Abstract:
:In a family, a moderate bleeding disorder in two patients has been specified as Glanzmann's thrombasthenia because of characteristic defects in platelet function. Analysis of platelet membrane glycoproteins revealed about a 50% decrease in the amount of GP IIb-IIIa complex (α(11b)β(3) integrin), which appeared normal with respect to electrophoretic mobility, apparent M, and isoelectric behaviour of GP IIb and GP IIIa. Content of platelet fibrinogen (Fg) was normal. [(125)I]Fg binding to ADP-stimulated platelets was strongly reduced but K(d) values indicated a much higher affinity of the residual receptors for both [(125)I]Fg and RGD peptide. Fg bound to the isolated complex as detected by crossed immunoelectrophoresis and there was substantial expression of endogenous Fg on the surface of washed thrombin-stimulated platelets. RGD-peptide induced increased binding of conformation-specific monoclonal antibodies (Mabs) LIBS 1 and PMI-1. Flow cytometric analysis revealed defective binding of nine Mabs, among them two out of three tested antibodies specific for GP IIIa (C 17, AP 5). Results indicate a genetic variant of GP IIb-IIIa complex with the structural abnormality possibly related to defective conformational change upon activation.
journal_name
Plateletsjournal_title
Plateletsauthors
Meyer M,Thieme A,Jablonka B,Just M,Ströhl C,Schellenberg I,Kirchmaier CMdoi
10.3109/09537109609023581subject
Has Abstractpub_date
1996-01-01 00:00:00pages
215-24issue
4eissn
0953-7104issn
1369-1635journal_volume
7pub_type
杂志文章相关文献
PLATELETS文献大全abstract::Mechanisms involved in the reduction of blood platelet functions by various plant extract, including the grape seeds extract (rich in phenolic compounds, a mixture of about 95% oligomeric phenols; GSE) are still unclear. In the literature there are few papers describing studies on the effects of GSE on selected elemen...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2011.618562
更新日期:2012-01-01 00:00:00
abstract::The ADP receptor P2Y12, the thromboxane A2 receptor (TXA2R) and the C-type lectin-like receptor 2 (CLEC-2) mediate platelet activation by different mechanisms. Only little is known about the expression of the receptors in human megakaryopoiesis. Our study aimed to establish a flow cytometry (FC) method for the measure...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1782868
更新日期:2020-07-03 00:00:00
abstract::Adenosine diphosphate (ADP) is recognized as an important mediator of platelet aggregation. Transient aggregation at low (< or =1 microM), and sustained aggregation at higher ADP concentrations are consistently observed. Dissociation of platelet aggregates has been described and may explain the reversible component of...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100120071004
更新日期:2001-08-01 00:00:00
abstract::A platelet substitute, Synthocytes, is being developed for the prevention and treatment of thrombocytopenia. Synthocytes are composed of fibrinogen adsorbed on heat stabilised albumin microcapsules of defined size. The purpose of this study was to perform experiments in vitro to investigate the capacity of Synthocytes...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/0953371027000
更新日期:2002-06-01 00:00:00
abstract::Familial bleeding problems are frequently difficult to diagnose because currently used clinical tests cannot identify intracellular molecular defects of platelets. Using platelet proteomics, a comprehensive analytical tool, we diagnosed a family with severe bleeding problems of unknown origin with Quebec Platelet Diso...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100802010547
更新日期:2008-08-01 00:00:00
abstract::Tissue factor (TF) is the most important initiator of intravascular coagulation. This article will review published evidence on the contribution of platelets to TF exposure to the circulating blood. The following mechanisms will be discussed: decryption of monocyte TF by platelets, contribution of platelets to TF expr...
journal_title:Platelets
pub_type: 杂志文章,评审
doi:10.1080/09537100500140190
更新日期:2005-09-01 00:00:00
abstract::Vascular diseases such as myocardial infarction and ischemic stroke are associated with increased platelet function whilst the risk of recurrence is reduced by antiplatelet agents such as aspirin, clopidogrel, and prasugrel. However, some patients exhibit high platelet reactivity, especially with clopidogrel. Existing...
