Abstract:
:Changes in the bacterial community in soil-water suspensions during the enrichment period of 2,4-D and 2,4,5-T degraders were examined using denaturing gradient gel electrophoresis (DGGE) analysis of the 16S rRNA gene. The nucleotide sequences of almost all major bands at the degradation of 2,4-D and 2,4,5-T corresponded to those of 2,4-D- and 2,4,5-T-degrading isolates, and successions of diverse 2,4-D- and 2,4,5-T-degrading bacterial communities were demonstrated in the DGGE profile. These results suggested that 2,4-D- and 2,4,5-T-degrading isolates were responsible for the degradation of 2,4-D and 2,4,5-T in soil-water suspensions; however, one major band of a non-degrader was found in some cases. Therefore, to elucidate the relationship between the structures and functions of bacterial communities, the importance of a combined approach using both culture-dependent and -independent methods was demonstrated.
journal_name
Microbes Environjournal_title
Microbes and environmentsauthors
Huong NL,Itoh K,Suyama Kdoi
10.1264/jsme2.23.142subject
Has Abstractpub_date
2008-01-01 00:00:00pages
142-8issue
2eissn
1342-6311issn
1347-4405pii
JST.JSTAGE/jsme2/23.142journal_volume
23pub_type
杂志文章abstract::The effects of a nitrogen-fixing tree, black locust (Robinia pseudoacacia), on the distribution of bacterial species were examined in a Japanese black pine (Pinus thunbergii) and black locust-dominated area. DNA was extracted from the soil at depths of 0-5 and 5-10 cm, collected at the border between a Japanese black ...
journal_title:Microbes and environments
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doi:10.1264/jsme2.me08560
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journal_title:Microbes and environments
pub_type: 杂志文章,评审
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更新日期:2020-01-01 00:00:00
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journal_title:Microbes and environments
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doi:10.1264/jsme2.ME17162
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journal_title:Microbes and environments
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doi:10.1264/jsme2.me08526
更新日期:2008-01-01 00:00:00
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journal_title:Microbes and environments
pub_type: 杂志文章
doi:10.1264/jsme2.ME16107
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journal_title:Microbes and environments
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doi:10.1264/jsme2.me10119
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pub_type: 杂志文章
doi:10.1264/jsme2.me11331
更新日期:2012-01-01 00:00:00
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journal_title:Microbes and environments
pub_type: 杂志文章
doi:10.1264/jsme2.me08540
更新日期:2009-01-01 00:00:00
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journal_title:Microbes and environments
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journal_title:Microbes and environments
pub_type: 杂志文章,评审
doi:10.1264/jsme2.me13159
更新日期:2014-01-01 00:00:00
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journal_title:Microbes and environments
pub_type: 杂志文章
doi:10.1264/jsme2.23.209
更新日期:2008-01-01 00:00:00
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journal_title:Microbes and environments
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journal_title:Microbes and environments
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journal_title:Microbes and environments
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journal_title:Microbes and environments
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更新日期:2009-01-01 00:00:00
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journal_title:Microbes and environments
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journal_title:Microbes and environments
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