Abstract：
:RBFox proteins are well-known alternative splicing regulators. We have shown previously that during neuronal differentiation of P19 cells induced by all-trans retinoic acid and cell aggregation, RBFox1 shows markedly increased temporal expression. To find its key splicing regulation, we examined the effect of RBFox1 on 33 previously reported and validated neuronal splicing events of P19 cells. We observed that alternative splicing of three genes, specifically, amyloid precursor protein (APP), disks large homolog 3 (DLG3), and G protein, alpha activating activity polypeptide O (GNAO1), was altered by transient RBFox1 expression in HEK293 and HeLa cells. Moreover, an RBFox1 mutant (RBFox1FA) that was unable to bind the target RNA sequence ((U)GCAUG) did not induce these splicing events. APP generates amyloid beta peptides that are involved in the pathology of Alzheimer's disease, and therefore we examined APP alternative splicing regulation by RBFox1 and other splicing regulators. Our results indicated that RBFox proteins promote the skipping of APP exon 7, but not the inclusion of exon 8. We made APP6789 minigenes and observed that two (U)GCAUG sequences, located upstream of exon 7 and in exon 7, functioned to induce skipping of exon 7 by RBFox proteins. Overall, RBFox proteins may shift APP from exon 7 containing isoforms, APP770 and APP751, toward the exon 7 lacking isoform, APP695, which is predominant in neural tissues.
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Alam S,Suzuki H,Tsukahara Tdoi
10.1016/j.neuint.2014.08.001subject
Has Abstractpub_date
2014-12-01 00:00:00pages
7-17eissn
0197-0186issn
1872-9754pii
S0197-0186(14)00184-3journal_volume
78pub_type
杂志文章abstract：:The present study was aimed to investigate the effects of a chronic treatment with the dopamine uptake blocker nomifensine on the in vivo extracellular concentrations of dopamine, acetylcholine, glutamate and GABA in the prefrontal cortex, striatum and nucleus accumbens. Male Wistar rats received intraperitoneal (i.p....
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2007.08.005
更新日期：2008-02-01 00:00:00
abstract：:Acetylcholinesterase release in the guinea-pig substantia nigra has been previously investigated 'on-line', using a sensitive chemiluminescent system. Since histological observations suggest that there is a difference in acetylcholinesterase distribution in the rat substantia nigra compared to that of the guinea-pig, ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(96)00057-5
更新日期：1996-12-01 00:00:00
abstract：:It was shown previously that pure oligodendrocytes release proteins when maintained in a chemically defined medium. Among these proteins, a 53 kDa glycoprotein was characterized as a component accessible from the external surface of these glial cells. Specific antibodies directed against this glycoprotein were obtaine...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(88)90101-5
更新日期：1988-01-01 00:00:00
abstract：:TAR DNA-binding protein 43 (TDP-43) is a major pathological protein of ubiquitinated inclusions in motor neurons of sporadic amyotrophic lateral sclerosis (ALS). TDP-43 is ubiquitously expressed and the majority of TDP-43 is normally localized to the nucleus. In motor neurons of patients with ALS, TDP-43 is not locali...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2018.12.009
更新日期：2019-03-01 00:00:00
abstract：:In this report evidence is presented for a membrane-associated polypeptide that regulates ligand binding properties of a neuronal acetylcholine receptor. Removal of membrane-associated compounds reversibly increased the number of BGTX binding sites and decreased the number of binding sites for ACh in neuronal membrane...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(90)90104-2
更新日期：1990-01-01 00:00:00
abstract：:The labelling of gangliosides and sialoglycoproteins (SGP) after injecting intraocularly 30 ?Ci of [(3)H]ManNAc was studied in chickens that had previously received, also intraocularly, 30 or 70 nmol of monensin in 10 ?l of ethanol or 24 ?g of colchicine in 10 ?l saline. Controls received ethanol or saline only. Colch...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(86)90183-x
更新日期：1986-01-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(01)00102-4
更新日期：2002-04-01 00:00:00
abstract：:The discovery of nonsynaptic communication in the 1960s and 1970s was an important milestone in investigating the function of the nervous system, and it revolutionized our view about information transmission between neurons. In addition, nonsynaptic communication has a practical importance not only within the nervous ...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/j.neuint.2007.06.001
更新日期：2008-01-01 00:00:00
abstract：:D1 dopamine receptors have been localized using a radioactive form of a new specific antagonist, [3H]SCH39166. This compound has been shown, in in vitro binding studies, to be highly selective for the D1 receptor subtype; more so than its predecessor, [3H]SCH23390. These ligand binds saturably, reversibly and with hig...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
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更新日期：1992-03-01 00:00:00
