Abstract：
:Glucocorticoids (GC) represent the main treatment for pemphigus; however, some patients show GC resistance. GC sensitivity was evaluated in 19 pemphigus patients and 41 controls by the number of binding sites [B(max) (fmol/mg protein)] and the affinity of GC receptor [Kd (nM)] to dexamethasone (DEX) as well as by the pattern of cytokine by DEX-mediated inhibition of concanavalin-A (Con-A)-stimulated PBMC proliferation. The Kd (15.7 ± 2.8 vs.8.1 ± 1.3) and Bmax (6.5 ± 0.9 vs. 3.9 ± 0.3) were higher in pemphigus than controls (p = 0.002). Considering the values above the 95th percentile of normal group as a cut-off (K(d) > 24.9 nM and B(max) > 8.1 fmol/mg protein), elevated K(d) and B(max) were observed in 9.8% and 2.4% of controls and 15.8% and 36.8% of patients (p = 0.02). PBMC proliferation was stimulated by Con-A and inhibited by DEX (p < 0.001) in both pemphigus and control groups. IL-6 and TNFα (pg/mL) basal production were higher in patients than controls. There was an increment of these cytokines after Con-A stimulation, and they were inhibited by DEX (p = 0.002) in controls and remained elevated in pemphigus (p < 0.02). Patients and controls showed no difference in basal and stimulated production of IL-8 and IL-10. There is an alteration on GC sensitivity in pemphigus patients and a higher production of proinflammatory cytokines. Therefore, in pemphigus patients, proinflammatory cytokines might be involved in the mechanism of GC resistance and/or in its maintenance.
journal_name
J Clin Immunoljournal_title
Journal of clinical immunologyauthors
Chriguer RS,Roselino AM,de Castro Mdoi
10.1007/s10875-012-9679-ysubject
Has Abstractpub_date
2012-08-01 00:00:00pages
786-93issue
4eissn
0271-9142issn
1573-2592journal_volume
32pub_type
杂志文章abstract：PURPOSE:Sarcoidosis is a non-caseating granulomatous disease for which a role for infectious antigens continues to strengthen. Recent studies have reported molecular evidence of mycobacteria or propionibacteria. We assessed for immune responses against mycobacterial and propionibacterial antigens in sarcoidosis broncho...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/s10875-012-9700-5
更新日期：2012-10-01 00:00:00
abstract：PURPOSE:T-helper (Th) cells abnormalities are considered to be associated with the pathogenesis of Systemic lupus erythematosus (SLE). Recently, The Th22 cells have been identified and implicated in the pathogenesis of autoimmune diseases such as Rheumatoid arthritis (RA), although therir role in Systemic lupus erythem...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/s10875-013-9878-1
更新日期：2013-05-01 00:00:00
abstract：:Cytotoxic lymphocytes are critical in the control of HIV replication, it has been shown that perforin is the key effector of killing machinery for CTLs and NK cells, so we investigated the circulating levels of perforin in CD8+ T cells and NK cells by flow cytometry intracellular stain in Chinese HIV infected individu...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/s10875-006-9027-1
更新日期：2006-07-01 00:00:00
abstract：:Two hundred twelve patients with enzyme immunoassay and Western blot confirmation of human immunodeficiency virus (HIV) infection were evaluated with anergy panel, lymphocyte cell surface phenotyping, lymphocyte transformation, and serum immunoglobulins. Mitogen responses were used to develop a lymphocyte transformati...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/BF00916939
更新日期：1989-03-01 00:00:00
abstract：:A molecular analysis of complement components (C3, C4, and factor B) in human saliva was performed by SDS-PAGE and immunoblotting. Complement C3 was detected as a molecule composed of a 115-kDa alpha-chain linked to a 70-kDa beta chain by disulfide bonds, and C3 levels ranged from 0.52 to 15.0 micrograms/ml (n = 15). ...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1023/a:1027320425291
更新日期：1997-09-01 00:00:00
abstract：:An absolute requirement for monocytes was demonstrated in the T-cell proliferative response to tetanus toxoid (TT) antigen. Antigen-pulsed monocytes were shown to be effective in triggering T-cell proliferation. Using 125I-radiolabeled TT antigen, uptake by monocytes increased progressively over an 18-hr period, at wh...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/BF00915473
更新日期：1981-01-01 00:00:00
abstract：:Increased levels of serum IgD can be found in single patients with a variety of clinical syndromes and in the disease entity designated hyper-IgD syndrome which is associated with periodic fever and lymphadenopathy. We investigated 17 patients, both children and adults, with high serum IgD levels ranging from 220 to 5...
