当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Cycling hypoxia up-regulates thioredoxin levels in human MDA-MB-231 breast cancer cells.

Cycling hypoxia up-regulates thioredoxin levels in human MDA-MB-231 breast cancer cells.

Abstract：

:The thioredoxin system is a key cellular antioxidant system and is highly expressed in cancer cells, especially in more aggressive and therapeutic resistant tumors. We analysed the expression of the thioredoxin system in the MDA-MB-231 breast cancer cell line under conditions mimicking the tumor oxygen microenvironment. We grew breast cancer cells in either prolonged hypoxia or hypoxia followed by various lengths of reoxygenation and in each case cells were cultured with or without a hypoxic cycling preconditioning (PC) phase preceding the hypoxic growth. Flow cytometry-based assays were used to measure reactive oxygen species (ROS) levels. Cells grown in hypoxia showed a significant decrease in ROS levels compared to normoxic cells, while a significant increase in ROS levels over normoxic cells was observed after 4 h of reoxygenation. The PC pre-treatment did not have a significant effect on ROS levels. Thioredoxin levels were also highest after 4 h of reoxygenation, however cells subjected to PC pre-treatment displayed even higher thioredoxin levels. The high level of intracellular thioredoxin was also reflected on the cell surface. Reporter assays showed that activity of the thioredoxin and thioredoxin reductase gene promoters was also highest in the reoxygenation phase, although PC pre-treatment did not result in a significant increase over non-PC treated cells. The use of a dominant negative Nrf-2 negated the increased thioredoxin promoter activity during reoxygenation. This data suggests that the high levels of thioredoxin observed in tumors may arise due to cycling between hypoxia and reoxygenation.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Karlenius TC,Shah F,Di Trapani G,Clarke FM,Tonissen KF

doi

10.1016/j.bbrc.2012.02.027

subject

Has Abstract

pub_date

2012-03-09 00:00:00

pages

350-5

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(12)00245-8

journal_volume

419

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development.

    abstract：:Cancer cell molecular mimicry of stem cells (SC) follows with enhanced proliferative and renewal capacities. In support, numerous mediators of SC self-renewal have been evinced to exhibit oncogenic potential. More and more researches showed that the embryonic stem cell self-renewal genes express in various cancer cell...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.06.016

    authors： Deng L,Xiang X,Yang F,Xiao D,Liu K,Chen Z,Zhang R,Feng G

    更新日期：2017-08-19 00:00:00

  • Involvement of superoxide radical in signal transduction regulating stomatal movements.

    abstract：:Stomatal movements are regulated by alternate phase transition in the lipophilic domains of membrane initiated by blue light through activation of plasmalemma redox system. ABA blocks the blue light activated redox reactions leading to the accumulation of superoxide (O2.-) radical inducing melting of the membrane and ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2625

    authors： Purohit S,Kumar GP,Laloraya M,Laloraya MM

    更新日期：1994-11-30 00:00:00

  • Pyrophosphate functions as phosphoryl donor with UDP-glucose-treated mammalian phosphofructokinase.

    abstract：:Phosphofructokinase of rabbit muscle, which is specific for nucleoside triphosphates such as ATP, dissociated and gained the capability to utilize pyrophosphate as phosphoryl donor following incubation with UDP-glucose. The pyrophosphate- and ATP-linked activities of UDP-glucose-treated muscle phosphofructokinase were...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)90754-x

    authors： Wong JH,Balogh A,Buchanan BB

    更新日期：1984-06-29 00:00:00

  • Insoluble matrix components of glioma cells suppress LPS-mediated iNOS/NO induction in microglia.

    abstract：:This study examines the influence of insoluble matrix components of glioma (astrocytoma) cells on LPS-mediated inducible nitric oxide (NO)/NO synthase (iNOS) induction in microglia cells. Insoluble matrix components prepared from C6 rat glioma cells strongly suppressed iNOS induction and subsequent NO release induced ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.06.149

    authors： Kim YJ,Hwang SY,Han IO

    更新日期：2006-09-01 00:00:00

  • Induction of histidine decarboxylase in rat basophilic leukemia cells by interferon and prevention of its effect in coincubation with ADP-ribosyltransferase inhibitors.

    abstract：:Treatment of rat basophilic leukemia cell line (2H3) with interferon-alpha significantly increased intracellular histamine levels. On the other hand, the histidine content was decreased reciprocally by interferon in a dose-dependent manner. Concomitantly, the activity of histidine decarboxylase, the enzyme responsible...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(89)80181-0

    authors： Kito T,Kubota M,Takimoto T,Akiyama Y,Heike T,Mikawa H

    更新日期：1989-01-16 00:00:00

  • Influence of cholesterol and ergosterol on membrane dynamics: a fluorescence approach.

