Degradation of human atrial natriuretic peptide by human brain membranes.


:Human atrial natriuretic peptide (Ser 99-Tyr 126) was rapidly degraded by both choroid plexus and hypothalamic membranes with a complex pattern of cleavage. The use of protease inhibitors allowed a preliminary characterization of the enzymes involved in the hydrolysis of the Ser-Phe and Phe-Arg bonds of iodine-labelled atrial natriuretic peptide. The C-terminal tripeptide was generated by three different enzymatic activities acting on the Ser-Phe bond: endopeptidase 24.11, a phosphoramidon-insensitive metallopeptidase and a thiol protease. Peptides like substance P, neurotensin, bradykinin inhibited the cleavage of the Ser-Phe bond of atrial natriuretic peptide. The C-terminal tripeptide was further degraded by aminopeptidases. Cleavage of the C-terminal dipeptide was inhibited by aprotinin, suggesting the contribution of brain kallikrein in the formation of this metabolite. These results show that many different proteases were involved in the hydrolysis of the C-terminal sequence of atrial natriuretic peptide, at least in vitro and underline the complexity of neuropeptide catabolism by brain preparations.


Neurochem Int


Deschodt-Lanckman M,Vanneste Y,Michaux F




Has Abstract


1988-01-01 00:00:00














  • Effects of imidazolines on noradrenaline release in brain: an investigation into their relationship to imidazoline, alpha 2 and H3 receptors.

    abstract::The present study was carried out to clarify whether the imidazolines clonidine, moxonidine and cirazoline as well as the guanidine aganodine inhibit noradrenaline release in the rat and rabbit brain via imidazoline receptors, alpha 2-adrenoceptors and/or histamine H3 receptors. Slices or synaptosomes from the rat or ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Schlicker E,Fink K,Kathmann M,Molderings GJ,Göthert M

    更新日期:1997-01-01 00:00:00

  • 6-Hydroxydopamine induces cystatin C-mediated cysteine protease suppression and cathepsin D activation.

    abstract::Alteration in the lysosomal system (LS) may represent a central mechanism in neurodegeneration. 6-Hydroxydopamine (6-OHDA) induces oxidative stress and cell death in catecholaminergic cells. The LS and caspases participate in apoptosis, although the mechanism(s) that is involved is not completely understood. Here, we ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Lee DC,Womble TA,Mason CW,Jackson IM,Lamango NS,Severs WB,Palm DE

    更新日期:2007-03-01 00:00:00

  • Altered precursor availability and metabolism of monoamines in the brain caused by the injection of physiologic saline to suckling rats: On the reliability of saline "controls" in neurochemical studies.

    abstract::The effect of subcutaneous injections of saline (0.9% NaCl, 10-40 ?l/g b. wt) to 5- and 20-day old rats on the concentrations of tyrosine (Tyr) and tryptophan (Trp) in the serum and the brain and on the levels of biogenic amines and their metabolites in the developing brain at 6 h p.i. is described. At day 5 the conce...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Huether G,Thoemke F,Sprotte U,Steinhaus U,Neuhoff V

    更新日期:1985-01-01 00:00:00

  • The relation between respiratory inhibition and uptake of 1-methyl-isoquinoline (MIQ+) in mitochondria.

    abstract::The effect of 1-methyl-isoquinoline (MIQ+) on the respiratory inhibition and the uptake of MIQ+ were measured using mouse liver mitochondria. MIQ+ inhibited the electron transport of complex I but did not inhibit the respiration of mitochondria with succinate as a substrate. MIQ+ was taken up by mitochondria in an ene...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Aiuchi T,Matsunaga M,Syo M,Nakaya K

    更新日期:1996-03-01 00:00:00

  • A glutamatergic component of lead toxicity in adult brain: the role of astrocytic glutamate transporters.

    abstract::Astroglial cells have a variety of roles in the central nervous system (CNS), providing a homeostasis for the proper functioning of neuronal cells. The classical view concerning the supportive role of astroglia towards associated neurons has to be extended. A great number of new evidences suggest that astrocytes inter...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审


    authors: Struzyńska L

    更新日期:2009-07-01 00:00:00

  • GABA release and uptake measured in crude synaptosomes from Genetic Absence Epilepsy Rats from Strasbourg (GAERS).

    abstract::GABA release and uptake were examined in Genetic Absence Epilepsy Rats from Strasbourg and in non-epileptic control animals, using crude synaptosomes prepared from the cerebral cortex and thalamus. Uptake of [3H]GABA over time was reduced in thalamic synaptosomes from epileptic rats, compared to controls. The affinity...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Sutch RJ,Davies CC,Bowery NG

