Abstract：
:Specific binding of [3H](+-)-3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP), a highly selective antagonist for N-methyl-D-aspartic acid (NMDA) receptors, was examined in brain synaptic membranes treated with Triton X-100 by using a filtration assay method. Elevation of incubation temperature from 2 to 30 degrees C markedly diminished the binding. The binding reached a plateau within 5 min after the initiation of incubation at 2 degrees C, while the time required to attain an equilibrium was 1 min at 30 degrees C. The binding at 2 degrees C was rapidly dissociated by the addition of an excess of unlabeled CPP, NMDA and L-glutamic acid (L-Glu). The binding was also saturable with increasing concentrations of the ligand and displaced by various amino acids structurally related to L-Glu in a stereospecific manner. Competitive but not noncompetitive antagonists for the NMDA receptors invariably inhibited the binding. However, the binding was not prominently affected by agonists for the other subclasses of the brain excitatory amino acid receptors. Both reduced and oxidized forms of glutathione significantly displaced the binding. Scatchard analysis revealed that Triton treatment increased the affinity and density of binding sites which consisted of a single component. Among some endogenous tryptophan metabolites, kynurenic, anthranilic and quinolinic acids inhibited the binding. These results suggest that a filtration assay method is also useful to detect the binding of NMDA receptors in the brain.
journal_name
Brain Resjournal_title
Brain researchauthors
Ogita K,Yoneda Ydoi
10.1016/0006-8993(90)90575-vsubject
Has Abstractpub_date
1990-05-07 00:00:00pages
51-6issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(90)90575-Vjournal_volume
515pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Drugs thought to inhibit the actions of protein kinase C (PKC) and cAMP dependent protein kinase (A-kinase) were infused intrathecally into the subarachnoid space of the lumbar region of the spinal cord, and the effects on acoustic startle were measured. Previous work has shown that intrathecal infusion of drugs thoug...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90864-8
更新日期：1990-08-20 00:00:00
abstract：:Electrical synaptic transmission via gap junctions has become an accepted feature of neuronal communication in the mammalian brain, and occurs often between dendrites of interneurons in major brain structures, including the hippocampus. Electrical and dye-coupling has also been reported to occur between pyramidal cell...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.05.064
更新日期：2012-12-03 00:00:00
abstract：:Understanding the structural and functional development of neurons in networks has a high impact to estimate the potentials for restorative therapies. Neurons derived from the human NT2 cell line (hNT) formed networks with a clustered neuritic architecture in vitro, whereas primary dissociated embryonic rat cortical n...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.05.076
更新日期：2004-08-20 00:00:00
abstract：:It was previously reported that chronic food restriction and maintenance of rats at 75-80% of initial body weight enhanced the reward-potentiating effect of D-amphetamine in the lateral hypothalamic self-stimulation (LHSS) paradigm. Moreover, the enhancement reversed in parallel with body weight recovery when ad libit...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.03.002
更新日期：2006-05-04 00:00:00
abstract：:To identify the subtype of dopamine receptors critically involved in the rewarding effect of brain stimulation, four dopamine antagonists were intracranially injected in 25 rats. The importance of dopamine D1 receptors had been demonstrated previously by using SCH 23390, a highly selective D1 antagonist. Rats were imp...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00304-1
更新日期：1997-06-20 00:00:00
abstract：:Iontophoresis of baclofen produced hyperpolarization and a decrease in input resistance in 32 neurons and no effect in 24 neurons of the ventral respiratory group in cats. Iontophoresed phaclofen antagonized the effect of baclofen, but had negligible effects on periodic fluctuations in membrane potential and spike act...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90832-8
更新日期：1993-09-17 00:00:00
abstract：:Recent studies suggest that selective block of extrasynaptic N-methyl-d-aspartate (NMDA) receptors might protect against neurodegeneration. We recorded whole-cell currents with patch pipettes to characterize the ability of memantine, a low-affinity NMDA channel blocker, to block synaptic and extrasynaptic NMDA recepto...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2015.01.041
更新日期：2015-04-07 00:00:00
