当前位置： SCI文献检索 > NEUROCHEMISTRY INTERNATIONAL期刊下所有文献 > [3H]dizocilpine binding to N-methyl-D-aspartate (NMDA) receptor is modulated by an endogenous Na+, K+-ATPase inhibitor. Comparison with ouabain.

[3H]dizocilpine binding to N-methyl-D-aspartate (NMDA) receptor is modulated by an endogenous Na+, K+-ATPase inhibitor. Comparison with ouabain.

Abstract：

:An endogenous Na+, K+-ATPase inhibitor termed endobain E has been isolated from rat brain which shares several biological properties with ouabain. This cardiac glycoside possesses neurotoxic properties attributable to Na+, K+-ATPase inhibition, which leads to NMDA receptor activation, thus supporting the concept that Na+/K+ gradient impairment has a critical impact on such receptor function. To evaluate potential direct effects of endobain E and ouabain on NMDA receptors, we assayed [3H]dizocilpine binding employing a system which excludes ionic gradient participation. Brain membranes thoroughly washed and stored as pellets ('non-resuspended' membranes) or after resuspension in sucrose ('resuspended' membranes) were employed. Membrane samples were incubated with 4 or 10 nM ligand with or without added endobain E or ouabain, in the presence of different glutamate plus glycine combinations, with or without spermidine. [3H]dizocilpine basal binding and Na+, K+- and Mg2+-ATPase activities proved very similar in 'non-resuspended' or 'resuspended' membranes. Endobain E decreased [3H]dizocilpine binding to 'resuspended' membranes in a concentration-dependent manner, attaining roughly 50% binding inhibition with the highest endobain E concentration assayed. Among tested conditions, only in 'resuspended' membranes, with 4 nM ligand and with 1x10(-8) M glutamate plus 1x10(-5) M glycine, was [3H]dizocilpine binding enhanced roughly +24% by ouabain (1 mM). After Triton X-100 membrane treatment, which drastically reduces Na+, K+-ATPase activity, the effect of ouabain on binding was lost whereas that of endobain E remained unaltered. Results indicate that not only membrane preparation but also treatment and storage are crucial to observe direct endobain E and ouabain effects on NMDA receptor, which are not attributable to changes in Na+, K+-ATPase activity or to Na+/K+ equilibrium alteration.

journal_name

Neurochem Int

journal_title

Neurochemistry international

authors

Reinés A,Peña C,Rodríguez de Lores Arnaiz G

doi

10.1016/s0197-0186(01)00034-1

subject

Has Abstract

pub_date

2001-10-01 00:00:00

pages

301-10

issue

4

eissn

0197-0186

issn

1872-9754

pii

S0197-0186(01)00034-1

journal_volume

39

pub_type

杂志文章

相关文献

NEUROCHEMISTRY INTERNATIONAL文献大全
  • Cocaine-induced status epilepticus and death generate oxidative stress in prefrontal cortex and striatum of mice.

    abstract：:Oxidative stress (OS) has been related to cocaine's actions and also to numerous nervous system pathologies, including seizures. The purpose of this work was to determine the alterations in glutathione (GSH) content, nitrite/nitrate and MDA levels after cocaine-induced toxicity. Male Swiss mice were injected (i.p.) wi...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2009.10.001

    authors： Macêdo DS,Vasconcelos SM,Andrade-Neto M,Belchior LD,Honório Júnior JE,Gonçalves DO,Fonteles MM,Silva MI,Aguiar LM,Viana GS,de Sousa FC

    更新日期：2010-01-01 00:00:00

  • Txnip mediates glucocorticoid-activated NLRP3 inflammatory signaling in mouse microglia.

    abstract：:Many studies indicate that chronic stress and excessive stress hormone can cause an inflammatory response. Thioredoxin-interacting protein (Txnip) as an endogenous thioredoxin inhibitor suppresses thioredoxin-produced antioxidant effects. Txnip was also found to interact with nucleotide-binding oligomerization domain-...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2019.104564

    authors： Bharti V,Tan H,Zhou H,Wang JF

    更新日期：2019-12-01 00:00:00

  • Release of acid-soluble radioactivity from rapidly transported proteins in homogenates of rabbit superior colliculus.

