Abstract：
PURPOSE:The purposes of our study were to elucidate the role of BRG1 in the development of human glioma and to determine the effect of BRG1 on glioma cell growth, migration and invasion. METHODS:Using tissue microarray and immunohistochemistry, we evaluated BRG1 staining in 190 glioma tissues, 8 normal brain tissues and 8 tumor adjacent normal brain tissues. We studied glioma cell proliferative ability with reduced BRG1 expression by siRNA using CCK-8 cell proliferation assay and cell cycle analysis. We studied the role of BRG1 in glioma cell migration and invasion by cell migration assay and matrigel invasion assay. We performed western blot to detect cyclin D1, cyclin B1 and MMP-2 protein expression. We also detected MMP-2 enzyme activity by gelatin zymography. RESULTS:Our results showed that BRG1 expression was increased in benign tumor and malignant tumor compared with tumor adjacent normal brain tissue (P < 0.01 for both). We did not find any correlation between BRG1 expression and clinicopathological parameters. In addition, we found that knockdown of BRG1 in glioma cell lines inhibits cell growth due to the G1 phase arrest by downregulating cyclin D1. We further demonstrated that silencing of BRG1 in glioma cells inhibited the cell migration and invasion abilities, and downregulation of MMP-2 expression greatly contributed to the reduced cell invasion and migration abilities. CONCLUSIONS:Our data indicated that BRG1 expression is significantly increased in human glioma and it may be involved in the process of glioma cell proliferation, migration and invasion.
journal_name
J Cancer Res Clin Oncoljournal_title
Journal of cancer research and clinical oncologyauthors
Bai J,Mei PJ,Liu H,Li C,Li W,Wu YP,Yu ZQ,Zheng JNdoi
10.1007/s00432-012-1172-8subject
Has Abstractpub_date
2012-06-01 00:00:00pages
991-8issue
6eissn
0171-5216issn
1432-1335journal_volume
138pub_type
杂志文章abstract：OBJECTIVE:To evaluate through retrospective analysis the efficacy and toxicity of combination chemotherapy with etoposide, vincristine, doxorubin and dexamethasone (EVAD) as second-line therapy in patients with advanced AIDS-related Kaposi's sarcoma (AIDS-KS) after failure of first-line chemotherapy. METHODS:Eighty-ei...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-011-1109-7
更新日期：2012-03-01 00:00:00
abstract：PURPOSE:As previously described, SPC/myc transgenic mice developed bronchioloalveolar adenocarcinomas derived from alveolar type II (AT II) cells within 10-14 months, whereas SPC/IgEGF transgenic mice developed hyperplasias. Our purpose was to determine the potential interplay of environmental and genetic factors in lu...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-002-0400-z
更新日期：2003-02-01 00:00:00
abstract：PURPOSE:Promising new therapies for neoplasia include tyrosine kinase receptor antagonists. Tyrosine kinase oncogenes present an appealing anti-tumor drug target since they play an integral role in a variety of cellular responses including cell proliferation and differentiation. We previously demonstrated a high rate o...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-004-0545-z
更新日期：2004-04-01 00:00:00
abstract：:A prospective, randomized, double-blind placebo-controlled trial was designed to evaluate the impact of granulocyte/macrophage-colony-stimulating factor (GM-CSF) on the efficacy of sequential high-dose cytosine arabinoside/mitoxantrone chemotherapy (S-HAM) in adult patients with high-risk myelodysplastic syndromes (MD...