journal_title:Platelets
pub_type: 杂志文章,评审
doi:10.1080/09537104.2018.1445839
更新日期:2018-07-01 00:00:00
abstract::In patients with acute coronary syndrome (ACS), treatment using ticagrelor demonstrated significant ischemic benefits over clopidogrel; however, it was associated with increased bleeding complications leading to frequent de-escalation to clopidogrel. The objective of the present study was to investigate the efficacy a...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2019.1609668
更新日期:2020-01-01 00:00:00
abstract::The purpose of the study was to evaluate safety, effects on platelet aggregation and pharmacokinetics of F(ab')(2) fragments of anti-glycoprotein (GP) IIb-IIIa murine monoclonal antibody FRaMon (F(ab')(2) FRaMon) upon its intravenous administration in patients undergoing high-risk coronary angioplasty. Patients were t...
journal_title:Platelets
pub_type: 临床试验,杂志文章
doi:10.1080/0953710021000057839
更新日期:2002-12-01 00:00:00
abstract::High on-treatment platelet reactivity (HTPR) is associated with poor prognosis in patients undergoing percutaneous coronary intervention (PCI). The antiplatelet effect and safety of prasugrel was compared to that of double-dose clopidogrel in patients with stable coronary artery disease or acute coronary syndrome (ACS...
journal_title:Platelets
pub_type: 杂志文章,随机对照试验
doi:10.3109/09537104.2013.845874
更新日期:2014-01-01 00:00:00
abstract::Fibrin glue is a promising alternative for low-output enterocutaneous fistula closure. Bacterial flora colonizing inside the fistula tract, however, may limit the glue application. Single-donor-derived, platelet-rich materials were hypothesized in this study to have antimicrobial activity against Gram-negative microor...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2012.761685
更新日期:2013-01-01 00:00:00
abstract::Intracranial hemorrhage (ICH) is a devastating complication of immune thrombocytopenia (ITP). However, information on ICH in ITP patients under the age of 60 years is limited, and no predictive tools are available in clinical practice. A total of 93 adult patients with ITP who developed ICH before 60 years of age were...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1786042
更新日期:2020-07-02 00:00:00
abstract::Thrombocytopenia is a clinically relevant outcome in HIV. However, the epidemiology of this condition, including frequency, severity, and duration, has not been well-characterized in the era of highly active antiretroviral therapy (HAART). In this study, we describe the epidemiology of thrombocytopenia using two metho...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2011.582526
更新日期:2011-01-01 00:00:00
abstract::A number of hematopoietic growth factors and other cytokines are capable of altering platelet production and function. Enhancement of these processes may be exploited to ameliorate bleeding propensity in thrombocytopenic patients. Under certain circumstances, cytokines may have adverse effects on the hemostatic system...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537109709169324
更新日期:1997-01-01 00:00:00
abstract::The aim of this study was to determine the contribution of increased PGD(2) formation to the anti-aggregatory action of a thromboxane A(2) (TXA(2)) synthase inhibitor and TP receptor antagonist (ZD1542) against collagen-induced human platelet aggregation in citrated whole blood using the prostaglandin (PG) DP receptor...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109409005529
更新日期:1994-01-01 00:00:00
abstract::To study the possibility of using thrombin generation tests in platelet-rich and platelet-poor plasma for evaluation of dual antiplatelet therapy efficacy in patients with coronary artery disease (CAD), following percutaneous coronary intervention. Venous blood was analyzed from CAD patients aged 53-75 years who had u...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2017.1294680
更新日期:2018-03-01 00:00:00
abstract::Oxidative damage to lipids in plasma, blood platelets and neurons in patients with schizophrenia was described. The aim of our present study was to evaluate oxidative/nitrative modifications of blood platelets proteins by measurement the level of biomarkers of oxidative stress such as carbonyl groups, thiol groups and...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100802641499
更新日期:2009-03-01 00:00:00
abstract::SIRT1, a class III histone deacetylase, is critically involved in cellular response to stress and modulates cardiovascular risk factors. However, its role in thrombus formation is largely unknown. Thus, this study investigated the effect of SIRT1 on pulmonary thrombus formation, and then identified its role in the mod...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2016.1190005
更新日期:2016-12-01 00:00:00
