Abstract：
:A simple procedure for isolating a fraction enriched in synaptic plasma membrane (SPM) vesicles from sheep brain cortex is described and is used in studying uptake and release of [(3)H]GABA. The crude synaptosomal fraction was subjected to osmotic shock and the lysate was subfractioned in a discontinuous density gradient of dextran T110. The activities of the enzyme markers of SPM, the phospholipid analysis and electron microscopy examination suggest that this fraction of SPM is of a purity similar to that of preparations previously described by more time consuming methods. Superfusion of SPM vesicles with sucrose, ?-aminobutyric acid (GABA), veratridine, increased [K(+)](o), or reduced [Cl(?)](o), induced the release of 4-amino-n-[2,3,(3)H]butyric acid ([(3)H]GABA) previously loaded into these vesicles by a Na(+), Cl(?) and K(+) dependent mechanism. Spontaneous and depolarization induced release of [(3)H]GABA does not require external Na(+) or Cl(?), whereas the release elicited by unlabeled GABA (homoexchange) is stimulated by Na(+) or Cl(?). Thus, both the uptake and homoexchange of [(3)H]GABA are influenced by Na(+) and Cl(?).
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Gonçalves PP,Santos MS,Carvalho APdoi
10.1016/0197-0186(90)90022-lsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
401-13issue
3eissn
0197-0186issn
1872-9754pii
0197-0186(90)90022-Ljournal_volume
17pub_type
杂志文章abstract：:In this review, I summarized transition in Dr. Marshall Nirenberg's research interests during 1970s, from a view of a long-lasting collaborator. Nirenberg switched his research filed to neurobiology after his success in deciphering genetic code and being honored with the Nobel Prize in Physiology or Medicine in 1968. ...
journal_title：Neurochemistry international
pub_type： 传,历史文章,杂志文章
doi：10.1016/j.neuint.2012.02.030
更新日期：2012-11-01 00:00:00
abstract：:Alpha-synuclein (ASN), a 140-amino acid protein, is richly expressed in presynaptic terminals in the central nervous system, where it plays a role in synaptic vesicle function. However, if it is altered and accumulated it is involved in neurodegeneration as Parkinson's disease (PD). ASN contained 35-amino acid domain ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2006.01.025
更新日期：2006-09-01 00:00:00
abstract：:Primary cultures of cerebral cortical astrocytes and neurons, as well as neurons growing on top of the astrocytes (sandwich co-cultures), were incubated with 1-[13C]glucose or 2-[13C]acetate and in the presence or absence of the glutamine synthetase inhibitor methionine sulfoximine. [13C]NMR spectroscopy at 125 MHz wa...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(93)90064-c
更新日期：1993-01-01 00:00:00
abstract：:Using a consistent, reproducible and reliable cortical focal ischemia in rat (permanent unilateral occlusion of the left middle cerebral artery & the ipsilateral common carotid artery [MCAo + CCAo] with a 1 h temporary occlusion of the contralateral CCA), the levels of four major membrane fatty acids (palmitic, C16:0;...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(93)90094-l
更新日期：1993-08-01 00:00:00
abstract：:Neuronal cell death is often triggered by events that involve intracellular increases in Ca2+. Under resting conditions, the intracellular Ca2+ concentration is tightly controlled by a number of extrusion and sequestering mechanisms involving the plasma membrane, mitochondria, and ER. These mechanisms act to prevent a...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/j.neuint.2017.03.010
更新日期：2017-10-01 00:00:00
abstract：:A significant increase in the number of measurable [3H]Ro 5-4864 receptors was found in forebrain membranes of chicks submitted to 15 min of acute swim stress compared to non-stressed chicks. In addition, low subsolubilizing concentrations of Triton X-100 caused a significant increase in the measurable [3H]Ro 5-4864 r...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(95)00093-3
更新日期：1996-04-01 00:00:00
abstract：:Monosialoganglioside (GM1) is a glycosphingolipid present in most cell membranes that displays antioxidant and neuroprotective properties. It has been recently described that GM1 induces vasodilation. However, the mechanisms underlying GM1-induced vasodilation were not evaluated to date. Therefore, in this study we in...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2008.07.011
更新日期：2008-12-01 00:00:00
abstract：:Ras homolog enriched in the brain (Rheb) is a homolog of Ras GTPase that regulates cell growth, proliferation, and cell cycle via mammalian target of rapamycin (mTOR). Recently, it has been confirmed that Rheb activation not only promotes cellular proliferation and differentiation but also enhances cellular apoptosis ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2013.01.025
