Abstract:
:Postnatal development of the mammalian heart is associated with changes in the population of isoforms of the thin filament proteins. We correlated the change in thin filament proteins, which occur in rabbit hearts between 5 days and 22 days of age, with changes in Ca2+ dependence of myofibrillar ATPase activity, force generation, and troponin C Ca2+ binding. The preparations derived from the 5-day-old animals exhibited a high molecular weight isoform of troponin T not found in the hearts of the 22-day-old animals. Other troponin T isoforms were also found to be present in different relative amounts. No other major differences in thin filament protein composition could be identified. Compared with the 5-day-old rabbit heart preparations, the ATPase activity of myofibrils from 22-day-old rabbit hearts exhibited a reduced Ca2+ sensitivity. The pCa50 (negative log of the half-maximal-activity free Ca2+) of the MgATPase activity was shifted by 0.15 pCa units with maturation. Maturation of the myofibrils was also associated with an increased effect of Mg2+ on pCa50. On increasing the Mg2+ from 2 to 10 mM at constant MgATP2-, the pCa50 of 5-day myofibrils was increased (shifted to the right) by 0.39 pCa units for 5-day-old rabbit hearts and 0.45 pCa units for 22-day-old rabbit hearts. Although similar changes in pCa50 of force developed by myofibrils were marginally significant, fibers from hearts of 5-day-old rabbits exhibited a greater Hill coefficient than hearts from 22-day-old rabbits (3.0 vs. 2.1). Despite the increased sensitivity of 5-day-old rabbit hearts to Ca2+, these hearts exhibited significantly less Ca2+ bound to myofibrillar troponin C than did the 22-day-old rabbit hearts. Moreover, the models that best described the Ca2+ binding data are different for the two age groups. Our data indicate that the Ca2+ activation and Ca2+ binding properties of myofibrillar troponin C are altered in developing cardiac myofibrils and that the changes in these properties may be influenced by changes in the troponin T isoforms present in the myofibril.
journal_name
Circ Resjournal_title
Circulation researchauthors
McAuliffe JJ,Gao LZ,Solaro RJdoi
10.1161/01.res.66.5.1204subject
Has Abstractpub_date
1990-05-01 00:00:00pages
1204-16issue
5eissn
0009-7330issn
1524-4571journal_volume
66pub_type
杂志文章abstract:RATIONALE:Abdominal aortic aneurysms (AAAs) are characterized by pathological remodeling of the aortic wall. Although both increased Krüppel-like factor 5 (KLF5) expression and macrophage infiltration have been implicated in vascular remodeling, the role of KLF5 in macrophage infiltration and AAA formation remains uncl...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.116.310367
更新日期:2017-03-03 00:00:00
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journal_title:Circulation research
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pub_type: 杂志文章,评审
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更新日期:2014-02-28 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.87.2.153
更新日期:2000-07-21 00:00:00
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更新日期:2013-07-05 00:00:00
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更新日期:2005-01-07 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.66.2.389
更新日期:1990-02-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:1976-06-01 00:00:00
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更新日期:1997-11-01 00:00:00
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更新日期:1991-05-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:1993-08-01 00:00:00
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更新日期:2003-02-21 00:00:00
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更新日期:2002-08-09 00:00:00
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pub_type: 杂志文章
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更新日期:2005-05-27 00:00:00
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pub_type: 杂志文章
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更新日期:2020-01-31 00:00:00
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pub_type: 杂志文章
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更新日期:2003-11-14 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.67.6.1381
更新日期:1990-12-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.45.5.677
更新日期:1979-11-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
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更新日期:1992-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.71.3.657
更新日期:1992-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-01-03 00:00:00
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pub_type: 杂志文章
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更新日期:2004-09-17 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.76.3.448
更新日期:1995-03-01 00:00:00
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pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2015-01-02 00:00:00
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pub_type: 杂志文章
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更新日期:1985-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-09-28 00:00:00
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pub_type: 杂志文章
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更新日期:1977-06-01 00:00:00
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更新日期:1995-09-01 00:00:00