abstract::Although (very) late coronary stent thrombosis is a rare complication after percutaneous coronary interventions (PCI), its consequences are devastating with a high morbidity and mortality. Previous studies have identified several clinical, procedural and angiographic characteristics that are associated with stent thro...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537100903388367
更新日期:2010-01-01 00:00:00
abstract::The distribution of some proteoglycan anionic sites and carbohydrate moieties on the diabetic platelet surface has been investigated by cytochemical methods. Human blood platelets obtained from either diabetic patients (serum glycaemia 9.6-19.2 mmol/L) or normal donors were labeled with specific stains (Ruthenium Red,...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109409006047
更新日期:1994-01-01 00:00:00
abstract::Patients with thrombocytopenia or platelet disorders are at risk of severe bleeding. We report the development and validation of flow cytometry assays to diagnose platelet disorders and to assess platelet function independently of platelet count. The assays were developed to measure glycoprotein levels (panel 1) and p...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2015.1032919
更新日期:2016-01-01 00:00:00
abstract::In previous studies we have shown that relaxin (RLX), a peptide hormone produced predominantly during pregnancy, is a powerful inhibitor of platelet aggregation. The current study shows that RLX, given systemically to rats, also affects the number of circulating platelets and their release by spleen megakaryocytes. Ma...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109509078467
更新日期:1995-01-01 00:00:00
abstract::Studies on markers for bleeding risk among thrombocytopenic cancer patients are lacking. This prospective observational cohort study investigated whether platelet parameters and a standardised bleeding questionnaire predicted bleeding or prophylactic platelet transfusions in patients with cancer and thrombocytopenia. ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2015.1131814
更新日期:2016-09-01 00:00:00
abstract::Strenuous physical exercise leads to platelet activation that is normally counterbalanced by the production of endothelium-derived anti-platelet mediators, including prostacyclin (PGI2) and nitric oxide (NO). However, in the case of endothelial dysfunction, e.g. in atherosclerosis, there exists an increased risk for i...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2016.1254764
更新日期:2017-11-01 00:00:00
abstract::Previous investigations from our laboratory identified the ultrastructural pathology and cytochemistry of macrothrombocytes (MTC) from patients with the X-linked, G208S varient of the GATA-1 mutation.A subsequent biochemical study of the MTC cytoskeletal proteins using polyacrylamide gel electrophoresis and western bl...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100902818344
更新日期:2009-05-01 00:00:00
abstract::Lipopolysaccharide (LPS, endotoxin) is a well-known causative agent of septic shock and disseminated intravascular coagulation. The action of LPS is related to the activation of many types of cells, including blood platelets. We examined the effects of different LPSs from Proteus mirabilis (smooth form LPS S1959 and r...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/0953710021000024385
更新日期:2002-11-01 00:00:00
abstract::The risk and benefit of GP-IIb/IIIa Inhibition (GPI) in combination with recent antiplatelet regimens in acute coronary syndromes (ACS) remain unassessed. The advent of fast-acting highly active oral P2Y12 inhibitors questions the additional value and risk of their association with GPI. We studied the effect of GPI in...
journal_title:Platelets
pub_type: 杂志文章,meta分析,评审
doi:10.1080/09537104.2016.1218453
更新日期:2017-03-01 00:00:00
abstract::Postoperative atrial fibrillation (AF) is a common complication of coronary artery bypass grafting (CABG). The mean platelet volume (MPV) is an important marker of platelet activity and is associated with cardiovascular risk factors. We investigated whether the MPV is associated with the development of AF after CABG. ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2013.767443
更新日期:2014-01-01 00:00:00
abstract::The present study was performed to explore differences in the expression of P-selectin and IL-8 production of blood cells in healthy individuals with large size platelets (MI-families) as compared to people having normal size platelets. A positive correlation between LPS-induced IL-8 production per platelet in whole b...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/02697450310001632597
更新日期:2003-11-01 00:00:00
abstract::Bleeding in the setting of acute coronary syndromes (ACS) has negative prognostic implications. We sought to determine the impact of different levels of on-treatment platelet reactivity (PR) to ADP on both bleeding and ischemic events in ACS patients receiving coronary stenting. PR to ADP was measured with the VerifyN...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2011.568075
更新日期:2011-01-01 00:00:00