Abstract:
:PhiC31 integrase has the potential to achieve long-term transgene expression because of site-specific and unidirectional recombination. In this study, plasmid DNA (pDNA) encoding Gaussia luciferase (Gluc) cDNA was constructed and the optimal condition for long-term gene expression using phiC31 integrase in mouse liver after hydrodynamic injection was investigated. Gluc is secreted and thus allows quantification of its expression level in blood and easy determination of the expression time-course. Mice were co-transfected with 25 µg donor pDNA (pORF-Gluc/attB) and different amounts of helper pDNA (pCMV-int; from 1 to 50 µg). Serum Gluc expression was evaluated during 120 d. The most highly sustained gene expression was obtained when 10 µg of helper pDNA was co-transfected in ICR and C57BL/6 mice. However, the Gluc expression in C57BL/6 mice was slightly lower and less durable than that in ICR mice. Little hepatic damage caused by phiC31 integrase was observed during 120 d in ICR and C57BL/6 mice.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Umemoto Y,Kawakami S,Otani Y,Higuchi Y,Yamashita F,Hashida Mdoi
10.1248/bpb.b12-00083subject
Has Abstractpub_date
2012-01-01 00:00:00pages
1182-6issue
7eissn
0918-6158issn
1347-5215pii
DN/JST.JSTAGE/bpb/b12-00083journal_volume
35pub_type
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