    更新日期:1999-05-01 00:00:00

  • Induction of conditioned place preference and dopamine release by salsolinol in posterior VTA of rats: involvement of μ-opioid receptors.

    abstract::Salsolinol (Sal), locally administered into the posterior VTA (pVTA) of rats, produces psychomotor responses and reinforcing effects, probably, through the activation of μ-opioid receptors (MORs). The neurochemical correlates of these phenomena are, however, practically unknown. In this paper, we explore the neurochem...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Hipólito L,Martí-Prats L,Sánchez-Catalán MJ,Polache A,Granero L

    更新日期:2011-10-01 00:00:00

  • Postnatal functional inactivation of the ventral subiculum enhances dopaminergic responses in the core part of the nucleus accumbens following ketamine injection in adult rats.

    abstract::For almost two decades schizophrenia has been considered to be a functional disconnection disorder. This functional disconnectivity between several brain regions could have a neurodevelopmental origin. Various approaches suggest the ventral subiculum (SUB) is a particular target region for neurodevelopemental disturba...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Saoud H,De Beus D,Eybrard S,Louilot A

    更新日期:2020-07-01 00:00:00

  • Phospholipase A2 products retain a neuron specific gamma isoform of PKC on the plasma membrane through the C1 domain--a molecular mechanism for sustained enzyme activity.

    abstract::To clarify molecular mechanism for sustained activation of gamma protein kinase C (gammaPKC), a neuron-specific subtype, we investigated the involvement of phospholipase A2 (PLA2) products in the membrane association of gammaPKC upon activation of G protein coupled purinoceptors in CHO-K1 and NG 108-15 cells. In addit...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Yagi K,Shirai Y,Hirai M,Sakai N,Saito N

    更新日期:2004-07-01 00:00:00

  • The adenosine A2A receptor antagonist SCH58261 reduces macrophage/microglia activation and protects against experimental autoimmune encephalomyelitis in mice.

    abstract::Multiple sclerosis (MS) is a chronic autoimmune inflammatory disease of the central nervous system (CNS) affecting more than 2.5 million individuals worldwide. In the present study, myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE) mice were treated with adenosine recept...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Chen Y,Zhang ZX,Zheng LP,Wang L,Liu YF,Yin WY,Chen YY,Wang XS,Hou ST,Chen JF,Zheng RY

    更新日期:2019-10-01 00:00:00

  • The pentylenetetrazole-kindling model of epilepsy in SAMP8 mice: glial-neuronal metabolic interactions.

    abstract::Recently, a new experimental model of epilepsy was introduced by the authors [Neurochem. Int. 40 (2002) 413]. This model combines pentylenetetrazole (PTZ)-kindling in senescence-accelerated mice P8 (SAMP8), a genetic model of aging. Since imbalance of glutamate and GABA is a major cause of seizures, the study of glial...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Kondziella D,Hammer J,Sletvold O,Sonnewald U

    更新日期:2003-12-01 00:00:00

  • Effects of ghrelin on the electrical activities of substantia nigra dopaminergic neurons treated with MPP.

    abstract::Ghrelin, a 28 amino acid brain-gut peptide, has attracted increasing attention for its neuroprotective effect in Parkinson's disease (PD). In view of the pivotal role of excitability of dopaminergic neurons in substantia nigra pars compacta (SNc) in the function of nigrostriatal system, it is of great significance to ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Chang X,Ma Z,Shi L,Xie J

    更新日期:2020-09-01 00:00:00

  • Comparison of [(3)H]-(2S,4R)-4-methylglutamate and [(3)H]D-aspartate as ligands for binding and autoradiographic analyses of glutamate transporters.

    abstract::While studies with [(3)H]D-aspartate ([(3)H]d-Asp) illustrate specific interactions with excitatory amino acid transporters (EAATs), new insights into the pharmacological characteristics and localization of specific EAAT subtypes depend upon the availability of novel ligands. One such ligand is [(3)H]-(2S,4R)-4-methyl...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Apricò K,Beart PM,Crawford D,O'shea RD

    更新日期:2007-12-01 00:00:00

  • Purification and partial characterization of K+ channel blockers from the venom of Dendroaspis angusticeps.

