Abstract:
:A dual isotype (IgG, IgA) enzyme-linked immunosorbent assay (ELISA) designed to provide enhanced detection of primary biliary cirrhosis (PBC)-specific autoantibodies against both major mitochondrial and nuclear antigens has been developed and recently become commercially available. The assay (PBC Screen) simultaneously detects IgG and IgA autoantibodies to the immunodominant portions of the 3 major mitochondrial (MIT3) and nuclear (gp210, and sp100) antigens. The aim of this study was to compare the performance of the PBC Screen to the combined performance obtained with individual IgG ELISAs to MIT3, gp210, and sp100 on a large group of selected patients from multiple centers. A total of 1175 patients with PBC and 1232 subjects without PBC were evaluated. Non-PBC groups included healthy controls (624) as well as individuals with autoimmune hepatitis (281), primary sclerosing cholangitis (77), viral hepatitis (91 hepatitis B and 98 hepatitis C), other liver diseases (31), and other infectious or autoimmune diseases (30). The PBC Screen at the receiver operator characteristic optimized cutoff of 27.8 units, had an overall sensitivity of 83.8%, specificity of 94.7% and area under curve of 0.9212. This was similar to the specificity of 96.1% obtained by the combined results of individual MIT3, sp100, and gp210 IgG ELISAs (kappa index at 0.898). Of the 253 PBC patients without AMA detectable by immunofluorescence, 113 (44.7%) were interpreted as positive for PBC-specific autoantibodies. In conclusion, the PBC Screen is an appropriate first-line test for the diagnosis of PBC, including for patients negative for markers assessed using conventional methods.
journal_name
J Autoimmunjournal_title
Journal of autoimmunityauthors
Liu H,Norman GL,Shums Z,Worman HJ,Krawitt EL,Bizzaro N,Vergani D,Bogdanos DP,Dalekos GN,Milkiewicz P,Czaja AJ,Heathcote EJ,Hirschfield GM,Tan EM,Miyachi K,Bignotto M,Battezzati PM,Lleo A,Leung PS,Podda M,Gershwindoi
10.1016/j.jaut.2010.09.005subject
Has Abstractpub_date
2010-12-01 00:00:00pages
436-42issue
4eissn
0896-8411issn
1095-9157pii
S0896-8411(10)00104-6journal_volume
35pub_type
杂志文章abstract::Genetic polymorphism in MECP2/IRAK1 on chromosome Xq28 is a confirmed and replicated susceptibility locus for lupus. High linkage disequilibrium in this locus suggests that both MECP2 and IRAK1 are candidate genes for the disease. DNA methylation changes in lupus T cells play a central role in the pathogenesis of lupu...
journal_title:Journal of autoimmunity
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journal_title:Journal of autoimmunity
pub_type: 杂志文章,评审
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更新日期:1993-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-12-01 00:00:00
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journal_title:Journal of autoimmunity
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journal_title:Journal of autoimmunity
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journal_title:Journal of autoimmunity
pub_type: 杂志文章,评审
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更新日期:2000-09-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1006/jaut.2001.0547
更新日期:2001-12-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1016/j.jaut.2020.102401
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1006/jaut.1995.0008
更新日期:1995-02-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1016/j.jaut.2009.05.003
更新日期:2010-03-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1016/j.jaut.2006.12.002
更新日期:2006-12-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2010-11-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1016/j.jaut.2004.01.005
更新日期:2004-06-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1006/jaut.1995.0047
更新日期:1995-10-01 00:00:00
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pub_type: 杂志文章,评审
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pub_type: 杂志文章,随机对照试验
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pub_type: 杂志文章
doi:10.1016/0896-8411(91)90055-h
更新日期:1991-12-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章,评审
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doi:10.1016/j.jaut.2013.11.005
更新日期:2014-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.jaut.2009.03.011
更新日期:2009-11-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章,评审
doi:10.1016/j.jaut.2011.11.002
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1016/j.jaut.2010.07.002
更新日期:2010-12-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章,评审
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1016/j.jaut.2011.07.003
更新日期:2011-12-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1006/jaut.1993.1040
更新日期:1993-08-01 00:00:00