    abstract：:Sterols are essential membrane components of eukaryotic cells and are important for membrane organization and function. Cholesterol is the most representative sterol present in higher eukaryotes. It is often found distributed non-randomly in domains or pools in biological and model membranes. Cholesterol-rich function...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.04.118

    authors： Arora A,Raghuraman H,Chattopadhyay A

    更新日期：2004-06-11 00:00:00

  • The eta isoform of protein kinase C inhibits UV-induced activation of caspase-3 in normal human keratinocytes.

    abstract：:Protein kinase C (PKC) fulfills a central role in the decision of cell fate in keratinocytes. Both PKC delta and PKC eta induce growth inhibition and differentiation of normal human keratinocytes (NHK). Here we show that PKC delta and PKC eta play opposite roles in UVB-induced apoptosis in NHK. PKC delta enhanced UVB-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00345-0

    authors： Matsumura M,Tanaka N,Kuroki T,Ichihashi M,Ohba M

    更新日期：2003-03-28 00:00:00

  • Compounds stimulating cytosolic phospholipase A2 activity with a combinational action mode.

    abstract：:The screening of small synthetic compound libraries is a useful means of identifying molecules that modulate various cellular responses. We screened more than 10,000 different small compounds and identified three synthetic compounds that stimulate arachidonic acid (AA) release in a combinational manner in neutrophil-l...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.10.063

    authors： Bae YS,Park EY,Lee HY,Kang HK,Suh PG,Kwak JY,Ryu SH,Lee T

    更新日期：2004-12-10 00:00:00

  • Hes1-deficient mice show precocious differentiation of Paneth cells in the small intestine.

    abstract：:We have previously shown that Hes1 is expressed both in putative epithelial stem cells just above Paneth cells and in the crypt base columnar cells between Paneth cells, while Hes1 is completely absent in Paneth cells. This study was undertaken to clarify the role of Hes1 in Paneth cell differentiation, using Hes1-kno...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.12.174

    authors： Suzuki K,Fukui H,Kayahara T,Sawada M,Seno H,Hiai H,Kageyama R,Okano H,Chiba T

    更新日期：2005-03-04 00:00:00

  • A novel role for earthworm peptide Lumbricusin as a regulator of neuroinflammation.

    abstract：:Recently, we reported that Lumbricusin, an antimicrobial peptide isolated from earthworm Lumbricus terrestris, enhanced neuronal proliferation and ameliorated motor dysfunction and dopaminergic neurodegeneration. Accumulating evidence suggests that neurodegeneration is the primary pathological feature of acute or chro...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.06.154

    authors： Seo M,Lee JH,Baek M,Kim MA,Ahn MY,Kim SH,Yun EY,Hwang JS

    更新日期：2017-08-26 00:00:00

  • An RNA oligonucleotide corresponding to the polypyrimidine region of the rat alpha 1(I) procollagen promoter forms a stable triplex and inhibits transcription.

    abstract：:In this report we demonstrate formation of a triplex structure by an antiparallel RNA oligonucleotide corresponding to the 21 bp polypurine-pyrimidine stretch from -141 to -162 of the rat alpha 1(I) procollagen promoter with a Kd of 0.1-0.2 microM. The formation of triplexes by the triplex forming oligoribonucleotide ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9113

    authors： Ririe SS,Guntaka RV

    更新日期：1998-08-10 00:00:00

  • Intracellular location of polyamines associated to red blood cells.

    abstract：:The polyamines associated to human erythrocytes from healthy donors are mainly localized intracellularly. In fact chromatography of the erythrocytes on a resin which has a high affinity and capacity for polyamines does not affect the amount of polyamines associated to the erythrocytes. The low ability of spermine to a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90400-6

    authors： Tadolini B,Hakim G,Orlandini G,Casti A

    更新日期：1986-02-13 00:00:00

  • Cathepsin Q, a novel lysosomal cysteine protease highly expressed in placenta.

    abstract：:The complete nucleotide sequence of a novel cathepsin cDNA derived from rat placenta was determined and is termed cathepsin Q. The predicted protein of 343 amino acid is a member of the family C1A protease related to cathepsin L. Rat cathepsin Q and its mouse counterpart were found highly expressed in placenta, wherea...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.2051

    authors： Sol-Church K,Frenck J,Mason RW

    更新日期：2000-01-27 00:00:00

  • Adaptive increase in pyruvate dehydrogenase kinase 4 during starvation is mediated by peroxisome proliferator-activated receptor alpha.

