Abstract:
:Mantle cell lymphoma (MCL) and small lymphocytic lymphoma (SLL) exhibit similar but distinct immunophenotypic profiles. Many cases can be diagnosed readily by flow cytometry (FCM) alone; however, ambiguous cases are frequently encountered and necessitate additional studies, including immunohistochemical staining for cyclin D1 and fluorescence in situ hybridization for IgH-CCND1 rearrangement. To determine if greater diagnostic accuracy could be achieved from FCM data alone, we developed an unbiased, machine-based algorithm to identify features that best distinguish between the 2 diseases. By applying conventional diagnostic criteria to the flow cytometry data, we were able to assign 28 of 44 (64%) MCL and 48 of 70 (69%) SLL cases correctly. In contrast, we were able to assign all 44 (100%) MCL and 68 of 70 (97%) SLL cases correctly using a novel set of criteria, as identified by our automated approach. The most discriminating feature was the CD20/CD23 mean fluorescence intensity ratio, and we found unexpectedly that inclusion of FMC7 expression in the diagnostic algorithm actually reduced its accuracy. This study demonstrates that computational methods can be used on existing clinical FCM data to improve diagnostic accuracy and suggests similar computational approaches could be used to identify novel prognostic markers and perhaps subdivide existing or define new diagnostic entities.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Zare H,Bashashati A,Kridel R,Aghaeepour N,Haffari G,Connors JM,Gascoyne RD,Gupta A,Brinkman RR,Weng APdoi
10.1309/AJCPMMLQ67YOMGEWsubject
Has Abstractpub_date
2012-01-01 00:00:00pages
75-85issue
1eissn
0002-9173issn
1943-7722pii
137/1/75journal_volume
137pub_type
杂志文章abstract::The authors illustrate two neoplasms arising in the pretibial soft tissue that are histologically identical to adamantinoma of the long bones. The tumors developed in a 24-year-old woman and a 31-year-old man. Both were treated by local excision. Histologically, one tumor had a tubular pattern and one was composed of ...
journal_title:American journal of clinical pathology
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更新日期:1985-01-01 00:00:00
abstract::We describe a method of spectrophotometric detection of BCR/ABL chimeric sequences amplified by multiplex reverse transcriptase-polymerase chain reaction (RT-PCR), enabling the use of archival hematologic slides as RNA sources. Multiplex PCR amplified b3a2, b2a2, and e1a2 break points of the BCR/ABL translocation and ...
journal_title:American journal of clinical pathology
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更新日期:1997-10-01 00:00:00
abstract::Sporadically, hematology analyzers generate grossly erroneous results for one or several parameters of the complete blood count (CBC) because of a characteristic or peculiarity of an individual patient specimen. This article presents three cases of relatively less well-known analytic errors of this sort, falsely eleva...
journal_title:American journal of clinical pathology
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doi:10.1093/ajcp/92.3.295
更新日期:1989-09-01 00:00:00
abstract::Three cases of infantile hemangioendothelioma were immunohistochemically studied with the use of antibodies against von Willebrand factor (vWF), Ulex europaeus I lectin (UEA I), vimentin, thrombomodulin (TM), and actin, as endothelial cell (EC) markers. Because of a broad variety of histologic features, the growth pat...
journal_title:American journal of clinical pathology
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更新日期:1989-06-01 00:00:00
abstract::Previous studies have found that amplification and overexpression of the c-erbB-2 oncogene in mammary ductal adenocarcinomas predicts decreased disease-free or overall survival. Information regarding the prognostic and pathogenetic significance of oncogene amplification has been limited by difficulty obtaining suffici...
journal_title:American journal of clinical pathology
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更新日期:1994-04-01 00:00:00
abstract::Plasma samples from patients with severe classic hemophilia, suspected of having mild inhibitors not detected in the standard Bethesda inhibitor test, were examined in modified Bethesda tests and in a screening test based on the activated partial thromboplastin time (APTT). The APTT test was more sensitive to mild inh...
journal_title:American journal of clinical pathology
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更新日期:1982-06-01 00:00:00
abstract::Overexpression and amplification of the HER-2 oncogene in patients with breast cancer has correlated with early onset of metastasis, resistance to hormonal therapy and some forms of chemotherapy, and shortened survival. Therefore, evaluation of this putative prognostic or predictive factor seems critical. Because diff...