journal_title：Journal of cancer research and clinical oncology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/s004320050288
更新日期：1999-01-01 00:00:00
abstract：PURPOSE:The introduction of immunochemotherapy has led to a significant improvement in treatment results and prognosis of diffuse large B-cell non-Hodgkins lymphoma (DLBCL) both at initial diagnosis and in relapse. Trofosfamide, an oxazaphosphorine derivative, has been utilized as alternative treatment option for patie...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-018-2772-8
更新日期：2019-01-01 00:00:00
abstract：PURPOSE:In the present study an antigen-mimetic peptide of the anti-JL1 leukemia-specific monoclonal antibody (mAb) was identified and characterized. METHODS:From combinatorial peptide phage display libraries displaying the random linear heptapeptides and dodecapeptides, we selected clones with affinity to anti-JL1 mA...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-002-0390-x
更新日期：2002-12-01 00:00:00
abstract：PURPOSE:To evaluate the pattern of recurrence of breast cancer according to its biological subtype in a large cohort of patients treated with therapy representative of current practice. PATIENTS AND METHODS:Patients treated between 2000 and 2016 with known biological subtype were eligible. Data were prospectively coll...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-018-2644-2
更新日期：2018-07-01 00:00:00
abstract：PURPOSE:To evaluate the role of radioembolization and systemic chemotherapy as a combined modality therapy for unresectable colorectal liver metastases. PATIENTS AND METHODS:Prospective database of a major yttrium-90 microsphere radioembolization treatment center in Sydney, Australia, that included 140 patients with u...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-010-0948-y
更新日期：2011-05-01 00:00:00
abstract：:Carboplatin and interferon beta (IFN beta) were tested alone and in combination for their antiproliferative activity on the human melanoma cell line SK-MEL 28 in vitro. Cells were incubated for 4 days in the presence of carboplatin (0.1 mM and 0.1 microM) and interferon beta (5 pM and 5 nM) and cell growth inhibition ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01202218
更新日期：1995-01-01 00:00:00
abstract：PURPOSE:Constructed from a theoretical framework, the coordinated undermining of survival paths in glioblastoma (GBM) is a combination of nine drugs approved for non-oncological indications (CUSP9; aprepitant, auranofin, captopril, celecoxib, disulfiram, itraconazole, minocycline, quetiapine, and sertraline) combined w...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-019-02920-4
更新日期：2019-06-01 00:00:00
abstract：:To investigate multidrug-resistance gene (MDR1) promoter efficacy and drug inducibility in cells with different multidrug-resistance phenotypes, multidrug-resistant HCT15 and drug-sensitive KM12 human colon carcinoma cell lines were transfected with constructs incorporating the chloramphenicol acetyltransferase (CAT) ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01261403
更新日期：1996-01-01 00:00:00
abstract：BACKGROUND:Two hundred and fourteen patients with benign giant cell tumor of bone (GCTB), treated from 1980 to 2007 at the Department of Orthopedics of the University of Muenster (Germany), were analyzed in a retrospective study. PATIENTS AND METHODS:The mean age was 33.3 years with a female-to-male ratio of 1.2 : 1. ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-008-0370-x
更新日期：2008-09-01 00:00:00
abstract：:Cancer development depends on the aberrant activation of signal transduction pathways that control cell growth and survival and play important roles in normal embryonic development. This review will focus on one of the most powerful pathways, the canonical Wnt signal transduction cascade, which has been originally des...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章,评审
doi：10.1007/s00432-003-0431-0
更新日期：2003-04-01 00:00:00
abstract：:The laboratory findings in patients receiving high-dose vitamin A as adjuvant treatment for stage I lung cancer are here reported. A group of 283 patients were randomized to either treatment with retinyl palmitate (300,000 IU daily for 12 months) or standard follow-up, and are now evaluable after a median observation ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/BF01613140
更新日期：1991-01-01 00:00:00
abstract：OBJECTIVE:The objective of this study was to summarize the accuracy of preoperative vascular invasion with endoscopic ultrasound (EUS) and computed tomography (CT) test performance in pancreatic cancer with meta-analysis METHOD:Two reviewers searched MEDLINE database to identify relevant studies. The reference lists o...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s00432-014-1728-x
更新日期：2014-12-01 00:00:00
abstract：PURPOSE:Stathmin (Oncoprotein18), a ubiquitous and highly conserved 19-kDa cytosolic phosphoprotein, has been reported to play a critical role in mitosis and possibly other cellular processes, which is associated with tumor carcinogenesis and development. The purpose of this study was to examine the involvement of stat...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-008-0520-1
更新日期：2009-06-01 00:00:00
abstract：:Insulin-like growth factor I (IGF-I) exerts pleiotropic effects on mammalian cells via stimulation of its receptor (IGF-IR), a receptor tyrosine kinase. In vivo, IGF-I acts both as a local tissue growth factor and as a circulating hormone. In oncological research, IGF-I has received increased attention as the activate...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章,评审
doi：10.1007/s004320050259
更新日期：1999-01-01 00:00:00
abstract：PURPOSE:Long interspersed nuclear element 1 (LINE-1 or L1) is a dominant non-long terminal repeat (non-LTR) retrotransposon in the human genome that has been implicated in the overexpression of MET. Both the canonical MET and L1-MET transcripts are considered to play a role in hepatocellular carcinoma (HCC) development...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-020-03395-4
更新日期：2021-01-01 00:00:00
abstract：:The antitumor and anti-metastatic effects of a potent angiogenesis inhibitor, O-(chloroacetyl-carbamoyl)fumagillol (TNP-470), was investigated in a highly metastatic model of human hepatocellular carcinoma-LCI-D20. Small pieces of LCI-D20 tumor tissue were implanted subcutaneously into the right axillary region of 24 ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01240121
更新日期：1997-01-01 00:00:00
abstract：:Bromelain, a pineapple-derived plant product, added to C57Bl/6 mice laboratory chow decreased lung metastasis of Lewis lung cancer cells implanted s.c. This antimetastatic potential was demonstrated by both the active and inactive bromelain with or without proteolytic, anticoagulant properties. ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00391501
更新日期：1988-01-01 00:00:00
abstract：PURPOSE:Results from in vitro chemosensitivity testing recommend treosulfan/gemcitabine chemotherapy for the treatment of stage IV uveal melanoma. METHODS:Twenty patients received treosulfan 3,500 mg/m2 followed by gemcitabine 1,000 mg/m2 on day 1 and day 8 repeated on day 29. In cases of prior chemotherapy only 75% o...
