Abstract:
:Serine proteases, such as alpha-chymotrypsin or elastase, caused an aggregation of rat ascites tumour cell lines, AH-130, AH-109A and YS, in a protein free medium which preserved the cell viability. This aggregation, which was monitored spectrophotometrically, was dependent upon the protease activities and was resistant to treatment with either a calcium chelating reagent (EDTA) or neuraminidase. However, the tumour cell aggregates were redispersed by treatment with deoxyribonuclease I (DNase I). This dispersal effect was dependent upon the DNase activity. A possible relationship between the tumour cell aggregation and development of blood-borne metastasis was studied. An intravenous inoculation in rats of tumour cell aggregates performed by the alpha-chymotrypsin treatment resulted in significantly higher numbers of lung metastatic foci than an injection of single cells. When the re-separated single cells, prepared in vitro by treatment with DNase I following alpha-chymotrypsin treatment, were injected instead of the aggregates, the enhancement of metastasis was reversed. These enhancement and reversal effects were mimicked in vivo by intravenous injections of protease and nuclease following inoculation of a single cell suspension. That is, the number of metastatic foci caused by single cell inoculation followed by an intravenous alpha-chymotrypsin injection, was higher than that in a control group receiving PBS instead of alpha-chymotrypsin. Again, this augmentation was reversed by an injection of DNase I following alpha-chymotrypsin injection. Furthermore, an injection of DNase I alone itself reduced the starting number of metastases resulting from injection of the single tumour cell suspension. These data suggest that the metastatic behaviour of tumour cells may be increased by protease inducible DNA dependent cell aggregation should it occur in the blood stream.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Sugihara S,Yamamoto T,Tsuruta J,Tanaka J,Kambara T,Hiraoka T,Miyauchi Ydoi
10.1038/bjc.1990.339subject
Has Abstractpub_date
1990-10-01 00:00:00pages
607-13issue
4eissn
0007-0920issn
1532-1827journal_volume
62pub_type
杂志文章abstract::Growth of the transplantable EMR-86 rat mammary carcinoma depends on elevated prolactin levels which are induced by oestrogenic stimulation of the pituitary. We investigated histological and cell kinetic changes during tumour regression after removal of implanted oestrogen pellets (EP), and we especially focused on th...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.232
更新日期:1996-05-01 00:00:00
abstract:BACKGROUND:Breast cancer, a heterogeneous disease has been broadly classified into oestrogen receptor positive (ER+) or oestrogen receptor negative (ER-) tumour types. Each of these tumours is dependent on specific signalling pathways for their progression. While high levels of survivin, an anti-apoptotic protein, incr...
journal_title:British journal of cancer
pub_type: 杂志文章
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更新日期:2013-03-05 00:00:00
abstract::The effect of LiCl on melanoma cell growth and differentiation was studied in mouse and human melanoma cell lines. LiCl markedly inhibited B16 and HT-144 melanoma cell growth in vitro. Clonogenicity in soft agar of the melanoma cells was also markedly inhibited by LiCl. Pretreatment of B16 mouse melanoma cells with Li...
journal_title:British journal of cancer
pub_type: 杂志文章
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更新日期:1987-01-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
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更新日期:1989-08-01 00:00:00
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journal_title:British journal of cancer
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更新日期:2009-01-13 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1993.46
更新日期:1993-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1038/bjc.2014.595
更新日期:2015-01-20 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1993.97
更新日期:1993-03-01 00:00:00
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更新日期:2015-12-22 00:00:00
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pub_type: 杂志文章
doi:10.1038/sj.bjc.6690136
更新日期:1999-02-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章,随机对照试验
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更新日期:2012-02-14 00:00:00
abstract:BACKGROUND:Lapatinib, a dual EGFR and HER2 inhibitor has shown disappointing results in clinical trials of metastatic oesophago-gastric adenocarcinomas (OGAs), and in vitro studies suggest that MET, IGFR, and HER3 confer resistance. This trial applied Lapatinib in the curative neoadjuvant setting and investigated the f...
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pub_type: 临床试验,杂志文章,多中心研究
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更新日期:2015-11-03 00:00:00
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pub_type: 杂志文章
doi:10.1038/sj.bjc.6605923
更新日期:2010-11-09 00:00:00
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pub_type: 杂志文章
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更新日期:2011-09-06 00:00:00
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pub_type: 杂志文章,随机对照试验
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更新日期:2017-03-14 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1989.337
更新日期:1989-11-01 00:00:00
abstract::It has been postulated that paternal gonadal exposure would increase the sex ratio by inducing X-chromosomal dominant lethals but that maternal gonadal exposure would decrease the sex ratio by inducing recessive sex-linked lethals. We therefore evaluated the sex ratio (male-to-female ratio) of children born to survivo...
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更新日期:2003-02-10 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1986.168
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pub_type: 杂志文章
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更新日期:2014-09-09 00:00:00
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更新日期:2017-08-08 00:00:00
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更新日期:2006-02-13 00:00:00
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journal_title:British journal of cancer
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更新日期:2000-12-01 00:00:00
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更新日期:2008-01-15 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2015-12-01 00:00:00