    abstract：:Pyruvate dehydrogenase kinase isoform 4 (PDK4) is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5608

    authors： Wu P,Peters JM,Harris RA

    更新日期：2001-09-21 00:00:00

  • Endothelin-1 is produced and secreted by neonatal rat cardiac myocytes in vitro.

    abstract：:Production of endothelin-1 in the cultured neonatal rat cardiac myocytes was investigated. Studies on the cultured cardiac myocytes with reverse transcription-polymerase chain reaction followed by Southern hybridization indicated that mRNA of ET-1(preproET-1 mRNA) was expressed by cardiac myocytes. ET-1 polypeptide wa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1291

    authors： Suzuki T,Kumazaki T,Mitsui Y

    更新日期：1993-03-31 00:00:00

  • RecA-ssDNA filaments supercoil in the presence of single-stranded DNA-binding protein.

    abstract：:Using atomic force microscopy (AFM), we find that RecA-single-stranded DNA (RecA-ssDNA) filaments, in the presence of single-stranded DNA-binding (SSB) protein, organize into left-handed bundles, which differ from the previously reported disordered aggregates formed when SSB is excluded from the reaction. In addition,...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.04.014

    authors： Shi WX,Larson RG

    更新日期：2007-06-08 00:00:00

  • Regulatory effect of the glial Golli-BG21 protein on the full-length murine small C-terminal domain phosphatase (SCP1, or Golli-interacting protein).

    abstract：:The gene in the oligodendrocyte lineage (golli) encodes a number of proteins essential for myelination, comprising Golli and classic isoforms that are expressed in a developmentally-regulated manner. The Golli-interacting-protein (GIP) was previously discovered in a search for potential interacting partners of the Gol...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.04.050

    authors： Jaramillo-Tatis S,Vassall KA,Bamm VV,Harauz G

    更新日期：2014-05-16 00:00:00

  • Phosphorylation of protamines by protein kinase C: involvement of sites which are phosphorylated in vivo and are not affected by cAMP-dependent protein kinase.

    abstract：:Most fish protamines contain two phosphorylatable sites both of which incorporate phosphate in vivo. Here we show that in two protamines (salmine A1 and clupeine Y1) the site more distant from the N-terminus (residues 20-21) is unaffected by cAMP-dependent protein kinase while it represents the main target for protein...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)91455-0

    authors： Ferrari S,Pinna LA

    更新日期：1987-05-14 00:00:00

  • Antiproliferative effect of 1,25-dihydroxyvitamin D3 and its analogs on human colon adenocarcinoma cells (CaCo-2): influence of extracellular calcium.

    abstract：:Depending on culture in either "low Ca++" (0.25 mM) or "normal Ca++" (1.8 mM) medium, human colon adenocarcinoma-derived CaCo-2 cells exhibit differential sensitivity to the antiproliferative action of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) and of two side-chain modified analogs, 1,25S,26-trihydroxy-delta 22-vitamin D...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91333-8

    authors： Cross HS,Huber C,Peterlik M

    更新日期：1991-08-30 00:00:00

  • The effect of gastric inhibitory polypeptide on intestinal glucose absorption and intestinal motility in mice.

    abstract：:Gastric inhibitory polypeptide (GIP) is released from the small intestine upon meal ingestion and increases insulin secretion from pancreatic β cells. Although the GIP receptor is known to be expressed in small intestine, the effects of GIP in small intestine are not fully understood. This study was designed to clarif...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.11.077

    authors： Ogawa E,Hosokawa M,Harada N,Yamane S,Hamasaki A,Toyoda K,Fujimoto S,Fujita Y,Fukuda K,Tsukiyama K,Yamada Y,Seino Y,Inagaki N

    更新日期：2011-01-07 00:00:00

  • RANKL blockade suppresses pathological angiogenesis and vascular leakage in ischemic retinopathy.

    abstract：:Receptor activator of NF-κB ligand (RANKL) is a member of the TNF superfamily. RANKL increases endothelial permeability and induces angiogenesis, suggesting its critical roles in the vasculature. Despite the evidence implicating RANKL in vascular pathology, its role in ischemic retinopathy has not been previously repo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.06.046

    authors： Ock S,Park S,Lee J,Kim J

    更新日期：2019-08-20 00:00:00

  • HMG CoA reductase and LDL receptor genes are regulated differently by 15-ketosterols in Hep G2 cells.