journal_title:American journal of clinical pathology
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更新日期:2000-05-01 00:00:00
abstract::Human intravenous immunoglobulins prepared by the cold ethanol fractionation technique of Cohn are considered safe with respect to infectivity. However, there have been several instances of transmission of both hepatitis B and non-A,non-B hepatitis viruses after administration of intravenous immunoglobulins. To determ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/97.1.108
更新日期:1992-01-01 00:00:00
abstract::Pulmonary alveolar proteinosis (PAP) is an uncommon disease in which alveoli are progressively filled with surfactant-related material. Although a definitive diagnosis is usually made by an open lung biopsy, bronchoalveolar lavage (BAL) cytology may play a decisive role in the clinical work-up of these patients, and, ...
journal_title:American journal of clinical pathology
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更新日期:1996-10-01 00:00:00
abstract::A case of Torulopsis glabrata endocarditis involving the mitral valve is reported. Endocarditis developed during convalescence from severe abdominal trauma and was associated with fungemia. Morphologically characteristic organisms were present in the mitral valve. ...
journal_title:American journal of clinical pathology
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更新日期:1978-09-01 00:00:00
abstract::Breast cancer is the result of a multistage carcinogenic process. Initiation, promotion, dependency and autonomy make up a sequence of experimentally distinguishable phases of this process. Progression--the transition from dependency on hormonal support to autonomy--is demonstrable clinically. High-affinity saturatabl...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1093/ajcp/64.6.797
更新日期:1975-12-01 00:00:00
abstract::p53 protein expression was evaluated in a series of 204 primary colorectal adenocarcinomas by immunohistochemistry using frozen tissue sections and monoclonal antibody DO-7. Nuclear staining of more than 5% of neoplastic cells was observed in 124 (60.8%) adenocarcinomas, which were classified as p53 positive. p53 immu...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/105.5.604
更新日期:1996-05-01 00:00:00
abstract::Although acute myeloid leukemias (AMLs) cytochemically negative for myeloperoxidase are now well recognized, myeloid surface antigen-negative AMLs are rare. The morphologic, cytochemical, immunologic, and cytogenetic or molecular features of such cases are described in four adults aged 19 to 60 years. All had AML with...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/107.1.68
更新日期:1997-01-01 00:00:00
abstract::In 1985 Harvard Medical School initiated an experimental curriculum that incorporated many of the recommendations of the report on the General Professional Education of the Physician (GPEP). Key features are problem-based small group tutorials that emphasize active learning, with increased independent study time and a...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1989-10-01 00:00:00
abstract::This study describes results of fresh-frozen immunohistochemistry applied to two typical Warthin's tumors. Polyclonal follicular center cell staining and an apparent reduction in the number of T-lymphocytes were found. These observations tend to support the concept that the lymphoid infiltrate in Warthin's tumor repre...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/74.6.795
更新日期:1980-12-01 00:00:00
abstract::Diarrheal diseases caused by bacterial pathogens are important causes of morbidity in the United States, and considerable laboratory resources are spent to detect these enteric pathogens. This article reviews recent developments in the detection and identification of Campylobacter spp, enterohemorrhagic Escherichia co...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:
更新日期:1994-04-01 00:00:00
abstract::The Laboratory Proficiency Testing Program has been in effect for 20 years. During the past 6 years, samples were distributed for screening, investigation, and identification of hemoglobinopathies to test laboratory proficiency. Six samples for hemoglobin (Hb) S screening were distributed to from 37 to 163 laboratorie...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/107.5.567
更新日期:1997-05-01 00:00:00
abstract::The ideal method of heparinization should achieve therapeutic concentrations (0.2-0.5 IU/mL) in the artificial kidney and the least possible amount of heparin in the patient. Total heparinization using a bolus dose (8400 IU) followed by continuous infusion of heparin (20 IU/min), initially showed 1.4-2.4 IU/mL in the ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/84.3.317
更新日期:1985-09-01 00:00:00
abstract::Since the introduction of the triple screen, the emergence of additional analytes found to be associated with trisomy 21 pregnancies has challenged the clinical pathology and obstetric communities to determine which combination provides the best performance characteristics as a screening test. The cost, practicality, ...