更新日期：2013-03-01 00:00:00
abstract：:A sandwich enzyme immunoassay (EIA) was established by using purified human serum dopamine-?-hydroxylase (DBH) as a standard protein and a monospecific polyclonal antibody raised against human DBH purified from human pheochromocytoma. The EIA was applied to measuring DBH levels in human CSF from Parkinsonian patients ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(88)90126-x
更新日期：1988-01-01 00:00:00
abstract：:Tissue preparations from the brain of rats and guinea-pigs were examined for their activity in binding isotopically labelled colchicine, as a measure of their content of tubulin. The amount of binding material extracted into a cold buffered solution was unaffected by keeping tissues at 0 degrees C. It diminished by on...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(83)90072-4
更新日期：1983-01-01 00:00:00
abstract：:Receptors for transmitters, as varied as those expressed by neurons, have been described on primary astrocyte cultures prepared from new-born rats and mice. A variety of functional effects and considerable cell-to-cell and regional heterogeneity have been observed for such receptors in vitro. The various systems avail...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/0197-0186(94)00118-e
更新日期：1995-01-01 00:00:00
abstract：:Allosteric modulation of [3H]strychnine binding to glycine receptors (GlyRs) was examined in synaptosomal membranes of rat spinal cord. An allosteric model enabled us to determine the cooperativity factors of the allosteric agents with [3H]strychnine and glycine bindings (alpha and beta, respectively). We modified the...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2003.08.010
更新日期：2004-06-01 00:00:00
abstract：:Individual analysis of synaptic terminals calcium responses, induced by dinucleotides pentaphosphate, Ap(5)A or Gp(5)G, demonstrates the presence of two main groups considering the concentration required for stimulation. The first group corresponds to those responding to Ap(5)A or Gp(5)G at nanomolar concentration, re...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2006.12.007
更新日期：2007-03-01 00:00:00
abstract：:The venom secreted from glands appended to the jaws of Glycera convoluta, a Polychaete Annelid, increases the spontaneous quantal release of transmitter from nerve terminals. The component that is biologically active on vertebrate cholinergic nerve terminals has recently been shown to be a high molecular weight protei...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(85)90009-9
更新日期：1985-01-01 00:00:00
abstract：:Serotonin2C receptor (5-HT2C) agonists are promising drugs for the treatment of neuropsychiatric diseases. However, their effect is not completely understood in part because they possibly affect several neurobiological networks simultaneously. We studied the effect of the 5-HT2C receptor agonist WAY-163909 (0.3 and 3 ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2019.01.019
更新日期：2019-03-01 00:00:00
abstract：:D1 and D2 receptors are key mediators of dopaminergic signaling in the brain, and since the manifestations of pathologies related to dopamine are different in female and male patients, it is important to analyze if there are sex-related differences in dopaminergic markers. To contribute to the knowledge in this regard...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2016.09.001
更新日期：2016-11-01 00:00:00
abstract：:Primary cortical neurons exposed to the mitochondrial toxin NaN3 (0.1-3 mM) were submitted to oxidative stress with H2O2 (30-150 μM), to mimic conditions observed in neurodegenerative disorders. The effects of such treatment on a series of parameters useful in characterizing neuronal damage were investigated: (i) the ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2013.05.005
更新日期：2013-08-01 00:00:00
abstract：:We have investigated effects of continuous SSRI administration and abrupt discontinuation on biochemical and behavioral indices of rat brain serotonin function, and attempted to identify underlying mechanisms. Biochemistry of serotonin was assessed with brain tissue assays and microdialysis; behavior was assessed as t...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2010.10.001
更新日期：2010-12-01 00:00:00
abstract：:Rat corticoencephalic cell cultures were investigated by high performance liquid chromatography for changes in the levels of adenosine 5'-triphosphate (ATP), guanosine 5'-triphosphate (GTP), uridine 5'-triphosphate (UTP), cytidine 5'-triphosphate (CTP), and the respective nucleoside diphosphates. Hypoxia was induced b...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(01)00102-4