abstract：:Control of serotonin release and synthesis by amino acid neurotransmitters was investigated in rat rostral and caudal rhombencephalic raphe cells in primary cultures respectively. Endogenous amounts of taurine, glycine, GABA and glutamate were measured in both types of cultures. These amino acids were spontaneously re...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(93)90118-o
更新日期：1993-09-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2013.06.017
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2010.10.001
更新日期：2010-12-01 00:00:00
abstract：:Multiple sclerosis (MS) is an autoimmune disease in which pathogenic T cells play an important role, and an experimental autoimmune encephalomyelitis (EAE) is used as an animal model of MS. Galectins are β-galactoside-binding lectins and involved in various physiological and pathological events. Among fifteen members ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2018.06.007
更新日期：2018-09-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(85)90061-0
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2013.10.015
更新日期：2014-01-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2019.03.006
更新日期：2019-06-01 00:00:00
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journal_title：Neurochemistry international
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abstract：:The onset of attention-deficit-hyperactivity-disorder (ADHD) in childhood is characterized by developmentally inappropriate levels of hyperactivity, impulsivity and inattention. A chronic deficit of serotonin (5-HT) at the synapse may trigger symptoms of ADHD. This review focuses on neuro-anatomical, experimental and ...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2007.04.032
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(88)90007-1
更新日期：1988-01-01 00:00:00
abstract：:Nitrite therapy is more effective in cerebral ischemia when administered earlier. It would be beneficial during the hyperacute stages of stroke if the nitrite effect is demonstrated in intracerebral hemorrhage (ICH). When nitrite is injected intravenously 3h after ICH induction in rats, most doses of nitrite provided ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2011.05.018
更新日期：2011-08-01 00:00:00
abstract：:Previously we reported that gene expression of astrocytic 5-HT2B receptors was decreased in brains of depressed animals exposed to chronic mild stress (CMS) (Li et al., 2012) and of Parkinson's disease (Song et al., 2018). Depression is also one of the psychiatric symptoms in hyperammonemia, and astrocyte is a primary...
journal_title：Neurochemistry international
pub_type： 杂志文章
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更新日期：2019-10-01 00:00:00
abstract：:Much attention has been paid to the connection between periodontitis and Alzheimer's disease (AD). We previously showed that infection of P. gingivalis, one of major periodontal pathogen causing periodontitis, induced migration and inflammatory responses in murine microglia through gingipain-induced activation of prot...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2020.104840
更新日期：2020-11-01 00:00:00
abstract：:Toluene is a commonly abused solvent found in many industrial and commercial products. The neurobiological effects of toluene remain unclear, but many of them, like those of ethanol, may be mediated by gamma-aminobutyric acid (GABA) and glutamate receptors. Chronic ethanol administration has been shown to alter levels...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2004.07.006
更新日期：2005-01-01 00:00:00
abstract：:Brain edema and the subsequent increase in intracranial pressure are the major neurological complications in fulminant hepatic failure (FHF). Brain edema in FHF is predominantly "cytotoxic" due principally to astrocyte swelling. It is generally believed that ammonia plays a key role in this process, although the mecha...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2005.11.017
更新日期：2006-05-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
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更新日期：1994-05-01 00:00:00
abstract：:The activity of histochemically demonstrable aspartate aminotransferase was measured histophotometrically in the hippocampal formation, in dorsal root ganglia, and in superior cervical ganglia during postnatal development of the rat. The enzyme activity showed a marked (up to 15 times) increase in the dendritic layers...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(87)90146-x
更新日期：1987-01-01 00:00:00
abstract：:A procedure for studying 5-hydroxytryptamine synthesis by determining the rate of accumulation of 5-hydroxytryptophan after administering m-hydroxybenzylhydrazine, an inhibitor of aromatic-l-amino acid decarboxylase, and large doses of l-tryptophan was characterized. The utility of this method as an index of 5-hydroxy...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(86)90014-8
更新日期：1986-01-01 00:00:00
abstract：:Choline acetyltransferase activity was detected in extensively washed membranes prepared from rat and guinea pig synaptosomes. When these preparations were treated with the non-ionic detergent Triton X-114 and heated to 37 degrees C to cause phase separation, a significant percentage was found to associate with the de...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(88)90111-8
更新日期：1988-01-01 00:00:00