    abstract：:An intraocular injection of [(3)H]proline was used to label rabbit retinal ganglion cells including components subjected to axonal transport. Degradation of rapidly axonally transported labelled proteins was estimated in an in vitro system using homogenates of the nerve terminal regions. The energy requirements of thi...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(85)90061-0

    authors： Gustavsson S,Karlsson JO

    更新日期：1985-01-01 00:00:00

  • Human dihydrolipoamide dehydrogenase (E3) deficiency: Novel insights into the structural basis and molecular pathomechanism.

    abstract：:This review summarizes our present view on the molecular pathogenesis of human (h) E3-deficiency caused by a variety of genetic alterations with a special emphasis on the moonlighting biochemical phenomena related to the affected (dihydro)lipoamide dehydrogenase (LADH, E3, gene: dld), in particular the generation of r...

    journal_title：Neurochemistry international

    pub_type： 杂志文章,评审

    doi：10.1016/j.neuint.2017.05.018

    authors： Ambrus A,Adam-Vizi V

    更新日期：2018-07-01 00:00:00

  • Alpha-synuclein and its neurotoxic fragment inhibit dopamine uptake into rat striatal synaptosomes. Relationship to nitric oxide.

    abstract：:Alpha-synuclein (ASN), a 140-amino acid protein, is richly expressed in presynaptic terminals in the central nervous system, where it plays a role in synaptic vesicle function. However, if it is altered and accumulated it is involved in neurodegeneration as Parkinson's disease (PD). ASN contained 35-amino acid domain ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2006.01.025

    authors： Adamczyk A,Kaźmierczak A,Strosznajder JB

    更新日期：2006-09-01 00:00:00

  • Effects of typical (haloperidol) and atypical (risperidone) antipsychotic agents on protein expression in rat neural stem cells.

    abstract：:Neural stem cells (NSCs) play a crucial role in the development and maturation of the central nervous system. Recently studies suggest that antipsychotic drugs regulate the activities of NSCs. However, the molecular mechanisms underlying antipsychotic-induced changes of the activity of NSCs, particularly protein expre...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2009.05.007

    authors： Kashem MA,Ummehany R,Ukai W,Hashimoto E,Saito T,Mcgregor IS,Matsumoto I

    更新日期：2009-12-01 00:00:00

  • Aquaporin 4 antibody positive central nervous system autoimmunity and multiple sclerosis are characterized by a distinct profile of antibodies to herpes viruses.

    abstract：:Viral infections are implicated in the onset and promotion of autoimmunity in genetically predisposed individuals. In this study, immune response patterns to herpes viruses were compared in aquaporin 4 (AQP4) antibody positive central nervous system (CNS) autoimmunity and multiple sclerosis (MS). Serum samples of pati...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2010.08.003

    authors： Sellner J,Cepok S,Kalluri SR,Nestler A,Kleiter I,Kümpfel T,Linker R,Melms A,Menge T,Tumani H,Paul F,Hemmer B,Berthele A

    更新日期：2010-11-01 00:00:00

  • Effects of 3-hydroxy-4-amino-4,5,6,7-tetrahydro-1,2-benzisoxazol (exo-THPO) and its N-substituted analogs on GABA transport in cultured neurons and astrocytes and by the four cloned mouse GABA transporters.

    abstract：:The system of GABA transporters in neural cells constitutes an efficient mechanism for terminating inhibitory GABAergic neurotransmission. As such these transporter are important therapeutical targets in epilepsy and potentially other neurological diseases related to the GABA system. In this study a number of analogs ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(03)00033-0

    authors： Sarup A,Larsson OM,Bolvig T,Frølund B,Krogsgaard-Larsen P,Schousboe A

    更新日期：2003-09-01 00:00:00

  • Effects of ghrelin on the electrical activities of substantia nigra dopaminergic neurons treated with MPP.

    abstract：:Ghrelin, a 28 amino acid brain-gut peptide, has attracted increasing attention for its neuroprotective effect in Parkinson's disease (PD). In view of the pivotal role of excitability of dopaminergic neurons in substantia nigra pars compacta (SNc) in the function of nigrostriatal system, it is of great significance to ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2020.104780

    authors： Chang X,Ma Z,Shi L,Xie J

    更新日期：2020-09-01 00:00:00

  • In vitro neurotransmitter release in an animal model of depression.

