Changes in serum histologic surrogate markers and procollagen III N-terminal peptide as independent predictors of HBeAg loss in patients with chronic hepatitis B during entecavir therapy.

abstract：OBJECTIVE:We compared leukocyte counts obtained by cytometric analysis and Fuchs-Rosenthal (FR) chamber counting in different proportions of lymphocytes (Lym%) suspensions and cerebrospinal fluid (CSF). DESIGN AND METHODS:UF-100 (UF) was evaluated. For preparation of cell suspensions, gradient density centrifugation m...

journal_title：Clinical biochemistry

authors： Yamanishi H,Imai N,Ohmine T,Nishiyama M,Suehisa E,Kanakura Y,Iwatani Y

更新日期：2006-08-01 00:00:00

abstract：OBJECTIVES:To evaluate whether caspases are involved in ethanol (EtOH)-induced apoptosis and if polyenylphosphatidylcholine (PPC) affects apoptosis, in vitro in Hep G2 cells. METHODS:Cells were treated with 100 mmol/L EtOH for 24 h and with 2 doses of 100 mmol/L EtOH (1/24 h) in the presence of absence of 20 mmol/L of...

journal_title：Clinical biochemistry

authors： Katz GG,Shear NH,Malkiewicz IM,Valentino K,Neuman MG

更新日期：2001-05-01 00:00:00

abstract：BACKGROUND:The aldosterone/renin ratio (ARR) is recommended to screen for primary aldosteronism (PA) in hypertension. We estimated fully automated chemiluminescence immunoassays (CLIA) for plasma aldosterone concentrations (PAC) and plasma direct renin concentrations (PRC) and investigated their reference intervals in ...

journal_title：Clinical biochemistry

authors： Deng L,Xiong Z,Li H,Lei X,Cheng L

更新日期：2018-06-01 00:00:00

abstract：OBJECTIVE:To review biochemical literature and determine the significance of non-covalent bonding in plasma. RESULTS:Many non-covalent bonds exist in plasma. Relatively strong self-associations are found for serum albumin, several gamma-globulins, apolipoproteins and fibrin(ogen). Here, fibrinogen and fibrin, consider...

journal_title：Clinical biochemistry

authors： London M

更新日期：1997-03-01 00:00:00

abstract：OBJECTIVE:Evaluation of serum beta-2-microglobulin (beta2M) in an automated analyzer. DESIGN AND METHODS:The DakoCytomation beta2M kit is an antibody based reagent intended for quantitative determination of beta2M in serum and plasma by rate nephelometry. RESULTS:The limit of blank is 0.16 mg/L. The method is linear ...

journal_title：Clinical biochemistry

authors： De BK,Jimenez E,De S

更新日期：2009-10-01 00:00:00

abstract：OBJECTIVE:Heat-mediated ELISA (HELISA) technique has been reported to shorten ELISA timings and has major clinical implications in diagnostic field [Bora U, Kannan K, Nahar P. Heat mediated enzyme linked immunosorbent assay. J Immunol Methods 2004; 293: 43-50]. Objective of this study is to find out whether anti-MBL au...

journal_title：Clinical biochemistry

authors： Sharma P,Gupta B,Farhat Basir S,Rani Das H,Nahar P

更新日期：2008-01-01 00:00:00

abstract：OBJECTIVES:Preeclampsia, the most common pregnancy-associated pathological syndrome, is accompanied by significant remodelling of the extracellular matrix and alteration in lipid composition of the umbilical cord artery (UCA). DESIGN AND METHODS:We evaluate the sphingolipid composition of UCA and its alteration in pre...

journal_title：Clinical biochemistry

authors： Romanowicz L,Bańkowski E

更新日期：2009-11-01 00:00:00

abstract：OBJECTIVE:Recently cortisol level in hair is supposed to be a good biomarker of chronic stress. We had developed a new method based on HPLC-FLU for identification of cortisol in human hair and measured cortisol distribution of each 1-cm segment along hair shaft. DESIGN AND METHODS:Hair sample was pulverized and incuba...

journal_title：Clinical biochemistry

authors： Gao W,Xie Q,Jin J,Qiao T,Wang H,Chen L,Deng H,Lu Z

更新日期：2010-05-01 00:00:00

abstract：OBJECTIVES:Measurement of serotonin concentrations in blood is considered to be important for the clinical studies of diseases involving vascular endothelial injury. We have developed a high-performance liquid chromatographic (HPLC) method for measurement of serotonin level in platelet-poor plasma (PPP). METHODS:Venou...

journal_title：Clinical biochemistry

authors： Hirowatari Y,Hara K,Kamihata H,Iwasaka T,Takahashi H

更新日期：2004-03-01 00:00:00

abstract：OBJECTIVES:Non-invasive prenatal aneuploidy testing (NIPT) by next-generation sequencing of circulating cell-free DNA in maternal plasma relies on chromosomal ratio (chrratio) measurements to detect aneuploid values that depart from euploid ratios. Diagnostic performances are known to depend on the fraction of fetal DN...

