Cloning and characterization of Sdga gene encoding alpha-subunit of heterotrimeric guanosine 5'-triphosphate-binding protein complex in Scoparia dulcis.


:A homology-based cloning strategy yielded Sdga, a cDNA clone presumably encoding alpha-subunit of heterotrimeric guanosine 5'-triphosphate-binding protein complex, from leaf tissues of Scoparia dulcis. Phylogenetic tree analysis of G-protein alpha-subunits from various biological sources suggested that, unlike in animal cells, classification of Galpha-proteins into specific subfamilies could not be applicable to the proteins from higher plants. Restriction digests of genomic DNA of S. dulcis showed a single hybridized signal in Southern blot analysis, suggesting that Sdga is a sole gene encoding Galpha-subunit in this plant. The expression level of Sdga appeared to be maintained at almost constant level after exposure of the leaves to methyl jasmonate as analyzed by reverse-transcription polymerase chain reaction. These results suggest that Sdga plays roles in methyl jasmonate-induced responses of S. dulcis without a notable change in the transcriptional level.


Biol Pharm Bull


Shite M,Yamamura Y,Hayashi T,Kurosaki F




Has Abstract


2008-11-01 00:00:00














  • Occurrence of a novel L-2,4-diaminobutyrate decarboxylase activity in some species of Enterobacteriaceae, and purification and characterization of the enzymes of Enterobacter aerogenes and Serratia marcescens.

    abstract::L-2,4-Diaminobutyrate decarboxylase (DABA DC) is a novel enzyme yielding 1,3-diaminopropane (DAP) from DABA, which has previously been purified from strains of the genera Vibrio and Acinetobacter. In this study, we also detected DABA DC activity in the species of Enterobacteriaceae: E. aerogenes, E. cloacae, E. agglom...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yamamoto S,Mutoh N,Ikai H,Nagasaka M

    更新日期:1996-10-01 00:00:00

  • Anticonvulsant activity of paeonimetabolin-I adducts obtained by incubation of paeoniflorin and thiol compounds with Lactobacillus brevis.

    abstract::Seventeen thiopaeonimetabolin-I adducts were obtained as mixtures of diastereoisomers after incubation of paeoniflorin with Lactobacillus brevis in the presence of various thiols. The anticonvulsant activity of the adducts was investigated in mice using the maximal subcutaneous pentylenetetrazol seizure test and sodiu...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Abdel-Hafez AA,Meselhy MR,Nakamura N,Hattori M,Watanabe H,Murakami Y,El-Gendy MA,Mahfouz NM,Mohamed TA

    更新日期:1999-05-01 00:00:00

  • In vitro study of the adsorption characteristics of drugs.

    abstract::The adsorption characteristics of eight adsorbents, cholestyramine, colestimide, aluminum silicate, sucralfate, aluminum hydroxide, calcium polystyrene sulfonate, carbon sphere and medicinal carbon, on the drugs such as methotrexate, antidepressants, mizoribine and ciprofloxacin hydrochloride were investigated in vitr...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Toyoguchi T,Ebihara M,Ojima F,Hosoya J,Nakagawa Y

    更新日期:2005-05-01 00:00:00

  • Antiinflammatory effects of orientin-2"-O-galactopyranoside on lipopolysaccharide-stimulated microglia.

    abstract::Microglia activation-mediated neuroinflammation plays an important role in the pathogenesis of neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, multiple sclerosis, and human immunodeficiency virus (HIV)-associated dementia. Inhibition of microglia activation may alleviate neurodegeneration ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Zhou X,Gan P,Hao L,Tao L,Jia J,Gao B,Liu JY,Zheng LT,Zhen X

    更新日期:2014-01-01 00:00:00

  • Expression patterns of plasma proteins in spontaneously diabetic rats after oral administration of a Kampo medicine, Hachimi-jio-gan, using SELDI ProteinChip platform.

    abstract::We investigated the changes (increase or decrease in peak intensity) in the expression of plasma proteins in spontaneously diabetic WBN/Kob rats that were with complicated diabetic nephropathy, to determine multiple biomarkers in the plasma of diabetic rats. The present study using surface enhanced laser desorption/io...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kiga C,Nakagawa T,Koizumi K,Sakurai H,Shibagaki Y,Ogawa K,Goto H,Saiki I

    更新日期:2005-06-01 00:00:00

  • Pharmacokinetic study on the multi-constituents of Huangqin-Tang decoction in rats.

    abstract::Using a validated high-performance liquid chromatographic (HPLC) method, the pharmacokinetics of multi-constituents in Huangqin-Tang decoction were simultaneously studied both in the compound prescription and in each single herb decoction. At different intervals (0, 1, 2, 4, 8, 12, 24, 36, 48 h) after oral administrat...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Zuo F,Zhou ZM,Zhang Q,Mao D,Xiong YL,Wang YL,Yan MZ,Liu ML