    abstract::Two polypeptides (designated DTX-A and DTX-B) were purified from crude snake venom of Dendroaspis angusticeps using gel filtration, cation exchange column chromatography and cation exchange high performance liquid chromatography, and their blocking actions of K+ channels were investigated in rat brain synaptosomes. Bo...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Chaki S,Muramatsu M,Ushiyama Y,Otomo S

    更新日期:1992-06-01 00:00:00

  • Neurotensin concentrations in rat striatum and nucleus accumbens: Further studies of their regulation.

    abstract::The possible influence of cholinergic and dopaminergic mechanisms on neurotensin-containing neurones was examined at two different levels; nucleus accumbens and striatum in the rat brain. The acute treatment with the anticholinergic drugs atropine and scopolamine increased neurotensin concentrations in the striatum an...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Frey P,Lis M,Coward DM

    更新日期:1988-01-01 00:00:00

  • Methamphetamine use causes cognitive impairment and altered decision-making.

    abstract::Methamphetamine is a widely abused psychostimulant. It reverses transport through the dopamine transporter, thereby increasing the extracellular level of dopamine in the brain, which is associated with the rewarding effect. Repeated intake of methamphetamine leads to drug addiction, a chronically relapsing disorder ch...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审


    authors: Mizoguchi H,Yamada K

    更新日期:2019-03-01 00:00:00

  • Extrasynaptic GABAA receptors in the crosshairs of hormones and ethanol.

    abstract::Gamma-aminobutyric acid (GABA) is the main chemical inhibitory neurotransmitter in the brain. In the central nervous system (CNS) it acts on two distinct types of receptor: an ion channel, i.e., an "ionotropic" receptor permeable to Cl- and HCO3- (GABAA receptors) and a G-protein coupled "metabotropic" receptor that i...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审


    authors: Mody I

    更新日期:2008-01-01 00:00:00

  • Long-term changes in noradrenaline (NA) and dopamine (DA) contents of rat cerebellum following neonatal X-irradiation.

    abstract::The effects of neonatal X-irradiation on cerebellar cathecholamine levels in rats were studied at different postnatal intervals. Since synaptogenesis in the cerebellar cortex is basically a postnatal phenomenon, changes in noradrenaline (NA) and dopamine (DA) levels induced by X-rays on the cerebella (CE) of adult rat...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Dopico AM,Zieher LM,Mayo J,Altschüller N,López JJ,Jaim-Etcheverry G

    更新日期:1989-01-01 00:00:00

  • alpha(2)-Adrenoceptor subtypes-mediated physiological, pharmacological actions.

    abstract::alpha(2)-Adrenoceptors are involved in various physiological functions, particularly in the cardiovascular system and the central nervous system. Different adrenoceptor subtypes (alpha(2A), alpha(2B) and alpha(2C)) have been recognised and the different subtypes may have role in activation of distinct physiological an...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审


    authors: Gyires K,Zádori ZS,Török T,Mátyus P

    更新日期:2009-12-01 00:00:00

  • Effects of Vinpocetine on mitochondrial function and neuroprotection in primary cortical neurons.

    abstract::Vinpocetine (ethyl apovincaminate), a synthetic derivative of the Vinca minor alkaloid vincamine, is widely used for the treatment of cerebrovascular-related diseases. One of the proposed mechanisms underlying its action is to protect against the cytotoxic effects of glutamate overexposure. Glutamate excitotoxicity le...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Tárnok K,Kiss E,Luiten PG,Nyakas C,Tihanyi K,Schlett K,Eisel UL

    更新日期:2008-12-01 00:00:00

  • Cholinergic and purinergic signalling in outer hair cells of mammalian cochlea.

    abstract::Outer (OHC) and inner (IHC) hair cells in the organ of Corti of the mammalian cochlea process sound. OHC and their efferent synapse are part of a feedback system assumed to control and modulate information carried by afferent neurons passing from IHC to the brain. Underlying mechanisms are not well understood. This pa...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审


    authors: Heilbronn E,Järlebark L,Lawoko G

    更新日期:1995-10-01 00:00:00

  • Electromagnetic fields induce neural differentiation of human bone marrow derived mesenchymal stem cells via ROS mediated EGFR activation.

    abstract::Even though the inducing effect of electromagnetic fields (EMF) on the neural differentiation of human bone marrow mesenchymal stem cells (hBM-MSCs) is a distinctive, the underlying mechanism of differentiation remains unclear. To find out the signaling pathways involved in the neural differentiation of BM-MSCs by EMF...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Park JE,Seo YK,Yoon HH,Kim CW,Park JK,Jeon S