journal_title：Journal of clinical immunology
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1007/BF00917104
更新日期：1989-09-01 00:00:00
abstract：:The complement system plays a central role in defense to encapsulated bacteria through opsonization and membrane attack complex (MAC) dependent lysis. The three activation pathways (classical, lectin, and alternative) converge in the cleavage of C5, which initiates MAC formation and target lysis. C5 deficiency is asso...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/s10875-016-0275-4
更新日期：2016-05-01 00:00:00
abstract：BACKGROUND:Wiskott-Aldrich syndrome (WAS) is a rare primary immunodeficiency disease, with an incidence of 4/1,000,000 live male births. In China, an estimated number of 35 babies with WAS are born each year, but likely many remain undiagnosed. OBJECTIVES:The objectives of study were to review the clinical and molecul...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/s10875-009-9285-9
更新日期：2009-07-01 00:00:00
abstract：INTRODUCTION:Autoantibodies to granulocyte-macrophage colony-stimulating factor (GM-CSF) can cause acquired pulmonary alveolar proteinosis (PAP). Cases of acquired PAP susceptible to typical respiratory pathogens and opportunistic infections have been reported. Anti-GM-CSF autoantibodies have been reported in a few pat...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/s10875-016-0364-4
更新日期：2017-02-01 00:00:00
abstract：:A patient affected with multiple myeloma displayed in the serum, urine, and cerebrospinal fluid a paraprotein with identical electrophoretic mobility. The paraprotein, which was polymeric, appeared in the serum and cerebrospinal fluid mainly as the dimer and tetramer, whereas in the urine the tetramer was predominant....
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/BF00917333
更新日期：1986-07-01 00:00:00
abstract：:We studied immunological functions of peripheral blood lymphocytes (PBL) from human T-cell leukemia virus type I (HTLV-I)-seropositive healthy carriers in vitro. Proliferative responses of PBL to T-cell and B-cell mitogens such as concanavalin A (Con A), pokeweed mitogen (PWM), and Staphylococcus aureus Cowan I (SAC) ...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/BF00918017
更新日期：1989-11-01 00:00:00
abstract：:Among a population of uniformly differentiated TH(2) cells, only a portion express IL-4 upon stimulation and those that do often express the product of only a single allele. We review the evidence for the basis of IL-4 monoallelism and argue that it depends upon probabilistic expression of the Il4 gene. Further, we ar...
journal_title：Journal of clinical immunology
pub_type： 杂志文章,评审
doi：10.1007/s10875-005-8218-5
更新日期：2005-11-01 00:00:00
abstract：:Deficient DNA mismatch repair results in microsatellite instability and might induce shifts of translational reading frames of genes encompassing coding microsatellites. These may be translated in truncated proteins, including neo-peptide tails functioning as tumor rejection antigens, when presented in the context of ...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1023/a:1025329819121
更新日期：2003-09-01 00:00:00
abstract：INTRODUCTION:Experimental allergic encephalomyelitis (EAE) is a T cell-mediated autoimmune disease model for multiple sclerosis (MS). We have shown earlier that 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) and curcumin ameliorate EAE by modulating inflammatory signaling pathways in T lymphocytes. Toll-like rec...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/s10875-008-9202-7
更新日期：2008-09-01 00:00:00
abstract：BACKGROUND:The peroxisome proliferator-activated receptors (PPARs) have been implicated in immune regulation. We determined the transcriptional expression of the three isoforms, PPARalpha, PPARgamma1, and PPARgamma2 in the peritoneal macrophages, CD4- and CD8-positive lymphocytes in non-obese diabetic (NOD) mice at 5 a...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/s10875-009-9300-1
更新日期：2009-09-01 00:00:00
abstract：:Immunoglobulin isolated from sera of homosexual men infected with human immunodeficiency virus (HIV) inhibited the reverse transcriptase (RT) activity of HIV. The inhibitory activity was specifically directed against HIV RT, and not against other mammalian retrovirus RT, including human T-lymphotropic virus type I. Th...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/BF00915727
更新日期：1987-05-01 00:00:00
abstract：:The concentrations of seven immunoglobulin isotypes (IgA, IgE, IgM, IgG1, IgG2, IgG3, and IgG4) were measured in the sera of 207 Finnish blood donors, and they were allotyped with anti-Gm antibodies: anti-f, anti-a, anti-x, and anti-n. The above population could be divided into 12 phenotypes, and significant differenc...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/BF00918793
更新日期：1991-01-01 00:00:00
