomas Gdoi
10.1210/endo-126-1-480subject
Has Abstractpub_date
1990-01-01 00:00:00pages
480-7issue
1eissn
0013-7227issn
1945-7170journal_volume
126pub_type
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