    更新日期:2003-07-01 00:00:00

  • Characterization of an apoptosis-inducing factor in Habu snake venom as a glycyrrhizin (GL)-binding protein potently inhibited by GL in vitro.

    abstract::By means of successive heparin-affinity and glycyrrhizin (GL)-affinity column chromatographies (HPLC), a 55 kDa GL-binding protein (gp55) was purified to apparent homogeneity from the Superdex P-I fraction of Habu snake venom. This gp55 was identified as an apoxin I-like protein, because (i) its 20 N-terminal amino ac...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Abe Y,Shimoyama Y,Munakata H,Ito J,Nagata N,Ohtsuki K

    更新日期:1998-09-01 00:00:00

  • Deoxynybomycin is a selective anti-tumor agent inducing apoptosis and inhibiting topoisomerase I.

    abstract::Deoxynybomycin was identified as an inducer of p21the/WAF1 gene following screening using a reporter, p21/luciferase. The present study examined its anti-proliferative effect on human tumor cell lines. Deoxynybomycin selectively inhibited growth of human osteoblastic sarcoma Saos-2, gastric cancer TMK-1, and monocytic...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Egawa K,Yamori T,Nosaka C,Kunimoto S,Takeuchi T,Nos K

    更新日期:2000-09-01 00:00:00

  • Targeting the extracellular signal-regulated kinase pathway in cancer therapy.

    abstract::The extracellular signal-regulated kinase (ERK) pathway is a major determinant in the control of diverse cellular processes such as proliferation, survival, and motility. This pathway is often upregulated in human cancers and as such represents an attractive target for mechanism-based approaches to cancer treatment. H...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章,评审


    authors: Kohno M,Tanimura S,Ozaki K

    更新日期:2011-01-01 00:00:00

  • Long term administration of Hypericum perforatum improves spatial learning and memory in the water maze.

    abstract::The aim of the present study is to investigate the effects of long-term Hypericum perforatum treatment on spatial learning and memory in rats. Hypericum preparation (HP) standardized to 0.3% hypericin content was administered orally for 9 weeks in doses of 4.3 and 13 microg/kg corresponding to therapeutic dosages in h...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Widy-Tyszkiewicz E,Piechal A,Joniec I,Blecharz-Klin K

    更新日期:2002-10-01 00:00:00

  • Lavandulyl flavonoids from Sophora flavescens suppress lipopolysaccharide-induced activation of nuclear factor-kappaB and mitogen-activated protein kinases in RAW264.7 cells.

    abstract::Oxidized low-density lipoprotein (oxLDL) and reactive oxygen species (ROS) play key roles in the early stage of atherosclerosis. Nitric oxide (NO) and ROS are responsible for regulation of the transcriptional pathways of nuclear Factor-kappaB (NF-kappaB) and mitogen-activated protein kinase (MAPK), key regulators of c...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Han JM,Jin YY,Kim HY,Park KH,Lee WS,Jeong TS

    更新日期:2010-01-01 00:00:00

  • The GCR2 gene is required for the transcriptional activation of retrotransposon Ty2-917 in Saccharomyces cerevisiae.

    abstract::Ty2 retrotransposons are the mobile genetic elements of the yeast Saccharomyces cerevisiae. Transcriptional regulation of Ty2-917 requires a complex set of cis acting elements which are located both upstream and downstream of the transcription initiation site. Previously, the glycolysis regulatory protein Gcr1p has be...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Türkel S

    更新日期:2002-09-01 00:00:00

  • Phenolic constituents of galla Rhois with hepatoprotective effects on tacrine- and nitrofurantoin-induced cytotoxicity in Hep G2 cells.

    abstract::The bioassay-guided fractionation of the MeOH extract of Galla Rhois furnished two hepatoprotective compounds, an equilibrium mixture of 3-galloyl-gallic acid and 4-galloyl-gallic acid isomers (3), 1,2,3,4,6-penta-O-galloyl-beta-D-glucose (4), and two inactive phenolic compounds, gallic acid methyl ester (1) and galli...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: An RB,Oh H,Kim YC

    更新日期:2005-11-01 00:00:00

  • Regulation of Octn2 transporter (SLC22A5) by peroxisome proliferator activated receptor alpha.

    abstract::The tissue distribution and disposition of carnitine, which plays an important role in the transport of long-chain fatty acids across the mitochondrial inner membrane for beta-oxidation, are well controlled by carnitine transporter organic cation/carnitine transporter 2 (OCTN2). Since little information is available o...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Maeda T,Wakasawa T,Funabashi M,Fukushi A,Fujita M,Motojima K,Tamai I