journal_title：Clinical biochemistry

authors： Blais J,Giroux S,Caron A,Clément V,Dionne-Laporte A,Jouan L,Gauthier J,MacLeod T,Moore R,Parker J,Swanson L,Zhao Y,Rouleau G,Karsan A,Langlois S,Rousseau F

更新日期：2018-09-01 00:00:00

abstract：OBJECTIVES:The aim of the study was to evaluate the concentration of interleukin 12 (IL-12), the activity of phospholipase A(2) (PLA(2)), and platelet-activating factor acetylhydrolase (PAF-AH) in type 1 diabetes (DM1) patients treated with intensive insulin therapy. DESIGN AND METHODS:Studied parameters were measured...

journal_title：Clinical biochemistry

authors： Wegner M,Araszkiewicz A,Pioruńska-Mikołajczak A,Zozulińska-Ziółkiewicz D,Wierusz-Wysocka B,Pioruńska-Stolzmann M

更新日期：2009-11-01 00:00:00

abstract：:Comparison of the turbidimetric immunoassay of haptoglobin with a reference method (the RID technique with appropriate correction for phenotype) clearly showed the turbidimetric assay to be phenotype-dependent. Correction factors for the three main phenotypes were calculated and reference values determined. The observ...

journal_title：Clinical biochemistry

authors： van Rijn HJ,van der Wilt W,Stroes JW,Schrijver J

更新日期：1987-08-01 00:00:00

abstract：:The measurement of serum 25-hydroxycalciferol (25-OH D) has proved to be a reliable index of the vitamin D nutritional status in man providing the renal 25-hydroxycalciferol-1 alpha-hydroxylase is functional. Since it is known that 25 OHD binds to alpha-globulins for its transport to the kidney, we have developed a co...

journal_title：Clinical biochemistry

authors： Delvin EE,Dussault M,Glorieux FH

更新日期：1980-06-01 00:00:00

abstract：:Although a multitude of hepatitis B markers are available to discriminate different states of hepatitis B virus (HBV) infection, there remains a need to detect the HBV DNA itself. The HBV DNA test is the only test that can quantify the number of complete virions. As shown in this review, the diagnostic value of testin...

journal_title：Clinical biochemistry

authors： Hess G,Reuschling M

更新日期：1993-08-01 00:00:00

abstract：OBJECTIVES:To monitor increases or decreases in cardiovascular disease (CVD)-related proteins that will be released from the deposits or plaque on the inner wall of blood vessels. DESIGN AND METHODS:Protein profiles of sera from healthy subjects and CVD patients were determined via 2-DE. Differentially expressed spots...

journal_title：Clinical biochemistry

authors： Kim HJ,Yoo HS,Kim PK,Kim MR,Lee HW,Kim CW

更新日期：2011-02-01 00:00:00

abstract：OBJECTIVES:Salivary analysis is a noninvasive alternative that may be more acceptable to patients, especially children. As such, it has the potential for incorporation into comprehensive, dynamic investigations of metabolic dysfunctions - a significant advancement over a single time point serum analysis. In this study,...

journal_title：Clinical biochemistry

authors： Chiu SK,Collier CP,Clark AF,Wynn-Edwards KE

更新日期：2003-05-01 00:00:00

abstract：OBJECTIVES:Sphingosine 1-phosphate (S1P) is carried in plasma by the HDL particles and albumin. It mediates several protective functions of HDL. Because of its barrier-enhancing effect, it has attracted attention in diseases associated with endothelial dysfunction. We examined the impact of circulating levels of S1P in...

journal_title：Clinical biochemistry

authors： Bekpinar S,Yenidunya G,Gurdol F,Unlucerci Y,Aycan-Ustyol E,Dinccag N

更新日期：2015-12-01 00:00:00

abstract：UNLABELLED:Experimental studies confirmed that reactive oxygen species increase endothelin-1 (ET-1) synthesis, and modulate ET-1 signaling pathway resulting in vasoconstriction and vascular remodeling. The aim of this study was to evaluate the relationship between plasma ET-1 concentration and antioxidant status in pat...

journal_title：Clinical biochemistry

authors： Skalska AB,Pietrzycka A,Stepniewski M

更新日期：2009-03-01 00:00:00

abstract：OBJECTIVES:To evaluate the evidence concerning gFOBT kits and to develop gFOBT standards to be implemented by the Ontario Colorectal Cancer (CRC) Screening Program (the "Program"). DESIGN AND METHODS:We evaluated 3 aspects of gFOBT kits: 1) performance factors; 2) usability factors; and 3) laboratory factors. We obtai...

journal_title：Clinical biochemistry

authors： Rabeneck L,Zwaal C,Goodman JH,Mai V,Zamkanei M

更新日期：2008-11-01 00:00:00

abstract：OBJECTIVES:: To test if circulating forms of the soluble urokinase-type plasminogen activator receptor (suPAR) are potential biomarkers of plaque vulnerability. DESIGN AND METHODS:: Plasma concentrations of suPAR(I-III), suPAR(II-III) and uPAR(I) were measured by time-resolved fluorescence immunoassays in Caucasian pa...

