Abstract:
AIM:Increased levels of small dense low-density lipoproteins (sd-LDL) have been reported more atherogenic compared to total low-density lipoprotein (LDL); however, no definitive experiments using macrophages have examined this concept in vitro. METHOD AND RESULT:In this study, we isolated fractions of total LDL (density 1.019-1.063 g/ml) and sd-LDL (density 1.044-1.063 g/ml) from the plasma of subjects with modest hypertriglycidemia. Oxidizabilty as assessed by copper-induced generation (1.6 µmol/L CuSO(4),12 h) of thiobarbituric acid reactive substances (TBARS) was significantly greater (7-fold higher, p < 0.01) for sd-LDL (4.3 ± 1.1 nmol/mg) than for total LDL (0.6 ± 0.2 nmol/mg) at the same cholesterol concentrations. Moreover, oxidized sd-LDL induced more lipid staining in macrophages than oxidized total LDL. When non-oxidized sd-LDL were incubated with THP1 macrophages, there was much greater lipid accumulation as assessed by oil red O staining, and more than a 2-fold increase (p < 0.05) in intracellular triglyceride content as compared to non-oxidized total LDL. Furthermore, non-oxidized sd-LDL in contrast to non-oxidized total LDL enhanced macrophage lectin-like oxidized LDL receptor-1 (LOX-1) protein expression and significantly LOX-1 mRNA levels (+158%, p < 0.05), with no effect on scavenger receptor A or CD36 gene expression. These effects of non-oxidized sd-LDL on LOX-1 gene expression were suppressed when Toll-like receptor 4 was inactivated either by RNAi or antibody. CONCLUSION:Our data indicate for the first time that sd-LDL is much more effective in promoting macrophage triglyceride accumulation and LOX-1 gene expression than total LDL.
journal_name
J Atheroscler Thrombjournal_title
Journal of atherosclerosis and thrombosisauthors
Tani M,Kawakami A,Mizuno Y,Imase R,Ito Y,Kondo K,Ishii H,Yoshida Mdoi
10.5551/jat.7161subject
Has Abstractpub_date
2011-01-01 00:00:00pages
698-704issue
8eissn
1340-3478issn
1880-3873pii
JST.JSTAGE/jat/7161journal_volume
18pub_type
杂志文章abstract::Cell degeneration and collagenosis are the main features in atherosclerotic plaque. We examined the effects of human low density lipoprotein (LDL) on cultured rabbit aortic smooth muscle cells (SMCs). Copper oxidized LDL (LDL+Cu) injured the SMCs more than did native LDL. Cytotoxicity of oxidized LDL was prevented by ...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章
doi:10.5551/jat1994.1.53
更新日期:1994-01-01 00:00:00
abstract:AIMS:The present study aimed to determine the epidemiological aspects of medico-legal autopsies and manifestation of coronary atherosclerosis. METHODS:This was a cross sectional study involving 222 cases recruited from National Institute of Forensic Medicine (NIFM) Hospital Kuala Lumpur (HKL) and Department of Forensi...
journal_title:Journal of atherosclerosis and thrombosis
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doi:10.5551/jat.39107
更新日期:2018-05-01 00:00:00
abstract::We tried to investigate whether accumulation of visceral fat assessed by a simple but widely used ultrasonography was associated with common carotid atherosclerosis in women. The subjects were consecutively admitted in-patients whose body mass index was more than 22 kg/m2. Those with cardiorenal or nutritional disorde...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章
doi:10.5551/jat.9.213
更新日期:2002-01-01 00:00:00
abstract::Although many clinical studies have shown that a low testosterone level is associated with cardiovascular diseases, the role of androgens in cardiovascular physiology and pathophysiology remains controversial. Androgens exert various actions in their target organs, and the androgen receptor (AR) is widely distributed ...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章,评审
doi:10.5551/jat.31047
更新日期:2016-01-01 00:00:00
abstract:AIM:The aim of this study was to investigate the clinical effect of planned endovascular therapy (EVT) for critical limb ischemia (CLI) patients with tissue loss. Although several rounds of EVT for CLI patients are required for complete wound healing, time required for complete wound healing depends on the wound severi...
journal_title:Journal of atherosclerosis and thrombosis
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doi:10.5551/jat.45344
更新日期:2019-03-01 00:00:00
abstract:AIM:Recent evidence suggests that small dense low-density lipoprotein (sd-LDL) particles are more atherogenic than large-LDL in spite of their lower cholesterol content. This study aimed to determine whether sd-LDL-cholesterol (sd-LDL-C) is superior to LDL-C as a biomarker of coronary heart disease (CHD). METHODS:LDL ...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章
doi:10.5551/jat.e572
更新日期:2008-10-01 00:00:00
abstract:AIM:Malnutrition has been identified to be an independent predictor of morbidity and mortality in patients with chronic heart failure (CHF). However, the pathophysiological mechanisms underlying this pathway remain unclear. METHODS:Nutritional screening was performed using the controlling nutritional status (CONUT) sc...