    更新日期:2008-06-01 00:00:00

  • Dysregulation of BSEP and MRP2 May Play an Important Role in Isoniazid-Induced Liver Injury via the SIRT1/FXR Pathway in Rats and HepG2 Cells.

    abstract::To explore the role of the abnormal expression of the bile salt export pump (BSEP) and multidrug resistance protein 2 (MRP2) in isoniazid (INH)-induced liver injury, we assessed the liver injury induced by INH in rats and HepG2 cells in vitro. The regulatory pathways via Sirtuin 1 (SIRT1) and farnesoid X receptor (FXR...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Qu X,Zhang Y,Zhang S,Zhai J,Gao H,Tao L,Song Y

    更新日期:2018-01-01 00:00:00

  • Influence of thermodynamic parameter in Lanosterol 14alpha-demethylase inhibitory activity as antifungal agents: a QSAR approach.

    abstract::A quantitative structure activity relationship, Hansch approach was applied on twenty compounds of chromene derivatives as Lanosterol 14alpha-demethylase inhibitory activity against eight fungal organisms. Various physicochemical descriptors and reported minimum inhibitory concentration values of different fungal orga...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Vasanthanathan P,Lakshmi M,Arockia Babu M,Kaskhedikar SG

    更新日期:2006-06-01 00:00:00

  • Psychiatric Patients with Antipsychotic Drug-Induced Hyperprolactinemia and Menstruation Disorders.

    abstract::Treatment with antipsychotic drugs has been associated with hyperprolactinemia. The same antipsychotic drugs have also been associated with side effects such as menstruation disorders. The aim of this study was to evaluate the prevalence of hyperprolactinemia and menstruation disorders in women undergoing antipsychoti...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Takechi K,Yoshioka Y,Kawazoe H,Tanaka M,Takatori S,Kobayashi M,Matsuoka I,Yanagawa H,Zamami Y,Imanishi M,Ishizawa K,Tanaka A,Araki H

    更新日期:2017-01-01 00:00:00

  • Extract from Nandina domestica inhibits lipopolysaccharide-induced cyclooxygenase-2 expression in human pulmonary epithelial A549 cells.

    abstract::Extract from fruits of Nandina domestica THUNBERG (NDE) has been used to improve cough and breathing difficulty in Japan for many years. To explore whether NDE may alleviate respiratory inflammation, we investigated its effect on expression of cyclooxygenase-2 (COX-2) and production of prostaglandin E₂ (PGE₂) in human...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ueki T,Akaishi T,Okumura H,Abe K

    更新日期:2012-01-01 00:00:00

  • O/W lipid emulsions for parenteral drug delivery. I. Pharmacokinetics of the oil particles and incorporated sudan II.

    abstract::The potential usefulness of oil in water (O/W) lipid emulsions as parenteral drug delivery system for lipophilic drugs was examined in tumor-bearing rats. A model lipophilic drug, sudan II (PCoct = 226000), was formulated in five lipid emulsions consisting of soybean oil and various surfactants. Compared with HCO-60 m...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Sakaeda T,Takahashi K,Nishihara Y,Hirano K

    更新日期:1994-11-01 00:00:00

  • Clonazepam use for prevention of acute and delayed vomiting induced by Cisplatin-based chemotherapy for lung cancer.

    abstract::We investigated the efficacy of clonazepam as an antiemetic in cisplatin-based chemotherapy for lung cancer. Seven patients experienced cisplatin-induced vomiting despite antiemetic therapy including 5-hydroxytryptamine(3) (5-HT(3)) antagonist and dexamethasone. Therefore, the antiemetic therapy including clonazepam, ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 临床试验,杂志文章


    authors: Hayashi M,Takao Y,Hata C,Teramachi H,Tsuchiya T

    更新日期:2010-01-01 00:00:00

  • Hydrocortisone inhibits cellular proliferation by downregulating hepatocyte growth factor synthesis in human osteoblasts.

    abstract::Glucocorticoids have multiple systemic effects that may influence bone metabolism but also directly affect osteoblasts by decreasing their proliferation. Using human osteoblastic SaM-1 cells, we examined whether the effects of hydrocortisone on cellular proliferation are mediated by hepatocyte growth factor (HGF). Hum...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Tsunashima Y,Kondo A,Matsuda T,Togari A

    更新日期:2011-01-01 00:00:00

  • Antihypertensive actions of methylripariochromene A from Orthosiphon aristatus, an Indonesian traditional medicinal plant.

    abstract::Methylripariochromene A (MRC) was isolated from the leaves of Orthosiphon aristatus (Lamiaceae) and subjected to the examination of several pharmacological actions related to antihypertensive activity. The following four findings were revealed from the present study: 1) MRC caused a continuous decrease in systolic blo...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Matsubara T,Bohgaki T,Watarai M,Suzuki H,Ohashi K,Shibuya H

