2,4-Dichlorophenoxyacetic Acid (2,4-D)- and 2,4,5-Trichlorophenoxyacetic Acid (2,4,5-T)-Degrading Bacterial Community in Soil-Water Suspension during the Enrichment Process.

Abstract:

:Changes in the bacterial community in soil-water suspensions during the enrichment period of 2,4-D and 2,4,5-T degraders were examined using denaturing gradient gel electrophoresis (DGGE) analysis of the 16S rRNA gene. The nucleotide sequences of almost all major bands at the degradation of 2,4-D and 2,4,5-T corresponded to those of 2,4-D- and 2,4,5-T-degrading isolates, and successions of diverse 2,4-D- and 2,4,5-T-degrading bacterial communities were demonstrated in the DGGE profile. These results suggested that 2,4-D- and 2,4,5-T-degrading isolates were responsible for the degradation of 2,4-D and 2,4,5-T in soil-water suspensions; however, one major band of a non-degrader was found in some cases. Therefore, to elucidate the relationship between the structures and functions of bacterial communities, the importance of a combined approach using both culture-dependent and -independent methods was demonstrated.

journal_name

Microbes Environ

authors

Huong NL,Itoh K,Suyama K

doi

10.1264/jsme2.23.142

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

142-8

issue

2

eissn

1342-6311

issn

1347-4405

pii

JST.JSTAGE/jsme2/23.142

journal_volume

23

pub_type

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