Development of a hierarchical oligonucleotide primer extension assay for the qualitative and quantitative analysis of Cylindrospermopsis raciborskii subspecies in freshwater.

abstract：:Arsenic (As) is a widely distributed toxic element in the environment and microorganisms have developed resistance mechanisms in order to tolerate it. The cellular response of the chemoorganotrophic arsenite (As[III])-oxidizing α-Proteobacteria, Sinorhizobium sp. strain A2, to arsenic was examined in the present study...

journal_title：Microbes and environments

authors： Fukushima K,Huang H,Hamamura N

更新日期：2015-01-01 00:00:00

abstract：:The emergence of drug-resistant bacteria is a severe problem in aquaculture. The ability of drug resistance genes to transfer from a bacterial cell to another is thought to be responsible for the wide dissemination of these genes in the aquaculture environment; however, little is known about the gene transfer mechanis...

journal_title：Microbes and environments

authors： Nonaka L,Maruyama F,Miyamoto M,Miyakoshi M,Kurokawa K,Masuda M

更新日期：2012-01-01 00:00:00

abstract：:The present study aimed to reveal the eukaryotic diets of two economically important marine sediment-inhabiting worms, Sipunculus nudus (peanut worm) and Urechis unicinctus (spoon worm), using clone libraries and phylogenetic analyses of 18S rRNA genes. Fungal rDNA was also targeted and analyzed to reveal mycobiomes. ...

journal_title：Microbes and environments

authors： Wang Y,Shi T,Huang G,Gong J

更新日期：2018-09-29 00:00:00

abstract：:We established artificial consortia of bacteria inoculated from soil and a green alga, Chlorella vulgaris NIES-227. The bacteria and the alga were mixed in a tube or partitioned in a dialysing culture vessel, and the bacterial composition was examined after cultivation. The community of bacteria formed in the consorti...

journal_title：Microbes and environments

authors： Ueda H,Otsuka S,Senoo K

更新日期：2010-01-01 00:00:00

abstract：:Atrazine is a triazine herbicide that is widely used to control broadleaf weeds. Its widespread use over the last 50 years has led to the potential contamination of soils, groundwater, rivers, and lakes. Its main route of complete degradation is via biological means, which is carried out by soil microbiota using a 6-s...

journal_title：Microbes and environments

authors： Fernandes AFT,Wang P,Staley C,Aparecida Silva Moretto J,Miguel Altarugio L,Chagas Campanharo S,Guedes Stehling E,Jay Sadowsky M

更新日期：2020-01-01 00:00:00

abstract：:The simultaneous extraction of plant organelle (mitochondria and plastid) genes during the DNA extraction step is a major limitation in investigating the community structures of bacteria associated with plants because organelle SSU rRNA genes are easily amplified by PCR using primer sets that are specific to bacteria....

journal_title：Microbes and environments

authors： Ikenaga M,Sakai M

更新日期：2014-09-17 00:00:00

abstract：:Denitrifiers can produce and consume nitrous oxide (N(2)O). While little N(2)O is emitted from rice paddy soil, the same soil produces N(2)O when the land is drained and used for upland crop cultivation. In this study, we collected soils from two types of fields each at three locations in Japan; one type of field had ...

journal_title：Microbes and environments

authors： Tago K,Ishii S,Nishizawa T,Otsuka S,Senoo K

更新日期：2011-01-01 00:00:00

abstract：:Diarrhea is often associated with marked alterations in the intestinal microbiota, termed dysbiosis; however, limited information is currently available on the intestinal microbiota in captive golden snub-nosed monkeys (Rhinopithecus roxellana) with diarrhea. We herein characterized the fecal microbiota in diarrhea an...

journal_title：Microbes and environments

authors： Zhu H,Zeng D,Wang Q,Wang N,Zeng B,Niu L,Ni X

更新日期：2018-09-29 00:00:00

abstract：:The mining industry generates huge amounts of wastewater, containing toxic heavy metals. Treatment to remove heavy metals is necessary and recent work has been focused on finding more environmentally friendly materials for removing heavy metals from wastewater. Biosorption can be an effective process for heavy metal r...

journal_title：Microbes and environments

authors： Mohamad OA,Hao X,Xie P,Hatab S,Lin Y,Wei G

更新日期：2012-01-01 00:00:00

abstract：:A cellular approach combining Direct Viable Counting and Fluorescent In Situ Hybridization using a one-step multiple-probe technique and Solid Phase Cytometry (DVC-FISH-SPC) was developed to monitor total viable vibrios and cover the detection of a large diversity of vibrios. FISH combined three probes in the same ass...

journal_title：Microbes and environments

authors： Girard L,Peuchet S,Servais P,Henry A,Charni-Ben-Tabassi N,Baudart J

更新日期：2017-09-27 00:00:00

abstract：:Members of the Marseilleviridae family are large DNA viruses with icosahedral particle structures that infect Acanthamoeba cells. The first Marseillevirus to be discovered was isolated in 2009. Since then, several other members of the Marseilleviridae family have been reported, including Lausannevirus, Senegalvirus, C...