    abstract：:The ability of two 15-ketosubstituted sterols, 5alpha-cholest-8(14)-en-3beta-ol-15-one and 3beta-(2-hydroxyethoxy)-5alpha-cholest-8(14)-en-15-one, to alter the mRNA levels of 3-hydroxy-3-methylglutaryl-CoA reductase, low density lipoprotein receptor, and oxysterol binding protein was studied and compared with the effe...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0844

    authors： Kisseleva AF,Goryunova LE,Planells R,Lafont H,Alquier C

    更新日期：1999-06-16 00:00:00

  • DNA damage, lysosomal degradation and Bcl-xL deamidation in doxycycline- and minocycline-induced cell death in the K562 leukemic cell line.

    abstract：:We investigated mechanisms of cytotoxicity induced by doxycycline (doxy) and minocycline (mino) in the chronic myeloid leukemia K562 cell line. Doxy and mino induced cell death in exposure-dependent manner. While annexin V/propidium iodide staining was consistent with apoptosis, the morphological changes in Giemsa sta...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.05.043

    authors： Fares M,Abedi-Valugerdi M,Hassan M,Potácová Z

    更新日期：2015-07-31 00:00:00

  • Gene structure of rat cholecystokinin type-A receptor.

    abstract：:Little is known about the gene structure and mRNA expression of cholecystokinin type-A(CCKA) receptor in animals or in human. By screening an EMBL phage library containing partially digested Long-Evans rat DNA, we isolated 7 genomic clones covering a continuous 27 kb region over which a rat CCKA receptor gene spanned....

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2222

    authors： Takata Y,Takiguchi S,Funakoshi A,Kono A

    更新日期：1995-08-24 00:00:00

  • Fluorescence investigations of calmodulin hydrophobic sites.

    abstract：:Calmodulin activation of target enzymes depends on the interaction between calmodulin hydrophobic regions and some enzyme areas. The Ca2+ induced exposure of calmodulin hydrophobic sites was studied by means of 2-p-toluidinylnaphthalene-6-sulfonate, a fluorescent probe. Scatchard and Job plots showed that the calmodul...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)90896-9

    authors： Follenius A,Gerard D

    更新日期：1984-03-30 00:00:00

  • Synthesis and characterization of folate-PEG-grafted-hyperbranched-PEI for tumor-targeted gene delivery.

    abstract：:A great challenge for gene therapy is to develop a high efficient gene delivery system with low toxicity. Nonviral vectors are still attractive although the current agents displayed some disadvantages (i.e., low transfection efficiency, high toxicity). To overcome the high toxicity of poly(ethylene imine) (PEI) and lo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.01.024

    authors： Liang B,He ML,Xiao ZP,Li Y,Chan CY,Kung HF,Shuai XT,Peng Y

    更新日期：2008-03-21 00:00:00

  • Interaction of activated Rab5 with actin-bundling proteins, L- and T-plastin and its relevance to endocytic functions in mammalian cells.

    abstract：:Rab5 is a GTP-binding protein that is crucial for endocytic machinery functions. We previously identified L-plastin as a binding protein for Rab5, using an affinity column with constitutively active Rab5. L- and T-plastin are isoforms of a plastin protein family belonging to actin-bundling proteins that are implicated...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.03.082

    authors： Hagiwara M,Shinomiya H,Kashihara M,Kobayashi K,Tadokoro T,Yamamoto Y

    更新日期：2011-04-15 00:00:00

  • Inhibition of endothelin-1 induced myocardial protein synthesis by an antisense oligonucleotide against the early growth response gene-1.

    abstract：:We explored the role of the recently discovered "early growth response gene-1 (Egr-1)" in the induction of myocardial protein synthesis by endothelin-1. Endothelin-1 stimulated protein synthesis (i.e. 3H-phenylalanine incorporation) in isolated adult rat cardiomyocytes more than 2-fold. Addition of a 15mer Egr-1 antis...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81376-2

    authors： Neyses L,Nouskas J,Vetter H

    更新日期：1991-11-27 00:00:00

  • Analysis of gene expression in single human oocytes and preimplantation embryos.

    abstract：:Little is known about the gene expression in human oocytes and early embryo development because of the rare availability of the materials. The recent advancement of biotechnology has allowed one to analyze the gene expression in single human oocytes and preimplantation embryos. Gene expression of human lactate dehydro...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.11.149

    authors： Li SS,Liu YH,Tseng CN,Singh S

    更新日期：2006-02-03 00:00:00

  • Alternative pathway linked by hydrogen bonds connects heme-Fe of cytochrome c with subunit II-CuA of cytochrome a.

    abstract：:The bridging element for electron transfer in proteins is the hydrogen bond according to the new experimental perspective in preference to carbon-carbon σ-bond presently used. The purpose of this study is to identify an alternative pathway linked by hydrogen bonds suitable for electron transfer from heme-Fe of cytochr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.09.141

    authors： Ramasarma T,Vaigundan D

    更新日期：2018-10-28 00:00:00