abstract：BACKGROUND:An outbreak of multisystem inflammatory syndrome in children, including Kawasaki disease (KD), emerged during COVID-19 pandemic. We explored whether Kawasaki-like disease (KD), when associated with confirmed SARS-CoV-2 infection, has specific characteristics. METHODS:We included children and adolescents wit...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/s10875-020-00941-0
更新日期：2021-01-04 00:00:00
abstract：:Immune complexes and 1-O-alkyl-2-acetyl-SN-glycero-3-phosphorylcholine (AGEPC) are potent platelet-activating factors which interact with distinct receptors on human platelets. The mechanisms of platelet activation by these two stimuli were investigated by examining the effects of AGEPC on human platelets which had be...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/BF00915365
更新日期：1986-01-01 00:00:00
abstract：:Using our gp120/41-expressing, NK cell activity-resistant CEM.NKR cell clones as targets in HIV-1-specific antibody-dependent cellular cytotoxicity (ADCC) assays, we demonstrate here that the serum titers of anti-HIV-1 ADCC antibodies bear a significant (P < 0.05) positive correlation with the peripheral blood CD4+ T ...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1023/a:1011087132180
更新日期：2001-05-01 00:00:00
abstract：:Inhaled and intravenously administered adenosine induces mast cell-mediated (histamine-dependent) bronchospasm in asthmatics without causing urticaria. A differential response to adenosine by human lung and skin mast cells is shown: low concentrations potentiate FcεRI-induced degranulation of human lung mast cells but...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/s10875-011-9517-7
更新日期：2011-06-01 00:00:00
abstract：:We read with interest the recent paper in the JoCI, entitled 'Study of the expression of Toll-Like Receptors in Different Histological Types of Colorectal Polyps and Their Relationship with Colorectal Cancer' by Eiró et al. TLR7 and 9 expression is altered in CRC vs. normal control; which is proposed to be correlated ...
journal_title：Journal of clinical immunology
pub_type： 评论,信件
doi：10.1007/s10875-012-9719-7
更新日期：2012-12-01 00:00:00
abstract：:Cross-sectional and limited sequential studies of neutrophil and monocyte chemotaxis were done using cells isolated from cord blood and peripheral blood of 2- to 6-day-old neonates and infants at 4 and 6 months of age. These studies show that cord blood phagocyte chemotaxis is comparable to adult values, whereas phago...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/BF00915228
更新日期：1982-07-01 00:00:00
abstract：BACKGROUND:Primary immunodeficiency disorders (PID) are a group of heterogeneous disorders mainly characterized by severe and recurrent infections and increased susceptibility to malignancies, lymphoproliferative and autoimmune conditions. National registries of PID disorders provide epidemiological data and increase t...
journal_title：Journal of clinical immunology
pub_type： 杂志文章,多中心研究
doi：10.1007/s10875-014-0001-z
更新日期：2014-05-01 00:00:00
abstract：:The role of lymphocytes in the pathogenesis of bullous pemphigoid was examined by assaying the blister fluid obtained from bullous pemphigoid patients for the presence of the lymphokine, lymphotoxin. Blister fluids from six bullous pemphigoid were assayed on L-929 target cells for the presence of cytolytic molecules i...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/BF00915284
更新日期：1984-01-01 00:00:00
abstract：PURPOSE:Tumor necrosis factor alpha-induced protein 3 (TNFAIP3, A20) is a negative regulator of the nuclear factor-κB (NF-κB) pathway. It has recently been recognized that TNFAIP3 deficiency leads to early onset of autoinflammatory and autoimmune syndrome resembling Behçet's disease. Here, we report a novel mutation in...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/s10875-019-00604-9
更新日期：2019-02-01 00:00:00
abstract：PURPOSE:Although many studies have investigated Mendelian susceptibility to mycobacterial disease (MSMD) worldwide, there is no report of the long-term clinical management and prognosis for MSMD in China. METHODS:This is a cohort study from January 2000 to June 2018. Three hundred and twenty-four patients with bacillu...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/s10875-019-00672-x
更新日期：2019-08-01 00:00:00
abstract：INTRODUCTION:Systemic lupus erythematosus (SLE) is a systemic autoimmune disease associated with aberrant activation of T and B lymphocytes. Abnormal activation of intracellular signaling molecules in lymphocytes by inflammatory cytokines can instigate the inflammation in SLE. MATERIALS AND METHODS:The activation of e...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/s10875-009-9318-4
更新日期：2009-11-01 00:00:00
abstract：:Hematopoietic stem cell transplantation (HSCT) is curative for severe combined immunodeficiency (SCID), but data on long-term impact of pre-HSCT chemotherapy, immune reconstitution and quality of life (QoL) of specific SCID genotypes are limited. We evaluated the long-term immune-reconstitution, health outcome and QoL...
journal_title：Journal of clinical immunology
pub_type： 杂志文章
doi：10.1007/s10875-018-0540-9
更新日期：2018-08-01 00:00:00