journal_title：Microbes and environments

authors： Takemura M

更新日期：2016-12-23 00:00:00

abstract：:Potato peels consist of a tissue called phellem, which is formed by suberized cell layers. The degradation of suberin, a lipidic and recalcitrant polymer, is an ecological process attributed to soil fungal populations; however, previous studies have suggested that Streptomyces scabiei, the causal agent of potato commo...

journal_title：Microbes and environments

authors： Beaulieu C,Sidibé A,Jabloune R,Simao-Beaunoir AM,Lerat S,Monga E,Bernards MA

更新日期：2016-12-23 00:00:00

abstract：:Effects of different land uses on densities of Escherichia coli, enterococci, and Clostridium perfringens in the water and soil of a tropical watershed were investigated. Densities of fecal indicator bacteria (FIBs) in the watershed exhibited a clear land-use dependency in the stream water. Significantly higher concen...

journal_title：Microbes and environments

authors： Goto DK,Yan T

更新日期：2011-01-01 00:00:00

abstract：:The screening of pollutant degraders by relying solely on cultivation techniques such as liquid enrichment often fails to isolate the actual degraders in the environment. Community analyses by PCR-denaturing gradient gel electrophoresis (DGGE) were performed to isolate bacteria that can degrade 3-chlorobenzoate (3CB) ...

journal_title：Microbes and environments

authors： Morimoto S,Ogawa N,Hasebe A,Fujii T

更新日期：2008-01-01 00:00:00

abstract：:The Gram-negative marine propylene-assimilating bacterium, strain PE-TB08W, was isolated from surface seawater. A structural gene analysis using the 16S rRNA gene showed 96, 94, and 95% similarities to Halioglobus species, Haliea sp. ETY-M, and Haliea sp. ETY-NAG, respectively. A phylogenetic tree analysis showed that...

journal_title：Microbes and environments

authors： Suzuki T,Yazawa T,Morishita N,Maruyama A,Fuse H

更新日期：2019-03-30 00:00:00

abstract：:We investigated biofilm formation on biodegradable plastics in freshwater samples. Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBH) was covered by a biofilm after an incubation in freshwater samples. A next generation sequencing analysis of the bacterial communities of biofilms that formed on PHBH films revealed t...

journal_title：Microbes and environments

authors： Morohoshi T,Oi T,Aiso H,Suzuki T,Okura T,Sato S

更新日期：2018-09-29 00:00:00

abstract：:We investigated the effects of the water status (flooded or non-flooded) and presence of the nosZ gene in bradyrhizobia on the bradyrhizobial community structure in a factorial experiment that examined three temperature levels (20°C, 25°C, and 30°C) and two soil types (andosol and gray lowland soil) using microcosm in...

journal_title：Microbes and environments

authors： Saeki Y,Nakamura M,Mason MLT,Yano T,Shiro S,Sameshima-Saito R,Itakura M,Minamisawa K,Yamamoto A

更新日期：2017-06-24 00:00:00

abstract：:Soybean plants host endosymbiotic dinitrogen (N2)-fixing bacteria from the genus Bradyrhizobium. Under oxygen-limiting conditions, Bradyrhizobium diazoefficiens and Bradyrhizobium japonicum perform denitrification by sequentially reducing nitrate (NO3-) to nitrous oxide (N2O) or N2. The anaerobic reduction of NO3- to ...

journal_title：Microbes and environments

authors： Siqueira AF,Sugawara M,Arashida H,Minamisawa K,Sánchez C

更新日期：2020-01-01 00:00:00

abstract：:The role of microbes in the early development of ecosystems on new volcanic materials seems to be crucial to primary plant succession but is not well characterized. Here we analyzed the bacterial community colonizing 22-year-old volcanic deposits of the Miyake-jima Island (Japan) using culture-based and 16S rRNA gene ...

journal_title：Microbes and environments

authors： Lu H,Fujimura R,Sato Y,Nanba K,Kamijo T,Ohta H

更新日期：2008-01-01 00:00:00

abstract：:Microscopic and molecular analyses showed the presence of endobacteria inside the mycelia of four out of twelve nitrous oxide (N(2)O)-producing fungal isolates identified as Mortierella elongata. The 16S rRNA gene was successfully amplified with DNA extracted directly from the endobacterium-containing fungal strains a...

journal_title：Microbes and environments

authors： Sato Y,Narisawa K,Tsuruta K,Umezu M,Nishizawa T,Tanaka K,Yamaguchi K,Komatsuzaki M,Ohta H