abstract：:The responses of spinocervical neurons to sinusoidal hair displacements were studied during and in the absence of radiant heating of parts of the hindpaw to noxious levels (45-65 degrees C). Noxious heat usually increased background discharge and lowered the signal-to-noise ratio at low frequencies of hair displacemen...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90949-5
更新日期：1981-10-05 00:00:00
abstract：:Production of inflammatory cytokines, including tumor necrosis factor (TNF), interleukin-1 (IL-1) and interleukin-6 (IL-6), in the brain is increased in various diseases. To investigate the relationships between the effect of overproduction of IL-6 in the brain on central and peripheral production of TNF, IL-1 beta an...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(96)00865-7
更新日期：1996-11-18 00:00:00
abstract：:The development from primary to augmenting responses of somatosensory (SI) cortical area to low-frequency stimulation of the ventrobasal (VB) thalamus or white matter (WM) beneath SI in VB-lesioned preparations was studied by field potential analysis and extracellular unit recording. Compared to the major postsynaptic...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90719-8
更新日期：1981-01-26 00:00:00
abstract：:The carrier-mediated uptake of anionic and cationic amino acids by the basolateral face (blood side) of the isolated perfused sheep choroid plexus was demonstrated using the paired-tracer dilution technique. The uptake of these two classes of amino acid was higher than at the blood-brain barrier with no cross-competit...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90731-n
更新日期：1992-05-29 00:00:00
abstract：:Androgens are involved in a variety of centrally mediated functions after binding to their intracellular receptors. In the present report, we have employed the androgen receptor antibody, PG-21, and indirect immunohistochemistry to examine the distribution of cells containing androgen receptor-like immunoreactivity (A...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00983-3
更新日期：1995-12-12 00:00:00
abstract：:We have found that daily subcutaneous injection with a maximum tolerated dose (MTD) of the mGluR2/3 agonist LY379268 (20mg/kg) beginning at 4 weeks dramatically improves the phenotype in R6/2 mice. For example, we observed normalization of motor function in distance traveled, speed, the infrequency of pauses, and the ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.07.026
更新日期：2012-09-14 00:00:00
abstract：:Modulation of gamma-aminobutyric acid (GABA)-mediated inhibition, and glutamate-mediated excitation by highly selective mu and delta opioid agonists was studied using intracellular recordings of CA1 pyramidal neuron synaptic responses in superfused hippocampal slices. Equimolar concentrations of the selective mu agoni...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)91312-3
更新日期：1992-10-16 00:00:00
abstract：:In the developing vertebrate retina, precise coordination of retinal progenitor cell proliferation and cell-cycle exit is essential for the formation of a functionally mature retina. Unregulated or disrupted cell proliferation may lead to dysplasia, retinal degeneration or retinoblastoma. Both cell-intrinsic and -extr...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2007.04.076
更新日期：2008-02-04 00:00:00
abstract：:A selective 5-HT reuptake inhibitor, fluvoxamine (10 and 30 mg/kg, i.p.) suppressed long-term potentiation (LTP) in the hippocampal CA1 field of anesthetized rats. Fluvoxamine (30 mg/kg, i.p.)-induced suppression of LTP was completely reversed by the 5-HT(1A) receptor antagonist NAN-190 (0.5 mg/kg, i.p), but not by th...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03756-3
更新日期：2003-01-03 00:00:00
abstract：:Adenosine, agmatine and kynurenic acid are endogenous ligands acting on different (e.g. adenosine, NMDA, alpha(2)-adrenergic and imidazoline) receptors with a potential role in nociception at the spinal level. Their antinociceptive effects have already been investigated as monotherapy, but only a few studies have repo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.09.039
更新日期：2004-12-10 00:00:00
abstract：:Previously, we have shown that intracisternal (i.c.) administration of beta-endorphin suppresses brain and liver DNA synthesis in rat pups. This finding is consistent with the view that endogenous CNS beta-endorphin plays an important role in controlling postnatal growth. Recent evidence suggests that brain CCK8, the ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)91175-4
更新日期：1994-10-24 00:00:00
abstract：:Somatostatin-like immunoreactivity (Som-LI) and glycine high-affinity uptake have been characterized in the Rana pipiens retina. These labels are found in both the outer and inner plexiform layers (OPL and IPL), suggesting that interplexiform cells (IPCs) contain both Som and glycine in this retina. In double-label ex...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91215-8