更新日期：2002-04-01 00:00:00
abstract：:The 12/15-lipoxygenase (12/15-LOX) enzymes react with polyunsaturated fatty acids producing active lipid metabolites that are involved in plethora of human diseases including neurological disorders. A great many of elegant studies over the last decades have contributed to unraveling the mechanism how 12/15-lipoxygenas...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/j.neuint.2018.04.002
更新日期：2018-09-01 00:00:00
abstract：:Lipid peroxidation has many deleterious effects on cells, and in the nervous system is considered to be involved in the pathogenesis of neurodegenerative diseases. To suppress lipid peroxidation, cells have various defense systems such as glutathione and thioredoxin, and defects in these defense systems will result in...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2010.04.015
更新日期：2010-09-01 00:00:00
abstract：:Monocarboxylate transporter (MCT1) levels in brains of adult Long-Evans rats on a high-fat (ketogenic) diet were investigated using light and electron microscopic immunocytochemical methods. Rats given the ketogenic diet (91% fat and 9% protein) for up to 6 weeks had increased levels of the monocarboxylate transporter...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(00)00102-9
更新日期：2001-05-01 00:00:00
abstract：:Glycine transporter GlyT1 plays important role in maintaining accurate glycine concentration in local brain microenvironment. Transporting efficiency of GlyT1 is strongly affected by the state of its distal C-terminus, which regulates transporter trafficking and cellular surface density. Using selected range of antibo...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2010.06.003
更新日期：2010-10-01 00:00:00
abstract：:Variants in the gene encoding the enzyme glutamic acid decarboxylase like 1 (GADL1) have been associated with response to lithium therapy. Both GADL1 and the related enzyme cysteine sulfinic acid decarboxylase (CSAD) have been proposed to be involved in the pyridoxal-5'-phosphate (PLP)-dependent biosynthesis of taurin...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2015.08.013
更新日期：2015-11-01 00:00:00
abstract：:Thyrotropin releasing hormone (TRH) present in several brain areas has been proposed as a neuromodulator. Its administration produces opposite effects to those observed with acute ethanol consumption. Opioid peptides, in contrast, have been proposed to mediate some of the effects of alcohol intoxication. We measured T...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(00)00059-0
更新日期：2000-11-01 00:00:00
abstract：:Neuroinflammation is an important factor contributing to cognitive impairment and neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease (PD), Amyotrophic lateral sclerosis (ALS), ischemic injury, and multiple sclerosis (MS). These diseases are characterized by inexorable progressive injur...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/j.neuint.2020.104714
更新日期：2020-06-01 00:00:00
abstract：:Air pollution is an important contributor to the global burden of disease, particularly to respiratory and cardiovascular diseases. In recent years, evidence is accumulating that air pollution may adversely affect the nervous system as shown by human epidemiological studies and by animal models. Age appears to play a ...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/j.neuint.2019.104580
更新日期：2019-12-01 00:00:00
abstract：:Marked age-related changes are noted in the neurotensin distribution of the human hippocampus. Different neuronal structures containing neurotensin are detected by immunohistochemistry during postnatal brain growth from birth to 4 years but no later. They are the neurotensin-immunoreactive pyramidal cells of the subic...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(89)90114-9
更新日期：1989-01-01 00:00:00
abstract：:Phosphodiesterase 7B (PDE7B) inhibition has been considered as a therapeutic target for the treatment of several neurological disorders. Currently, there are no radio-labeled tracers available to determine receptor occupancy (RO) of this target. Developing such a tracer could greatly facilitate the identification of v...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2020.104735
更新日期：2020-07-01 00:00:00
abstract：:The system of GABA transporters in neural cells constitutes an efficient mechanism for terminating inhibitory GABAergic neurotransmission. As such these transporter are important therapeutical targets in epilepsy and potentially other neurological diseases related to the GABA system. In this study a number of analogs ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(03)00033-0
更新日期：2003-09-01 00:00:00
