Abstract:
:This study was aimed at examining the effect of tamoxifen, a selective estrogen receptor modulator, on the release of endogenous glutamate in rat cerebral cortex nerve terminals (synaptosomes) and exploring the possible mechanism. Tamoxifen inhibited the release of glutamate that was evoked by the K(+) channel blocker 4-aminopyridine (4-AP), and this phenomenon was concentration-dependent and insensitive to the estrogen receptor antagonist. The effect of tamoxifen on the evoked glutamate release was prevented by the chelating extracellular Ca(2+) ions, and by the vesicular transporter inhibitor bafilomycin A1. However, the glutamate transporter inhibitor dl-threo-beta-benzyloxyaspartate did not have any effect on the action of tamoxifen. Tamoxifen did not alter the resting synaptosomal membrane potential or 4-AP-mediated depolarization whereas it decreased the 4-AP-induced increase in cytosolic [Ca(2+)]. Furthermore, the inhibitory effect of tamoxifen on the evoked glutamate release was abolished by the Ca(v)2.2 (N-type) and Ca(v)2.1 (P/Q-type) channel blocker ω-conotoxin MVIIC, but not by the ryanodine receptor blocker dantrolene, or the mitochondrial Na(+)/Ca(2+) exchanger blocker CGP37157. In addition, the protein kinase C (PKC) inhibitors GF109203X or Ro318220 prevented tamoxifen from inhibiting glutamate release. Western blotting showed that tamoxifen significantly decreased the 4-AP-induced phosphorylation of PKC and PKCα. Together, these results suggest that tamoxifen inhibits glutamate release from rat cortical synaptosomes, through the suppression of presynaptic voltage-dependent Ca(2+) entry and PKC activity.
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Kuo JR,Wang CC,Huang SK,Wang SJdoi
10.1016/j.neuint.2011.11.014subject
Has Abstractpub_date
2012-01-01 00:00:00pages
105-14issue
2eissn
0197-0186issn
1872-9754pii
S0197-0186(11)00385-8journal_volume
60pub_type
杂志文章abstract::Mitochondrial respiratory chain (RC) disease is a heterogeneous and highly morbid group of energy deficiency disorders for which no proven effective therapies exist. Robust vertebrate animal models of primary RC dysfunction are needed to explore the effects of variation in RC disease subtypes, tissue-specific manifest...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2017.07.008
更新日期:2018-07-01 00:00:00
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journal_title:Neurochemistry international
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journal_title:Neurochemistry international
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journal_title:Neurochemistry international
pub_type: 杂志文章
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更新日期:1988-01-01 00:00:00
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journal_title:Neurochemistry international
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journal_title:Neurochemistry international
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journal_title:Neurochemistry international
pub_type: 杂志文章
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journal_title:Neurochemistry international
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更新日期:2019-12-01 00:00:00
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journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2011.05.006
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journal_title:Neurochemistry international
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Neurochemistry international
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journal_title:Neurochemistry international
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journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2014.05.012
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pub_type: 杂志文章
doi:10.1016/0197-0186(86)90157-9
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journal_title:Neurochemistry international
pub_type: 杂志文章,评审
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journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(01)00109-7
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journal_title:Neurochemistry international
pub_type: 杂志文章
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journal_title:Neurochemistry international
pub_type: 杂志文章
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journal_title:Neurochemistry international
pub_type: 杂志文章
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journal_title:Neurochemistry international
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journal_title:Neurochemistry international
pub_type: 杂志文章
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journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2004.03.005
更新日期:2004-10-01 00:00:00
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journal_title:Neurochemistry international
pub_type: 杂志文章
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更新日期:2006-06-01 00:00:00
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journal_title:Neurochemistry international
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