更新日期：1989-08-21 00:00:00
abstract：:Retrograde axonal transport method of the fluorescent tracer FluoroGold (FG) was combined with immunocytochemistry to investigate the occurrence of nitric oxide synthase (NOS), somatostatin (SOM), neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP) in both intrinsic and cortically projecting neurons of the ra...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02408-8
更新日期：2001-06-29 00:00:00
abstract：:Two pathways that have been shown to mediate cerebral ischemic damage are the MEK/ERK cascade and the pro-apoptotic deltaPKC pathway. We investigated the relationship between these pathways in a rat model of focal ischemia by observing and modifying the activation state of each pathway. The ERK1/2 inhibitor, U0126, in...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.11.051
更新日期：2009-01-28 00:00:00
abstract：:The immunosuppressive macrolide FK-506 has been shown to protect neurons in culture against glutamate excitotoxicity. This effect was attributed to the binding of immunosuppressants to calcineurin-inhibiting immunophilins. We now report that also the non-immunosuppressive macrolide antibiotics protect neurons in cultu...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90098-8
更新日期：1993-10-08 00:00:00
abstract：:To determine whether post-traumatic seizure severity would be affected by the interval between seizures and head injury, we measured seizures after various times with or without fluid percussion brain injury (2atm fluid percussion injury; FPI). To determine efficacy of anti-seizure medication, we also determined if le...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.04.013
更新日期：2016-07-01 00:00:00
abstract：:A microdialysis technique for the in vivo assessment of the monoaminergic and GABAergic levels in the ventromedial (VMN) and paraventricular nuclei (PVN) of the hypothalamus was used in order to examine the activities of neurons that project to the hypothalamic regions and are implicated in the regulation of ingestive...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01796-5
更新日期：1999-09-18 00:00:00
abstract：:Pouch young opossums aged from 5 to 34 days underwent unilateral eye enucleation and the projection from the remaining eye to the superior colliculus (SC) was explored at maturity by anatomical and physiological methods. The results were compared to data from normal or adult enucleated animals. Analysis of the experim...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(86)90236-1
更新日期：1986-06-01 00:00:00
abstract：:Freeze-fracture examination of nodes of Ranvier from adult rat optic nerve demonstrates the presence of astrocytic processes at the majority of nodes of Ranvier. Astrocytic processes often run along the entire length of the nodal gap, although they do not necessarily encircle the entire nodal circumference. The E- and...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90919-3
更新日期：1984-08-06 00:00:00
abstract：:The effects of pentylenetetrazol (PTZ) following induction of long-term potentiation (LTP) on population spikes in CA1 of hippocampal slices were investigated. Population spikes were evoked by activation of Schaffer collaterals with a range of stimulation intensities. LTP was induced using θ-pattern primed burst...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02672-x
更新日期：2000-09-22 00:00:00
abstract：:Rats were trained on a radial maze and then given electrolytic lesions of the MS followed by a single intraseptal injection of 5 micrograms of NGF. Three days later they were re-tested on the maze. They were also post-operatively tested for hyperemotionality. MS lesions severely impaired performance on the radial maze...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00242-i
更新日期：1995-05-08 00:00:00
abstract：:PGP 9.5 is a new cytoplasmic neuron-specific protein structurally and immunologically distinct from neuron-specific enolase. A specific antiserum has now shown that this protein is widely distributed in vertebrate brains and is also present in cells of the human diffuse neuroendocrine system. PGP 9.5 appears to be old...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90241-x
更新日期：1983-11-14 00:00:00
abstract：:After transplantation of autologous sciatic nerve segments into the retina of adult hamsters for 1-2 months, retrograde labelling with horseradish peroxidase demonstrated a population of ganglion cells situated peripheral to the graft. If an additional lesion was placed between the insertion of the graft and the optic...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91203-5
更新日期：1986-07-02 00:00:00