journal_title：Clinical biochemistry

authors： Olson FJ,Thurison T,Ryndel M,Høyer-Hansen G,Fagerberg B

更新日期：2010-01-01 00:00:00

abstract：UNLABELLED:Chronic liver diseases may cause inflammation and progressive scarring, over time leading to irreversible hepatic damage (cirrhosis). As a result, the need to assess and closely monitor individuals for risk factors of components of matrix deposition and degradation, as well as the severity of the fibrosis us...

journal_title：Clinical biochemistry

authors： Neuman MG,Cohen LB,Nanau RM

更新日期：2016-02-01 00:00:00

abstract：BACKGROUND:Niemann-Pick type C (NP-C) is a rare progressive neurodegenerative lipid storage disorder with heterogeneous clinical presentation and challenging diagnostic procedures. Recently oxysterols have been reported to be specific biomarkers for NP-C but knowledge on the intra-individual variation and on reference ...

journal_title：Clinical biochemistry

authors： Klinke G,Rohrbach M,Giugliani R,Burda P,Baumgartner MR,Tran C,Gautschi M,Mathis D,Hersberger M

更新日期：2015-06-01 00:00:00

abstract：OBJECTIVE:Mycophenolic acid requires routine therapeutic drug monitoring. We evaluated the suitability of a new PETINIA (particle enhanced turbidimetric inhibition immunoassay) assay on the Dimension EXL analyzer for monitoring of mycophenolic acid by comparing values obtained by this assay with a HPLC-UV method. DESI...

journal_title：Clinical biochemistry

authors： Dasgupta A,Tso G,Chow L

更新日期：2013-05-01 00:00:00

abstract：BACKGROUND:The effectiveness of the URIT-2900 Hematology Analyzer for screening of hemoglobinopathies commonly found in Southeast Asian populations was examined. METHODS:Appropriate cut-off values of MCV and MCH for screening of α(0) and β thalassemias were derived from the receiver operator characteristic curve condu...

journal_title：Clinical biochemistry

authors： Karnpean R,Pansuwan A,Fucharoen G,Fucharoen S

更新日期：2011-07-01 00:00:00

abstract：BACKGROUND:We evaluated the diagnostic efficacy of urinary angiogenin (ANG) and cytokeratin 20 (CK-20) mRNA in comparison with voided urine cytology in the detection of bladder cancer patients. OBJECTIVES AND METHODS:A total of 97 Egyptian patients provided a single voided urine sample for ANG, CK-20 and cytology befo...

journal_title：Clinical biochemistry

authors： Eissa S,Kenawy G,Swellam M,El-Fadle AA,Abd El-Aal AA,El-Ahmady O

更新日期：2004-09-01 00:00:00

abstract：OBJECTIVES:The aims of the study were to test the susceptibility of THP-1 macrophages to develop oxidative stress and to deploy antioxidant defense mechanisms that insure the balance between the pro- and antioxidant molecules. DESIGN AND METHODS:Differentiated THP-1 were incubated in the presence or absence of iron-as...

journal_title：Clinical biochemistry

authors： Marcil V,Lavoie JC,Emonnot L,Seidman E,Levy E

更新日期：2011-07-01 00:00:00

abstract：OBJECTIVES:Living donor kidney transplantation is regarded as beneficial to allograft recipients and not particularly detrimental to the donors. Recently we have documented a reduced glomerular filtration rate (GFR) in living kidney donors (LKDs). Considering kidneys as essential for l-arginine (Arg) metabolism, in the...

journal_title：Clinical biochemistry

authors： Zunić G,Tomić A,Spasić S

更新日期：2013-10-01 00:00:00

abstract：OBJECTIVE:Third generation oral contraceptives (OC) may increase the risk of cardiovascular events in healthy young women. DESIGN AND METHODS:We assessed the effect of second and third generation OC on CRP, lipids and apolipoproteins in 128 women. RESULTS:CRP was significantly higher in third generation contraceptive...

journal_title：Clinical biochemistry

authors： Krintus M,Sypniewska G,Kuligowska-Prusinska M

更新日期：2010-04-01 00:00:00

abstract：OBJECTIVE:To investigate the feasibility of detecting circulating cancer cells in peripheral blood from gastric cancer patients using Piwi-interacting RNAs (piRNAs) as markers. DESIGN AND METHODS:Real-time quantitative reverse transcription-polymerase chain reaction was used to analyze piR-651 and piR-823 levels in th...

journal_title：Clinical biochemistry

authors： Cui L,Lou Y,Zhang X,Zhou H,Deng H,Song H,Yu X,Xiao B,Wang W,Guo J

更新日期：2011-09-01 00:00:00

abstract：OBJECTIVES:Newly presented solid-phase extraction methods (Solid Phase Disc Extraction, SPEC-Plus, Multi-Modal, and Solid Phase Micro Extraction, SPME have been checked with respect to their applicability to clinical toxicological analysis. In comparison with conventional liquid/liquid-extraction and a common mixed-pha...

journal_title：Clinical biochemistry

authors： Degel F

更新日期：1996-12-01 00:00:00