journal_title：Journal of cancer research and clinical oncology
pub_type： 临床试验,杂志文章
doi：10.1007/s00432-004-0569-4
更新日期：2004-07-01 00:00:00
abstract：PURPOSE:The precise determination of energy metabolites is challenged by the heterogeneity of their distribution, their rapid changes after surgical resection and the architectural complexity of malignancies. Induced metabolic bioluminescence imaging (imBI) allows to determine energy metabolites in tissue sections and ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-016-2200-x
更新日期：2016-09-01 00:00:00
abstract：:Stereological point-counting methods were used to determine the volumetric alterations in collagen from the lamina propria immediately beneath the epithelial-connective tissue junction in hamster check-pouch mucosa treated with the chemical carcinogen DMBA. In addition, a non-neoplastic inflammatory control was evalua...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01032605
更新日期：1984-01-01 00:00:00
abstract：PURPOSE:Cancer incidence in the elderly population has been continuously rising, and their treatment is an increasing concern among oncologists. This study aimed to evaluate the incidence and risk factors for morbidity and mortality after major oncological surgery in elderly patients with head and neck squamous cell ca...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-016-2141-4
更新日期：2016-06-01 00:00:00
abstract：PURPOSE:The t(11;18)(q21;q21) chromosome translocation is frequent in gastric MALT lymphoma, but the t(11;18)(q21;q21) chromosome translocation is very rare in other sites of MALT lymphomas. We investigated the possibility that MALT lymphoma occurred in the right buccal mucosa of a 66-year-old Japanese woman who had th...
journal_title：Journal of cancer research and clinical oncology
pub_type： 信件
doi：10.1007/s00432-010-0939-z
更新日期：2010-11-01 00:00:00
abstract：PURPOSE:The incidence and mortality of gastric and colorectal cancers are among the highest malignant tumors in China. The aim of this study is to investigate whether variations of the human oxoguanine glycosylase 1 (hOGG1) gene are related to the risk of gastric and colorectal cancers in the Chinese population. METHO...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-011-1022-0
更新日期：2011-10-01 00:00:00
abstract：PURPOSE:The N-myc downstream regulated gene 1 (NDRG1)/Cap43 is overexpressed in multiple cancer types, including lung cancer. In this study, we investigated the expression of NDRG1/Cap43 in lung cancer tissues and in serum from 90 lung cancer patients and 60 healthy controls. MATERIALS AND METHODS:Ninety patients with...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-012-1236-9
更新日期：2012-11-01 00:00:00
abstract：:The arabinofuranosylcytosine (AraC) derivative N4-octadecyl-1-beta-D-arabinofuranosylcytosine (NOAC) and its (5'-->5')-heterodinucleoside phosphate analog NOAC-AraC were compared with AraC for cytotoxicity, cell-cycle dependence, phosphorylation by deoxycytidine (dC) kinase and apoptosis induction in native, AraC- or ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s004320050349
更新日期：2000-06-01 00:00:00
abstract：:As currently recognized, the essential aspects of ovarian carcinoma surgery are: (1) the search for occult metastases in tumors located in the lower pelvis, (2) the maximum tumor removal ("tumor-reductive surgery") in advanced ovarian carcinoma and (3) the exploratory laparotomy to confirm a complete clinical remissio...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00399377
更新日期：1984-01-01 00:00:00
abstract：PURPOSE:MicroRNAs are small, non-coding RNAs that are critical regulators of various diseases including cancer, and may represent a novel class of cancer biomarkers. Recent reports have highlighted the oncogenic aspects of miR-125b. However, the level and clinical relevance of circulating miR-125b transcripts in human ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-012-1285-0
更新日期：2012-12-01 00:00:00
