Abstract：
BACKGROUND:Mechanical transduction contributes to appropriate cell functions. Clinically, keloid, an uncontrolled fibrous overgrowth and scarring, preferentially affects skin areas subject to higher mechanical tension than others. Keloid-derived fibroblasts have exaggerated TGF-beta1-mediated responses, including smooth muscle actin (SMA) expression, cellular contraction, and tissue remodeling, to mechanical strain compared to normal fibroblasts. OBJECTIVE:This study asked if SMA contributes to cellular intrinsic rigidity using keloid -derived fibroblasts as a model. METHOD:Using atomic force microscopy and confocal microscopy, we measured cellular rigidity and the expression of SMA in keloid fibroblasts treated with exogenous TGF-beta1. RESULT:There was an increase of SMA expression in keloid tissue as well as keloid-derived fibroblasts. The cell rigidity increased by TGF-beta1 in keloid fibroblasts occurred concomitantly with increases in SMA expression. TGF-beta1 receptor 1 kinase inhibitors reduced TGF-beta1-induced cellular rigidity and SMA expression. Knocking down SMA with interference RNA resulted in a reduction of TGF-beta1-enhanced rigidity, suggesting that TGF-beta1 increases cell rigidity via SMA expression. CONCLUSION:We conclude that TGF-beta1 increases cell rigidity through TGF-beta1 receptor-SMA axis. This study reports that SMA, at least in part, contributes to cell rigidity in fibroblasts. SMA might be an appealing pharmaceutical target in keloids.
journal_name
J Dermatol Scijournal_title
Journal of dermatological scienceauthors
Lee CH,Hong CH,Chen YT,Chen YC,Shen MRdoi
10.1016/j.jdermsci.2012.06.004subject
Has Abstractpub_date
2012-09-01 00:00:00pages
173-80issue
3eissn
0923-1811issn
1873-569Xpii
S0923-1811(12)00193-4journal_volume
67pub_type
杂志文章abstract：:Surgically resected specimens of skin appendage tumors, spiradenoma, poroma of eccrine glands, syringoadenoma, syringoadenoma papilliferum and mixec tumor of skin were evaluated for tenascin, as well as the basement membrane-associated protein laminin, and the extracellular matrix protein fibronectin, using immunohist...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/0923-1811(94)00369-p
更新日期：1995-03-01 00:00:00
abstract：BACKGROUND:Decreased skin elasticity is considered as a factor that promotes wrinkle formation. However, the relationship between decreased skin elasticity and the formation of wrinkles is not fully understood. OBJECTIVE:The purpose of this study is to characterize the relationship between skin elasticity and wrinkle ...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2007.05.002
更新日期：2007-09-01 00:00:00
abstract：:Mast cells are not only the primary effector cells of immediate type immune reactions, but they have recently also been considered to contribute to the induction of an immune response. Data on the ability of the cells to express major histocompatibility complex (MHC) antigens and the mechanisms involved are however co...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/s0923-1811(97)00033-9
更新日期：1997-11-01 00:00:00
abstract：:We previously showed that 12-O-tetradecanoylphorbol-13-acetate (TPA) and Ca2+-switch from low (0.07 mM) to normal (1.87 mM) concentration in culture medium, which were also linked to activation of protein kinase C (PKC), lead to phosphorylation of 180 kDa-bullous pemphigoid antigen (BPAG) 2, but not of 230 kDa-BPAG1, ...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/s0923-1811(01)00137-2
更新日期：2001-11-01 00:00:00
abstract：BACKGROUND:It is well known that cyclophosphamide (Cy) treatment before sensitization paradoxically enhances rather than suppresses contact hypersensitivity (CH) reactions. In fact, Cy-treated mice developed a significant (p < 0.05) increase of the CH reactions to 2,4,6-trinitro-1-chrolobenzene (TNCB) in comparison wit...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2005.02.002
更新日期：2005-08-01 00:00:00
abstract：:We investigated the adherence characteristics of Staphylococcus aureus and coagulase-negative staphylococci (CNS) isolated from various skin lesions by examining hydrophobicity, negative charge, tube adherence, slime production, and promotion of adherence to coverslips by blood proteins. Our results in the present stu...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/s0923-1811(98)00036-x
更新日期：1998-11-01 00:00:00
abstract：BACKGROUND:Skin lesions commonly affect AIDS patients. The pathogenesis of certain dermatologic disorders primarily associated to HIV-1 is unclear, and better forms of therapy for these conditions need to be discovered. Transgenic animal models represent a novel approach for the study of these disorders and for the que...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2008.08.015