    更新日期:1999-10-01 00:00:00

  • Osthole Alleviates Bleomycin-Induced Pulmonary Fibrosis via Modulating Angiotensin-Converting Enzyme 2/Angiotensin-(1-7) Axis and Decreasing Inflammation Responses in Rats.

    abstract::Studies have shown that angiotensin-converting enzyme 2 (ACE2) plays modulating roles in lung pathophysiology, including pulmonary fibrosis (PF) and acute lung injury. Pulmonary fibrosis is a common complication in these interstitial lung diseases, and PF always has a poor prognosis and short survival. To date, there ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Hao Y,Liu Y

    更新日期:2016-01-01 00:00:00

  • The substrate binding preferences of Plasmodium thymidylate kinase.

    abstract::Plasmodium falciparum thymidylate kinase (PfTMK) is a potential chemotherapeutic target as it can tolerate a range of substrates, which distinguishes it from other thymidylate kinases. An important step in drug development is to determine the interaction of ligands competing for their target sites in a proposed drug t...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kandeel M,Kitade Y

    更新日期:2011-01-01 00:00:00

  • Telekin induces apoptosis associated with the mitochondria-mediated pathway in human hepatocellular carcinoma cells.

    abstract::Telekin, a eudesmane-type sesquiterpene lactone compound isolated from Chinese folk medicine Carpesium divaricatum, has been reported to strongly inhibit the proliferation of cancer cells. In this study, the involvement of a mitochondria-mediated pathway in the pro-apoptotic action of telekin was investigated in human...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Zheng B,Wu L,Ma L,Liu S,Li L,Xie W,Li X

    更新日期:2013-01-01 00:00:00

  • Relationship between lipophilicity and skin permeability of various drugs from an ethanol/water/lauric acid system.

    abstract::The in vitro skin permeability of 16 drugs with a wide range of lipophilicity (log P; -0.95-4.40) was evaluated by the use of an ethanol/water (60/40) binary vehicle with or without lauric acid as a permeation enhancer. The enhancing effect by the addition of lauric acid to the ethanol/water (60/40) binary vehicle cou...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Lee CK,Uchida T,Kitagawa K,Yagi A,Kim NS,Goto S

    更新日期:1994-10-01 00:00:00

  • Investigation of phagocyte-inducible factor in tumor-implanted mice.

    abstract::The number of spleen Mac-1-positive cells was markedly increased in methylcholanthrene-induced Meth-A fibrosarcoma (Meth-A)-implanted mice. When the sera of Meth-A-implanted mice were added to normal bone marrow cells, granulocyte and macrophage colony stimulating factor (GM-CSF)-dependent growth of bone marrow cells ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Watanabe T,Okada A,Mikami T,Matsumoto T,Suzuki M

    更新日期:1995-12-01 00:00:00

  • Metabolic changes in paraquat poisoned patients and support vector machine model of discrimination.

    abstract::Numerous people die of paraquat (PQ) poisoning every year in the world. Although several studies regarding paraquat (PQ) poisoning have been conducted, the metabolic changes in plasma remain unknown. In this study, the metabolomics of 15 PQ poisoned patients with plasma PQ concentrations in excess of 0.1 µg/mL and 16 ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Wang X,Zhang M,Ma J,Zhang Y,Hong G,Sun F,Lin G,Hu L

    更新日期:2015-01-01 00:00:00

  • Studies on pharmacological activation of human immunoglobulin G by chemical modification and active subfragments. XIII. Effect of carboxamidemethylated Fc fragment (CM-Fc) on type II collagen-induced arthritis in DBA/1J mice.

    abstract::The effect of carboxamidemethylated Fc fragment (CM-Fc) from human immunoglobulin G (IgG) on type II collagen (C II)-induced arthritis (CIA) in DBA/1J mice was studied. CM-Fc suppressed the development of CIA while Fc fragment did not. CM-Fc inhibited the increase in serum anti-C II IgG. CM-Fc suppressed C II-induced ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Mimura T,Itoh M,Gohgi H,Suzuki N,Takeuchi K,Tsujikawa K,Kohama Y

    更新日期:1994-05-01 00:00:00

  • Effects of Angiotensin II Receptor Blockers on Metabolism of Arachidonic Acid via CYP2C8.

    abstract::Arachidonic acid (AA) is metabolized to epoxyeicosatrienoic acids (EETs) via cytochrome enzymes such as CYP 2C9, 2C8 and 2J2. EETs play a role in cardioprotection and regulation of blood pressure. Recently, adverse reactions such as sudden heart attack and fatal myocardial infarction were reported among patients takin...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Senda A,Mukai Y,Toda T,Hayakawa T,Yamashita M,Eliasson E,Rane A,Inotsume N

    更新日期:2015-01-01 00:00:00