journal_title:American journal of clinical pathology
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doi:10.1309/B7DBBLJW0W8P9G06
更新日期:2003-12-01 00:00:00
abstract:Objectives:Lymphoepithelioma-like carcinoma (LELC) of the uterine cervix is a rare tumor. The goal of this study was to evaluate a series of cases of cervical LELC and to investigate possible association with human papillomavirus (HPV) and/or Epstein-Barr virus (EBV). Methods:Immunohistochemistry for p63, p16, human l...
journal_title:American journal of clinical pathology
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更新日期:2019-01-07 00:00:00
abstract::During a 14-year period (1959-1972), 3,600 endometrial suction biopsies were performed in the office for specific indications in selected patients. Three hundred seventy-seven biopsies (10.5%) revealed abnormal findings. Of these, 233 showed endometritis, 96 associated with necrotic decidual or placental tissue, or bo...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:1975-01-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:2005-12-01 00:00:00
abstract::We evaluated a comprehensive deidentification engine at the University of Pittsburgh Medical Center (UPMC), Pittsburgh, PA, that uses a complex set of rules, dictionaries, pattern-matching algorithms, and the Unified Medical Language System to identify and replace identifying text in clinical reports while preserving ...
journal_title:American journal of clinical pathology
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更新日期:2004-02-01 00:00:00
abstract::High-performance liquid chromatography (HPLC) is replacing electrophoresis for identification of hemoglobin variants. Our objective was to identify unknown tall peaks with elution times and shapes of hemoglobin Barts found on hemoglobin chromatograms that could not be confirmed by alkaline and acid gel electrophoresis...
journal_title:American journal of clinical pathology
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更新日期:2006-04-01 00:00:00
abstract::The aim of this study was to evaluate the histopathologic features and the expression of angiogenesis-related markers in primary tumors and metastatic lymph nodes of oral squamous cell carcinomas (SCCs) with multiple lymph node involvement in comparison with oral SCCs without nodal metastasis. The protein levels of th...
journal_title:American journal of clinical pathology
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更新日期:2003-04-01 00:00:00
abstract::Chemotherapy is a well-established therapeutic approach for several malignancies, but its clinical efficacy is often limited by its related cardiotoxicity, which leads to cardiomyopathy, possibly evolving into heart failure. To detect cardiac damage, the adopted diagnostic approach is the estimation of left ventricula...
journal_title:American journal of clinical pathology
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更新日期:2008-11-01 00:00:00
abstract::Reduction mammaplasty (RM) is a common surgical procedure that yields a variable amount of tissue for pathologic examination. Occult breast carcinomas are detected rarely in these specimens. We evaluated the pathologic findings in RM specimens performed in our institution during an 11.5-year period (July 1989 to Decem...
journal_title:American journal of clinical pathology
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doi:10.1309/4kd652hn739xtlm3
更新日期:2003-09-01 00:00:00
abstract::Several monoclonal antibodies (MoAbs) are now available for immunophenotyping non-Hodgkin's lymphomas (NHLs) in paraffin-embedded tissue sections. To determine the reliability of these reagents in predicting the genotype, 44 cases of NHL were studied with the alkaline phosphatase-anti-alkaline phosphatase technique wi...
journal_title:American journal of clinical pathology
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更新日期:1991-04-01 00:00:00
abstract::Four acid hydrolases, acid phosphatase (AP), alpha-naphthyl acetate esterase (ANAE), beta-glucuronidase, and N-acetyl-beta-glucosaminidase, were determined cytochemically in peripheral blood lymphocytes from 50 patients with B and T chronic lymphocytic and prolymphocytic leukemias (CLL, PLL) and related disorders. Str...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/78.4.437
更新日期:1982-10-01 00:00:00
abstract::Hairy cell populations of greater than 90 purity were prepared from six samples obtained from four patients with leukemic reticuloendotheliosis (LRE). These then were analyzed for surface immunoglobulin (SIg) and antigens specified by a panel of monoclonal antibodies. The hairy cells from all patients displayed SIg an...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/79.4.431
更新日期:1983-04-01 00:00:00