    更新日期:2013-03-01 00:00:00

  • An in vitro model for the study of microglia-induced neurodegeneration: involvement of nitric oxide and tumor necrosis factor-alpha.

    abstract::The precise function of activated microglia and their secretory products remains controversial. In order to assess the role of microglial secretion products, we established an in vitro model of an inflammatory reaction in the brain by co-culturing microglial and neuronal cell lines. Upon stimulation with interferon-ga...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Hemmer K,Fransen L,Vanderstichele H,Vanmechelen E,Heuschling P

    更新日期:2001-06-01 00:00:00

  • Regional increases in ornithine decarboxylase mRNA levels in the rat brain after partial mesodiencephalic hemitransection as revealed by in situ hybridization histochemistry.

    abstract::Changes in the level of ornithine decarboxylase mRNA after partial mesodiencephalic hemitransection were evaluated in various regions of the rat brain by means of in situ hybridization histochemistry coupled with computer-assisted image analysis. On days 1 and 2 after the lesion, increased accumulation of ornithine de...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Zoli M,Bettuzzi S,Ferraguti F,Ingletti MC,Zini I,Fuxe K,Agnati LF,Corti A

    更新日期:1991-01-01 00:00:00

  • Maximal GABA and muscimol binding to high-affinity sites differ in physiological and in non-physiological buffers.

    abstract::The sensitivity of [(3)H]GABA and [(3)H]muscimol high-affinity binding sites to physiological (Krebs-Ringer's bicarbonate) and non-physiological (Tris-citrate) buffers was examined using synaptosomal membranes from bovine retinas. The maximum number of sites (B(max)) for [(3)H]GABA was present when the tissue was assa...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Madtes P Jr,Bashir-Elahi R,Chader GJ

    更新日期:1986-01-01 00:00:00

  • Adult development of the hippocampal-serotonin system of C57BL/6N mice; analysis of high-affinity uptake of (3)H-5HT in slices and synaptosomes.

    abstract::The saturable and specific high-affinity uptake of [(3)H]serotonin ([(3)H]5HT) (5 x 10(?8) M) was studied in slices from the hippocampus, parietal cortex, septum-preoptic area, and hypothalamus of male 2, 6, 12 and 24-32 month old C57BL/6N mice. Hippocampal [(3)H]5-HT uptake showed a significant biphasic relationship ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Azmitia E,Brennan MJ,Quartermain D

    更新日期:1983-01-01 00:00:00

  • Kinin-mediated inflammation in neurodegenerative disorders.

    abstract::The mediatory role of kinins in both acute and chronic inflammation within nervous tissues has been widely described. Bradykinin, the major representative of these bioactive peptides, is one of a few mediators of inflammation that directly stimulates afferent nerves due to the broad expression of specific kinin recept...

    journal_title:Neurochemistry international

    pub_type: 杂志文章,评审


    authors: Guevara-Lora I

    更新日期:2012-07-01 00:00:00

  • 1-Methyl-4-phenylpyridinium (MPP+)-induced cell death in PC12 cells: inhibitory effects of several drugs.

    abstract::1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), an inducer of parkinsonism, causes degeneration of nigro-striatal dopaminergic neurons by producing its neurotoxic metabolite, 1-methyl-4-phenylpiridium ion (MPP+), by monoamine oxidase B in glial cells. We used PC12 (rat pheochromocytoma cell line) as a model cell ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Itano Y,Kitamura Y,Nomura Y

    更新日期:1994-11-01 00:00:00

  • Correlation between rotational behaviors and neurochemical changes associated with damage of rat striatum: Analysis using unilateral microinjection of kainic acid.

    abstract::The correlation between rotational behaviors and neurochemical changes associated with the striatal damage induced by an unilateral microinjection of kainic acid were investigated. Shortly after the unilateral striatal injection of kainic acid, rats exhibited contralateral rotational behaviors, and these changes were ...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Kuriyama K,Kurihara E

    更新日期:1982-01-01 00:00:00

  • Evidence of sexual dimorphism in D1 and D2 dopaminergic receptors expression in frontal cortex and striatum of young rats.

    abstract::D1 and D2 receptors are key mediators of dopaminergic signaling in the brain, and since the manifestations of pathologies related to dopamine are different in female and male patients, it is important to analyze if there are sex-related differences in dopaminergic markers. To contribute to the knowledge in this regard...

    journal_title:Neurochemistry international

    pub_type: 杂志文章


    authors: Orendain-Jaime EN,Ortega-Ibarra JM,López-Pérez SJ

    更新日期:2016-11-01 00:00:00