    abstract：:Sprague-Dawley rats exposed to uncontrollable shock can be separated by a subsequent shock escape test into two groups: a "helpless" (LH) group which demonstrates a deficit in escape behavior, and a "nonlearned helpless" (NLH) group which shows no escape deficit and acquires the escape response as readily as naive con...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(92)90065-y

    authors： Edwards E,Kornrich W,van Houtten P,Henn FA

    更新日期：1992-07-01 00:00:00

  • Hemicholinum-3 binding sites in the nervous tissue of insects.

    abstract：:High-affinity binding sites for tritiated hemicholinium-3, a potent and selective inhibitor of the sodium-dependent high-affinity choline transport, have been found in synaptosomal membranes from insect ganglia. The binding of hemicholinium to ganglionic membrane preparations was saturable and specific with an apparen...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(89)90124-1

    authors： Knipper M,Krieger J,Breer H

    更新日期：1989-01-01 00:00:00

  • Uncompetitive stimulation of rat brain Na-K ATPase activity by rapid eye movement sleep deprivation.

    abstract：:Rapid eye movement sleep deprivation is associated with an increase in Na-K ATPase activity. In order to understand the possible biochemical mechanism of this increase, the kinetics of Na-K ATPase was studied. Although the enzyme activity increased after the deprivation, the catalytic efficiency of the enzyme remained...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(99)00121-7

    authors： Adya HV,Mallick BN

    更新日期：2000-03-01 00:00:00

  • Antidepressants attenuate the dexamethasone-induced decrease in viability and proliferation of human neuroblastoma SH-SY5Y cells: a involvement of extracellular regulated kinase (ERK1/2).

    abstract：:Excessive glucocorticoid levels in depressed patients have been associated with atrophic changes in some brain regions, but only few studies suggest that some antidepressants can interfere with deleterious effect of glucocorticoids on neuronal cells. The aim of the present study was to examine the effect of dexamethas...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2013.07.007

    authors： Leskiewicz M,Jantas D,Regulska M,Kaczanowska J,Basta-Kaim A,Budziszewska B,Kubera M,Lason W

    更新日期：2013-11-01 00:00:00

  • Effect of oxygen and glucose availability on the glycolytic rate in neuroblastoma cells under different conditions of culture.

    abstract：:Neuroblastoma cells, cultivated on plastic dishes, in presence of 15 mM glucose resist very well to hypoxia. Cells incubated on plastic dishes, if left unshaken, showed a Pasteur effect at an oxygen concentration below 10%. Oxygen diffusion was the limiting factor in these plastic dishes since improved oxygen diffusio...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(84)90116-5

    authors： Pauwels PJ,Opperdoes FR,Trouet A

    更新日期：1984-01-01 00:00:00

  • Changes in gene expression of AMPA-selective glutamate receptor subunits induced by status epilepticus in rat brain.

    abstract：:In the present investigation we address the question of whether one of the responses to increased neuronal activity is a modification of the expression of the different subunits of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA)-selective glutamate receptors (GluR-1, GluR-2, GluR-3). Thus, we used two dif...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(94)90144-9

    authors： Condorelli DF,Belluardo N,Mudò G,Dell'Albani P,Jiang X,Giuffrida-Stella AM

    更新日期：1994-10-01 00:00:00

  • Mechanism of the beneficial effect of melatonin in experimental Parkinson's disease.

    abstract：:This study aimed to elucidate locomotor activity changes in 6-hydroxydopamine (6-OHDA) induced Parkinson's disease (PD) and investigate the possible beneficial effects of melatonin on altered levels of locomotor activity, cyclooxygenase (COX), prostaglandin E2 (PGE2), nuclear factor kappa-B (NF-κB), nitrate/nitrite an...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2014.09.005

    authors： Yildirim FB,Ozsoy O,Tanriover G,Kaya Y,Ogut E,Gemici B,Dilmac S,Ozkan A,Agar A,Aslan M

    更新日期：2014-12-01 00:00:00

  • Glutamate-responsive translation of dendritic GSK3β mRNA triggers a cycle for amplification of reactivated preexisting GSK3β that is indispensable for tau hyperphosphorylation.