journal_title:Journal of atherosclerosis and thrombosis
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doi:10.5551/jat.31526
更新日期:2016-06-01 00:00:00
abstract::The signaling and functions of the Endothelial Differentiation Gene (EDG) family of G protein-coupled receptors have been extensively elucidated. All the members of EDG family were shown to be receptors for lysosphingolipids or lysophospholipids. EDG-1, the prototype of EDG family receptors, is a high affinity recepto...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章,评审
doi:10.5551/jat.10.125
更新日期:2003-01-01 00:00:00
abstract:AIM:We examined the effects of mild hyperbaric oxygen on the properties of the soleus muscle in rats with metabolic syndrome. METHODS:Five-week-old metabolic syndrome (SHR/NDmcr-cp, cp/cp) rats were divided into normobaric (CP) and mild hyperbaric oxygen (CP-H) groups (n=5/group). In addition, 5-week-old Wistar rats w...
journal_title:Journal of atherosclerosis and thrombosis
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更新日期:2017-01-01 00:00:00
abstract::Statins are the main treatment for hypercholesterolemia and the cornerstone of atherosclerotic cardiovascular disease prevention. Many patients taking statins report muscle-related symptoms, one of the most important causes of statin treatment discontinuation, which is associated with an increased risk of cardiovascul...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章,评审
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更新日期:2019-03-01 00:00:00
abstract::We investigated in vitro effect of simvastatin, a potent 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, on the proliferation of human and bovine endothelial cells (EC) compared to that of bovine smooth muscle cells (SMC). Cells were cultured in a medium supplemented with 10% normal serum (FBS). S...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章
doi:10.5551/jat1994.4.102
更新日期:1998-01-01 00:00:00
abstract:AIM:Macrophage migration inhibitory factor (MIF) is known as a pro-inflammatory cytokine that regulates a broad spectrum of inflammatory reactions. MIF is expressed in vascular smooth muscle cells (VSMCs), and inhibition of the progression of atherosclerosis was observed in MIF-deficient atherosclerotic mice. However, ...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章
doi:10.5551/jat.e495
更新日期:2008-02-01 00:00:00
abstract:AIMS:Type 2 diabetes mellitus (T2DM) is no longer regarded as a coronary risk equivalent, and heterogeneity of cardiovascular risk exists, suggesting that further risk stratification should be mandatory. This study aimed to determine the prevalence and clinical predictors of coronary artery calcium (CAC) score, and eva...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章
doi:10.5551/jat.59386
更新日期:2020-11-07 00:00:00
abstract::The aim of this study was to observe the efficacy of atorvastatin and the related factors of meeting the National Cholesterol Education Program (NCEP)-recommended low-density lipoprotein (LDL) cholesterol levels in patients with hypercholesterolemia. A total of 107 patients were treated with atorvastatin 10 mg/day for...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章
doi:10.5551/jat.10.19
更新日期:2003-01-01 00:00:00
abstract:AIM:Although the InnovaTM self-expanding nitinol stent (Boston Scientific, Marlborough, MA) exhibits acceptable performance in long-term safety and efficacy when used for the treatment of femoropopliteal (FP) lesions, clinical outcomes following its implantation have not been systematically studied in real-world settin...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章,多中心研究
doi:10.5551/jat.47399
更新日期:2019-10-01 00:00:00
abstract:AIM:In Japan, heart disease and cerebral ischemic disease are major causes of death. A decrease in the level of low-density lipoprotein cholesterol (LDL-C) through intensive treatment with statins positively correlates with a reduction in the volume of plaques in patients with cardiovascular disease. The METEOR trial, ...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.5551/jat.2899
更新日期:2010-04-30 00:00:00
abstract:AIM:The Japan EPA Lipid Intervention Study (JELIS) reported a 19% reduction of the risk for coronary artery disease after long-term use of pure eicosapentaenoic acid (EPA) in Japanese patients with hypercholesterolemia. The variation in plasma fatty acid composition influenced the risk of coronary events. The aim of th...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章,随机对照试验
doi:10.5551/jat.11593
更新日期:2012-01-01 00:00:00
abstract::Inflammation and oxidative stress play key roles in atherosclerotic plaque instability, and plaque rupture/erosion and subsequent thrombus formation constitute the principal mechanisms of total vessel occlusion and acute ST-elevation myocardial infarction (STEMI). Plaque disruption triggers the formation of initial pl...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章,评审
doi:10.5551/jat.17608
更新日期:2013-01-01 00:00:00
abstract:AIM:To evaluate whether plaque scoring measurements are able to track changes in atherosclerotic plaque burden over time and to study whether this is affected by lipid-lowering therapy. METHODS:Data used were from METEOR (Measuring Effects on Intima-Media Thickness: an Evaluation Of Rosuvastatin), a randomized control...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章,随机对照试验
