Nano-mechanical reinforcement in drug-resistant ovarian cancer cells.


:The mechanical properties of cells are considered promising biomarkers for the early detection of cancer and the testing of drug efficacy against it. Nevertheless, generalized correlations between drug resistance and the nano-mechanical properties of cancer cells are yet to be defined due to the lack of necessary studies. In this study, we conducted atomic force microscopy (AFM)-based nano-mechanical measurements of cisplatin-sensitive (A2780) and cisplatin-resistant (A2780cis) ovarian cancer cells. The difference in the efficacy of cisplatin between A2780 and A2780cis was confirmed in the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. We observed that the cisplatin-resistant ovarian cancer cells were more motile than cisplatin-sensitive cells based on the results of the wound closure experiment, and the AFM experiments showed that drug resistance induced nano-mechanical stiffening of the ovarian cancer cells. Increased mechanical stiffness caused by cisplatin resistance was consistent with the confocal microscopy images showing more distinct actin stress fibers in A2780cis than in A2780 cells. The down regulation of vinculin implicated the actin-driven elongation as a major motile mode for A2780cis cells. Our results consistently indicated that the acquisition of drug resistance in ovarian cancer cells induces an extensive reorganization of the actin cytoskeleton, which governs the cellular mechanical properties, motility, and possibly intracellular drug transportation.


Biol Pharm Bull


Seo YH,Jo YN,Oh YJ,Park S




Has Abstract


2015-01-01 00:00:00












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    authors: Yuasa H,Soga N,Kimura Y,Watanabe J

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    pub_type: 杂志文章


    authors: Wu ZQ,Guo QL,You QD,Zhao L,Gu HY

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    journal_title:Biological & pharmaceutical bulletin

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    pub_type: 杂志文章


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    pub_type: 杂志文章,评审


    authors: Higashi T,Mai Y,Mazaki Y,Horinouchi T,Miwa S

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


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    pub_type: 杂志文章


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    authors: Okada M,Imoto K,Sugiyama A,Yasuda J,Yamawaki H

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    pub_type: 杂志文章


    authors: Furuno K,Akasako T,Sugihara N

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  • Fas-mediated apoptosis is enhanced by glycyrrhizin without alteration of caspase-3-like activity.

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  • Anticytotoxin Effects of Amentoflavone to Pneumolysin.

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    authors: Zhao X,Liu B,Liu S,Wang L,Wang J

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    authors: Suzuki T,Suzuki Y

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    authors: Tanabe A,Kumahara C,Osada S,Nishihara T,Imagawa M

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    authors: Yanardağ R,Bolkent S,Tabakoğlu-Oğuz A,Ozsoy-Saçan O

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    pub_type: 杂志文章


    authors: Narazaki R,Maruyama T,Otagiri M

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    pub_type: 杂志文章


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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kanno S,Kitajima Y,Kakuta M,Osanai Y,Kurauchi K,Ujibe M,Ishikawa M

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Wang L,Zhang R,Chen J,Wu Q,Kuang Z

    更新日期:2017-04-01 00:00:00

  • Protective Effects of Curcumin on Manganese-Induced BV-2 Microglial Cell Death.

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章,评审


    authors: Takahashi M

    更新日期:2019-01-01 00:00:00