    abstract：:The molecular mechanism responsible for hyperphosphorylated tau accumulation in dendrites of Alzheimer's disease (AD) neurons has not been fully clarified. Recently, we reported that tau mRNA is distributed into dendrites, and that translation and phosphorylation of tau protein are immediately enhanced in response to ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2020.104808

    authors： Tanaka T,Ohashi S,Takashima A,Kobayashi S

    更新日期：2020-10-01 00:00:00

  • Glutaminase activity in GLS1 Het mouse brain compared to putative pharmacological inhibition by ebselen using ex vivo MRS.

    abstract：:Glutaminase mediates the recycling of neurotransmitter glutamate, supporting most excitatory neurotransmission in the mammalian central nervous system. A constitutive heterozygous reduction in GLS1 engenders in mice a model of schizophrenia resilience and associated increases in Gln, reductions in Glu and activity-dep...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2019.104508

    authors： Kosten L,Chowdhury GMI,Mingote S,Staelens S,Rothman DL,Behar KL,Rayport S

    更新日期：2019-10-01 00:00:00

  • Connexins in oligodendrocytes and astrocytes: Possible factors for demyelination in multiple sclerosis.

    abstract：:Increasing evidences support that glial connexins are involved in the demyelination pathology of multiple sclerosis (MS), a chronic inflammatory demyelinating disorder. Here, we review the data from patients with MS and animal models of MS that implicate connexins in demyelination. Connexins expressed in oligodendrocy...

    journal_title：Neurochemistry international

    pub_type： 杂志文章,评审

    doi：10.1016/j.neuint.2020.104731

    authors： Xia CY,Xu JK,Pan CH,Lian WW,Yan Y,Ma BZ,He J,Zhang WK

    更新日期：2020-06-01 00:00:00

  • Chronic citalopram treatment elevates serotonin synthesis in flinders sensitive and flinders resistant lines of rats, with no significant effect on Sprague-Dawley rats.

    abstract：:The influence of citalopram on regional 5-hydroxytryptamine (serotonin, 5-HT) synthesis, one of the most important presynaptic parameters of serotonergic neurotransmission, was studied. Sprague-Dawley (SPD) rats were used as the controls, and Flinders Resistant Line (FRL) rats were used as auxiliary controls, to hopef...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2009.01.005

    authors： Kanemaru K,Nishi K,Hasegawa S,Diksic M

    更新日期：2009-05-01 00:00:00

  • Changes in iron-regulatory gene expression occur in human cell culture models of Parkinson's disease.

    abstract：BACKGROUND:Neuronal iron accumulation is thought to be relevant to the pathogenesis of Parkinson's disease (PD), although the mechanism remains elusive. We hypothesized that neuronal iron uptake may be stimulated by functional mitochondrial iron deficiency. OBJECTIVE:To determine firstly whether the mitochondrial toxi...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2011.05.006

    authors： Carroll CB,Zeissler ML,Chadborn N,Gibson K,Williams G,Zajicek JP,Morrison KE,Hanemann CO

    更新日期：2011-08-01 00:00:00

  • Cerebrospinal fluid light and heavy neurofilaments in neuromyelitis optica.

    abstract：BACKGROUND:Axonal injury is the correlate of disease progression in NMO and MS. Neurofilament (Nf) belongs to neuron specific intermediate filaments located in axons. Nf protein subunits are potential biomarkers in cerebrospinal fluid (CSF) for acute axonal injury. However, whether CSF NfH and NfL levels are elevated i...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2013.10.008

    authors： Wang H,Wang C,Qiu W,Lu Z,Hu X,Wang K

    更新日期：2013-12-01 00:00:00

  • 6-Hydroxydopamine induces cystatin C-mediated cysteine protease suppression and cathepsin D activation.

    abstract：:Alteration in the lysosomal system (LS) may represent a central mechanism in neurodegeneration. 6-Hydroxydopamine (6-OHDA) induces oxidative stress and cell death in catecholaminergic cells. The LS and caspases participate in apoptosis, although the mechanism(s) that is involved is not completely understood. Here, we ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2006.12.006

    authors： Lee DC,Womble TA,Mason CW,Jackson IM,Lamango NS,Severs WB,Palm DE

    更新日期：2007-03-01 00:00:00

  • The in vivo modulation of dopamine synthesis by calcium ions: influences on the calcium independent release.