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更新日期:2011-01-01 00:00:00
abstract:AIM:We evaluated the impact of adherence to preferable behavior on serum lipid control assessed by a self-reported questionnaire in high-risk patients taking pravastatin for primary prevention of coronary artery disease. METHODS:High-risk patients taking pravastatin were followed for 2 years. Questionnaire surveys com...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章
doi:10.5551/jat.12682
更新日期:2012-01-01 00:00:00
abstract:AIM:We investigated the effects of a calorie-restricted low-carbohydrate diet on glucose and lipid metabolism, and body fat distribution, especially on the secretion of leptin and lipoprotein lipase from adipose tissue in Otsuka Long Evans Tokushima Fatty (OLETF) rats. METHODS:Forty-three week-old male OLETF rats were...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章
doi:10.5551/jat.e511
更新日期:2007-10-01 00:00:00
abstract:AIMS:Statins not only reduce low-density lipoprotein (LDL) cholesterol, but also prevent the progression of kidney dysfunction. Ezetimibe, a cholesterol-absorption inhibitor, also lowers LDL cholesterol levels when added to statins; however, the effect of add-on ezetimibe on kidney function has had conflicting results....
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章,随机对照试验
doi:10.5551/jat.14860
更新日期:2013-01-01 00:00:00
abstract::Rats with alloxan-induced diabetes developed severe atherosclerotic lesions when they were maintained on a 0.25% cholesterol diet for one year. The atheromatous changes developed at the aortic arch, appeared as early as 3 months after the start of the experiment, and increased thereafter. The diabetic rats also develo...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章
doi:10.5551/jat1994.1.118
更新日期:1994-01-01 00:00:00
abstract:AIMS:The identification and appropriate management of commercial motor vehicle (CMV) drivers with unrecognized obstructive sleep apnea (OSA) is a major public health concern and priority; OSA among drivers has not been fully investigated in Japan, and a better understanding of this undiagnosed disease is warranted. The...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章
doi:10.5551/jat.41806
更新日期:2018-11-01 00:00:00
abstract:AIM:To examine the impact of C-reactive protein on the risks of stroke and its subtypes, particularly among Asian populations in which median C-reactive protein levels are typically lower than in Western populations. METHODS:A prospective, nested case-control study was conducted to examine the associations between hig...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract:AIM:The ankle-brachial index(ABI) is an easy-to-use, non-invasive and reliable diagnostic tool for assessing peripheral arterial occlusive disease(PAOD). The CHADS2(congestive heart failure, hypertension, age ≧75 years, diabetes, prior stroke) score is a simple and popular clinical parameter that is used to assess the ...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章
doi:10.5551/jat.21212
更新日期:2014-01-01 00:00:00
abstract:AIM:Myriad biological effects of leptin may lead to broad therapeutic applications for various metabolic diseases, including diabetes and its complications; however, in contrast to its anorexic effect, the molecular mechanisms underlying adipopenic and glucose-lowering effects of leptin have not been fully understood. ...
journal_title:Journal of atherosclerosis and thrombosis
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doi:10.5551/jat.39552
更新日期:2017-11-01 00:00:00
abstract:AIM:Factor VII activating protease (FSAP) is a plasma serine protease involved in hemostasis and remodeling processes. Increased levels of circulating FSAP during pregnancy and in women using oral contraceptives (OCs) indicate that the hormonal status critically influences FSAP expression. In this respect, the aim of t...
journal_title:Journal of atherosclerosis and thrombosis
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doi:10.5551/jat.9589
更新日期:2012-01-01 00:00:00
abstract::Heparin cofactor II (HCII) specifically inhibits thrombin action at the site of vascular wall injury. We encountered a congenital HCII deficiency patient with advanced multiple atherosclerotic lesions. This patient led us to conduct clinical studies to examine the role of HCII against atherosclerosis. We found that th...
journal_title:Journal of atherosclerosis and thrombosis
pub_type: 杂志文章,评审
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更新日期:2009-10-01 00:00:00
abstract:AIM:Heterozygous patients with familial hypercholesterolemia (FH) are known to be associated with a high risk of coronary artery disease (CAD), which is a major determinant of their clinical outcome. The prognosis of heterozygous FH patients substantially varies, being dependent on the level of their CAD risk, and thei...
journal_title:Journal of atherosclerosis and thrombosis
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doi:10.5551/jat.11536
更新日期:2012-01-01 00:00:00