Abstract:
:Apoptotic cells must be recognized early for phagocytosis to ensure that their toxic contents do not damage neighboring cells. In some cases this is achieved via CD43-capped membrane glycoproteins, the sialylpolylactosaminyl chains of which serve as ligands for phagocytosis by macrophages. However, because many additional changes occur during apoptosis, determining exactly which events are responsible for signaling macrophages to initiate phagocytosis remains a challenge. Here, we examined one clearance mechanism in detail and determined that capping of CD43 alone is sufficient to initiate phagocytosis. We induced macrophage-mediated phagocytosis by using cytochalasin B to artificially cap CD43 on healthy (non-apoptotic) Jurkat cells. Additional experiments confirmed that sialylpolylactosaminyl chains formed through this capping method are a prerequisite for removal, and that nucleolin is the macrophage receptor responsible for their detection. These findings strongly suggest that capping of CD43 presents a sufficient signal for phagocytosis without any additional membrane changes.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Oguri E,Miki Y,Hirano K,Yamanaka M,Beppu Mdoi
10.1248/bpb.35.551subject
Has Abstractpub_date
2012-01-01 00:00:00pages
551-8issue
4eissn
0918-6158issn
1347-5215pii
JST.JSTAGE/bpb/35.551journal_volume
35pub_type
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