    abstract：:To investigate the contribution of the dopamine (DA) synthesis to both the calcium-dependent and the carrier-mediated, mechanisms of DA release in the striatum, anaesthetized rats were locally superfused in the striatum with a push pull cannula supplied with an artificial CSF containing tritiated tyrosine. DA, dihydro...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(99)00094-7

    authors： Olivier V,Gobert A,Guibert B,Leviel V

    更新日期：1999-12-01 00:00:00

  • Chronic ethanol exposure enhances AMPA-elicited Ca2+ signals in the somatic and dendritic regions of cerebellar Purkinje neurons.

    abstract：:Intracellular Ca2+ signals produced by the glutamate receptor agonist alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA; 5 microM) were measured in the somatic and dendritic regions of cerebellar Purkinje neurons in mature cerebellar control cultures (> or = 20 days in vitro) and cultures chronically treated...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(99)00058-3

    authors： Netzeband JG,Trotter C,Caguioa JN,Gruol DL

    更新日期：1999-08-01 00:00:00

  • The regulation of corticosteroid receptors in response to chronic social defeat.

    abstract：:Our previous studies demonstrated that chronic social defeat (CSD) up-regulated expression of the serotonin transporter (SERT) and norepinephrine transporter (NET) in the brain, which was mediated by corticosteroid receptors. In the present study we first analyzed the alterations of corticosteroid receptors in differe...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2017.05.021

    authors： Zhang J,Fan Y,Raza MU,Zhan Y,Du XD,Patel PD,Zhu MY

    更新日期：2017-09-01 00:00:00

  • Regulation of intracellular [Ca2+] and GABA release by presynaptic GABAB receptors in rat cerebrocortical synaptosomes.

    abstract：:In this study we determined the changes in the intracellular free Ca2+ concentration, associated with the inhibitory modulation of the exocytotic release of GABA by GABAB receptor activation in rat cerebrocortical synaptosomes. We observed that SK&F 97541 and (-)baclofen both act as agonists of the presynaptic GABAB r...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(95)00021-y

    authors： Santos AE,Carvalho CM,Macedo TA,Carvalho AP

    更新日期：1995-10-01 00:00:00

  • Acute ethanol administration induces changes in TRH and proenkephalin expression in hypothalamic and limbic regions of rat brain.

    abstract：:Thyrotropin releasing hormone (TRH) present in several brain areas has been proposed as a neuromodulator. Its administration produces opposite effects to those observed with acute ethanol consumption. Opioid peptides, in contrast, have been proposed to mediate some of the effects of alcohol intoxication. We measured T...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(00)00059-0

    authors： de Gortari P,Méndez M,Rodríguez-Keller I,Pérez-Martínez L,Joseph-Bravob P

    更新日期：2000-11-01 00:00:00

  • The protective effect of eicosapentaenoic acid-enriched phospholipids from sea cucumber Cucumaria frondosa on oxidative stress in PC12 cells and SAMP8 mice.

    abstract：:Alzheimer's disease (AD) is a common neurodegenerative disorders, in which oxidative stress plays an important role. The present study investigated the effect of eicosapentaenoic acid-enriched phospholipids (EPA-enriched PL) from the sea cucumber Cucumaria frondosa on oxidative injury in PC12 cells induced by hydrogen...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2013.10.015

    authors： Wu FJ,Xue Y,Liu XF,Xue CH,Wang JF,Du L,Takahashi K,Wang YM

    更新日期：2014-01-01 00:00:00

  • Degradation of human atrial natriuretic peptide by human brain membranes.

    abstract：:Human atrial natriuretic peptide (Ser 99-Tyr 126) was rapidly degraded by both choroid plexus and hypothalamic membranes with a complex pattern of cleavage. The use of protease inhibitors allowed a preliminary characterization of the enzymes involved in the hydrolysis of the Ser-Phe and Phe-Arg bonds of iodine-labelle...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(88)90176-3

    authors： Deschodt-Lanckman M,Vanneste Y,Michaux F

    更新日期：1988-01-01 00:00:00