更新日期：2009-02-01 00:00:00
abstract：:Skin sulfhydryl oxidase (SOx) is an enzyme that catalyzes disulfide (S-S) cross-linking through the oxidation of sulfhydryl compounds in the skin. In this study, using the enzyme purified from rat seminal vesicle, we obtained peptide sequences for SOx by mass spectrometry. We then searched for SOx nucleotides correspo...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/s0923-1811(02)00061-0
更新日期：2002-10-01 00:00:00
abstract：BACKGROUND:Transforming growth factor beta 1 (TGFβ) is known to be a regulator of autoimmunity. Loss of TGFβ leads to severe multi-organ autoimmunity in mice. In skin, role of TGFβ in suppressing autoimmunity is unclear. OBJECTIVE:Determine whether Keratinocyte (KC)-derived TGFβ is required for skin immune homeostasis...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2019.04.008
更新日期：2019-05-01 00:00:00
abstract：BACKGROUND:Histamine 4 receptor (H4R) antagonists are considered as new therapeutics for the treatment of atopic dermatitis (AD) and first clinical trials have already shown promising results. Histamine 1 receptor (H1R) antagonists are traditionally used to treat AD although the evidence for the efficacy is weak. The c...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2017.04.004
更新日期：2017-08-01 00:00:00
abstract：:Using fetal rat keratinizing epidermal cells (FRSK cells) we investigated the effects of retinoids on the cyclic AMP system. The beta-adrenergic adenylate cyclase response was significantly decreased by the treatment of cells with 1 x 10(-6) M Ro 10-1670. The effect was observed by 12 h and remained for at least 48 h....
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/0923-1811(93)90080-9
更新日期：1993-04-01 00:00:00
abstract：BACKGROUND:Zinc deficiency is believed to be a predisposing factor for the development and intractable healing of pressure ulcers (PUs); however, the mechanisms of this association have not been elucidated. OBJECTIVE:Objective was to elucidate the mechanisms of the formation of severe and prolonged PUs under the zinc ...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2019.07.004
更新日期：2019-08-01 00:00:00
abstract：:Crystalloid inclusion bodies (CIB) of the endothelial cells (EC) were investigated in blood vessels of human fetal skin and the umbilical cord by electron- and immunoelectron microscopy. They were found in up to 15% of the investigated EC in various types of vessels. Their sizes ranged from 0.2 microns to 0.6 microns ...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/0923-1811(91)90020-x
更新日期：1991-03-01 00:00:00
abstract：BACKGROUND:Atopic dermatitis (AD) is characterized by impaired skin barrier function and immune system dysfunction. The expression and role of Yes-associated protein (YAP) in AD are unclear. OBJECTIVE:To characterize the role of the YAP in T cell imbalance and epidermal keratinocyte dysfunction in the pathogenesis of ...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2020.12.004
更新日期：2020-12-18 00:00:00
abstract：BACKGROUND:Pseudoxanthoma elasticum (PXE) is an inherited systemic disorder, characterized by dermal, ocular and cardiovascular lesions. Genetic defects of the ABCC6 (MRP6) transporter are known to cause PXE. OBJECTIVES:The purpose of this study was to identify the genetic background of a PXE patient with a very early...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2005.07.010
更新日期：2005-11-01 00:00:00
abstract：:Cyclosporin A (CsA), a cyclic endecapeptide, is a T cell-specific immunosuppressant and is successfully used in the field of organ transplantation. Another T cell-specific immunosuppressant, FK506, a more recently discovered macrolide antibiotic, is effective against graft rejection at much lower doses than CsA. Altho...
journal_title：Journal of dermatological science
pub_type： 杂志文章,评审
doi：10.1016/0923-1811(94)90035-3
更新日期：1994-07-01 00:00:00
abstract：:The unfolded protein response (UPR) is a signaling pathway from the endoplasmic reticulum (ER) to the nucleus that protects cells from stress caused by misfolded or unfolded proteins. As such, ER stress is an ongoing challenge for all cells, given the central biologic importance of secretion as part of normal physiolo...
journal_title：Journal of dermatological science
pub_type： 杂志文章,评审
doi：10.1016/j.jdermsci.2012.12.002
更新日期：2013-03-01 00:00:00
abstract：:The epidermal basement membrane zone (BMZ) comprises a multiprotein complex that aids the attachment of epidermal keratinocytes to the underlying dermis. In the last 5 years, our understanding of epidermal BMZ morphology, structure and function has dramatically improved. A complex network of molecular interactions has...