更新日期：2010-01-01 00:00:00

abstract：:Termites depend nutritionally on their gut microbes, and protistan, bacterial, and archaeal gut communities have been extensively studied. However, limited information is available on viruses in the termite gut. We herein report the complete genome sequence (99,517 bp) of a phage obtained during a genome analysis of "...

journal_title：Microbes and environments

authors： Pramono AK,Kuwahara H,Itoh T,Toyoda A,Yamada A,Hongoh Y

更新日期：2017-06-24 00:00:00

abstract：:HcRNAV is a small icosahedral virus that infects the shellfish-killing marine dinoflagellate Heterocapsa circularisquama, which harbors a dicistronic linear single-stranded RNA (ssRNA) genome ca. 4.4 kb in length. Its major capsid protein (MCP) gene sequence is not expressed by various strains of Escherichia coli, pos...

journal_title：Microbes and environments

authors： Wada K,Kimura K,Hasegawa A,Fukuyama K,Nagasaki K

更新日期：2012-01-01 00:00:00

abstract：:Branched nonylphenol (BNP), a degradation product of nonylphenol polyethoxylates, exerts estrogenic effects on various organisms. The genes underlying BNP degradation by Sphingobium amiense DSM 16289T were analyzed by complete genome sequencing and compared with those of the versatile BNP-degrading Sphingobium cloacae...

journal_title：Microbes and environments

authors： Ootsuka M,Nishizawa T,Hasegawa M,Kurusu Y,Ohta H

更新日期：2018-12-28 00:00:00

abstract：:Giant viruses of 'Megaviridae' have the ability to widely disperse around the globe. We herein examined 'Megaviridae' communities in four distinct aquatic environments (coastal and offshore seawater, brackish water, and hot spring freshwater), which are distantly located from each other (between 74 and 1,765 km), usin...

journal_title：Microbes and environments

authors： Li Y,Endo H,Gotoh Y,Watai H,Ogawa N,Blanc-Mathieu R,Yoshida T,Ogata H

更新日期：2019-09-25 00:00:00

abstract：:The effects of a nitrogen-fixing tree, black locust (Robinia pseudoacacia), on the distribution of bacterial species were examined in a Japanese black pine (Pinus thunbergii) and black locust-dominated area. DNA was extracted from the soil at depths of 0-5 and 5-10 cm, collected at the border between a Japanese black ...

journal_title：Microbes and environments

authors： Taniguchi T,Kataoka R,Tamai S,Yamanaka N,Futai K

更新日期：2009-01-01 00:00:00

abstract：:The aim of the study was to survey cultivable heterotrophic bacteria from sea ice samples collected from Peter the Great Bay of the Sea of Japan, and to test the isolates for antagonistic activity. A total of 195 strains were isolated and investigated by a phenotypic approach. The 16S rRNA gene sequence analysis of th...

journal_title：Microbes and environments

authors： Romanenko LA,Tanaka N,Uchino M,Kalinovskaya NI,Mikhailov VV

更新日期：2008-01-01 00:00:00

abstract：:Members of the Legionella genus find suitable conditions for their growth and survival in nuclear power plant cooling circuits. To limit the proliferation of Legionella pathogenic bacteria in nuclear power plant cooling circuits, and ensure that levels remain below regulatory thresholds, monochloramine treatment can b...

journal_title：Microbes and environments

authors： Jakubek D,Guillaume C,Binet M,Leblon G,DuBow M,Le Brun M

更新日期：2013-01-01 00:00:00

abstract：:The splicing of a bacterial group II subclass B intron B.me.I1 from Bacillus megaterium chromosomes was investigated. RT-PCR and nucleic acid hybridization methods were used to understand the role of the intron-encoded protein (IEP) in the splicing of B.me.I1. An in vivo assay showed that the splicing occurred in the ...

journal_title：Microbes and environments

authors： Chien MF,Tosa S,Huang CC,Endo G

更新日期：2009-01-01 00:00:00

abstract：:The nifV gene encodes homocitrate synthase, the enzyme that catalyzes the formation of homocitrate, which is essential for arranging the FeMo-cofactor in the catalytic center of nitrogenase. Some host plants, such as Lotus japonicus, supply homocitrate to their symbionts, in this case, Mesorhizobium loti, which lacks ...

journal_title：Microbes and environments

authors： Hashimoto S,Wongdee J,Songwattana P,Greetatorn T,Goto K,Tittabutr P,Boonkerd N,Teaumroong N,Uchiumi T

更新日期：2019-12-27 00:00:00

abstract：:This study aimed to characterize soil fungal communities in upland rice fields managed with tillage/non-tillage and winter cover-cropping (hairy vetch and cereal rye) practices, using PCR-based molecular methods. The study plots were maintained as upland fields for 5 years and the soils sampled in the second and fifth...

journal_title：Microbes and environments

authors： Nishizawa T,Zhaorigetu,Komatsuzaki M,Sato Y,Kaneko N,Ohta H

更新日期：2010-01-01 00:00:00