journal_title：Journal of dermatological science
pub_type： 杂志文章,评审
doi：10.1016/s0923-1811(03)00045-8
更新日期：2003-05-01 00:00:00
abstract：BACKGROUND:The appearance and function of human skin are dramatically altered with aging, resulting in higher rates of severe xerosis and other skin complaints. The outermost layer of the epidermis, the stratum corneum (SC), is responsible for the biomechanical barrier function of skin and is also adversely transformed...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2015.07.016
更新日期：2015-11-01 00:00:00
abstract：:Hair follicles reconstitute themselves though the hair cycle, suggesting the presence of intrinsic stem cells. In contrast to the previous belief that stem cells reside in the bulbar region of hair follicles, stem cells were detected in the bulge area, a contiguous part of outer root sheath, that provides the insertio...
journal_title：Journal of dermatological science
pub_type： 杂志文章,评审
doi：10.1016/j.jdermsci.2006.12.002
更新日期：2007-05-01 00:00:00
abstract：:Propionibacterium acnes (P. acnes) causes an inflammatory acne that is characterized by massive neutrophilic infiltration. IL-8 is thought to play an important role in the pathophysiology of P. acnes, although the mechanisms by which P. acnes up-regulates the release of IL-8, a neutrophilic chemokine, from target cell...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/s0923-1811(02)00013-0
更新日期：2002-08-01 00:00:00
abstract：:Quinolone antibacterial agents, known to elicit photosensitive dermatitis as an adverse effect, have both phototoxicity and photoallergenicity. The latter potency is mainly derived from their photohaptenic moiety; quinolones covalently bind to protein and cells upon exposure to ultraviolet A (UVA) light. Our previous ...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/s0923-1811(99)00011-0
更新日期：1999-09-01 00:00:00
abstract：BACKGROUND:NIPAL4, encoding the NIPA-like domain containing 4 protein (NIPAL4), is one of the causative genes of autosomal recessive congenital ichthyosis (ARCI). The physiological role of NIPAL4 and the pathogenetic mechanisms of ARCI caused by NIPAL4 mutations remain unclear. OBJECTIVE:To clarify the changes of cera...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2019.12.001
更新日期：2020-01-01 00:00:00
abstract：:Matrix metalloproteinases (MMPs) play an important role in tumor invasion and metastasis. Enhanced expression of matrix metalloproteinase-2 (MMP-2) has been demonstrated in dermatofibroma (DF) and malignant fibrous histiocytoma (MFH). MMP-2 has been shown to be activated by membrane-type MMPs (MT-MMPs). To study the r...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/s0923-1811(01)00159-1
更新日期：2002-02-01 00:00:00
abstract：BACKGROUND:Using JAK inhibitors to inhibit cytokine signaling is presumed to be a possible means of treating skin inflammatory disorders such as contact dermatitis. OBJECTIVE:To clarify the action site of JAK inhibitors in skin inflammatory disorders. METHODS:We analyzed the mechanism of action of the JAK inhibitor J...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2016.09.007
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND:Currently available topical treatments for actinic keratosis (AK) are associated with substantial side-effects. OBJECTIVES:To evaluate the efficacy and safety of topical SR-T100 gel in treating AK. METHODS:A multicenter, randomized, double-blinded phase III trial was conducted. Patients with at least two c...
journal_title：Journal of dermatological science
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.jdermsci.2018.02.015
更新日期：2018-06-01 00:00:00
abstract：:Etiology of connective tissue disease is unknown. The association of infectious agents has been suspected serologically, ultrastructurally and recently by means of molecular biological techniques. We extracted RNA from lesions of 25 discoid lupus erythematosus, 9 systemic LE, and 3 systemic sclerosis biopsies, and rev...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/0923-1811(95)00422-x
更新日期：1996-01-01 00:00:00
abstract：:An immunoperoxidase technique was performed to detect keratinolytic proteinase (KPase) in sections of the skin taken from guinea pigs infected with Microsporum canis and in cultured M. canis using polyclonal antisera to purified KPase. Of tissue samples from guinea pigs infected with M. canis, all sections of erythema...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/0923-1811(90)90015-6
更新日期：1990-11-01 00:00:00
abstract：BACKGROUND:Retinoic acid (RA) enhances skin-lightening capabilities of hydroquinone (HQ), at least in part, by facilitating desquamation which leads to increase penetration of HQ. The desquamation also affects skin irritation levels. The mechanism of RA-induced desquamation, however, has not been completely explored an...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/j.jdermsci.2017.03.023
更新日期：2017-08-01 00:00:00
abstract：:We succeeded in culturing human outer root sheath cells (ORSC) in serum-free conditions without a biological feeder layer. The combination of collagen type IV substrate and modified MCDB 153 medium supplemented with bovine pituitary gland extract (K-GM medium) could support the growth of ORSC. These cells can be seria...
journal_title：Journal of dermatological science
pub_type： 杂志文章
doi：10.1016/0923-1811(91)90045-y
更新日期：1991-01-01 00:00:00
